1,886 research outputs found
Cycloaddition Reactions of the Diphosphenyl Complex (η5-C5Me5)(CO)2Fe-P=P-Mes* (Mes* = 2,4,6-tBu3C6H2) with Hexafluoroacetone. X-Ray Structure Analyses of (η5-C5Me5)(CO) Fe P(=PMes*)OC(CF3)2CO and (η5-C5Me5)(CO)2FePP(Mes*)OC(CF3)2
Weber L, Buchwald S, Lentz D, Preugschat D, Stammler H-G, Neumann B. Cycloaddition Reactions of the Diphosphenyl Complex (η5-C5Me5)(CO)2Fe-P=P-Mes* (Mes* = 2,4,6-tBu3C6H2) with Hexafluoroacetone. X-Ray Structure Analyses of (η5-C5Me5)(CO) Fe P(=PMes*)OC(CF3)2CO and (η5-C5Me5)(CO)2FePP(Mes*)OC(CF3)2. Organometallics. 1992;11(7):2351-2353.The diphosphenyl complex (eta-5-C5Me5)-(CO)2Fe-P=P-Mes* (Mes* = 2,4,6-tBu3C6H2) undergoes a [3 + 2] dipolar cycloaddition with hexafluoroacetone to give the metalla heterocycle (eta-5-C5Me5)(CO)-Fe-P(=PMes*)OC(CF3)2C(O) with a remarkably short Fe-P bond (2.084 (4) angstrom) and an exocyclic P=P bond. When stored in solution at -40-degrees-C, this complex partly rearranges to the metalated 1-oxa-2,3-diphosphetane (eta-5-C5Me5)(CO)2Fe-P-P(Mes*)OC(CF3)2. The molecular structures of both isomers were elucidated by single-crystal X-ray analyses
Highly diverse and antimicrobial susceptible Escherichia coli display a naïve bacterial population in fruit bats from the Republic of Congo
Bats are suspected to be a reservoir of several bacterial and viral pathogens
relevant to animal and human health, but studies on Escherichia coli in these
animals are sparse. We investigated the presence of E. coli in tissue samples
(liver, lung and intestines) collected from 50 fruit bats of five different
species (Eidolon helvum, Epomops franqueti, Hypsignathus monstrosus,
Myonycteris torquata, Rousettus aegyptiacus) of two different areas in the
Republic of Congo between 2009 and 2010. To assess E. coli pathotypes and
phylogenetic relationships, we determined the presence of 59 virulence
associated genes and multilocus sequence types (STs). Isolates were further
tested for their susceptibility to several antimicrobial substances by agar
disk diffusion test and for the presence of an Extended-Spectrum Beta-
Lactamase phenotype. E. coli was detected in 60% of the bats analysed. The
diversity of E. coli strains was very high, with 37 different STs within 40
isolates. Occasionally, we detected sequence types (e.g. ST69, ST127, and
ST131) and pathotypes (e.g. ExPEC, EPEC and atypical EPEC), which are known
pathogens in human and/or animal infections. Although the majority of strains
were assigned to phylogenetic group B2 (46.2%), which is linked with the ExPEC
pathovar, occurrence of virulence-associated genes in these strains were
unexpectedly low. Due to this, and as only few of the E. coli isolates showed
intermediate resistance to certain antimicrobial substances, we assume a
rather naïve E. coli population, lacking contact to humans or domestic
animals. Future studies featuring in depth comparative whole genome sequence
analyses will provide insights into the microevolution of this interesting
strain collection
Successful treatment of granulomatous reactions secondary to injection of esthetic implants
In recent years, various injectable materials have come into use to
improve esthetic appearance. OBJECTIVE: We describe the clinical and
histopathologic aspects of two patients who received intradermal injections of an
unknown dermal filler and the different diagnostic tools used to identify the
unknown injected material (reflexion electron microscopy, electron dispersing
x-ray) and discuss the possibility of a metastatic granulomatous reaction in one
patient. We also describe two treatments for this complication and evaluate the
legal considerations of the use of materials that have been adulterated and/or
whose composition is unknown to the patient. METHODS: We present two patients who
developed a granulomatous foreign-body reaction after the subcutaneous injection
of an esthetic implant. We treated patient 1 with isotretinoin and 2 months later
with doxycycline. We administered isotretinoin to patient 2. RESULTS: We observed
a partial improvement in patient 1 after isotretinoin treatment and a remarkable
improvement after administration of doxycycline. In patient 2, we observed an
excellent response to isotretinoin. CONCLUSION: Isotretinoin and doxycycline,
when administered separately, seem to offer effective treatment for reactions
resulting from silicone implants. However, further studies that include a larger
number of patients and those with reactions secondary to other fillers are
clearly needed before the effectiveness of this treatment can be confirmed
Metabolomic and transcriptomic stress response of Escherichia coli
GC-MS-based analysis of the metabolic response of Escherichia coli exposed to four different stress conditions reveals reduction of energy expensive pathways.Time-resolved response of E. coli to changing environmental conditions is more specific on the metabolite as compared with the transcript level.Cease of growth during stress response as compared with stationary phase response invokes similar transcript but dissimilar metabolite responses.Condition-dependent associations between metabolites and transcripts are revealed applying co-clustering and canonical correlation analysis
HLA class I-restricted MYD88 L265P-derived peptides as specific targets for lymphoma immunotherapy
Genome sequencing has uncovered an array of recurring somatic mutations in different non-Hodgkin lymphoma (NHL) subtypes. If affecting protein-coding regions, such mutations may yield mutation-derived peptides that may be presented by HLA class I proteins and recognized by cytotoxic T cells. A recurring somatic and oncogenic driver mutation of the Toll-like receptor adaptor protein MYD88, Leu265Pro (L265P) was identified in up to 90% of different NHL subtype patients. We therefore screened the potential of MYD88(L265P)-derived peptides to elicit cytotoxic T cell responses as tumor-specific neoantigens. Based on in silico predictions, we identified potential MYD88(L265P)-containing HLA ligands for several HLA class I restrictions. A set of HLA class I MYD88(L265P)-derived ligands elicited specific cytotoxic T cell responses for HLA-B*07 and -B*15. These data highlight the potential of MYD88(L265P) mutation-specific peptide-based immunotherapy as a novel personalized treatment approach for patients with MYD88(L265P+) NHLs that may complement pharmacological approaches targeting oncogenic MyD88 L265P signaling
C,C′-Bis(benzodiazaborolyl)dicarba-closo-dodecaboranes: Synthesis, structures, photophysics and electrochemistry
In situ observations of the Swiss periglacial environment using GNSS instruments
Monitoring of the periglacial environment is relevant for many disciplines including glaciology, natural hazard management, geomorphology, and geodesy. Since October 2022, Rock Glacier Velocity (RGV) is a new Essential Climate Variable (ECV) product within the Global Climate Observing System (GCOS). However, geodetic surveys at high elevation remain very challenging due to environmental and logistical reasons. During the past decades, the introduction of low-cost global navigation satellite system (GNSS) technologies has allowed us to increase the accuracy and frequency of the observations. Today, permanent GNSS instruments enable continuous surface displacement observations at millimetre accuracy with a sub-daily resolution. In this paper, we describe decennial time series of GNSS observables as well as accompanying meteorological data. The observations comprise 54 positions located on different periglacial landforms (rock glaciers, landslides, and steep rock walls) at altitudes ranging from 2304 to 4003 ma.s.l. and spread across the Swiss Alps. The primary data products consist of raw GNSS observables in RINEX format, inclinometers, and weather station data. Additionally, cleaned and aggregated time series of the primary data products are provided, including daily GNSS positions derived through two independent processing tool chains. The observations documented here extend beyond the dataset presented in the paper and are currently continued with the intention of long-term monitoring. An annual update of the dataset, available at https://doi.org/10.1594/PANGAEA.948334 (Beutel et al., 2022), is planned. With its future continuation, the dataset holds potential for advancing fundamental process understanding and for the development of applied methods in support of e.g. natural hazard management
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