TEM tomography of pores with application to computational nanoscale flows in nanoporous silicon nitride (NPN)

Silicon nanomembrane technologies (NPN, pnc-Si, and others) have been used commercially as electron microscopy (EM) substrates, and as filters with nanometer-resolution size cut-offs. Combined with EM, these materials provide a platform for catching or suspending nanoscale-size structures for analysis. Usefully, the nanomembrane itself can be manufactured to achieve a variety of nanopore topographies. The size, shapes, and surfaces of nanopores will influence transport, fouling, sieving, and electrical behavior. Electron tomography (ET) techniques used to recreate nanoscale-sized structures would provide an excellent way to capture this variation. Therefore, we modified a sample holder to accept our standardized 5.4 mm × 5.4 mm silicon nanomembrane chips and imaged NPN nanomembranes (50–100 nm thick, 10–100 nm nanopore diameters) using transmission electron microscopy (TEM). After imaging and ET reconstruction using a series of freely available tools (ImageJ, TomoJ, SEG3D2, Meshlab), we used COMSOL Multiphysics™ to simulate fluid flow inside a reconstructed nanopore. The results show flow profiles with significantly more complexity than a simple cylindrical model would predict, with regions of stagnation inside the nanopores. We expect that such tomographic reconstructions of ultrathin nanopores will be valuable in elucidating the physics that underlie the many applications of silicon nanomembranes

On the physical origins of the negative index of refraction

The physical origins of negative refractive index are derived from a dilute microscopic model, producing a result that is generalized to the dense condensed phase limit. In particular, scattering from a thin sheet of electric and magnetic dipoles driven above resonance is used to form a fundamental description for negative refraction. Of practical significance, loss and dispersion are implicit in the microscopic model. While naturally occurring negative index materials are unavailable, ferromagnetic and ferroelectric materials provide device design opportunities.Comment: 4 pages, 1 figur

Randomised double blind placebo controlled trial investigating the effect of calcium and vitamin D supplementation on bone mineral density and bone metabolism in adult patients with cystic fibrosis

AbstractBackgroundLow bone mineral density (BMD) is prevalent in adults with cystic fibrosis and might be related to calcium and vitamin D malabsorption from the gastrointestinal tract. The aim of this study was to investigate the effect of calcium and vitamin D supplementation on BMD and bone metabolism in these subjects.MethodsPatients were invited to participate if they had a BMD Z score of −1 or less in the lumbar spine, proximal femur or distal forearm. Patients were randomised to receive calcium 1 g+vitamin D 800 IU or placebo daily, in addition to their regular vitamin D supplements (900 IU/day). BMD and bone biochemical markers were measured before and after 1 year of treatment.ResultsAfter 12 months, the treatment group (n=15) showed a reduced rate of bone loss compared with the control group (n=15) in the lumbar spine (mean difference 1.9% [CI −0.9% to 4.6%]), total hip (mean difference 0.7% [CI −2.2% to 3.5%]) and distal forearm (mean difference 1.7% [CI −2.2% to 5.5%]), but these changes did not reach statistical significance. There was also a trend towards a reduction in bone turnover in the treatment group.ConclusionsCalcium and vitamin D supplementation reduced the rate of bone turnover and bone loss in adult patients with cystic fibrosis, but these changes did not reach statistical significance. These data suggest that a longer term trial of this simple intervention would be justified

Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells

Although the functionality of the lens water channels aquaporin 1 (AQP1; epithelium) and AQP0 (fiber cells) is well established, less is known about the role of AQP5 in the lens. Since in other tissues AQP5 functions as a regulated water channel with a water permeability (PH2O) some 20 times higher than AQP0, AQP5 could function to modulate PH2O in lens fiber cells. To test this possibility, a fluorescence dye dilution assay was used to calculate the relative PH2O of epithelial cells and fiber membrane vesicles isolated from either the mouse or rat lens, in the absence and presence of HgCl2, an inhibitor of AQP1 and AQP5. Immunolabeling of lens sections and fiber membrane vesicles from mouse and rat lenses revealed differences in the subcellular distributions of AQP5 in the outer cortex between species, with AQP5 being predominantly membranous in the mouse but predominantly cytoplasmic in the rat. In contrast, AQP0 labeling was always membranous in both species. This species-specific heterogeneity in AQP5 membrane localization was mirrored in measurements of PH2O, with only fiber membrane vesicles isolated from the mouse lens, exhibiting a significant Hg2+-sensitive contribution to PH2O. When rat lenses were first organ cultured, immunolabeling revealed an insertion of AQP5 into cortical fiber cells, and a significant increase in Hg2+-sensitive PH2O was detected in membrane vesicles. Our results show that AQP5 forms functional water channels in the rodent lens, and they suggest that dynamic membrane insertion of AQP5 may regulate water fluxes in the lens by modulating PH2O in the outer cortex

Metal Depletion and Warm H2 in the Brown Dwarf 2M1207 Accretion Disk

We present new far-ultraviolet observations of the young M8 brown dwarf 2MASS J12073346-3932539, which is surrounded by an accretion disk. The data were obtained using the Hubble Space Telescope-Cosmic Origins Spectrograph. Moderate resolution spectra (R~17,000-18,000) obtained in the 1150-1750 A and 2770-2830 A bandpasses reveal H2 emission excited by HI Ly$\alpha$ photons, several ionization states of carbon (CI - CIV), and hot gas emission lines of HeII and NV (T ~ 10^4-5 K). Emission from some species that would be found in a typical thermal plasma at this temperature (SiII, SiIII, SiIV, and MgII) are not detected. The non-detections indicate that these refractory elements are depleted into grains, and that accretion shocks dominate the production of the hot gas observed on 2MASS J12073346-3932539. We use the observed CIV luminosity to constrain the mass accretion rate in this system. We use the kinematically broadened H2 profile to confirm that the majority of the molecular emission arises in the disk, measure the radius of the inner hole of the disk (R_{hole}~3R_{*}), and constrain the physical conditions of the warm molecular phase of the disk (T(H2)~2500-4000 K). A second, most likely unresolved H2 component is identified. This feature is either near the stellar surface in the region of the accretion shock or in a molecular outflow, although the possibility that this Jovian-like emission arises on the day-side disk of a 6 M_{J} companion (2M1207b) cannot be conclusively ruled out. In general, we find that this young brown dwarf disk system is a low-mass analog to classical T Tauri stars that are observed to produce H2 emission from a warm layer in their disks, such as the well studied TW Hya and DF Tau systems.Comment: ApJ, accepted. 12 pages, 10 figures. 3 tables

Beam-Energy-Dependent Two-Pion Interferometry and the Freeze-Out Eccentricity of Pions Measured in Heavy Ion Collisions at the STAR Detector

We present results of analyses of two-pion interferometry in Au+Au collisions at √SNN=7.7, 11.5, 19.6, 27, 39, 62.4, and 200 GeV measured in the STAR detector as part of the BNL Relativistic Heavy Ion Collider Beam Energy Scan program. The extracted correlation lengths (Hanbury-Brown–Twiss radii) are studied as a function of beam energy, azimuthal angle relative to the reaction plane, centrality, and transverse mass (mT) of the particles. The azimuthal analysis allows extraction of the eccentricity of the entire fireball at kinetic freeze-out. The energy dependence of this observable is expected to be sensitive to changes in the equation of state. A new global fit method is studied as an alternate method to directly measure the parameters in the azimuthal analysis. The eccentricity shows a monotonic decrease with beam energy that is qualitatively consistent with the trend from all model predictions and quantitatively consistent with a hadronic transport model

Strategies to optimize the impact of nutritional surveys and epidemiological studies

The development of nutrition and health guidelines and policies requires reliable scientific information. Unfortunately, theoretical considerations and empirical evidence indicate that a large percentage of science-based claims rely on studies that fail to replicate. The session "Strategies to Optimize the Impact of Nutrition Surveys and Epidemiological Studies" focused on the elements of design, interpretation, and communication of nutritional surveys and epidemiological studies to enhance and encourage the production of reliable, objective evidence for use in developing dietary guidance for the public. The speakers called for more transparency of research, raw data, consistent data-staging techniques, and improved data analysis. New approaches to collecting data are urgently needed to increase the credibility and utility of findings from nutrition epidemiological studies. Such studies are critical for furthering our knowledge and understanding of the effects of diet on health

Dielectron Azimuthal Anisotropy at Mid-Rapidity in Au + Au Collisions at √\u3cem\u3e\u3csup\u3eS\u3c/sup\u3e\u3csub\u3eNN\u3c/sub\u3e\u3c/em\u3e=200 GeV

We report on the first measurement of the azimuthal anisotropy (v2) of dielectrons (e+e− pairs) at mid-rapidity from √SNN=200 GeV Au + Au collisions with the STAR detector at the Relativistic Heavy Ion Collider (RHIC), presented as a function of transverse momentum (pT) for different invariant-mass regions. In the mass region Meec2 the dielectron v2 measurements are found to be consistent with expectations from π0, η, ω, and ϕ decay contributions. In the mass region 1.1\u3cMee\u3c2.9GeV/c2, the measured dielectron v2 is consistent, within experimental uncertainties, with that from the c¯C contributions

Surface plasmon resonance imaging of excitable cells

Surface plasmons (SPs) are surface charge density oscillations occuring at a metal/dieletric interface and are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and biological assays. In these systems, a surface plasmon resonance (SPR)-based sensor detects temporal variations in the refractive index at a point. SPR has also been used in imaging systems where the spatial variations of refractive index in the sample provide the contrast mechanism. SPR imaging systems using high numerical aperture (NA) objective lenses have been designed to image adherent live cells with high magnification and near-diffraction limited spatial resolution. Addressing research questions in cell physiology and pharmacology often requires the development of a multimodal microscope where complementary information can be obtained.In this paper, we present the development of a multimodal microscope that combines SPR imaging with a number of additional imaging modalities including bright-field, epifluorescence, total internal reflection microscopy and SPR fluorescence microscopy. We used a high NA objective lens for SPR and TIR microscopy and the platform has been used to image live cell cultures demonstrating both fluorescent and label-free techniques. Both the SPR and TIR imaging systems feature a wide field of view (~300 µm) that allows measurements from multiple cells whilst maintaining a resolution sufficient to image fine cellular processes. The capability of the platform to perform label-free functional imaging of living cells was demonstrated by imaging the spatial variations in contractions from stem cell-derived cardiomyocytes. This technique shows promise for non-invasive imaging of cultured cells over very long periods of time during development

Measurement of Longitudinal Spin Asymmetries for Weak Boson Production in Polarized Proton-Proton Collisions at RHIC

We report measurements of single- and double-spin asymmetries for W± and Z/γ∗ boson production in longitudinally polarized p+p collisions at √S=510  GeV by the STAR experiment at RHIC. The asymmetries for W± were measured as a function of the decay lepton pseudorapidity, which provides a theoretically clean probe of the proton’s polarized quark distributions at the scale of the W mass. The results are compared to theoretical predictions, constrained by polarized deep inelastic scattering measurements, and show a preference for a sizable, positive up antiquark polarization in the range 0.05\u3cx\u3c0.2