Disability Decolonized: Indigenous Peoples Enacting Self-determination

Populations researched often have little if any input in the means of data collection, analysis, or authorship of the findings published. They are excluded from participating in the scientific methods even though they are the subject of the content that is being produced. This is true for Indigenous populations and the disability community around the globe. Researchers usually use colonial methodology that does not encompass the values of these communities or have their well-being in mind. This paper examines the history of colonization and how it has infiltrated science and inhibits self-determination of Indigenous peoples. Indigenous communities need to have the means and power for self-determination. For individuals with disabilities, this includes rights to services and programs that give the respect and person-centered care they deserve to make informed decisions about their lives. Moreover, there is a recognized need for culturally appropriate services that empower American Indian and Alaska Native (AI/AN) people with disabilities to lead independent lives in their own communities—urban or rural. AI/AN cultures may view disabilities differently than those in the mainstream U.S. Barriers and challenges for AI/AN individuals with intellectual and developmental disabilities (IDD) and AI/AN families of individuals with IDD in access to services include inadequate funding, personnel shortages, housing shortages, lack of coordination among agencies, lack of consultation with tribes, and problems identifying persons eligible for services. AI/AN-specific programs that have begun to bridge the gap in access to and development of culturally competent services such as Oyáte Circle and development of collegiate courses focused on AI/AN disabilities issues. There remains a need for partnership with AI/AN tribes for disability services and incorporation of AI/AN people with disabilities as equitable partners in program development and implementation. To reach a full decolonization of IDD health care and fully embrace diversity, equity, and inclusion (DEI) principles, individuals in these communities need to be viewed as experts in their journey of resilience

Stability of the Neurotensin Receptor NTS1 Free in Detergent Solution and Immobilized to Affinity Resin

Purification of recombinant membrane receptors is commonly achieved by use of an affinity tag followed by an additional chromatography step if required. This second step may exploit specific receptor properties such as ligand binding. However, the effects of multiple purification steps on protein yield and integrity are often poorly documented. We have previously reported a robust two-step purification procedure for the recombinant rat neurotensin receptor NTS1 to give milligram quantities of functional receptor protein. First, histidine-tagged receptors are enriched by immobilized metal affinity chromatography using Ni-NTA resin. Second, remaining contaminants in the Ni-NTA column eluate are removed by use of a subsequent neurotensin column yielding pure NTS1. Whilst the neurotensin column eluate contained functional receptor protein, we observed in the neurotensin column flow-through misfolded NTS1.To investigate the origin of the misfolded receptors, we estimated the amount of functional and misfolded NTS1 at each purification step by radio-ligand binding, densitometry of Coomassie stained SDS-gels, and protein content determination. First, we observed that correctly folded NTS1 suffers damage by exposure to detergent and various buffer compositions as seen by the loss of [(3)H]neurotensin binding over time. Second, exposure to the neurotensin affinity resin generated additional misfolded receptor protein.Our data point towards two ways by which misfolded NTS1 may be generated: Damage by exposure to buffer components and by close contact of the receptor to the neurotensin affinity resin. Because NTS1 in detergent solution is stabilized by neurotensin, we speculate that the occurrence of aggregated receptor after contact with the neurotensin resin is the consequence of perturbations in the detergent belt surrounding the NTS1 transmembrane core. Both effects reduce the yield of functional receptor protein

The 2010/2011 Canterbury earthquakes context and cause of injury /

The aim of this study was to investigate causes of injury during the 2010/2011 Canterbury earthquakes. Data on patients injured during the Darfield (4 September 2010) and Christchurch (22 February 2011) earthquakes were sourced from the New Zealand Accident Compensation Corporation. The total injury burden was analyzed for demography,context of injury, causes of injury, and injury type. Injury context was classified as direct (shaking of the primary earthquake or aftershocks causing unavoidable injuries), action (movement of person during the primary earthquake or aftershocks causing potentially avoidable injuries), and secondary (cause of injury after shaking ceased). Nine categories of injury cause were identified. Three times as many people were injured in the Christchurch earthquake as in the Darfield earthquake (7,171 vs. 2,256). The primary shaking caused approximately two-thirds of the injuries from both quakes. Actions during the primary shaking and aftershocks led to many injuries (51.3 % Darfield and 19.4 % Christchurch). Primary direct caused the highest proportion of injuries during the daytime Christchurch quake (43.6 %). Many people were injured after shaking stopped in both events: 499 (22.1 % Darfield) and 1,881 (26.2 % Christchurch). Most of these people were injured during clean-up (320 (14.2 %) Darfield; 622 (8.7 %) Christchurch). In both earthquakes, more females than males (1,453 vs. 803 Darfield; 4,646 vs. 2,525 Christchurch) were injured (except by masonry, damaged ground, and during clean-up); trip/fall (27.9 % Darfield; 26.1 % Christchurch) was the most common cause of injury; and soft tissue injuries (74.1 % Darfield; 70.4 % Christchurch) was the most common type of injury. This study demonstrated that where people were and their actions during and after earthquakes influenced their risk of injury