Tub Tim Siam Rice: Applications in Cosmeceutical Products

บทคัดย่อข้าวทับทิมสยามเป็นพันธุ์ข้าวพื้นเมืองของประเทศไทย เมล็ดมีสีแดง นำข้าวที่กะเทาะเปลือกออก แล้วมาบดให้ละเอียด สกัดผงข้าวด้วยเอทานอล จากนั้นวิเคราะห์หาชนิดของสารประกอบในสารสกัดข้าวด้วยเทคนิค GC-MS และ HPLC, ปริมาณสารประกอบฟีนอลิกทั้งหมดด้วยน้ำยา Folin-Ciocalteu, ฤทธิ์ต้านออกซิเดชันด้วยเทคนิค Diphenyl-picryhydrazyl (DPPH) radical scavenging activity assay และ Ferric (Fe3+) reducing antioxidant power (FRAP) assay และคุณสมบัติช่วยสมานแผลด้วยวิธี Scratch Test พบว่าสารสกัดข้าวฯมีสารแอนโธไซยานิน กรดไขมันสายโซ่ยาว และเอสเทอร์ของกรดไขมันสายโซ่ยาวเป็นองค์ประกอบ ปริมาณสารประกอบฟีนอลิกทั้งหมดเท่ากับ 1.5 mg gallic acid equivalent/g rice powder ค่า DPPH radical scavenging activity เท่ากับ 8 mg Trolox equivalent/g rice powder และค่า FRAP activity เท่ากับ 2 mg Fe2+ equivalent/g rice powder สารสกัดข้าวที่ความเข้มข้น 25 mg/ml มีฤทธิ์ช่วยสมานแผลเมื่อทดสอบกับเซลล์ L929 ที่ได้รับบาดเจ็บ นำผงข้าวที่ผ่านการสกัดมาอบให้แห้งที่ 50 OC บรรจุลงในถุงชาขนาดเล็กแบบใช้ครั้งเดียว ของเหลวสำหรับกระจายผงข้าวเตรียมจากสารสกัดข้าว สารอีดีทีเอ กลีเซอรีนโพรพิลีนไกลคอล ไกลเด้น และพอลิไวนิลแอลกอฮอล์ เมื่อต้องการใช้ให้ผสมผงข้าว 1 ถุงกับของเหลวจนได้เพสเนื้อเนียน ทาเพสทั้งหมดลงบนของแข็งค้ำจุนผิวเรียบที่ทาน้ำมันไว้แล้ว เมื่อแห้งลอกฟิล์มที่เกิดขึ้นออกน้ำมันที่ทาลงไปตอนต้นจะถูกดึงออกระหว่างการลอกฟิล์มสามารถเพิ่มมูลค่าทางเศรษฐกิจของข้าวทับทิมสยามได้โดยผสมลงในเวชสำอางคำสำคัญ: ข้าวทับทิมสยาม สารประกอบฟีนอลิกทั้งหมด ฤทธิ์ต้านออกซิเดชัน ฤทธิ์สมานแผล มาส์คพอกหน้าแบบลอกออก ABSTRACTTub Tim Siam rice is an indigenous red rice variety of Thailand. The un-hulled rice was powdered and extracted by using ethanol. The obtained extract was investigated for phytochemicals by using GC-MS and HPLC techniques, total phenolic content (TPC) by using Folin-Ciocalteu reagent, antioxidant activity in regard to DPPH radical scavenging activity and ferric (Fe3+) reducing anti-oxidant power assays, and wound healing property according to the Scratch test. Phenolic compounds, anthocyanins, long chain fatty acids and fatty acid esters were presented in the extract. The TPC value was equal to 1.5 mg gallic acid/g of the rice powder. The DPPH radical scavenging activity and the Fe3+ reducing power were of 8 mg trolox equivalent and 2 mg Fe2+ equivalent/g, respectively. The extract exhibited wound healing property on injured L929 cells. After extraction, the rice residue was dried at 50OC and packed in single use pouches. Dispersing liquid that contains the extract, EDTA, glycerin, propylene glycol, glydant, and polyvinyl alcohol, was separately prepared and used for dispersing the rice residue in a pouch to obtain smooth paste. Transparent film was formed when left the paste to dry on solid support.Surfaced oil was effectively removed while the film was peeled off. Tub Tim Siam rice might be of economic value when incorporated in cosmeceutical products for improving skin appearance.Keywords: Tub Tim Siam rice, Total Phenolic Content, anti-oxidant activity, wound healing property, peeling face mask

The effects of Abelmoschus esculentus (L.) moench seed on high fat diet induced metabolic and cognitive impairments in C57BL/6J mice

Okra is known for its neuroprotective and antioxidant properties. We aimed to investigate the potential effects of okra seed powder in alleviating high far diet HFD-induced cognitive deficit and hypercholesterolemia. We randomly allocated thirty-six C57BL/6J male mice into: (i) control, mice fed with a normal fat level diet; (ii) HFD, mice fed with HFD; (iii) HFD-OS1; (iv) HFD-OS2; (v) HFD-OS3, mice fed with HFD and okra seed powder (200, 400, or 800 mg/kg/day, respectively); (vi) HFD-SIM, mice fed with HFD and simvastatin (20 mg/kg/day). After 10 weeks of treatment period, the mice were tested with an episodic-like memory test (EMT) and Morris water maze (MWM). We found significantly higher total and LDL cholesterol levels in mice fed with HFD. Compared to the HFD group, the control group performed better in the EMT test, and also learned and retrieved spatial reference memory better in the MWM test. The okra seed powder significantly improved spatial learning in four days of acquisition trials and the highest dose of okra profoundly improved spatial reference memory retention during the probe trial. Contrary to the MWM results, the okra-treated animals did not perform significantly better than the HFD-treated animals in EMT. At present, we recommend future studies testing the potential neuroprotective or cognitive enhancing effects of okra to assess different cognitive domains using various disease models to have a better understanding on the potential neuroprotective properties of okra

Determination of required hydrophilic-lipophilic balance of

Amesiodendron chinense (Merr.) Hu oil is reported to use for the treatment of skin disease by Thai folk medicine. Its hydrophilic-lipophilic balance (HLB) value is very important for developing a stable cream formulation. This study aimed to determine the required hydrophilic-lipophilic balance (rHLB) value of Amesiodendron chinense (Merr.) Hu oil and to develop a stable cream formulation. Herbal cream containing Amesiodendron chinense (Merr.) Hu oil was prepared by phase inversion temperature technique using water, Tween80, and Span60. The first series of 11 emulsions with HLB values ranging from 5.0 to 15.0 and the second series of eight emulsions with a smaller interval in HLB values from 5.0 to 7.8 were prepared. rHLB of coconut oil was also determined for validation of methodology. Creaming index is the established parameter to determine rHLB and to develop stable emulsion. Emulsions with optimum emulsifier concentration resulted in less percentage-creaming index. Utilizing determined rHLB, Amesiodendron chinense (Merr.) Hu cream was formulated and evaluated for different physical parameters including viscosity, color, odor, texture, and pH. Finally, the results showed the rHLB value of coconut oil and Amesiodendron chinense (Merr.) Hu oil was 5.4 and 6.2, respectively. The stable creams were developed with a 3% emulsifier. Physical parameters were found to be consistent over 6 cycles of accelerated stability test under 4 and 45○C. Physical properties of cream evaluation ensure the stability of the developed cream. rHLB value of Amesiodendron chinense (Merr.) Hu oil was exhibited, and the stable creams were successfully formulated by utilizing determined rHLB

Abelmoschus Esculentus (L.) Moench’s Peel Powder Improves High-Fat-Diet-Induced Cognitive Impairment in C57BL/6J Mice

Okra peel exhibits numerous therapeutic effects. This study explores the potential ameliorative effects of okra peel powder on high-fat-diet (HFD)-induced hypercholesterolemia and cognitive deficits. Thirty-six C57BL/6J male mice were randomly divided into six groups (n = 6 per group): (i) control, mice fed with a normal diet; (ii) HFD, mice fed with HFD; (iii) HFD-SIM, mice fed with HFD and given simvastatin (20 mg/kg/day); (iv) HFD-OP1; (v) HFD-OP2; (vi) HFD-OP3, mice fed with HFD and okra peel (200, 400, or 800 mg/kg/day, respectively). Following 10 weeks of treatments, the mice were subjected to the Morris water maze (MWM). Parameters such as weekly average body weight, food intake, and blood lipid profiles were also recorded. The HFD group showed a profound increase in total cholesterol and low-density lipoprotein concentration compared to the control group. All okra-treated and HFD-SIM groups performed better than the HFD group during acquisition trials, whereas only the HFD-OP1 produced a significantly higher number of entries into the platform zone during the probe trial. In sum, all three okra doses improved the learning ability of the mice. However, only the lowest dose of okra significantly improved the spatial reference memory retention