The conventionalisation of mock politeness in Chinese and British online forums

While much cross-cultural and cross-linguistic analysis centres on difference because it is so often what is salient in miscommunication, we argue that we need to be more aware of similarity. Drawing on corpus-assisted discourse studies, we aim to uncover similarities in the pragmatic processes across two languages/cultures, more specifically, the shared developments in the conventionalisation of apparently polite forms for impolite functions used in British and Chinese forum communities within the last decade or so. The case studies which have been selected for analysis are 'hehe' in Chinese and 'HTH' [hope that helps] in British English. In both cases, these items had previously been identified as potentially mock polite through their presence in meta-discussions of im/politeness within the forums themselves. Our analysis shows how the items become pragmaticalised within specific contexts, while remaining unaffected in others, displaying both diachronic and synchronic variation in the degree of conventionalisaton of mock politeness which they express. The differentiation between the expected behaviours in different areas of the forms (collaborative or combative) and correlation with the mock polite usage also helps explain how it is that users orient towards the conventionalised meaning even when it is still relatively low frequency compared to polite usage, i.e. low frequency but high saliency

Physiological and genetic description of dissimilatory perchlorate reduction by the novel marine bacterium Arcobacter sp. strain CAB.

A novel dissimilatory perchlorate-reducing bacterium (DPRB), Arcobacter sp. strain CAB, was isolated from a marina in Berkeley, CA. Phylogenetically, this halophile was most closely related to Arcobacter defluvii strain SW30-2 and Arcobacter ellisii. With acetate as the electron donor, strain CAB completely reduced perchlorate (ClO4(-)) or chlorate (ClO3(-)) [collectively designated (per)chlorate] to innocuous chloride (Cl(-)), likely using the perchlorate reductase (Pcr) and chlorite dismutase (Cld) enzymes. When grown with perchlorate, optimum growth was observed at 25 to 30°C, pH 7, and 3% NaCl. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) preparations were dominated by free-swimming straight rods with 1 to 2 polar flagella per cell. Strain CAB utilized a variety of organic acids, fructose, and hydrogen as electron donors coupled to (per)chlorate reduction. Further, under anoxic growth conditions strain CAB utilized the biogenic oxygen produced as a result of chlorite dismutation to oxidize catechol via the meta-cleavage pathway of aerobic catechol degradation and the catechol 2,3-dioxygenase enzyme. In addition to (per)chlorate, oxygen and nitrate were alternatively used as electron acceptors. The 3.48-Mb draft genome encoded a distinct perchlorate reduction island (PRI) containing several transposases. The genome lacks the pcrC gene, which was previously thought to be essential for (per)chlorate reduction, and appears to use an unrelated Arcobacter c-type cytochrome to perform the same function. IMPORTANCE The study of dissimilatory perchlorate-reducing bacteria (DPRB) has largely focused on freshwater, mesophilic, neutral-pH environments. This study identifies a novel marine DPRB in the genus Arcobacter that represents the first description of a DPRB associated with the Campylobacteraceae. Strain CAB is currently the only epsilonproteobacterial DPRB in pure culture. The genome of strain CAB lacks the pcrC gene found in all other DPRB tested, demonstrating a new variation on the (per)chlorate reduction pathway. The ability of strain CAB to oxidize catechol via the oxygenase-dependent meta-cleavage pathway in the absence of external oxygen by using the biogenic oxygen produced from the dismutation of chlorite provides a valuable model for understanding the anaerobic degradation of a broad diversity of xenobiotics which are recalcitrant to anaerobic metabolism but labile to oxygenase-dependent mechanisms

Extreme star formation events in quasar hosts over 0.5<z<4{\bf0.5<\textit{z}<4}

We explore the relationship between active galactic nuclei and star formation in a sample of 513 optically luminous type 1 quasars up to redshifts of $\sim$4 hosting extremely high star formation rates (SFRs). The quasars are selected to be individually detected by the \textit{Herschel} SPIRE instrument at $>$3$\sigma$ at 250 $\mu$m, leading to typical SFRs of order of 1000 M$_{\odot}$yr$^{-1}$. We find the average SFRs to increase by almost a factor 10 from $z\sim0.5$ to $z\sim3$, mirroring the rise in the comoving SFR density over the same epoch. However, we find that the SFRs remain approximately constant with increasing accretion luminosity for accretion luminosities above 10$^{12}$ L$_{\odot}$. We also find that the SFRs do not correlate with black hole mass. Both of these results are most plausibly explained by the existence of a self-regulation process by the starburst at high SFRs, which controls SFRs on time-scales comparable to or shorter than the AGN or starburst duty cycles. We additionally find that SFRs do not depend on Eddington ratio at any redshift, consistent with no relation between SFR and black hole growth rate per unit black hole mass. Finally, we find that high-ionisation broad absorption line (HiBAL) quasars have indistinguishable far-infrared properties to those of classical quasars, consistent with HiBAL quasars being normal quasars observed along a particular line of sight, with the outflows in HiBAL quasars not having any measurable effect on the star formation in their hosts.Comment: 12 pages, 6 figure

Ligation of anti-cancer drugs to self-assembling ultrashort peptides by click chemistry for localized therapy

Self-assembling ultrashort peptides from aliphatic amino acids were functionalized with platinum anti-cancer drugs by click chemistry. Oxaliplatin-derived hybrid peptide hydrogels with up to 40% drug loading were tested for localized breast cancer therapy. Stably injected gels showed significant tumor growth inhibition in mice and a better tolerance compared to the free platinum drug

The role of nanos in maternal specification of abdomen in Drosophila

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.Includes bibliographical references.by Charlotte I. Wang.Ph.D

Microvesicles and exosomes: new players in metabolic and cardiovascular disease

The past decade has witnessed an exponential increase in the number of publications referring to extracellular vesicles (EVs). For many years considered to be extracellular debris, EVs are now seen as novel mediators of endocrine signalling via cell-to-cell communication. With the capability of transferring proteins and nucleic acids from one cell to another, they have become an attractive focus of research for different pathological settings and are now regarded as both mediators and biomarkers of disease including cardio-metabolic disease. They also offer therapeutic potential as signalling agents capable of targeting tissues or cells with specific peptides or miRNAs. In this review, we focus on the role that microvesicles (MVs) and exosomes, the two most studied classes of EV, have in diabetes, cardiovascular disease, endothelial dysfunction, coagulopathies, and polycystic ovary syndrome. We also provide an overview of current developments in MV/exosome isolation techniques from plasma and other fluids, comparing different available commercial and non-commercial methods. We describe different techniques for their optical/biochemical characterization and quantitation. We also review the signalling pathways that exosomes and MVs activate in target cells and provide some insight into their use as biomarkers or potential therapeutic agents. In summary, we give an updated focus on the role that these exciting novel nanoparticles offer for the endocrine community

In vitro wound healing of tumor cells: inhibition of cell migration by selected cytotoxic alkaloids

Background: Cell migration is involved in several pathological processes such as tumor invasion, neoangiogenesis and metastasis. Microtubules are needed in directional migration. Methods: To investigate the effects of microtubule-binding agents (paclitaxel, vinblastine, colchicine, podophyllotoxin), benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelidonine) and other anti-tumor drugs (homoharringtonine, doxorubicin) on cell migration, we performed the in vitro wound healing assay. The interactions between selected alkaloids and microtubules were studied via U2OS cells expressing microtubule-GFP markers. Results: The microtubule-binding natural products paclitaxel, vinblastine, colchicine and podophyllotoxin significantly altered microtubule dynamics in living cells and inhibited cell migration at concentrations below apparent cytotoxicity. The benzophenanthridine alkaloid sanguinarine, chelerythrine and chelidonine which affected microtubules in living cells, did not inhibit cell migration. Homoharringtonine (protein biosynthesis inhibitor) and doxorubicin significantly inhibited cell migration, however, they did not exert obvious effects on microtubules. Conclusion: In this study, we demonstrated that microtubule-binding agents are effective anti-migrating agents; moreover, homoharringtonine and doxorubicin can be referred as anti-migrating agents, but direct microtubule dynamics are not involved in their mode of action. Our study provides evidence that some alkaloids and other microtubule-binding natural products may be interesting candidates for the development of novel agents against metastasis

Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-Tagged Collagen Constructs

Type I collagen is the most abundant extracellular matrix protein in bone and other connective tissues and plays key roles in normal and pathological bone formation as well as in connective tissue disorders and fibrosis. Although much is known about the collagen biosynthetic pathway and its regulatory steps, the mechanisms by which it is assembled extracellularly are less clear. We have generated GFPtpz and mCherry-tagged collagen fusion constructs for live imaging of type I collagen assembly by replacing the α2(I)-procollagen N-terminal propeptide with GFPtpz or mCherry. These novel imaging probes were stably transfected into MLO-A5 osteoblast-like cells and fibronectin-null mouse embryonic fibroblasts (FN-null-MEFs) and used for imaging type I collagen assembly dynamics and its dependence on fibronectin. Both fusion proteins co-precipitated with α1(I)-collagen and remained intracellular without ascorbate but were assembled into α1(I) collagen-containing extracellular fibrils in the presence of ascorbate. Immunogold-EM confirmed their ultrastuctural localization in banded collagen fibrils. Live cell imaging in stably transfected MLO-A5 cells revealed the highly dynamic nature of collagen assembly and showed that during assembly the fibril networks are continually stretched and contracted due to the underlying cell motion. We also observed that cell-generated forces can physically reshape the collagen fibrils. Using co-cultures of mCherry- and GFPtpz-collagen expressing cells, we show that multiple cells contribute collagen to form collagen fiber bundles. Immuno-EM further showed that individual collagen fibrils can receive contributions of collagen from more than one cell. Live cell imaging in FN-null-MEFs expressing GFPtpz-collagen showed that collagen assembly was both dependent upon and dynamically integrated with fibronectin assembly. These GFP-collagen fusion constructs provide a powerful tool for imaging collagen in living cells and have revealed novel and fundamental insights into the dynamic mechanisms for the extracellular assembly of collagen

Generalized quasi-likelihood ratio tests for varying coefficient quantile regression models

Quantile regression models which can track the relationship of predictive variables and the response variable in specific quantiles are especially useful in applications when extreme quantiles instead of the center of the distribution are interesting. Compared to classical conditional mean regressions, quantile regression models can provide a more comprehensive structure of the conditional distribution of the response variable. Also, they are more robust to skewed distributions and outliers. Therefore, quantile regression models have been applied extensively in many applied areas. Due to its greater flexibility, a varying coefficient regression technique has been extended to the quantile regression models recently. In this dissertation, my aim is to propose a new test procedure, termed as generalized quasi-likelihood (GQLR) test, to test whether all or partial coefficients are indeed constant or of some specific functions for the varying coefficient quantile regression models. The test statistics are constructed based on the comparison of the quasi-likelihood functions under null and alternative hypotheses. The asymptotic distributions of the proposed test statistics are also derived. First, the functional coefficients in a varying coefficient quantile regression model are estimated by applying local linear fitting technique with jackknife method. Then, I construct the generalized quasi-likelihood ratio test statistics to test whether the varying coefficients are of some specific functional forms, including two special cases: testing whether the varying coefficients are known or unknown constants. The asymptotic normality of the proposed test statistic is derived upon the Bahadur representation of the estimators. I also discuss how to estimate the asymptotic variance-covariance matrix and investigate the power of the proposed test procedures in Chapter 2. Secondly, I consider the similar testing procedure to test if partial coefficients in a varying coefficient quantile regression model are constant or of some specific form with other coefficients completely unspecified in Chapter 3. The corresponding generalized quasi-likelihood ratio test statistic is constructed based on comparing the quasi-likelihood functions under the null and alternative hypotheses. The asymptotic distributions of the proposed test statistics for both constancy and specific functional form are derived respectively and the power of the proposed test procedures is also investigated. Finally, to exam the finite sample performance of all test statistics proposed. In Chapters 2 and 3, Monte Carlo simulation studies are conducted respectively at the end of each chapter. I also apply the proposed test methodologies to test if the existing models in the literature used to analyze the Boston house price data are appropriate or not. The simulation results and the real example illustrate the effectiveness and practical usefulness of the proposed test statistics. Chapter 4 concludes the dissertation. I also discuss some future research topics related to this dissertation