The Effects of Anthropogenic Noise Playbacks on Marine Invertebrates

Anthropogenic sound has profoundly changed the acoustic environment of aquatic habitats, with growing evidence that even a short exposure to man-made sound sources can negatively affect marine organisms. Marine invertebrates have received little attention regarding their responses to anthropogenic sound, despite their pivotal role in marine ecosystems, and preliminary evidence of their sensitivity. In this thesis, I critically review the methods used in studies investigating the effects of anthropogenic noise on marine invertebrates. I identify methodological trends that have developed along the timeline of this topic, and use this information to suggest three research strategies to further the development of research in this field. From this review, current knowledge gaps are identified, and two main routes to address them are taken in this thesis.Firstly, to address the shortage of particle motion data in anthropogenic sound literature, two new low-cost and easily accessible particle motion sensors were developed and tested, one of them at 0.1% of the cost of currently commercially available models. These sensors will allow the measurement and reproduction of the sound fields experienced by marine invertebrates in bioacoustic research, even when research budgets are tight. Particle motion is the main sound component detected by invertebrates, yet neglected in many aquatic bioacoustical studies.Secondly, to expand on the comparably small quantity of research investigating the effects of anthropogenic sound on marine invertebrates, a series of controlled laboratory experiments were conducted. Two commercially and ecologically important model species were chosen, the blue mussel Mytilus edulis, representing sessile benthic invertebrates, and the European lobster Homarus gammarus, representing mobile benthic invertebrates.Experiments on M. edulis were conducted taking a mechanistic, integrative approach to investigate the effects of sound on multiple levels of biological organisation, including biochemistry, physiology, and behaviour. The ultimate aim was to understand the underlying drivers behind, and interactions between, responses. Comet assay analysis of haemocytes and gill cells demonstrated a significant six-fold higher single strand breakage in the DNA of cells of mussels exposed to ship-noise playback, compared to those kept under ambient conditions. Superoxide dismutase analysis did not identify an excess of superoxide ions, and glutathione, and glutathione peroxidase assays showed no increase in either glutathione or glutathione peroxidase. TBAR assays however revealed 68% more thiobarbituric acid reactive substances, indicating lipid peroxidation in the gill epithelia of noise exposed specimens. Algal clearance rates and oxygen-consumption rates of noise-exposed mussels were significantly lower (84% reduction and 12% reduction respectively), than those of control animals, while valve gape was significantly (60%) wider. This seemingly converse reaction indicates a shock response in mussels with the onset of noise exposure. Finally, at the genetic level, heat shock protein 70 expression was investigated, but no change was identified during noise exposure.Investigation into the noise induced behavioural responses of H. gammarus suggests seasonal differences in behaviour, using movement as a metric, in response to anthropogenic noise playbacks. During both summer and winter exposures, lobsters spent most time away from the highest noise area (98% of the observed time in summer and 78% in winter). The observed seasonal differences in the time spent in the highest noise area (2% in summer and 22% in winter) could be linked to the lobsters’ requirement for shelter during winter. This requirement seems to have had a stronger influence over the animals’ behaviour than any desire to avoid high noise levels.The information generated in this thesis can be used by researchers working in the field of marine sound to develop well rounded studies exploring the effects of sound on not only marine invertebrates but other faunal groups as well. The construction details provided to produce low cost particle motion sensors, will allow bioacoustic researchers to easily include particle motion measurements in all future studies investigating the effects of sound on fish and invertebrates. The results of the conducted mussel and lobster experiments evidence how multiple aspects of invertebrate biology can be affected by noise. The observed impacts on both sessile and mobile life forms highlight that the effects of noise cannot be fully understood before a wide range of species with different biological and ecological traits have been studied. The integrative approach to noise research used here can serve as a model for other species, and the results pooled to inform governments and industry of the effects of anthropogenic noise in the marine environment

From DNA to ecological performance: Effects of anthropogenic noise on a reef-building mussel

Responses of marine invertebrates to anthropogenic noise are insufficiently known, impeding our understanding of ecosystemic impacts of noise and the development of mitigation strategies. We show that the blue mussel, Mytilus edulis, is negatively affected by ship-noise playbacks across different levels of biological organization. We take a novel mechanistic multi-method approach testing and employing established ecotoxicological techniques (i.e. Comet Assay and oxidative stress tests) in combination with behavioral and physiological biomarkers. We evidence, for the first time in marine species, noise-induced changes in DNA integrity (six-fold higher DNA single strand-breaks in haemocytes and gill epithelial cells) and oxidative stress (68% increased TBARS in gill cells). We further identify physiological and behavioral changes (12% reduced oxygen consumption, 60% increase in valve gape, 84% reduced filtration rate) in noise-exposed mussels. By employing established ecotoxicological techniques we highlight impacts not only on the organismal level, but also on ecological performance. When investigating species that produce little visually obvious responses to anthropogenic noise, the above mentioned endpoints are key to revealing sublethal effects of noise and thus enable a better understanding of how this emerging, but often overlooked stressor, affects animals without complex behaviors. Our integrated approach to noise research can be used as a model for other invertebrate species and faunal groups, and inform the development of effective methods for assessing and monitoring noise impacts. Given the observed negative effects, noise should be considered a potential confounding factor in studies involving other stressors

Marine invertebrates and noise

Within the set of risk factors that compromise the conservation of marine biodiversity, one of the least understood concerns is the noise produced by human operations at sea and from land. Many aspects of how noise and other forms of energy may impact the natural balance of the oceans are still unstudied. Substantial attention has been devoted in the last decades to determine the sensitivity to noise of marine mammals—especially cetaceans and pinnipeds—and fish because they are known to possess hearing organs. Recent studies have revealed that a wide diversity of invertebrates are also sensitive to sounds, especially via sensory organs whose original function is to allow maintaining equilibrium in the water column and to sense gravity. Marine invertebrates not only represent the largest proportion of marine biomass and are indicators of ocean health but many species also have important socio-economic values. This review presents the current scientific knowledge on invertebrate bioacoustics (sound production, reception, sensitivity), as well as on how marine invertebrates are affected by anthropogenic noises. It also critically revisits the literature to identify gaps that will frame future research investigating the tolerance to noise of marine ecosystems

Noise negatively affects foraging and antipredator behaviour in shore crabs

Acoustic noise has the potential to cause stress, to distract and to mask important sounds, and thus to affect behaviour. Human activities have added considerable noise to both terrestrial and aquatic habitats, and there is growing evidence that anthropogenic noise affects communication and movement patterns in a variety of species. However, there has been relatively little work considering the effect on behaviours that are fundamental to survival, and thus have direct fitness consequences. We conducted a series of controlled tank-based experiments to consider how playback of ship noise, the most common source of underwater noise, affects foraging and antipredator behaviour in the shore crab, Carcinus maenas. Ship noise playback was more likely than ambient-noise playback to disrupt feeding, although crabs experiencing the two sound treatments did not differ in their likelihood of, or speed at, finding a food source in the first place. While crabs exposed to ship noise playback were just as likely as ambient-noise controls to detect and respond to a simulated predatory attack, they were slower to retreat to shelter. Ship noise playback also resulted in crabs that had been turned on their backs righting themselves faster than those experiencing ambient-noise playback; remaining immobile may reduce the likelihood of further predatory attention. Our findings therefore suggest that anthropogenic noise has the potential to increase the risks of starvation and predation, and showcases that the behaviour of invertebrates, and not just vertebrates, is susceptible to the impact of this pervasive global pollutant

Size-dependent physiological responses of shore crabs to single and repeated playback of ship noise

Anthropogenic noise has fundamentally changed the acoustics of terrestrial and aquatic environments, and there is growing empirical evidence that even a single noise exposure can affect behaviour in a variety of vertebrate organisms. Here, we use controlled experiments to investigate how the physiology of a marine invertebrate, the shore crab (Carcinus maenas), is affected by both single and repeated exposure to ship-noise playback. Crabs experiencing ship-noise playback consumed more oxygen, indicating a higher metabolic rate and potentially greater stress, than those exposed to ambient-noise playback. The response to single ship-noise playback was size-dependent, with heavier crabs showing a stronger response than lighter individuals. Repeated exposure to ambient-noise playback led to increased oxygen consumption (probably due to handling stress), whereas repeated exposure to ship-noise playback produced no change in physiological response; explanations include the possibility that crabs exhibited a maximal response on first exposure to ship-noise playback, or that they habituated or become tolerant to it. These results highlight that invertebrates, like vertebrates, may also be susceptible to the detrimental impacts of anthropogenic noise and demonstrate the tractability for more detailed investigations into the effects of this pervasive global pollutant

Changes in contraceptive and sexual behaviours among unmarried young people in Nigeria: Evidence from nationally representative surveys.

ContextNigeria is a high-burden country in terms of young people's health. Understanding changes in young people's sexual and reproductive health (SRH) behaviours and the associated factors is important for framing appropriate interventions.ObjectiveThis study assessed changes in SRH behaviours of unmarried young people aged 15-24 and associated factors over a ten-year period in Nigeria.Data and methodWe analysed datasets from Nigeria Demographic and Health Surveys of 2008, 2013 and 2018 to assess changes in inconsistent condom use, non-use of modern contraceptives; multiple sexual partnership; and early sexual debut. Using binary logistic regression, we assessed the association of selected variables with the SRH behaviours.ResultsOver four-fifths of unmarried young people (15-24) in Nigeria engaged in at least one risky sexual behaviour in each survey year. The pattern of changes in the four risky SRH behaviours was consistent over the 10-year period, with the highest rates of each behaviour occurring in 2018 while the lowest rates were in 2013, thus indicating an increase in the proportion of respondents engaging in risky sexual behaviours over the study period. Comprehensive HIV/AIDS knowledge, male gender, older age category (20-24), residence in south-west Nigeria, urban residence, higher socio-economic status, secondary/higher education were mostly protective against the four SRH variables analysed across the different data waves.ConclusionAddressing the high and increasing level of risky SRH behaviours among young people in Nigeria is imperative to improve overall national health status and to ensure progress towards achieving SDG target 3.7 focusing on SRH

Wale et al_data file

Analysed oxygen consumption data from playback experiments