Temperature influence on the carbon isotopic composition of Orbulina universa and Globigerina bulloides (planktonic foraminifera)

Laboratory experiments with the planktonic foraminifera Orbulina universa (symbiotic) and Globigerina bulloides (nonsymbiotic) were used to examine the effects of temperature, irradiance (symbiont photosynthesis), [CO32-], [HPO42-], and ontogeny on shell d13C values. In ambient seawater ([CO32-] = 171 mmol kg-1), the d13C of O. universa shells grown under low light (LL) levels is insensitive to temperature and records the d13C value of seawater TCO2. In contrast, the d13C of high light (HL) shells increases ~0.4‰ across 15-25°C (+0.050‰/°C). This suggests that the d13C enrichment due to symbiont photosynthetic activity is temperature-dependent. A comparison of HL O. universa grown in elevated [CO32-] seawater with ambient specimens shows that temperature does not affect the slope of the d13C/[CO32-] relationship previously described [Spero et al., 1997]. The d13C of G. bulloides shells decreases across the 15-24°C temperature range and d13C:temperature slopes decrease with increasing shell size (-0.13, -0.10, and -0.09‰/°C in 11- 12-, and 13-chambered shells, respectively). The pattern of lower d13C values at higher temperatures likely results from the incorporation of more respired CO2 into the shell at higher metabolic rates. The d13C of HL O. universa increases with increased seawater [HPO42-]

1,3,4,6-Tetramethyl-1,4-dihydro-1,2,4,5-tetrazine, C_6H_(12)N_4

M_r =140∙19, monoclinic, P2_1/n, a = 10∙612(3), b=6∙820(1), c= 10∙975 (2) Å, β=95∙31(2)°, V=790.9(5) Å^3, Z=4, D_m=1.13(5) (flotation), D_x = 1∙177 g cm^(-3), Mo Kα, λ = 0.71073 Å, μ= 0.848 cm^(-1), F(000) = 304, T= 295 K, R = 0∙077 for 704 observed reflections. This potentially antiaromatic or homoaromatic ring system has a flattened boat conformation with both N-methyls in equatorial positions. Bond angles and distances (excluding H's) predicted to be symmetry equivalent exhibit variations of 0.002-0.014 Å and 0.0-2.0°. Substantial delocalization of the electron lone pairs of N(1) and N(4) is found

ATS-6 spacecraft: In-flight antenna pattern measurement

Antenna patterns, principally associated with the 9.1 meter parabolic antenna of the ATS-6 spacecraft, were measured while in orbit at quasi-stationary synchronous altitude. Controlling the spacecraft attitude permitted a scanning of the spacecraft antenna pattern over the Rosman ground station, thus achieving the measurement of the antenna pattern contour. Patterns were determined in terms of relative gain referenced in position to the spacecraft body coordinates by means of signal power measurements made using a linear detector. These data were subsequently correlated with the attitude data to define the antenna patterns. Antenna patterns measured are presented and compared with available preflight patterns

HIV subtype and drug resistance patterns among drug naïve persons in Jos, Nigeria

To determine HIV-1 subtypes and antiretroviral drug resistance mutations for 16 infected, pregnant women in Jos, Nigeria, part of pol (1040 bp) was amplified from patient PBMC DNA, sequenced andanalyzed. Eight of the samples were subtype G, three were CRF02_AG and 2 were unique recombinant forms (URF) between G and CRF02_AG. The remaining consisted of 3 different strains: one was subtypeC, and the other 2 were unrelated URF. Nearly full-length genome sequences were completed for 6 of the strains: 4 subtype G and 2 CRF02_AG. In the 14 drug-naïve subjects, no primary resistance-associated mutations were found, but secondary mutations were identified in 7 different codons of the gene coding for protease: PR K20I, M36I, L63A/P/V, V82I, L10M/I and I93L. In addition, the K238R mutation was identified in the reverse transcriptase gene of 3 viruses. The PR K20I and M36I mutations occurred in all of the strains, and the L10M and V82I mutations occurred only in subtype G. The mutation, I93L, was carried by subtype C viruses. Two of the women that had prior niverapine treatment, had primary resistance-associated mutations, RT M184V and K103N, archived in their proviral DNA several months after treatment cessation. The study reports a predominance of clade G and CRF02_AG, and provides many more examples of nearly full-length genome sequences for subtype G viruses from Nigeria. The ubiquitous presence of PI secondary resistance-associated mutations, as well as primary resistanceassociatedmutations in 2 previously treated women, underscores the need to ensure adherence compliance to treatment

ATLASGAL: 3-mm class I methanol masers in high-mass star formation regions

We analyzed the 3-mm wavelength spectral line survey of 408 ATLASGAL clumps observed with the IRAM 30m-telescope, focusing on the class I methanol masers with frequencies near 84, 95 and 104.3 GHz. We detect narrow, maser-like features towards 54, 100 and 4 sources in the maser lines near 84, 95 and 104.3 GHz, respectively. Among them, fifty 84 GHz masers, twenty nine 95 GHz masers and four rare 104.3 GHz masers are new discoveries. The new detections increase the number of known 104.3 GHz masers from 5 to 9. The 95 GHz class I methanol maser is generally stronger than the 84 GHz maser counterpart. We find 9 sources showing class I methanol masers but no SiO emission, indicating that class I methanol masers might be the only signpost of protostellar outflow activity in extremely embedded objects at the earliest evolutionary stage. Class I methanol masers that are associated with sources that show SiO line wings are more numerous and stronger than those without such wings. The total integrated intensity of class I methanol masers is well correlated with the integrated intensity and velocity coverage of the SiO (2--1) emission. The properties of class I methanol masers are positively correlated with the bolometric luminosity, clump mass, peak H$_2$ column density of their associated clumps but uncorrelated with the luminosity-to-mass ratio, dust temperature, and mean H$_2$ volume density. We suggest that the properties of class I masers are related to shocks traced by SiO. Based on our observations, we conclude that class I methanol masers at 84 and 95 GHz can trace a similar evolutionary stage as H$_2$O maser, and appear prior to 6.7 and 12.2 GHz methanol and OH masers. Despite their small number, the 104.3 GHz class I masers appear to trace a short and more evolved stage compared to the other class I masers. [abridged]Comment: 23 pages, 27 figures, 8 tables, accepted for publication in A&

Detection of internally deposited actinides. Part IV. Preliminary considerations in the use of large, planar intrinsic Ge detectors

Many inherent advantages are apparent in the use of large planar Ge detectors for the detection, identification, and quantitative assessment of internally deposited actinide nuclides ($sup 238$Pu, $sup 239$Pu, $sup 240$Pu, $sup 242$Pu, $sup 241$Am, $sup 244$Cm, $sup 246$Cm, and $sup 250$Cf). The superior energy resolution in contrast to Phoswich type scintillation detectors, permits isotopic identifications to be made and reduces ambiguities introduced by natural and human background effects. Preliminary studies indicate that a 10 cm$sup 2$ x 1.2 cm Ge detector is comparable in detection sensitivity to that of one 125 cm$sup 2$ Phoswich detector. Some preliminary considerations and evaluations, photon transport studies based on Monte Carlo modeling and measurements of absolute L-series X-ray yields in the decay of several actinide nuclides, are presented. (auth

The structural basis for the specificity of pyridinylimidazole inhibitors of p38 MAP kinase

AbstractBackground: The p38 mitogen-activated protein (MAP) kinase regulates signal transduction in response to environmental stress. Pyridinylimidazole compounds are specific inhibitors of p38 MAP kinase that block the production of the cytokines interleukin-1 β and tumor necrosis factor α, and they are effective in animal models of arthritis, bone resorption and endotoxin shock. These compounds have been useful probes for studying the physiological functions of the p38-mediated MAP kinase pathway.Results: We report the crystal structure of a novel pyridinylimidazole compound complexed with p38 MAP kinase, and we demonstrate that this compound binds to the same site on the kinase as does ATP. Mutagenesis showed that a single residue difference between p38 MAP kinase and other MAP kinases is sufficient to confer selectivity among pyridinylimidazole compounds.Conclusions: Our results reveal how pyridinylimidazole compounds are potent and selective inhibitors of p38 MAP kinase but not other MAP kinases. It should now be possible to design other specific inhibitors of activated p38 MAP kinase using the structure of the nonphosphorylated enzyme

Fission barriers and asymmetric ground states in the relativistic mean field theory

The symmetric and asymmetric fission path for 240Pu, 232Th, and 226Ra is investigated within the relativistic mean field model. Standard parametrizations which are well fitted to nuclear ground state properties are found to deliver reasonable qualitative and quantitative features of fission, comparable to similar nonrelativstic calculations. Furthermore, stable octupole deformations in the ground states of Radium isotopes are investigated. They are found in a series of isotopes, qualitatively in agreement with nonrelativistic models. But the quantitative details differ amongst the models and between the various relativsitic parametrizations.Comment: 30 pages RevTeX, 7 tables, 12 low resolution Gif figures (high resolution PostScript versions are available at http://www.th.physik.uni-frankfurt.de/~bender/nucl_struct_publications.html or at ftp://th.physik.uni-frankfurt.de/pub/bender

Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress

Short interfering RNAs (siRNAs) target specific mRNAs for their degradation mediated by RNA-induced silencing complex (RISC). Persistent activation of siRNA-RISC frequently leads to non-targeting toxicity. However, how cells mediate this stress remains elusive. In this communication, we found that the presence of non-targeting siRNA selectively induced the expression of an endoplasmic reticulum (ER)-resident protein, non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx), but not other ER-stress proteins including GRP78, Calnexin and XBP1. Cells suffering from constant non-targeting siRNA stress grew slower and prolonged G1 phase, while NPGPx-depleted cells accumulated mature non-targeting siRNA and underwent apoptosis. Upon the stress, NPGPx covalently bound to exoribonuclease XRN2, facilitating XRN2 to remove accumulated non-targeting siRNA. These results suggest that NPGPx serves as a novel responder to non-targeting siRNA-induced stress in facilitating XRN2 to release the non-targeting siRNA accumulation