GUÍA PEDAGÓGICA

GUÍA PEDAGÓGIC

OLERICULTURA

GUÍA DE EVALUACIÓN DEL APRENDIZAJ

GUÍA DE EVALUACION DEL APRENDIZAJE

GUÍA DE EVALUACIÓN DEL APRENDIZAJ

Supracricoid partial laryngectomy with cricohyoidoepiglottopexy in patients with radiation therapy failure

BACKGROUND: To assess functional results, complications, and success of larynx preservation in patients with recurrent squamous cell carcinoma after radiotherapy. METHODS: From a database of 40 patients who underwent supracricoid partial laryngectomy (SCPL) with cricohyoidoepiglottopexy (CHEP) from June 2001 to April 2006, eight patients were treated previously with radiotherapy due to squamous cell carcinoma of the glottic region and were treated for recurrence at the site of the primary cancer. RESULTS: SCPL with CHEP was performed in six men and two women with a mean age of 67 years due to recurrence and/or persistence at a mean time of 30 months postradiotherapy (in case #8 after concomitant chemoradiotherapy). Bilateral neck dissection at levels II-V was performed in six patients. Only case #8 presented metastasis in one node. In case #5, Delphian node was positive. It was possible to preserve both arytenoids in five cases. Definitive surgical margins were negative. Complications were encountered in seven patients. Follow-up was on average 44 months (range: 20-67 months). Organ preservation in this series was 75%, and local control was 87%. Overall 5-year survival was 50%. CONCLUSIONS: In selected patient with persistence and/or recurrence after radiotherapy due to cancer of the larynx, SCPL with CHEP seems to be feasible with acceptable local control and toxicity. Complications may occur as in previously non-irradiated patients. These complications must be treated conservatively to avoid altering laryngeal function

Epigenetic profiling linked to multisystem inflammatory syndrome in children (MIS-C): A multicenter, retrospective study

BACKGROUND: Most children and adolescents infected with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain asymptomatic or develop a mild coronavirus disease 2019 (COVID-19) that usually does not require medical intervention. However, a small proportion of pediatric patients develop a severe clinical condition, multisystem inflammatory syndrome in children (MIS-C). The involvement of epigenetics in the control of the immune response and viral activity prompted us to carry out an epigenomic study to uncover target loci regulated by DNA methylation that could be altered upon the appearance of MIS-C. METHODS: Peripheral blood samples were recruited from 43 confirmed MIS-C patients. 69 non-COVID-19 pediatric samples and 15 COVID-19 pediatric samples without MIS-C were used as controls. The cases in the two groups were mixed and divided into discovery (MIS-C = 29 and non-MIS-C = 56) and validation (MIS-C = 14 and non-MIS-C = 28) cohorts, and balanced for age, gender and ethnic background. We interrogated 850,000 CpG sites of the human genome for DNA methylation variants. FINDINGS: The DNA methylation content of 33 CpG loci was linked with the presence of MIS-C. Of these sites, 18 (54.5%) were located in described genes. The top candidate gene was the immune T-cell mediator ZEB2; and others highly ranked candidates included the regulator of natural killer cell functional competence SH2D1B; VWA8, which contains a domain of the Von Willebrand factor A involved in the pediatric hemostasis disease; and human leukocyte antigen complex member HLA-DRB1; in addition to pro-inflammatory genes such as CUL2 and AIM2. The identified loci were used to construct a DNA methylation profile (EPIMISC) that was associated with MIS-C in both cohorts. The EPIMISC signature was also overrepresented in Kawasaki disease patients, a childhood pathology with a possible viral trigger, that shares many of the clinical features of MIS-C. INTERPRETATION: We have characterized DNA methylation loci that are associated with MIS-C diagnosis. The identified genes are likely contributors to the characteristic exaggerated host inflammatory response observed in these patients. The described epigenetic signature could also provide new targets for more specific therapies for the disorder.We thank the Health Department and the Centres de Recerca de Catalunya (CERCA) Programme of the Generalitat de Catalunya, the Josep Carreras Leukaemia Foundation, Fundació La Marató de TV3 and the Cellex Foundation for institutional support. We also wish to thank all the patients, family members and staff from all the units that participated in the studyPeer Reviewed"Article signat per 22 autors/es: Veronica Davalos, Carlos A. García-Prieto, Gerardo Ferrer, Sergio Aguilera-Albesa, Juan Valencia-Ramos, Agustí Rodríguez-Palmero, Montserrat Ruiz, Laura Planas-Serra, Iolanda Jordan, Iosune Alegría, Patricia Flores-Pérez, Verónica Cantarín, Victoria Fumadó, Maria Teresa Viadero, Carlos Rodrigo, Maria Méndez-Hernández, Eduardo López-Granados, Roger Colobran, Jacques G. Rivière, Pere Soler-Palacín, Aurora Pujol, Manel Esteller"Postprint (published version

Propuesta en Supply Chain Management y Logística en la empresa CYJ Catering S.A.S.

Con el paso del tiempo las empresas han ido evolucionando en sus procesos debido a las altas demandas del mercado y a las diferentes necesidades de cada cliente, esto ha llevado a un aumento de producción que a su vez requiere diferentes tipos de procesos que cada vez se hacen más grande y difícil de mantener en control, debido a la alta competencia en la que se somete cada empresa para lograr sus objetivos, es de allí donde nace la necesidad de una cadena de gestión de suministros como solución a las necesidades de las empresas de coordinar eficientemente los flujos de materiales, el personal, la información, y diferentes tipos de procesos por medio de la logística y organización, actualmente las empresas necesitan mantenerse a la vanguardia en sus productos, brindando a sus clientes calidad y cumplimiento en sus entregas y para eso es necesario las Supply Chain Management, que se encargan desde el inicio de cada proceso hasta el fin que es el consumidor. Teniendo en cuenta los diferentes conceptos, se trabajará en la empresa C&J C.ATERING, en la implementación de Supply Chain Management, en donde se le aplicaran y desarrollaran diferentes métodos en busca de información básica de la empresa con el fin de realizar la identificación de la red de proveedores y clientes con sus respectivos niveles, que permitirán reconocer las relaciones entre todas las partes que componen la red para la realización de cada proceso estratégico planteados por (GSCF), el cual se inicia trabajando con la metodología APICS-SCOR reconociendo sus seis componentes y sus diferentes formas de aplicarlo para tener un buen resultado.Over time, companies have evolved in their processes due to the high demands of the market and the different needs of each client, this has led to an increase in production that in turn requires different types of processes that are made each time. larger and more difficult to keep in control, due to the high competition in which each company is subjected to achieve its objectives, it is from there that the need for a supply management chain arises as a solution to the needs of companies to coordinate efficiently the flow of materials, personnel, information, and different types of processes through logistics and organization, currently companies need to stay at the forefront of their products, providing their customers with quality and compliance in their deliveries and for that Supply Chain Management is necessary, which is responsible from the beginning of each process to the end, which is the consumer. Taking into account the different concepts, the company C&J C.ATERING will work on the implementation of Supply Chain Management, where different methods will be applied and developed in search of basic information of the company in order to carry out the identification of the network of suppliers and customers with their respective levels, which will allow to recognize the relationships between all the parts that make up the network for the realization of each strategic process proposed by (GSCF), which begins working with the APICS-SCOR methodology, recognizing six components and their different ways of applying it to have a good result

Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer.

Pseudomonas putida is a promising bacterial chassis for metabolic engineering given its ability to metabolize a wide array of carbon sources, especially aromatic compounds derived from lignin. However, this omnivorous metabolism can also be a hindrance when it can naturally metabolize products produced from engineered pathways. Herein we show that P. putida is able to use valerolactam as a sole carbon source, as well as degrade caprolactam. Lactams represent important nylon precursors, and are produced in quantities exceeding one million tons per year (Zhang et al., 2017). To better understand this metabolism we use a combination of Random Barcode Transposon Sequencing (RB-TnSeq) and shotgun proteomics to identify the oplBA locus as the likely responsible amide hydrolase that initiates valerolactam catabolism. Deletion of the oplBA genes prevented P. putida from growing on valerolactam, prevented the degradation of valerolactam in rich media, and dramatically reduced caprolactam degradation under the same conditions. Deletion of oplBA, as well as pathways that compete for precursors L-lysine or 5-aminovalerate, increased the titer of valerolactam from undetectable after 48 h of production to ~90 mg/L. This work may serve as a template to rapidly eliminate undesirable metabolism in non-model hosts in future metabolic engineering efforts

Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer.

Pseudomonas putida is a promising bacterial chassis for metabolic engineering given its ability to metabolize a wide array of carbon sources, especially aromatic compounds derived from lignin. However, this omnivorous metabolism can also be a hindrance when it can naturally metabolize products produced from engineered pathways. Herein we show that P. putida is able to use valerolactam as a sole carbon source, as well as degrade caprolactam. Lactams represent important nylon precursors, and are produced in quantities exceeding one million tons per year (Zhang et al., 2017). To better understand this metabolism we use a combination of Random Barcode Transposon Sequencing (RB-TnSeq) and shotgun proteomics to identify the oplBA locus as the likely responsible amide hydrolase that initiates valerolactam catabolism. Deletion of the oplBA genes prevented P. putida from growing on valerolactam, prevented the degradation of valerolactam in rich media, and dramatically reduced caprolactam degradation under the same conditions. Deletion of oplBA, as well as pathways that compete for precursors L-lysine or 5-aminovalerate, increased the titer of valerolactam from undetectable after 48 h of production to ~90 mg/L. This work may serve as a template to rapidly eliminate undesirable metabolism in non-model hosts in future metabolic engineering efforts

Look-alike humans identified by facial recognition algorithms show genetic similarities

We thank François Brunelle for providing the look-alike images. We thank CERCA Programme/Generalitat de Catalunya and the Josep Carreras Foundation for institutional support. This work was funded by the governments of Catalonia (2017SGR1080) and Spain (RTI2018-094049-B-I00, SAF2014-55000, and TIN2017-90124-P) and the Cellex Foundation. M.E. conceived and designed the study; R.S.J. M.R. C.A.G.-P. M.C.d.M. D.P. S.M. V.D. P.C. M.F.-B. I.O. C.L.-F. A.N. C.F.-T. D.A. F.M.S. X.B. A.V. and M.E. analyzed multiomics and questionnaire data; R.J. and M.E. wrote the manuscript with contributions and approval from all authors. M.E. is a consultant of Ferrer International and Quimatryx. S.M. is an employee of Ferrer International. C.F.-T. is chief technical officer of Herta Security.We thank François Brunelle for providing the look-alike images. We thank CERCA Programme/Generalitat de Catalunya and the Josep Carreras Foundation for institutional support. This work was funded by the governments of Catalonia (2017SGR1080) and Spain (RTI2018-094049-B-I00, SAF2014-55000, and TIN2017-90124-P) and the Cellex Foundation.The human face is one of the most visible features of our unique identity as individuals. Interestingly, monozygotic twins share almost identical facial traits and the same DNA sequence but could exhibit differences in other biometrical parameters. The expansion of the world wide web and the possibility to exchange pictures of humans across the planet has increased the number of people identified online as virtual twins or doubles that are not family related. Herein, we have characterized in detail a set of "look-alike" humans, defined by facial recognition algorithms, for their multiomics landscape. We report that these individuals share similar genotypes and differ in their DNA methylation and microbiome landscape. These results not only provide insights about the genetics that determine our face but also might have implications for the establishment of other human anthropometric properties and even personality characteristics

Chemoinformatic-Guided Engineering of Polyketide Synthases.

Polyketide synthase (PKS) engineering is an attractive method to generate new molecules such as commodity, fine and specialty chemicals. A significant challenge is re-engineering a partially reductive PKS module to produce a saturated β-carbon through a reductive loop (RL) exchange. In this work, we sought to establish that chemoinformatics, a field traditionally used in drug discovery, offers a viable strategy for RL exchanges. We first introduced a set of donor RLs of diverse genetic origin and chemical substrates  into the first extension module of the lipomycin PKS (LipPKS1). Product titers of these engineered unimodular PKSs correlated with chemical structure similarity between the substrate of the donor RLs and recipient LipPKS1, reaching a titer of 165 mg/L of short-chain fatty acids produced by the host Streptomyces albus J1074. Expanding this method to larger intermediates that require bimodular communication, we introduced RLs of divergent chemosimilarity into LipPKS2 and determined triketide lactone production. Collectively, we observed a statistically significant correlation between atom pair chemosimilarity and production, establishing a new chemoinformatic method that may aid in the engineering of PKSs to produce desired, unnatural products