Enantiomeric Ratio Changes of Terpenes in Essential Oils from Hybrid Eucalyptus grandis × E. tereticornis and its Pure Species

Some Eucalyptus species produce oils with biological activities and the effect of their interspecifc hybridization on the enantiomeric composition of terpenes has not been reported. The enantiomeric excesses of monoterpenes in the essential oil of Eucalyptus grandis × E. tereticornis and its parental taxa were determined by gas chromatography-mass spectrometry (GC-MS), and to resolve coelutions problems by preparative high performance liquid chromatography (HPLC)and GC-MS with two columns in series. The essential oil composition of the hybrid presented qualitative and quantitative differences with the composition of its parental taxa. Great differences were found for the enantiomeric ratio in monoterpene alcohols among the three essential oils. Our results suggest that the enantiomeric analysis can be a reliable method for the study of how theinterspecifc hybridization can module the enantiomeric chemical profle in Eucalyptus essential oils. These results suggest the use of interspecifc hybridization to improve or expand the source of bioactive compounds.Fil: Naspi, Cecilia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaFil: Alvarez Costa, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaFil: Lucia, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaFil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaFil: Masuh, Hector Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentin

Muraida e seus (des)encontros com os Lusíadas

Muraida é o primeiro texto poético, com estrutura épica, escrito em Língua Portuguesa, sobre um tema relativo ao território que hoje se configura como amazônico, e através desse épico podemos vislumbrar uma das faces da empreitada colonial. O épico possui Dedicatória, Invocação, Proposição, Narração e Epílogo e é divido em seis cantos, cuja forma e intenção são similares ao modelo camoniano: as estrofes se organizam em oitava rima camoniana e os versos são decassílabos. O poema foi escrito por Henrique João Wilkens, em 1785, no quartel da Vila de Ega, atual cidade de Tefé--AM. No entanto, sua primeira edição data de 1819 pela Imprensa do Reino

Criando aberturas em regime fechado : percursos por entre o trabalho como atividade no âmbito da socioeducação

A presente dissertação tem o intuito de produzir questões clínicas do trabalho com Agentes Socioeducadores, problematizando aspectos relativos à expansão do poder de agir em meio às tensões educar/punir/vigiar que caracterizam o exercício no campo socioeducativo. As ações envolvendo o adolescente em conflito com a lei sempre esteve fortemente ligada a práticas de cunho disciplinar e, por vezes, individualizantes, questões que se produziram a partir da herança de um período onde as políticas voltadas à infância e juventude não se organizavam de maneira a privilegiar a participação social frente aos modos de pensar e tratar o jovem. Com a emergência dos movimentos sociais que consolidaram o Estatuto da Criança e do Adolescente, efeitos outros produziram-se, no sentido de que a política pública deve se fazer pública, iniciando um processo de fortalecimento de práticas que acolham a produção do comum, o que implica pensar que é na própria experiência cotidiana do labor que os trabalhadores operam a política pública (além das diretrizes), devendo construir coletivamente estratégias instituintes, mediante um processo coanalítico operado com abordagens que tomam o trabalho pela via da atividade (Clínica da Atividade e Ergologia). Tais perspectivas colocam que, pensar o trabalho, implica em gerir a distância entre o prescrito e o real, buscando analisar como os trabalhadores efetuam cotidianamente as microgestões dos processos laborais mediante imprevisibilidades e os modos como dão sentido e experimentam o trabalho, produzindo transformações através de relações entre si e com o mundo. A atividade afirma um modo de vida não se limitando a execução de normas, pois, viver é poder criar novas normas, processo que só é possível mediante uma gestão coletiva fabricada na experiência do próprio trabalho, visando a expansão do poder de agir (questão crucial para a saúde). Os procedimentos metodológicos se deram através de um processo de pesquisa-intervenção cartográfica, onde pesquisadora e pesquisados colocaram-se como coanalistas das situações laborais. Os dispositivos empregados enquanto método de pesquisa foram o diário de campo, onde se encontra muito da voz da pesquisadora e dos movimentos do pesquisar, observações, análise de documentos, acompanhamento das situações concretas de trabalho, organização de espaços coletivos de discussão e análise da atividade no âmbito socioeducativo e Instrução ao Sósia. As análises produzidas permitiram explorar, por entre a gestão cotidiana dos processos de trabalho, peculiaridades da dinâmica expansão-constrangimento do poder de agir no e pelo trabalho Socioeducativo, discutindo que cuidar da saúde dos agentes socioeducadores pelo cuidado do ofício, abre perspectivas para a instauração de relação éticas por entre a expansão do poder de agir no trabalho da socioeducação.The present dissertation aims to produce clinical questions of the work with Socioeducational Agents acting in closed socio-educational measures, problematizing aspects related to the expansion of the power to act amid the tensions educating / punishing / that characterize the exercise in the socio-educational field. Actions involving adolescents in conflict with the law have always been strongly linked to disciplinary and, sometimes, individualizing practices, issues that have arisen from the legacy of a period when policies aimed at children and youth were not organized in a way to privilege social participation in the face of ways of thinking and treating young people. With the emergence of the social movements that consolidated the Child and Adolescent Statute, other effects took place, in the sense that public policy must be made public, initiating a process of strengthening practices that welcome the production of the common, which implies thinking that it is in own daily experience of work that workers operate public policy (in addition to the guidelines), and they must collectively build instituting strategies, through a coanalytical process that operates with approaches that take the work through the activity (Clinic of Activity and Ergology). Such perspectives suggest that thinking about work implies managing the distance between what is prescribed and what is real, seeking to analyze how workers perform micro-management of work processes on a daily basis through unpredictability and the ways in which they give meaning and experience work, producing transformations through relationships with each other and with the world. The activity affirms a way of life not limited to the execution of norms, because to live is to be able to create new norms, a process that is only possible through a collective management fabricated from the experience of the work itself, aiming at the expansion of the power to act (crucial question to health). The methodological procedures were carried out through a process of cartographic research-intervention, where the researcher and those being researched placed themselves as co-analysts of work situations. The devices used as a research method were the field diary, where much of the researcher's voice and research movements are found, observations, document analysis, monitoring of concrete work situations and organization of collective spaces for discussion and analysis of activity in the socio-educational context, in which the Instruction to the Double was also practiced. The analyzes produced made it possible to explore, among the day-to-day management of work processes, peculiarities of the dynamic expansion-constraint of the power to act in and through Socio-educational work, arguing that taking care of the health of socio-educational agents through the care of the work, opens perspectives for the establishment of ethical relations between the expansion of the power to act in the work of socioeducation

Leveraging Previous Facial Action Units Knowledge for Emotion Recognition on Faces

People naturally understand emotions, thus permitting a machine to do the same could open new paths for human-computer interaction. Facial expressions can be very useful for emotion recognition techniques, as these are the biggest transmitters of non-verbal cues capable of being correlated with emotions. Several techniques are based on Convolutional Neural Networks (CNNs) to extract information in a machine learning process. However, simple CNNs are not always sufficient to locate points of interest on the face that can be correlated with emotions. In this work, we intend to expand the capacity of emotion recognition techniques by proposing the usage of Facial Action Units (AUs) recognition techniques to recognize emotions. This recognition will be based on the Facial Action Coding System (FACS) and computed by a machine learning system. In particular, our method expands over EmotiRAM, an approach for multi-cue emotion recognition, in which we improve over their facial encoding module

High-Level Synthesis Hardware Design for FPGA-Based Accelerators: Models, Methodologies, and Frameworks

Hardware accelerators based on field programmable gate array (FPGA) and system on chip (SoC) devices have gained attention in recent years. One of the main reasons is that these devices contain reconfigurable logic, which makes them feasible for boosting the performance of applications. High-level synthesis (HLS) tools facilitate the creation of FPGA code from a high level of abstraction using different directives to obtain an optimized hardware design based on performance metrics. However, the complexity of the design space depends on different factors such as the number of directives used in the source code, the available resources in the device, and the clock frequency. Design space exploration (DSE) techniques comprise the evaluation of multiple implementations with different combinations of directives to obtain a design with a good compromise between different metrics. This paper presents a survey of models, methodologies, and frameworks proposed for metric estimation, FPGA-based DSE, and power consumption estimation on FPGA/SoC. The main features, limitations, and trade-offs of these approaches are described. We also present the integration of existing models and frameworks in diverse research areas and identify the different challenges to be addressed

Use of Thermography Techniques in Equines: Principles and Applications

none7noThis review of the use of thermographic technique in equines introduces the principles upon which infrared radiation and thermoregulatory physiology are based and describes the instrumentation used and its practical use. The advantage of this imaging technique is that it is a noninvasive thermographic examination, both from an operational (the animal and the operator) and health (no penetrating radiation is used) standpoint. Advantages and disadvantages of this technique, equine applications, and physiological assessments are discussed.openVeronica, Redaelli; Domenico, Bergero; Enrica, Zucca; Francesco, Ferrucci; Leonardo Nanni Costa, ; Lorenzo, Crosta; Fabio, LuziVeronica, Redaelli; Domenico, Bergero; Enrica, Zucca; Francesco, Ferrucci; Leonardo Nanni Costa, ; Lorenzo, Crosta; Fabio, Luz

Estimation of age at death based on the analysis of third molar mineralization in individuals from Brazilian archaeological populations

Estimating the age at death of archaeological individuals is critical for the reconstruction of the demographic profile of past populations. Teeth are very resistant to mechanical, chemical and physical damage. Thus, dental age estimation methods have been proven remarkably useful, especially when the other available bone remains are poorly preserved. In this study, we estimated the age of subadult individuals from pre-colonial archaeological sites in the state of São Paulo, Brazil, which are under the curation of the Museum of Archaeology and Ethnology at the University of São Paulo (MAE-USP). Age estimation was based on the analysis of dental mineralization stages originally proposed by Demirjian et al. (7) and applied to third molars by Soares et al. (11). Teeth (n = 18) were radiographed on a portable X-ray device and the images were analyzed in DICOM extension. The intraexaminer reliability test showed excellent agreement regarding the classification of mineralization stages (Kappa value = 0.94). This age estimation method showed good agreement with the previously cataloged age estimates, which were used for comparison. Of the 18 teeth examined, 15 had their age correctly estimated within the comparative range and only three did not match, of which two were very close to the cataloged estimates and one was underestimated by several years. Collectively, our findings suggest this method can be accurately applied to archaeological individuals

Serial interactome capture of the human cell nucleus

Novel RNA-guided cellular functions are paralleled by an increasing number of RNA-binding proteins (RBPs). Here we present ‘serial RNA interactome capture’ (serIC), a multiple purification procedure of ultraviolet-crosslinked poly(A)–RNA–protein complexes that enables global RBP detection with high specificity. We apply serIC to the nuclei of proliferating K562 cells to obtain the first human nuclear RNA interactome. The domain composition of the 382 identified nuclear RBPs markedly differs from previous IC experiments, including few factors without known RNA-binding domains that are in good agreement with computationally predicted RNA binding. serIC extends the number of DNA–RNA-binding proteins (DRBPs), and reveals a network of RBPs involved in p53 signalling and double-strand break repair. serIC is an effective tool to couple global RBP capture with additional selection or labelling steps for specific detection of highly purified RBPs

Relationship between early childhood non-parental childcare and diet, physical activity, sedentary behaviour, and sleep: a systematic review of longitudinal studies

The rising prevalence of childhood obesity is a global public health concern. Evidence suggests that exposure to non-parental childcare before age six years is associated with development of obesity, diet, and activity behaviours (physical activity, sedentary behaviour, and sleep). However, findings are inconsistent and mostly from cross-sectional studies, making it difficult to identify the direction of causation in associations. This review identified and synthesised the published research on longitudinal associations between non-parental childcare during early childhood, diet, and activity behaviours. Seven databases were searched, and results were independently double-screened through title/abstract and full-text stages. Included studies were evaluated for risk of bias. Of the 18,793 references screened, 13 met eligibility criteria and were included in the review. These presented results on 89 tested childcare/outcome associations, 63 testing diet outcomes (59% null, remainder mixed), and 26 testing activity behaviour outcomes (85% null, remainder mixed). The scarce available literature indicates little and mixed evidence of a longitudinal association. This reflects a paucity of research, rather than clear evidence of no effect. There is an urgent need for studies investigating the longitudinal associations of non-parental childcare on diet and activity behaviours to assess potential lasting effects and mechanisms; whether and how effects vary by provider; and differences by intensity, duration, and population sub-groups