El juego , estrategia pedag?gica para erradicar la agresividad y formar en la sana convivencia en los ni?os y ni?as de grado 1? de b?sica primaria

185 p. Recurso Electr?nicoEste proyecto de investigaci?n, se realiz? con la intenci?n de erradicar los comportamientos agresivos de los ni?os y ni?as del grado primero A del INSTITUTO KENNEDY, de la ciudad de Pereira, por medio del juego como herramienta pedag?gica, en busca de una sana convivencia; lo anterior en el marco del proyecto pedag?gico de aula ?Reconci-jugando?. Dicho proyecto fue realizado como trabajo de grado en la Universidad del Tolima sede Pereira, en el programa de Licenciatura en Pedagog?a Infantil. Este se enfoc? hacia una metodolog?a de investigaci?n de acci?n participaci?n; con tres momentos esenciales para su desarrollo; momento 1 caracterizaci?n, momento 2 dise?o del PPA, momento 3 intervenci?n. El PPA giro alrededor de una actividad integradora con tres n?cleos de intervenci?n: arte y juego, literatura y exploraci?n del medio, en pro de potencializar habilidades de socializaci?n y trabajo en equipo, teniendo en cuenta competencias y dimensiones del desarrollo integral de todos los ni?os. Es as?, como esta investigaci?n contribuye hacia la construcci?n de una sana convivencia de la comunidad educativa, con el fin de lograr un ambiente tolerante y libre de violencia, para que cada uno de sus miembros pueda desarrollar plenamente su personalidad, ejercer sus derechos y cumplir sus deberes correlativos, en pro de la construcci?n de paz. Palabras Claves: juego, sana convivencia, comportamientos agresivos, desarrollo integral, proyecto pedag?gico de aula (PPA).This research project was carried out with the intention of eradicating the aggressive behaviors of KENNEDY INSTITUTE first grade children, in the city of Pereira, using the game as a pedagogical tool, looking for a healthy coexistence; The previous one in the frame of the pedagogical project of classroom "Reconci-jugando". This project was made as a degree work for the University of Tolima in Pereira, in the bachelor?s degree program of children pedagogy. This project focused on a participatory action research methodology; With three essential moments for its development; Moment 1 characterization, moment 2 PPA design, moment 3 intervention. The PPA revolves around an integrative activity with three intervention nucleus: art and game, literature and exploration of the environment, in order to enhance socialization skills and teamwork, taking into account the competencies and dimensions of the integral development of all children. That is how this research contributes to the construction of a healthy coexistence of the educational community, in order to achieve a tolerant and violence-free environment, so that each of its members can fully develop their personality, practice their rights and fulfill Their correlative duties, with the aim of constructing peace. Keywords: game, healthy coexistence, aggressive behaviors, integral development, classroom pedagogical project (PPA)

beta-decay study of Cu-77

A beta-decay study of Cu-77 has been performed at the ISOLDE mass separator with the aim to deduce its beta-decay properties and to obtain spectroscopic information on Zn-77. Neutron-rich copper isotopes were produced by means of proton- or neutron-induced fission reactions on U-238. After the production, Cu-77 was selectively laser ionized, mass separated and sent to different detection systems where beta-gamma and beta-n coincidence data were collected. We report on the deduced half-live, decay scheme, and possible spin assignment of 77Cu

Teaching open and reproducible scholarship: a critical review of the evidence base for current pedagogical methods and their outcomes

In recent years, the scientific community has called for improvements in the credibility, robustness and reproducibility of research, characterized by increased interest and promotion of open and transparent research practices. While progress has been positive, there is a lack of consideration about how this approach can be embedded into undergraduate and postgraduate research training. Specifically, a critical overview of the literature which investigates how integrating open and reproducible science may influence student outcomes is needed. In this paper, we provide the first critical review of literature surrounding the integration of open and reproducible scholarship into teaching and learning and its associated outcomes in students. Our review highlighted how embedding open and reproducible scholarship appears to be associated with (i) students' scientific literacies (i.e. students’ understanding of open research, consumption of science and the development of transferable skills); (ii) student engagement (i.e. motivation and engagement with learning, collaboration and engagement in open research) and (iii) students' attitudes towards science (i.e. trust in science and confidence in research findings). However, our review also identified a need for more robust and rigorous methods within pedagogical research, including more interventional and experimental evaluations of teaching practice. We discuss implications for teaching and learning scholarship.Social decision makin

Pharmacological levels of withaferin A (Withania somnifera) trigger clinically relevant anticancer effects specific to triple negative breast cancer cells

Withaferin A (WA) isolated from Withania somnifera (Ashwagandha) has recently become an attractive phytochemical under investigation in various preclinical studies for treatment of different cancer types. In the present study, a comparative pathway-based transcriptome analysis was applied in epithelial-like MCF-7 and triple negative mesenchymal MDA-MB-231 breast cancer cells exposed to different concentrations of WA which can be detected systemically in in vivo experiments. Whereas WA treatment demonstrated attenuation of multiple cancer hallmarks, the withanolide analogue Withanone (WN) did not exert any of the described effects at comparable concentrations. Pathway enrichment analysis revealed that WA targets specific cancer processes related to cell death, cell cycle and proliferation, which could be functionally validated by flow cytometry and real-time cell proliferation assays. WA also strongly decreased MDA-MB-231 invasion as determined by single-cell collagen invasion assay. This was further supported by decreased gene expression of extracellular matrix-degrading proteases (uPA, PLAT, ADAM8), cell adhesion molecules (integrins, laminins), pro-inflammatory mediators of the metastasis-promoting tumor microenvironment (TNFSF12, IL6, ANGPTL2, CSF1R) and concomitant increased expression of the validated breast cancer metastasis suppressor gene (BRMS1). In line with the transcriptional changes, nanomolar concentrations of WA significantly decreased protein levels and corresponding activity of uPA in MDA-MB-231 cell supernatant, further supporting its anti-metastatic properties. Finally, hierarchical clustering analysis of 84 chromatin writer-reader-eraser enzymes revealed that WA treatment of invasive mesenchymal MDA-MB-231 cells reprogrammed their transcription levels more similarly towards the pattern observed in non-invasive MCF-7 cells. In conclusion, taking into account that sub-cytotoxic concentrations of WA target multiple metastatic effectors in therapy-resistant triple negative breast cancer, WA-based therapeutic strategies targeting the uPA pathway hold promise for further (pre)clinical development to defeat aggressive metastatic breast cancer

La satisfacci?n laboral y su correlaci?n con la intenci?n de rotaci?n de asesores comerciales de las instituciones microfinancieras

Desde la d?cada del 2000 se ha fortalecido el sistema microfinanciero en el Per?, generando la atenci?n de diversos organismos nacionales e internacionales; esto, debido a la contribuci?n social y la econom?a que genera a cada pa?s, por ser fuente de financiamiento para los emprendedores de escasos recursos econ?micos (SBS, 2017). Uno de los principales problemas a los que se enfrenta este sector es la rotaci?n de los asesores comerciales, este sector registra una tasa de rotaci?n de 24,2% (Asomif, 2018), superior a la tasa de rotaci?n de Latinoam?rica. En este contexto surge la necesidad de entender los motivos por los cuales los asesores comerciales tienen la intenci?n de abandonar la organizaci?n. Esta investigaci?n se fundamenta en la teor?a de Herzberg, la cual establece dos factores que influyen en la satisfacci?n laboral de los empleados: los factores extr?nsecos o de higiene, que est?n asociados al contexto en el cual trabaja el empleado, y los factores intr?nsecos o de motivaci?n, los cuales est?n asociados al contexto al contenido del trabajo. Basados en esta teor?a, se examin? la correlaci?n entre la satisfacci?n laboral de los factores extr?nsecos e intr?nsecos de los asesores comerciales, en las instituciones microfinancieras, y la intenci?n de rotaci?n. Para medir la satisfacci?n laboral se utiliz? el cuestionario de MSQ, de la Universidad de Minnesota, y la encuesta de intenci?n de rotaci?n de Boshoff & Allen. Ambos instrumentos fueron aplicados a 384 asesores comerciales usando el modelo de correlaci?n Rho de Spearman. Los resultados arrojaron que, tanto factores intr?nsecos como los extr?nsecos presentan una relaci?n inversa altamente significativa (p < 0.01); para los factores intr?nsecos la relaci?n result? -0.656 y los extr?nsecos -0,643, validando la hip?tesis propuesta en la presente investigaci?n

Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD

Extensins are plant cell wall glycoproteins that act as scaffolds for the deposition of the main wall carbohydrate polymers, which are interlocked into the supramolecular wall structure through intra- and inter-molecular iso-di-tyrosine crosslinks within the extensin backbone. In the conserved canonical extensin repeat, Ser-Hyp(4), serine and the consecutive C4-hydroxyprolines (Hyps) are substituted with an α-galactose and 1–5 β- or α-linked arabinofuranoses (Arafs), respectively. These modifications are required for correct extended structure and function of the extensin network. Here, we identified a single Arabidopsis thaliana gene, At3g57630, in clade E of the inverting Glycosyltransferase family GT47 as a candidate for the transfer of Araf to Hyp-arabinofuranotriose (Hyp-β1,4Araf-β1,2Araf-β1,2Araf) side chains in an α-linkage, to yield Hyp-Araf(4) which is exclusively found in extensins. T-DNA knock-out mutants of At3g57630 showed a truncated root hair phenotype, as seen for mutants of all hitherto characterized extensin glycosylation enzymes; both root hair and glycan phenotypes were restored upon reintroduction of At3g57630. At3g57630 was named Extensin Arabinose Deficient transferase, ExAD, accordingly. The occurrence of ExAD orthologs within the Viridiplantae along with its’ product, Hyp-Araf(4), point to ExAD being an evolutionary hallmark of terrestrial plants and charophyte green algae

A new multicompartmental reaction-diffusion modeling method links transient membrane attachment of E. coli MinE to E-ring formation

Many important cellular processes are regulated by reaction-diffusion (RD) of molecules that takes place both in the cytoplasm and on the membrane. To model and analyze such multicompartmental processes, we developed a lattice-based Monte Carlo method, Spatiocyte that supports RD in volume and surface compartments at single molecule resolution. Stochasticity in RD and the excluded volume effect brought by intracellular molecular crowding, both of which can significantly affect RD and thus, cellular processes, are also supported. We verified the method by comparing simulation results of diffusion, irreversible and reversible reactions with the predicted analytical and best available numerical solutions. Moreover, to directly compare the localization patterns of molecules in fluorescence microscopy images with simulation, we devised a visualization method that mimics the microphotography process by showing the trajectory of simulated molecules averaged according to the camera exposure time. In the rod-shaped bacterium _Escherichia coli_, the division site is suppressed at the cell poles by periodic pole-to-pole oscillations of the Min proteins (MinC, MinD and MinE) arising from carefully orchestrated RD in both cytoplasm and membrane compartments. Using Spatiocyte we could model and reproduce the _in vivo_ MinDE localization dynamics by accounting for the established properties of MinE. Our results suggest that the MinE ring, which is essential in preventing polar septation, is largely composed of MinE that is transiently attached to the membrane independently after recruited by MinD. Overall, Spatiocyte allows simulation and visualization of complex spatial and reaction-diffusion mediated cellular processes in volumes and surfaces. As we showed, it can potentially provide mechanistic insights otherwise difficult to obtain experimentally

Complexity of equational theory of relational algebras with standard projection elements

The class $\mathsf{TPA}$ of t rue p airing a lgebras is defined to be the class of relation algebras expanded with concrete set theoretical projection functions. The main results of the present paper is that neither the equational theory of $\mathsf{TPA}$ nor the first order theory of $\mathsf{TPA}$ are decidable. Moreover, we show that the set of all equations valid in $\mathsf{TPA}$ is exactly on the $\Pi ^1_1$ level. We consider the class $\mathsf{TPA}^-$ of the relation algebra reducts of $\mathsf{TPA}$’s, as well. We prove that the equational theory of $\mathsf{TPA}^-$ is much simpler, namely, it is recursively enumerable. We also give motivation for our results and some connections to related work

Continuous selections of multivalued mappings

This survey covers in our opinion the most important results in the theory of continuous selections of multivalued mappings (approximately) from 2002 through 2012. It extends and continues our previous such survey which appeared in Recent Progress in General Topology, II, which was published in 2002. In comparison, our present survey considers more restricted and specific areas of mathematics. Note that we do not consider the theory of selectors (i.e. continuous choices of elements from subsets of topological spaces) since this topics is covered by another survey in this volume