The \u3ci\u3ePhycodnaviridae\u3c/i\u3e: The Story of How Tiny Giants Rule the World

The family Phycodnaviridae encompasses a diverse and rapidly expanding collection of large icosahedral, dsDNA viruses that infect algae. These lytic and lysogenic viruses have genomes ranging from 160 to 560 kb. The family consists of six genera based initially on host range and supported by sequence comparisons. The family is monophyletic with branches for each genus, but the phycodnaviruses have evolutionary roots that connect them with several other families of large DNA viruses, referred to as the nucleocytoplasmic large DNA viruses (NCLDV).The phycodnaviruses have diverse genome structures, some with large regions of noncoding sequence and others with regions of ssDNA. The genomes of members in three genera in the Phycodnaviridae have been sequenced. The genome analyses have revealed more than 1000 unique genes, with only 14 homologous genes in common among the three genera of phycodnaviruses sequenced to date. Thus, their gene diversity far exceeds the number of so-called core genes. Not much is known about the replication of these viruses, but the consequences of these infections on phytoplankton have global affects, including influencing geochemical cycling and weather patterns

The \u3ci\u3ePhycodnaviridae\u3c/i\u3e: The Story of How Tiny Giants Rule the World

The family Phycodnaviridae encompasses a diverse and rapidly expanding collection of large icosahedral, dsDNA viruses that infect algae. These lytic and lysogenic viruses have genomes ranging from 160 to 560 kb. The family consists of six genera based initially on host range and supported by sequence comparisons. The family is monophyletic with branches for each genus, but the phycodnaviruses have evolutionary roots that connect them with several other families of large DNA viruses, referred to as the nucleocytoplasmic large DNA viruses (NCLDV).The phycodnaviruses have diverse genome structures, some with large regions of noncoding sequence and others with regions of ssDNA. The genomes of members in three genera in the Phycodnaviridae have been sequenced. The genome analyses have revealed more than 1000 unique genes, with only 14 homologous genes in common among the three genera of phycodnaviruses sequenced to date. Thus, their gene diversity far exceeds the number of so-called core genes. Not much is known about the replication of these viruses, but the consequences of these infections on phytoplankton have global affects, including influencing geochemical cycling and weather patterns

Design of a ring resonator-based optical beam forming network for phased array receive antennas

A novel squint-free ring resonator-based optical beam forming network (OBFN) for phased array antennas (PAA) is proposed. It is intended to provide broadband connectivity to airborne platforms via geostationary satellites. In this paper, we present the design of the OBFN and its control system. Our goal is to deliver large bandwidth Ku-band connectivity between antennas, mount conformal to the airplane fuselage and on a geostationary satellite, respectively.This way it would be possible to bring live DVB-S television to airplane passengers. In this paper, we present recent research conducted on a 4 × 1 ring resonator-based OBFN test set-up. This OBFN has four optical input ports and one optical output port. It is tuned to provide the desired signal combination with optimal constructive interference between the modulated input signals from the PAA. Therefore, combining circuitry and delay elements are required. The OBFN is tuned by electrically heating tunable true time delay (TTD) elements. These are built using optical ring resonators (ORRs). By cascading multiple ORRs with different resonance frequencies, it is possible to create a TTD with a large bandwidth. Optical beam forming is used because it provides advantages over traditional beam forming methods. These advantages are: large bandwidth, EMI resistance, and, when integrated onto a single chip, compactness and low costs. The OBFN is created using planar optical waveguide technology and consists of the following building blocks: waveguides, Mach-Zehnder interferometers, (MZIs) couplers and ORRs. The tuning of the OBFN is done by an electronic control system using a microcontroller. Communication with a PC is possible using USB. To our knowledge, this is the first integrated ORR-based OBFN circuit for PAA satellite reception

What are the prospects for citizen science in agriculture? Evidence from three continents on motivation and mobile telephone use of resource-poor farmers

As the sustainability of agricultural citizen science projects depends on volunteer farmers who contribute their time, energy and skills, understanding their motivation is important to attract and retain participants in citizen science projects. The objectives of this study were to assess 1) farmers’ motivations to participate as citizen scientists and 2) farmers’ mobile telephone usage. Building on motivational factors identified from previous citizen science studies, a questionnaire based methodology was developed which allowed the analysis of motivational factors and their relation to farmers’ characteristics. The questionnaire was applied in three communities of farmers, in countries from different continents, participating as citizen scientists. We used statistical tests to compare motivational factors within and among the three countries. In addition, the relations between motivational factors and farmers characteristics were assessed. Lastly, Principal Component Analysis (PCA) was used to group farmers based on their motivations. Although there was an overlap between the types of motivations, for Indian farmers a collectivistic type of motivation (i.e., contribute to scientific research) was more important than egoistic and altruistic motivations. For Ethiopian and Honduran farmers an egoistic intrinsic type of motivation (i.e., interest in sharing information) was most important. While fun has appeared to be an important egoistic intrinsic factor to participate in other citizen science projects, the smallholder farmers involved in this research valued ‘passing free time’ the lowest. Two major groups of farmers were distinguished: one motivated by sharing information (egoistic intrinsic), helping (altruism) and contribute to scientific research (collectivistic) and one motivated by egoistic extrinsic factors (expectation, expert interaction and community interaction). Country and education level were the two most important farmers’ characteristics that explain around 20% of the variation in farmers motivations. For educated farmers, contributing to scientific research was a more important motivation to participate as citizen scientists compared to less educated farmers. We conclude that motivations to participate in citizen science are different for smallholders in agriculture compared to other sectors. Citizen science does have high potential, but easy to use mechanisms are needed. Moreover, gamification may increase the egoistic intrinsic motivation of farmers

Genetic and ecological consequences of recent habitat fragmentation in a narrow endemic plant species within an urban context

Understanding the timescales that shape spatial genetic structure is pivotal to ascertain the impact of habitat fragmentation on the genetic diversity and reproductive viability of long-lived plant populations. Combining genetic and ecological information with current and past fragmentation conditions allows the identification of the main drivers important in shaping population structure and declines in reproduction, which is crucial for informing conservation strategies. Using historic aerial photographs, we defined the past fragmentation conditions for the shrub Conospermum undulatum, a species now completely embedded in an urban area. We explored the impact of current and past conditions on its genetic layout and assessed the effects of genetic and environmental factors on its reproduction. The historically high structural connectivity was evident in the genetics of the species. Despite the current intense fragmentation, we found similar levels of genetic diversity across populations and a weak spatial genetic structure. Historical connectivity was negatively associated with genetic differentiation among populations and positively related to within-population genetic diversity. Variation partitioning of reproductive performance explained ~ 66% of the variance, showing significant influences for genetic (9%), environmental (15%), and combined (42%) fractions. Our study highlights the importance of considering the historical habitat dynamics when investigating fragmentation consequences in long-lived plants. A detailed characterization of fragmentation from 1953 has shown how low levels of genetic fixation are due to extensive gene flow through the non-fragmented landscape. Moreover, knowledge of the relationships between genetic and environmental variation and reproduction can help to implement effective conservation strategies, particularly in highly dynamic landscapes

Nitric oxide synthase inhibition results in synergistic anti-tumour activity with melphalan and tumour necrosis factor alpha-based isolated limb perfusions

Nitric oxide (NO) is an important molecule in regulating tumour blood flow and stimulating tumour angiogenesis. Inhibition of NO synthase by L-NAME might induce an anti-tumour effect by limiting nutrients and oxygen to reach tumour tissue or affecting vascular growth. The anti-tumour effect of L-NAME after systemic administration was studied in a renal subcapsular CC531 adenocarcinoma model in rats. Moreover, regional administration of L-NAME, in combination with TNF and melphalan, was studied in an isolated limb perfusion (ILP) model using BN175 soft-tissue sarcomas. Systemic treatment with L-NAME inhibited growth of adenocarcinoma significantly but was accompanied by impaired renal function. In ILP, reduced tumour growth was observed when L-NAME was used alone. In combination with TNF or melphalan, L-NAME increased response rates significantly compared to perfusions without L-NAME (0–64% and 0–63% respectively). An additional anti-tumour effect was demonstrated when L-NAME was added to the synergistic combination of melphalan and TNF (responses increased from 70 to 100%). Inhibition of NO synthase reduces tumour growth both after systemic and regional (ILP) treatment. A synergistic anti-tumour effect of L-NAME is observed in combination with melphalan and/or TNF using ILP. These results indicate a possible role of L-NAME for the treatment of solid tumours in a systemic or regional setting. © 2000 Cancer Research Campaig

Experiences and Drivers of Food Insecurity in Guatemala's Dry Corridor: Insights From the Integration of Ethnographic and Household Survey Data

Eradicating hunger is a complex and multifaceted challenge, requiring evidence bases that can inform wide scale action, but that are also participatory and grounded to have local relevance and effectiveness. The Rural Household Multi-Indicator Surveys (RHoMIS) provides a broad assessment of household capabilities and food security outcomes, while ethnographic approaches evidence how individuals' perceptions, experiences and local socio-political context shape food security experiences and intervention outcomes. However, integrating these research approaches presents methodological and ontological challenges. We combine a quantitative approach with life history interviews to understand the drivers, experiences and outcomes of food insecurity in Guatemala's dry corridor region. We also reflect on the effectiveness and challenges of integrating the two methods for purposes of selective sampling, triangulating evidence, and producing a cohesive analyses of food insecurity in the region. Variables with a statistically significant association with severe food insecurity in the region are: coffee cultivation (when market participation is low), dependence on agricultural labor income, and poverty level. Drivers of food insecurity experiences most commonly identified by participants are: consecutive drought; ill health and displacement of income for medicine; social marginalization; high start-up costs in production; absence or separation of a household head; and a lack of income and education opportunity. Ethnographic approaches identify a broader range of drivers contributing to food insecurity experiences, and add explanatory power to a statistical model of severe food insecurity. This integrated analysis provides a holistic picture of food insecurity in Guatemala's dry corridor region

A Revisit of the Two-Pole Caustic Model for GeV Light Curves of Gamma-Ray Pulsars

The GeV light curve of a pulsar is an important probe to detect acceleration regions in its magnetosphere. Motivated by the recent reports on the observations of pulsars by {\it Fermi} Large Area Telescope (LAT), we restudy the two-pole caustic model and revise it to investigate the properties of the light curves in the GeV band. In the revised model, although acceleration gaps can extend from the star surface to the light cylinder along near the last open field lines, the extension of the gaps along the azimuthal direction is limited because of photon-photon pair production process. In such gaps, high-energy photons are emitted uniformly and tangentially to the field lines but cannot be efficiently produced along these field lines where the distances to the null charge surface are larger than $\sim0.9$ times of the distance of the light cylinder, and the effective azimuth extension of the gaps is about $230^\circ$. The model is applied to the four pulsars Vela, PSR J1028-5819, PSR J0205+6449, and PSR J2021+3651 whose light curves obtained with {\it Fermi} have been recently released. The model is successful in reproducing the general feature of the light curves for the four pulsars, and the radial distances of the radio pulse for the four pulsars are estimated.Comment: A reference is correcte