60 research outputs found
Tandem leader proteases of Grapevine leafroll-associated virus-2: Host-specific functions in the infection cycle
AbstractSeveral viruses in the genus Closterovirus including Grapevine leafroll-associated virus-2 (GLRaV-2), encode a tandem of papain-like leader proteases (L1 and L2) whose functional profiles remained largely uncharacterized. We generated a series of the full-length, reporter-tagged, clones of GLRaV-2 and demonstrated that they are systemically infectious upon agroinfection of an experimental host plant Nicotiana benthamiana. These clones and corresponding minireplicon derivatives were used to address L1 and L2 functions in GLRaV-2 infection cycle. It was found that the deletion of genome region encoding the entire L1–L2 tandem resulted in a ~100-fold reduction in minireplicon RNA accumulation. Five-fold reduction in RNA level was observed upon deletion of L1 coding region. In contrast, deletion of L2 coding region did not affect RNA accumulation. It was also found that the autocatalytic cleavage by L2 but not by L1 is essential for genome replication. Analysis of the corresponding mutants in the context of N. benthamiana infection launched by the full-length GLRaV-2 clone revealed that L1 or its coding region is essential for virus ability to establish infection, while L2 plays an accessory role in the viral systemic transport. Strikingly, when tagged minireplicon variants were used for the leaf agroinfiltration of the GLRaV-2 natural host, Vitis vinifera, deletion of either L1 or L2 resulted in a dramatic reduction of minireplicon ability to establish infection attesting to a host-specific requirement for tandem proteases in the virus infection cycle
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Expression, Splicing, and Evolution of the Myosin Gene Family in Plants
Plants possess two myosin classes, VIII and XI. The myosins XI are implicated in organelle transport, filamentous actin
organization, and cell and plant growth. Due to the large size of myosin gene families, knowledge of these molecular motors
remains patchy. Using deep transcriptome sequencing and bioinformatics, we systematically investigated myosin genes in two
model plants, Arabidopsis (Arabidopsis thaliana) and Brachypodium (Brachypodium distachyon). We improved myosin gene
models and found that myosin genes undergo alternative splicing. We experimentally validated the gene models for
Arabidopsis myosin XI-K, which plays the principal role in cell interior dynamics, as well as for its Brachypodium ortholog. We
showed that the Arabidopsis gene dubbed HDK (for headless derivative of myosin XI-K), which emerged through a partial
duplication of the XI-K gene, is developmentally regulated. A gene with similar architecture was also found in Brachypodium.
Our analyses revealed two predominant patterns of myosin gene expression, namely pollen/stamen-specific and ubiquitous
expression throughout the plant. We also found that several myosins XI can be rhythmically expressed. Phylogenetic
reconstructions indicate that the last common ancestor of the angiosperms possessed two myosins VIII and five myosins XI,
many of which underwent additional lineage-specific duplications.This is the publisher’s final pdf. The published article is copyrighted by the American Society of Plant Biologists and can be found at: http://www.plantphysiol.org/
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Virus-Derived Gene Expression and RNA Interference Vector for Grapevine
The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction of desired traits into grapevine without heritable modifications to the genome. This vector expresses recombinant proteins in the phloem tissue that is involved in sugar transport throughout the plant, from leaves to roots to berries. Furthermore, the vector provides a powerful RNA interference (RNAi) capability of regulating the expression of endogenous genes via virus-induced gene-silencing (VIGS) technology. Additional advantages of this vector include superb genetic capacity and stability, as well as the swiftness of technology implementation. The most significant applications of the viral vector include functional genomics of the grapevine and disease control via RNAi-enabled vaccination against pathogens or invertebrate pests.This is the publisher’s final pdf. The published article is copyrighted by the American Society for Microbiology and can be found at: http://jvi.asm.org/. The authors have paid a fee to allow immediate free access to this article
Tubule-Guided Cell-to-Cell Movement of a Plant Virus Requires Class XI Myosin Motors
Cell-to-cell movement of plant viruses occurs via plasmodesmata (PD), organelles that evolved to facilitate intercellular communications. Viral movement proteins (MP) modify PD to allow passage of the virus particles or nucleoproteins. This passage occurs via several distinct mechanisms one of which is MP-dependent formation of the tubules that traverse PD and provide a conduit for virion translocation. The MP of tubule-forming viruses including Grapevine fanleaf virus (GFLV) recruit the plant PD receptors called Plasmodesmata Located Proteins (PDLP) to mediate tubule assembly and virus movement. Here we show that PDLP1 is transported to PD through a specific route within the secretory pathway in a myosin-dependent manner. This transport relies primarily on the class XI myosins XI-K and XI-2. Inactivation of these myosins using dominant negative inhibition results in mislocalization of PDLP and MP and suppression of GFLV movement. We also found that the proper targeting of specific markers of the Golgi apparatus, the plasma membrane, PD, lipid raft subdomains within the plasma membrane, and the tonoplast was not affected by myosin XI-K inhibition. However, the normal tonoplast dynamics required myosin XI-K activity. These results reveal a new pathway of the myosin-dependent protein trafficking to PD that is hijacked by GFLV to promote tubule-guided transport of this virus between plant cells
Movement Protein Pns6 of Rice dwarf phytoreovirus Has Both ATPase and RNA Binding Activities
Cell-to-cell movement is essential for plant viruses to systemically infect host plants. Plant viruses encode movement proteins (MP) to facilitate such movement. Unlike the well-characterized MPs of DNA viruses and single-stranded RNA (ssRNA) viruses, knowledge of the functional mechanisms of MPs encoded by double-stranded RNA (dsRNA) viruses is very limited. In particular, many studied MPs of DNA and ssRNA viruses bind non-specifically ssRNAs, leading to models in which ribonucleoprotein complexes (RNPs) move from cell to cell. Thus, it will be of special interest to determine whether MPs of dsRNA viruses interact with genomic dsRNAs or their derivative sRNAs. To this end, we studied the biochemical functions of MP Pns6 of Rice dwarf phytoreovirus (RDV), a member of Phytoreovirus that contains a 12-segmented dsRNA genome. We report here that Pns6 binds both dsRNAs and ssRNAs. Intriguingly, Pns6 exhibits non-sequence specificity for dsRNA but shows preference for ssRNA sequences derived from the conserved genomic 5′- and 3′- terminal consensus sequences of RDV. Furthermore, Pns6 exhibits magnesium-dependent ATPase activities. Mutagenesis identified the RNA binding and ATPase activity sites of Pns6 at the N- and C-termini, respectively. Our results uncovered the novel property of a viral MP in differentially recognizing dsRNA and ssRNA and establish a biochemical basis to enable further studies on the mechanisms of dsRNA viral MP functions
RNA-binding properties and membrane insertion of Melon necrotic spot virus (MNSV) double gene block movement proteins
Advances in structural and biochemical properties of carmovirus movement proteins (MPs) have only been obtained in p7 and p9 from Carnation mottle virus (CarMV). Alignment of carmovirus MPs revealed a low conservation of amino acid identity but interestingly, similarity was elevated in regions associated with the functional secondary structure elements reported for CarMV which were conserved in all studied proteins. Nevertheless, some differential features in relation with CarMV MPs were identified in those from Melon necrotic virus (MNSV) (p7A and p7B). p7A was a soluble non-sequence specific RNA-binding protein, but unlike CarMV p7, its central region alone could not account for the RNA-binding properties of the entire protein. In fact, a 22-amino acid synthetic peptide whose sequence corresponds to this central region rendered an apparent dissociation constant (K(d)) significantly higher than that of the corresponding entire protein (9 mM vs. 0.83-25.7 microM). This p7A-derived peptide could be induced to fold into an alpha-helical structure as demonstrated for other carmovirus p7-like proteins. Additionally, in vitro fractionation of p7B transcription/translation mixtures in the presence of ER-derived microsomal membranes strongly suggested that p7B is an integral membrane protein. Both characteristics of these two small MPs forming the double gene block (DGB) of MNSV are discussed in the context of the intra- and intercellular movement of carmovirus
The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes
Plant positive-strand RNA viruses require association with plant cell endomembranes for viral translation and replication, as
well as for intra- and intercellular movement of the viral progeny. The membrane association and RNA binding of the Tobacco
mosaic virus (TMV) movement protein (MP) are vital for orchestrating the macromolecular network required for virus movement.
A previously proposed topological model suggests that TMV MP is an integral membrane protein with two putative -helical
transmembrane (TM) segments. Here we tested this model using an experimental system that measured the efficiency with
which natural polypeptide segments were inserted into the ER membrane under conditions approximating the in vivo situation,
as well as in planta. Our results demonstrated that the two hydrophobic regions (HRs) of TMV MP do not span biological membranes.
We further found that mutations to alter the hydrophobicity of the first HR modified membrane association and precluded
virus movement. We propose a topological model in which the TMV MP HRs intimately associate with the cellular membranes,
allowing maximum exposure of the hydrophilic domains of the MP to the cytoplasmic cellular components.This work was supported by grants BFU2009-08401 and BFU2012-39482 (to I. M.) and BIO2011-25018 (to V. P.) from the Spanish MINECO. A. P. is the recipient of a JAE predoc position (CSIC).PeirĂł Morell, A.; MartĂnez-Gil, L.; Tamborero, S.; Pallás Benet, V.; Sanchez Navarro, JA.; Mingarro, I. (2014). The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes. Journal of Virology. 88(5):3016-3026. https://doi.org/10.1128/JVI.03648-13S3016302688
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