Genetic diversity of ancient grape cultivars of the Crimea region

A total of 76 accessions of Crimean autochthonous grape cultivars from the collection of the National Institute of Vine and Wine "Magarach" were genotyped using 22 nuclear and 3 chloroplast microsatellite loci (SSR) to characterize their genetic diversity. The total number of alleles was 238, the mean number of alleles per locus was 10.8 and the range of expected heterozygosity was 0.4-0.88. Several synonyms were identified based on the comparison of microsatellite profiles with INRA and EVD databases. Morphological, eno-carpological and eno-chemical characterization of number of cultivars using BBCH scale and OIV descriptors were performed. A short synopsis of the origin and historical development of Crimean autochthonous grape cultivars is presented

Introgressions of Vitis rotundifolia Michx. to obtain grapevine genotypes with complex resistance to biotic and abiotic stresses

Vitis rotundifolia Michx. is one of the species of the family Vitaceae, with resistance to both biotic and abiotic stresses. The present study reports new scientific knowledge about the inheritance of resistance to downy mildew, powdery mildew and frost by V. vinifera varieties from V. rotundifolia. Recombinant lines of three hybrid populations from the crossing of the maternal genotype ♀M. 31-77-10 with V. rotundifolia hybrids were used as the object of the study. As a result of laboratory screening, more than 40 % of recombinants of the ♀M. 31-77-10× ×[DRX-M5-734+DRX-M5-753+DRX-M5-790] population showed a high degree of frost resistance (–24 °C), while 6 % of transgressive recombinants were characterized by a very high degree of resistance (–27 °С). The maternal genotype ♀M. 31-77-10 does not carry alleles of resistance to powdery mildew at the Run1 locus and in the field suffers from powdery mildew much more than the paternal genotypes. The prevalence of powdery mildew on vegetative organs in the three recombinant populations over the years varies on average between 3.2–17.1, 0.3–17.7 and 0.6–5.2 %, respectively. As a result, almost all recombinant genotypes that received a resistant allele from the paternal genome are highly resistant to powdery mildew

Clinical Justification for Preliminary Thermal Exposure to Composite in the Treatment of Caries: Randomized Clinical Trial

Background. Polymer composites have good aesthetic characteristics and pronounced physicochemical properties, as compared to traditional restorative materials such as amalgam. However, the polymerization reaction of composite material containing bismethacrylate group commonly used in clinical practice is always accompanied by a different degree of volumetric shrinkage (2.7%~7.1%). The resulting stress can lead to adhesion failure and some other unfavorable clinical consequences, such as enamel destruction, microcracking of composite material and formation of microleakage between composite and tooth cavity wall, which can result in recurrent caries and postoperative sensitivity, thereby affecting the long-term effect of restoration. Therefore, studying the effect of preheating on composite restoration is important for its clinical application. Objective. To improve the effectiveness of treatment of patients with dental caries by improving the physicochemical properties of composite restorations.Methods. A randomized clinical trial enrolled 180 patients aged 18 to 45 years, diagnosed with dentin caries class I, according to Black (K02.1 in ICD). The study was conducted in the Dental Clinic of Kuban State Medical University, Russia. 180 composite restorations were performed in the treatment of dentin caries of molars. Patients were randomized into 2 groups): the control group — 90 patients and the main group — 90 patients. The control and main groups, in turn, were divided into three subgroups, depending on the composite used — Estelite Sigma Quick (Tokuyama Dental, Japan), Filtek Bulk Fill Posterior Restorative (3M Espe, USA) and DentLight (VladMiVa, Russia). Each subgroup consisted of 30 patients. In the control group, the classical method of filling with a composite material at “room temperature” was applied. In the main group, a composite heating conditioner “Ena Heat” (Micerium, Italy) was used to heat the composite to 55 °C before adapting the material in the formed cavity with subsequent photopolymerization. The quality of composite restorations within the clinical study was evaluated using the modified Ryge criterion immediately after treatment and after 6, 12, 18, 24 months. Statistical processing of the obtained data was carried out by means of one-factor analysis of variance using the GraphPadPrism 9 program (GraphPad Software, USA).Results. The duration of the clinical study comprised 24 months. The study revealed a statistically significant decrease in the quality of marginal fit of composite restorations (according to the Ryge score) by 20.1% (p = 0.0001) in the control group and by 5.7% (p = 0.0328) in the main group.  At the same time, no statistically significant changes in Ryge scores were reported in the subgroups of composite materials of the main group (pF = 0.9480, pE = 0.1837, pD = 0.2529). As a result of the study, an optimal time algorithm (7 seconds) for using a special furnace for preheating the composite before sealing with subsequent photopolymerization was obtained.Conclusion. The study revealed a statistically significant positive effect of the proposed algorithm for working with a heated composite on the quality of marginal fit in the long term after treatment in comparison with the classical method of working with a composite at “room temperature”

Description of the Vitis vinifera L. phenotypic variability in eno-carpological traits by a Euro-Asiatic collaborative network among ampelographic collections

The grapevine intra-specific variability captured an increasing interest during the last decades, as demonstrated by the number of recently funded European projects focused on the grapevine biodiversity preservation. However, nowadays, crop plants are mainly characterized by genotyping methods. The present work summarizes the phenotype data collected among 20 ampelographic collections spread in 15 countries, covering mostly of the viticultural areas in the Euro-Asiatic range: from Portugal to Armenia and from Cyprus to Luxembourg. Together with agro-climatic characterization of the experimental site, in two years, about 2400 accessions were described, following a common experimental protocol mainly focused on the carpological and oenological traits, obtaining a general overview of the distribution of the considered phenotypic traits in the cultivated Vitis vinifera species. The most replicated cultivars were selected and, for the subset of these reference cultivars, their behavior in the different environmental conditions over sites and years was described by ANOVA methods

Description of the vitis vinifera L. Phenotypic variability in eno-carpological traits by a Euro-Asiatic collaborative network among ampelographic collections

The grapevine intra-specific variability captured an increasing interest during the last decades, as demonstrated by the number of recently funded European projects focused on the grapevine biodiversity preservation. However, nowadays, crop plants are mainly characterized by genotyping methods. The present work summarizes the phenotype data collected among 20 ampelographic collections spread over 15 countries, covering most of the viticultural areas in the Euro-Asiatic region: from Portugal to Armenia and from Cyprus to Luxembourg. Together with agro-climatic characterization of the experimental site, over two years about 2,400 accessions were described. A common experimental protocol mainly focused on the carpological and oe-nological traits was followed, obtaining a general overview of the distribution of the considered phenotypic traits in the cultivated Vitis vinifera species. The most replicated cultivars were selected and, for the subset of these reference cultivars, their behavior in the different environmental conditions over sites and years was described by ANOVA methods

BACTERIOTOXIC LIGHTTHERAPY APPLICATION IN COMPLEX CURE OF PARODONT TISSUES” INFLAMMATORY DISEASES

The prevalence of periodontal diseases of varying severity affects more than 90% of the adult population, and the problem of increasing the effectiveness of its treatment is very important. One of the promising methods of treatment is antibiotic therapy with the use of probiotic microorganisms and antioxidants. The contomitant use effectiveness of antibacterial BTC-therapy, Linex probiotics® and Xylax forte®, antioxidant ubixion (coenzim Q 10) for microbiocenosis gingival pockets' sanation was investigated on 60 patients with inveterate severe generalized periodontitis. It was found that pathogenic microflora volume decreased in 21,3±0,7(

Genetic diversity of ancient grape cultivars of the Crimea region

UMR AGAP équipe DAAV (Diversité, adaptation et amélioration de la vigne)International audienceA total of 76 accessions of Crimean autochthonous grape cultivars from the collection of the National Institute of Vine and Wine "Magarach" were genotyped using 22 nuclear and 3 chloroplast microsatellite loci (SSR) to characterize their genetic diversity. The total number of alleles was 238, the mean number of alleles per locus was 10.8 and the range of expected heterozygosity was 0.4-0.88. Several synonyms were identified based on the comparison of microsatellite profiles with INRA and EVD databases. Morphological, eno-carpo-logical and eno-chemical characterization of number of cultivars using BBCH scale and OIV descriptors were performed. A short synopsis of the origin and historical development of Crimean autochthonous grape cultivars is presented

FEATURES OF USING NONSTEROIDAL ANTI-INFLAMMATORY DRUGS IN DENTAL PRACTICE

Aim. This study was conducted to assess the awareness of dentists about the questions of pharmacokinetics and pharmacodynamics of nonsteroidal anti-inflammatory drugs as well as to study the features of their use for the relief of pain syndrome in dental practice.Materials and methods. By means of the questionnaires were studied 107 dentists working in dental clinics and dental departments of General hospitals, especially their use of the nonsteroidal anti-inflammatory drugs for pain relief.Results. It was determined that 85% of doctors use non-selective and moderately selective cyclooxygenase inhibitors (COX), 15%-prefer Cox-2 inhibitor with the pronounced selectivity for pain relief. The highest number of correct answers was given by a group of doctors with work experience from 5 to 10 years (40.3%), the lowest – by doctors with work experience more than 20 years, respectively 40.3% and 36.1% of the total number of doctors from the studied groups.Conclusion. There was noted that it is necessary to pay attention to the peculiarities of pain relief for the improvement of dentists’ work as well as to involve clinical pharmacologists for these purposes