Fluorescent Plasmodium berghei sporozoites and pre-erythrocytic stages: a new tool to study mosquito and mammalian host interactions with malaria parasites.

To track malaria parasites for biological studies within the mosquito and mammalian hosts, we constructed a stably transformed clonal line of Plasmodium berghei, PbFluspo, in which sporogonic and pre-erythrocytic liver-stage parasites are autonomously fluorescent. A cassette containing the structural gene for the FACS-adapted green fluorescent protein mutant 2 (GFPmut2), expressed from the 5' and 3' flanking sequences of the circumsporozoite (CS) protein gene, was integrated and expressed at the endogenous CS locus. Recombinant parasites, which bear a wild-type copy of CS, generated highly fluorescent oocysts and sporozoites that invaded mosquito salivary glands and were transmitted normally to rodent hosts. The parasites infected cultured hepatocytes in vitro, where they developed into fluorescent pre-erythrocytic forms. Mammalian cells infected by these parasites can be separated from non-infected cells by fluorescence activated cell sorter (FACS) analysis. These fluorescent insect and mammalian stages of P. berghei should be useful for phenotypic studies in their respective hosts, as well as for identification of new genes expressed in these parasite stages

Z Cam stars: a particular response to a general phenomenon

We show that the disc instability model can reproduce all the observed properties of Z Cam stars if the energy equation includes heating of the outer disc by the mass-transfer stream impact and by tidal torques and if the mass-transfer rate from the secondary varies by about 30% around the value critical for stability. In particular the magnitude difference between outburst maxima and standstills corresponds to observations, all outbursts are of the inside-out type and can be divided into two classes: long (wide) and short (narrow) outbursts, as observed. Mass transfer rate fluctuations should occur in other dwarf novae but one can exclude variations similar to those observed in magnetic systems (AM Her's and some DQ Her's) and some nova-like systems (VY Scl's), in which \dot{M} become very small during low states; these would produce mini-outburst which, although detectable, have never been observed.Comment: submitted to Astronomy & Astrophysics, 8 pages, 4 figure

The Connections between QSO Absorption Systems and Galaxies: Low-Redshift Observations

Quasar absorption lines have long been recognized to be a sensitive probe of the abundances, physical conditions, and kinematics of gas in a wide variety of environments including low-density intergalactic regions that probably cannot be studied by any other means. While some pre-Hubble Space Telescope (HST) observations indicated that Mg II absorption lines arise in gaseous galactic halos with a large covering factor, many early QSO absorber studies were hampered by a lack of information about the context of the absorbers and their connections with galaxies. By providing access to crucial ultraviolet resonance lines at low redshifts, deployment of HST and the Far Ultraviolet Spectroscopic Explorer enabled detailed studies of the relationships between QSO absorbers and galaxies. The advent of large surveys such as the Sloan Digital Sky Survey (SDSS) has also advanced the topic by greatly improving the size of absorber and galaxy samples. This paper briefly reviews some observational results on absorber-galaxy connections that have been obtained in the HST/SDSS era, including Mg II absorbers, the low-z Lyman alpha forest, Lyman limit and damped Lyman alpha absorbers, and O VI systems.Comment: Review paper presented at IAU Colloquium 199, Probing Galaxies through Quasar Absorption Lines, eds. P. R. Williams, C. Shu, and B. Menard. 19 pages, 10 figure

Long range coherent magnetic bound states in superconductors

The quantum coherent coupling of completely different degrees of freedom is a challenging path towards creating new functionalities for quantum electronics. Usually the antagonistic coupling between spins of magnetic impurities and superconductivity leads to the destruction of the superconducting order. Here we show that a localized classical spin of an iron atom immersed in a superconducting condensate can give rise to new kind of long range coherent magnetic quantum state. In addition to the well-known Shiba bound state present on top of an impurity we reveal the existence of a star shaped pattern which extends as far as 12 nm from the impurity location. This large spatial dispersion turns out to be related, in a non-trivial way, to the superconducting coherence length. Inside star branches we observed short scale interference fringes with a particle-hole asymmetry. Our theoretical approach captures these features and relates them to the electronic band structure and the Fermi wave length of the superconductor. The discovery of a directional long range effect implies that distant magnetic atoms could coherently interact leading to new topological superconducting phases with fascinating properties

Herschel PACS Observations and Modeling of Debris Disks in the Tucana-Horologium Association

We present Herschel PACS photometry of seventeen B- to M-type stars in the 30 Myr-old Tucana-Horologium Association. This work is part of the Herschel Open Time Key Programme "Gas in Protoplanetary Systems" (GASPS). Six of the seventeen targets were found to have infrared excesses significantly greater than the expected stellar IR fluxes, including a previously unknown disk around HD30051. These six debris disks were fitted with single-temperature blackbody models to estimate the temperatures and abundances of the dust in the systems. For the five stars that show excess emission in the Herschel PACS photometry and also have Spitzer IRS spectra, we fit the data with models of optically thin debris disks with realistic grain properties in order to better estimate the disk parameters. The model is determined by a set of six parameters: surface density index, grain size distribution index, minimum and maximum grain sizes, and the inner and outer radii of the disk. The best fitting parameters give us constraints on the geometry of the dust in these systems, as well as lower limits to the total dust masses. The HD105 disk was further constrained by fitting marginally resolved PACS 70 micron imaging.Comment: 15 pages, 7 figures, Accepted to Ap

Precise and simple quantitation of liver steatosis by MRI in an animal model and in patients with NAFLD

International audienc

On the Connection Between Metal Absorbers and Quasar Nebulae

We establish a simple model for the distribution of cold gas around L* galaxies using a large set of observational constraints on the properties of strong MgII absorber systems. Our analysis suggests that the halos of L* galaxies are filled with cool gaseous clouds having sizes of order 1kpc and densities of ~10^{-2} cm^{-3}. We then investigate the physical effects of cloud irradiation by a quasar and study the resulting spectral signatures. We show that quasar activity gives rise to (i) extended narrow-line emission on ~100kpc scales and (ii) an anisotropy in the properties of the absorbing gas arising from the geometry of the quasar radiation field. Provided that quasars reside in halos several times more massive than those of L* galaxies, our model predictions appear to be in agreement with observations of narrow emission-line nebulae around quasars and the recent detections of ~100kpc cold gaseous envelopes around those objects, suggesting a common origin for these phenomena. We discuss the implications of our results for understanding absorption systems, probing quasar environments at high redshifts, and testing the quasar unification scheme.Comment: 15 pages, 13 figures (ApJ submitted

A model of superoutbursts in binaries of SU UMa type

A new mechanism explaining superoutbursts in binaries of SU UMa type is proposed. In the framework of this mechanism the accretion rate increase leading to the superoutburst is associated with formation of a spiral wave of a new "precessional" type in inner gasdynamically unperturbed parts of the accretion disc. The possibility of existence of this type of waves was suggested in our previous work (astro-ph/0403053). The features of the "precessional" spiral wave allow explaining both the energy release during the outburst and all its observational manifestations. The distinctive characteristic of a superoutburst in a SU UMa type star is the appearance of the superhump on the light curve. The proposed model reproduces well the formation of the superhump as well as its observational features, such as the period that is 3-7% longer than the orbital one and the detectability of superhumps regardless of the binary inclination.Comment: LaTeX, 20 pages, 4 figures, to be published in Astron. Z

Governing cooperative quality schemes: some lessons from olive oil initiatives in the Region of Valencia (Spain)

This paper describes the development, functioning and governance of quality assurance schemes for olive oil in three cooperative organizations. Two questions are addressed: first, how the construction of new quality schemes by cooperatives can lead to the fragmentation of the membership base, and hence the need for new institutional arrangements; and second, how the development of such schemes alters the interdependencies between parties (cooperative organization and its members), and how this is governed. The comparative analysis shows the relevance of the social and cultural contexts in shaping the mechanisms of governance, as well contributing to the policy debate in the European Union regarding new food quality schemes.Moragues Faus, AM.; Ortiz Miranda, D. (2012). Governing cooperative quality schemes: some lessons from olive oil initiatives in the Region of Valencia (Spain). Outlook On Agriculture. 41(1):27-33. doi:10.5367/oa.2012.0072S273341

Superoutbursts, superhumps and the tidal-thermal instability model

We include the tidal instability due to the 3:1 resonance in the disc instability model developed by Hameury et al. (1998) and modified by Buat-Menard et al. (2001). We confirm earlier results by Osaki (1989) that the tidal instability can account for the SU UMa light curves. We show that in ultra-low mass ratio systems such as ER UMa stars and WZ Sge stars, the superoutburst ends while the disc is still eccentric, as proposed by Hellier (2001). However, since the disc shrinks rapidly once a cooling wave has started, the eccentricity should stop shortly after the end of a superoutburst. This result disagrees with the suggestion by Hellier that decoupling the thermal and tidal instability in the TTI model can account for late superhumps and echo outbursts in ultra-low mass ratio systems. We propose instead that ER UMa short supercycles can be explained either by the alternation of narrow and wide outbursts similar to those occurring in SS Cyg, or by the effects of irradiation (Hameury et al. 2000). In both cases, we predict that superhumps should be permanent, which is suggested by observations (Gao et al. 1999). We can also reproduce light curves similar to those of EG Cnc, varying the mass transfer rate in a TTI model including both irradiation and the presence of an inner hole in the disc.Comment: Astronomy and Astrophysics, submitted. 8 pages, 5 figure