8 research outputs found
Effects of ribosomes on the kinetics of Qβ replication
AbstractBacteriophage Qβ utilizes some host cell translation factors during replication. Previously, we constructed a kinetic model that explains replication of long RNA molecules by Qβ replicase. Here, we expanded the previous kinetic model to include the effects of ribosome concentration on RNA replication. The expanded model quantitatively explained single- and double-strand formation kinetics during replication with various ribosome concentrations for two artificial long RNAs. This expanded model and the knowledge obtained in this study provide useful frameworks to understand the precise replication mechanism of Qβ replicase with ribosomes and to design amplifiable RNA genomes in translation-coupling systems
Adaptive Evolution of an Artificial RNA Genome to a Reduced Ribosome Environment
The reconstitution
of an artificial system that has the same evolutionary
ability as a living thing is a major challenge in the <i>in vitro</i> synthetic biology. In this study, we tested the adaptive evolutionary
ability of an artificial RNA genome replication system, termed the
translation-coupled RNA replication (TcRR) system. In a previous work,
we performed a study of the long-term evolution of the genome with
an excess amount of ribosome. In this study, we continued the evolution
experiment in a reduced-ribosome environment and observed that the
mutant genome compensated for the reduced ribosome concentration.
This result demonstrated the ability of the TcRR system to adapt and
may be a step toward generating living things with evolutionary ability