Adaptive Evolution of an Artificial RNA Genome to
a Reduced Ribosome Environment
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Abstract
The reconstitution
of an artificial system that has the same evolutionary
ability as a living thing is a major challenge in the <i>in vitro</i> synthetic biology. In this study, we tested the adaptive evolutionary
ability of an artificial RNA genome replication system, termed the
translation-coupled RNA replication (TcRR) system. In a previous work,
we performed a study of the long-term evolution of the genome with
an excess amount of ribosome. In this study, we continued the evolution
experiment in a reduced-ribosome environment and observed that the
mutant genome compensated for the reduced ribosome concentration.
This result demonstrated the ability of the TcRR system to adapt and
may be a step toward generating living things with evolutionary ability