1,611 research outputs found
Convergent evolution of heat-inducibility during subfunctionalization of the Hsp70 gene family
Background: Heat-shock proteins of the 70 kDa family (Hsp70s) are essential chaperones required for key cellular functions. In eukaryotes, four subfamilies can be distinguished according to their function and localisation in different cellular compartments: cytosol, endoplasmic reticulum, mitochondria and chloroplasts. Generally, multiple cytosol-type Hsp70s can be found in metazoans that show either constitutive expression and/or stress-inducibility, arguing for the evolution of different tasks and functions. Information about the hsp70 copy number and diversity in microbial eukaryotes is, however, scarce, and detailed knowledge about the differential gene expression in most protists is lacking. Therefore, we have characterised the Hsp70 gene family of Paramecium caudatum to gain insight into the evolution and differential heat stress response of the distinct family members in protists and to investigate the diversification of eukaryotic hsp70s focusing on the evolution of heat-inducibility.
Results: Eleven putative hsp70 genes could be detected in P. caudatum comprising homologs of three major Hsp70-subfamilies. Phylogenetic analyses revealed five evolutionarily distinct Hsp70-groups, each with a closer relationship to orthologous sequences of Paramecium tetraurelia than to another P. caudatum Hsp70-group. These highly diverse, paralogous groups resulted from duplications preceding Paramecium speciation, underwent divergent evolution and were subject to purifying selection. Heat-shock treatments were performed to test for differential expression patterns among the five Hsp70-groups as well as for a functional conservation within Paramecium. These treatments induced exceptionally high mRNA up-regulations in one cytosolic group with a low basal expression, indicative for the major heat inducible hsp70s. All other groups showed comparatively high basal expression levels and moderate heat-inducibility, signifying constitutively expressed genes. Comparative EST analyses for P. tetraurelia hsp70s unveiled a corresponding expression pattern, which supports a functionally conserved evolution of the Hsp70 gene family in Paramecium.
Conclusions: Our analyses suggest an independent evolution of the heat-inducible cytosol-type hsp70s in Paramecium and in its close relative Tetrahymena, as well as within higher eukaryotes. This result indicates convergent evolution during hsp70 subfunctionalization and implies that heat-inducibility evolved several times during the course of eukaryotic evolution
Chandra Observation of the X-Ray Source Population of NGC 6946
We present the results of a study of discrete X-ray sources in NGC 6946 using
a deep Chandra ACIS observation. Based on the slope of the log N-log S
distribution and the general correlation of sources with the spiral arms, we
infer that the overall discrete source sample in NGC 6946 is dominated by high
mass X-ray binaries, in contrast to the source distributions in M31 and the
Milky Way. This is consistent with the higher star formation rate in NGC 6946
than in those galaxies. We find that the strong X-ray sources in the region of
the galactic center do not correlate in detail with images of the region in the
near-IR, although one of them may be coincident with the galactic center. The
non-central ultra-luminous X-ray source in NGC 6946, previously identified with
a supernova remnant, has an X-ray spectrum and luminosity that is inconsistent
with either a traditional pulsar wind nebula or a blast wave remnant.Comment: accepted Ap
Audition in vampire bats, Desmodus rotundus
1. Within the tonotopic organization of the inferior colliculus two frequency ranges are well represented: a frequency range within that of the echolocation signals from 50 to 100 kHz, and a frequency band below that of the echolocation sounds, from 10 to 35 kHz. The frequency range between these two bands, from about 40 to 50 kHz is distinctly underrepresented (Fig. 3B).
2. Units with BFs in the lower frequency range (10–25 kHz) were most sensitive with thresholds of -5 to -11 dB SPL, and units with BFs within the frequency range of the echolocation signals had minimal thresholds around 0 dB SPL (Fig. 1).
3. In the medial part of the rostral inferior colliculus units were encountered which preferentially or exclusively responded to noise stimuli. — Seven neurons were found which were only excited by human breathing noises and not by pure tones, frequency modulated signals or various noise bands. These neurons were considered as a subspeciality of the larger sample of noise-sensitive neurons. — The maximal auditory sensitivity in the frequency range below that of echolocation, and the conspicuous existence of noise and breathing-noise sensitive units in the inferior colliculus are discussed in context with the foraging behavior of vampire bats
From X-ray dips to eclipse: Witnessing disk reformation in the recurrent nova USco
The 10th recorded outburst of the recurrent eclipsing nova USco was observed
simultaneously in X-ray, UV, and optical by XMM-Newton on days 22.9 and 34.9
after outburst. Two full passages of the companion in front of the nova ejecta
were observed, witnessing the reformation of the accretion disk. On day 22.9,
we observed smooth eclipses in UV and optical but deep dips in the X-ray light
curve which disappeared by day 34.9, then yielding clean eclipses in all bands.
X-ray dips can be caused by clumpy absorbing material that intersects the line
of sight while moving along highly elliptical trajectories. Cold material from
the companion could explain the absence of dips in UV and optical light. The
disappearance of X-ray dips before day 34.9 implies significant progress in the
formation of the disk. The X-ray spectra contain photospheric continuum
emission plus strong emission lines, but no clear absorption lines. Both
continuum and emission lines in the X-ray spectra indicate a temperature
increase from day 22.9 to day 34.9. We find clear evidence in the spectra and
light curves for Thompson scattering of the photospheric emission from the
white dwarf. Photospheric absorption lines can be smeared out during scattering
in a plasma of fast electrons. We also find spectral signatures of resonant
line scattering that lead to the observation of the strong emission lines.
Their dominance could be a general phenomenon in high-inclination systems such
as Cal87.Comment: Submitted to ApJ. 16 pages, 16 figure
ConXsense - Automated Context Classification for Context-Aware Access Control
We present ConXsense, the first framework for context-aware access control on
mobile devices based on context classification. Previous context-aware access
control systems often require users to laboriously specify detailed policies or
they rely on pre-defined policies not adequately reflecting the true
preferences of users. We present the design and implementation of a
context-aware framework that uses a probabilistic approach to overcome these
deficiencies. The framework utilizes context sensing and machine learning to
automatically classify contexts according to their security and privacy-related
properties. We apply the framework to two important smartphone-related use
cases: protection against device misuse using a dynamic device lock and
protection against sensory malware. We ground our analysis on a sociological
survey examining the perceptions and concerns of users related to contextual
smartphone security and analyze the effectiveness of our approach with
real-world context data. We also demonstrate the integration of our framework
with the FlaskDroid architecture for fine-grained access control enforcement on
the Android platform.Comment: Recipient of the Best Paper Awar
Internal kinematics of spiral galaxies in distant clusters. Part II. Observations and data analysis
We have conducted an observing campaign with FORS at the ESO-VLT to explore
the kinematical properties of spiral galaxies in distant galaxy clusters. Our
main goal is to analyse transformation- and interaction processes of disk
galaxies within the special environment of clusters as compared to the
hierarchical evolution of galaxies in the field. Spatially resolved MOS-spectra
have been obtained for seven galaxy clusters at 0.3<z<0.6 to measure rotation
velocities of cluster members. For three of the clusters, Cl0303+17, Cl0413-65,
and MS1008-12, for which we presented results including a TF-diagram in Ziegler
et al. 2003, we describe here in detail the observations and data analysis.
Each of them was observed with two setups of the standard FORS MOS-unit.With
typical exposure times of >2 hours we reach an S/N>5 in the emission lines
appropriate for the deduction of the galaxies' internal rotation velocities
from [OII], Hbeta, or [OIII] profiles. Preselection of targets was done on the
basis of available redshifts as well as from photometric and morphological
information gathered from own observations, archive data, and from the
literature. Emphasis was laid on the definition of suitable setups to avoid the
typical restrictions of the standard MOS unit for this kind of observations. In
total we assembled spectra of 116 objects of which 50 turned out to be cluster
members. Position velocity diagrams, finding charts as well as tables with
photometric, spectral, and structural parameters of individual galaxies are
presented.Comment: 18 pages, 6 figures, accepted for publication in Astronomy and
Astrophysics. A version with full resolution figures can be downloaded from
http://www.uni-sw.gwdg.de/~vwgroup/publications.htm
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