Studies on isolation and characterisation and its effect of seed inoculation of PGPR (Pseudomonas fluorescens) on yield of tomato

Soil samples of five different locations of rhizosphere of tomato.  The sample used for identification of Pseudomonas fluorescens and characterisation based on biochemical characteristics.  The best isolate PF-5 used for seed treatment of with tomato variety PKM-1.  The higher fruit yield was recorded in the triple combination of Pseudomonas fluorescens + Azotobacterchroococcum + Azospirillum brasilense @ 900g/plant-1 followed by Pseudomonas + Azotobacter

Cost-effectiveness of interventions to control cardiovascular diseases and type 2 diabetes mellitus in South Asia: protocol for a systematic review.

INTRODUCTION: While a number of strategies are being implemented to control cardiovascular diseases (CVDs) and type 2 diabetes mellitus (T2DM), the cost-effectiveness of these in the South Asian context has not been systematically evaluated. We aim to systematically review the economic (cost-effectiveness) evidence available on the individual-, group- and population-level interventions for control of CVD and T2DM in South Asia. METHODS AND ANALYSIS: This review will consider all relevant economic evaluations, either conducted alongside randomised controlled trials or based on decision modelling estimates. These studies must include participants at risk of developing CVD/T2DM or with established disease in one or more of the South Asian countries (India, Bangladesh, Pakistan, Sri Lanka, Nepal, Maldives, Bhutan and Afghanistan). We will identify relevant papers by systematically searching all major databases and registries. Selected articles will be screened by two independent researchers. Methodological quality of the studies will be assessed using a modified Drummond and a Phillips checklist. Cochrane guidelines will be followed for bias assessment in the effectiveness studies. RESULTS: Results will be presented in line with the PRISMA (Preferred Reporting Items for Systematic review and Meta-analysis) checklist, and overall quality of evidence will be presented as per the GRADE (Grades of Recommendation, Assessment, Development and Evaluation) approach. ETHICS AND DISSEMINATION: The study has received ethics approval from the All India Institute of Medical Sciences, New Delhi, India. The results of this review will provide policy-relevant recommendations for the uptake of cost-effectiveness evidence in prioritising decisions on essential chronic disease care packages for South Asia. STUDY REGISTRATION NUMBER: PROSPERO CRD42013006479

A novel Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite and its biological applications : preparation and characterization

A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein were developed. The silver nanoparticles were prepared using the carrageenan polymer as reduction and capping agent. Here, the Ag/Carrageenan was combined with gelatin hydrogel using glutaraldehyde having a cross-link role in order to create the biohybrid hydrogel nanocomposite. The manufactured composite performances were anaylised by UV-visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods. The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite was also analyzed. The antibacterial activity was tested against human pathogens viz. S.agalactiae 1661, S. pyogenes 1210 and E. coli. The bacterial cell wall damage of S.agalactiae 1661 was analyzed by scanning electron microscopy. The cytotoxic assay was performed against the A549 lung cancer cells

Adoption of sea cage farming of cobia (Rachycentron canadum) by fishermen self-help groups as a diversified livelihood option: A success story from Ramanathapuram District, Tamil Nadu, India

The ICAR-Central Marine Fisheries Research Institute (ICAR-CMFRI) is the pioneer in developing the technology for cage fish farming in India. Cobia (Rachycentron canadum) grows relatively fast in cages and is very much suited for small scale mariculture to meet the alternate livelihood needs of fisherfolk. Mandapam Regional Centre of ICAR-CMFRI has developed the technology for breeding, seed production and farming of cobia in cages and organised training, and frontline/participatory demonstration programmes which have aided in creating awareness about the techno economic viability of cage farming among the fisherfolk in Ramanathapuram District, Tamil Nadu. A survey was taken up among a sample of 75 members of fisher self help groups (SHGs) who had adopted cage farming of cobia, in order to assess their perception and attributes about the technology as well as the constraints faced by them. The study found that fast growth rate of cobia, shorter culture period, good farm gate price, high market demand, ease of farming near the seashore and seed availability were the important attributes which attracted the fishermen groups to take up the farming. The innovations introduced by the fishers in cage farming technology were also documented. Majority of the fishers stated, non-availability of seed during peak stocking season, high cost of pellet feed, fluctuating cost of low value fishes which are used as feed for cobia and poaching as major constraints in cage farming

Microalgae as live feed

The development of mariculture is fully dependent on the availability seed of the fish or shell fish to be cultured. For this, hatchery production of the seed is imperative owing to the uncertain nature of natural seed resources. At present the seed production technology of some species with mariculture potential have been developed in India, which includes Cobia, Rachycentron canadum, Silver/Snubnose pompano, Trachinotus blochii, Indian pompano, T.mookalee, Orange spotted grouper, Epinephelus coioides, Sea bream, Lethrinus lentjan, Asian sea bass, Lates calcarifer etc

CCL5 regulation of mucosal chlamydial immunity and infection

<p>Abstract</p> <p>Background</p> <p>Following genital chlamydial infection, an early T helper type 1 (Th1)-associated immune response precedes the activation and recruitment of specific Th1 cells bearing distinct chemokine receptors, subsequently leading to the clearance of <it>Chlamydia</it>. We have shown that CCR5, a receptor for CCL5, is crucial for protective chlamydial immunity. Our laboratory and others have also demonstrated that CCL5 deficiencies found in man and animals can increase the susceptibility and progression of infectious diseases by modulating mucosal immunity. These findings suggest the CCR5-CCL5 axis is necessary for optimal chlamydial immunity. We hypothesized CCL5 is required for protective humoral and cellular immunity against <it>Chlamydia</it>.</p> <p>Results</p> <p>The present study revealed that CCR5 and CCL5 mRNAs are elevated in the spleen, iliac lymph nodes (ILNs), and genital mucosa following <it>Chlamydia muriduram </it>challenge. Antibody (Ab)-mediated inhibition of CCL5 during genital chlamydial infection suppressed humoral and Th1 > Th2 cellular responses by splenic-, ILN-, and genital mucosa-derived lymphocytes. Antigen (Ag)-specific proliferative responses of CD4⁺T cells from spleen, ILNs, and genital organs also declined after CCL5 inhibition.</p> <p>Conclusion</p> <p>The suppression of these responses correlated with delayed clearance of <it>C. muriduram</it>, which indicate chlamydial immunity is mediated by Th1 immune responses driven in part by CCL5. Taken together with other studies, the data show that CCL5 mediates the temporal recruitment and activation of leukocytes to mitigate chlamydial infection through enhancing adaptive mucosal humoral and cellular immunity.</p

2D Materials-based platforms for electroanalysis applications

A new class of nanomaterials called "2D materials" (2DMs) is attracting recently the electrochemical sensing field due to the unique physicochemical properties associated to their chemical structure, formed by ultra-thin layers. In this review, we summarize the recent advances in the electroanalysis area using 2DMs giving first a brief overview on the structure, synthesis and properties of these materials followed by the analysis of their advantages while used in the development of electrochemical sensors

In situ formation of 1D nanostructures from ceria nanoparticle dispersions by liquid cell TEM irradiation

Deliberate electron irradiation of cerium oxide nanoparticles in water is used to trigger chemical reactions in a liquid cell transmission electron microscope. Formation of nanorods and nanoneedles is observed starting from predominantly octahedral shape nanoparticles. Detailed morphologies found include free-standing needles, needles connected to specific octahedral ceria facets and star-shaped multi-needle patterns. It is found that rod-axis orientations and crystallographic directions are aligned. It is suggested that high ion and radical concentration of radiolysed water dissolves layers of the original CeO2 particles which re-arrange as needles in the direction of energetically preferred facets

New Insights into the Mechanism of Visible Light Photocatalysis

ABSTRACT: In recent years, the area of developing visible-lightactive photocatalysts based on titanium dioxide has been enormously investigated due to its wide range of applications in energy and environment related fields. Various strategies have been designed to efficiently utilize the solar radiation and to enhance the efficiency of photocatalytic processes. Building on the fundamental strategies to improve the visible light activity of TiO2-based photocatalysts, this Perspective aims to give an insight into many contemporary developments in the field of visible-light-active photocatalysis. Various examples of advanced TiO2 composites have been discussed in relation to their visible light induced photoconversion efficiency, dynamics of electron− hole separation, and decomposition of organic and inorganic pollutants, which suggest the critical need for further development of these types of materials for energy conversion and environmental remediation purposes