Studying Revenge in The Scarlet Letter and Moby Dick

This essay considers the theme of revenge in two novels: The Scarlet Letter by Nathaniel Hawthorne and Moby Dick by Herman Melville. In The Scarlet Letter, Roger Chillingworth finds out that he has been wronged by the priest Arthur Dimmesdale, who has had a relationship with Chillingworth’s wife, Hester Prynne. Hester’s daughter Pearl is born out of this relationship, and Hester has to spend a term in jail, stand on the scaffold for three hours, and take all the blame of the merciless Puritan community in Boston. According to Chillingworth, this is also enough to justify inflicting all sorts of pains on the priest or even killing him. Of course, Hester is not exempted from Chillingworth’s wrath, but as she is exposed to public shame, Chillingworth decides that that is enough for her and that he must direct his revenge upon her partner, who decides to keep his sin a secret. In Moby Dick, Captain Ahab, who has lost his leg while hunting the white whale Moby Dick, does not take the loss of his leg to Moby Dick as an accident that might befall any whaler. This loss touches his deepest sense of honour, and he decides to avenge himself upon the whale. He feels insulted and the artificial leg, which replaces his real one, works as a constant reminder of his wounded pride and urges him to go on in his revenge scheme. The two men’s schemes of revenge reach extreme levels. This essay will compare the concept of revenge between Hawthorne and Melville as they explore it in the presentation of the characters of Ahab and Chillingworth

Rancang Bangun Sistem Distribusi Hasil Produksi Obat untuk Menjamin Kualitas dalam Meningkatkan Pelayanan Pelanggan

Pharmaceutical companies in Indonesia that are engaged in manufacturing currently continue to develop, they process medicinal ingredients into pharmaceuticals, such as tablets, syrup, capsules and others. At present the distribution and production management system of drugs is still semi-computerized, because it still uses Ms. Excel, which can cause file-to-file buildup, besides that it is possible that the data will be lost or damaged, and when searching for data, it takes a long time. To overcome this need to be built a system to process the drug distribution and production management system, to help companies more easily access drug data to computerized travel documents, so as to increase the effectiveness and time efficiency, and get maximum work results and data accuracy in data input in the relevant section. It certainly can be found with various methods that use penuls, starting from the use of uml to provide an overview of system design, then MySql as a data processing application in thedatabase, as a programming language using php

Urban micro climate performance in deferent urban fabric in Mosul, Iraq

City of Mosul has a traditional urban core called old city, side by side with a modern fabric adopted by municipal authority since the rapid urbanization which the city had witnessed in the fifties of the last century, the traditional one characterizes with a compact and organic tissue, Introverted courtyard buildings built with a heavy thick masonry structure, while the modern is open geometric one with a wide streets and extraverted buildings build with concrete. City of Mosul has a hot dry climate summers and cool rainy winter, this research aims to make a comparative study for the urban micro climate performance (air temperature and relative humidity) in the two deferent urban fabrics (traditional and modern), during both the hottest and the coldest period of the year, using the meteorological data as a reference for evaluating the performance. The way cities are planned and built is therefore important for the global energy use environmental comfort. So it’s important to study the relationship between urban form and outdoor climate. The preliminary results confirm that the climatic conditions are much more stable in the traditional city than in the modern part of the city, regarding both air temperature and relative humidity

On the Behavior of the Nonlinear Difference Equation yn+1 = Ayn−1 + Byn−3 + Cyn−1+Dyn−3/Fyn−3−E

The theory of difference equations got a significant position in the applicable analysis. Therefore, studying the qualitative behavior of the difference equations is a fruitful area of research that has increasingly attracted many researchers. In this paper, we demonstrate the stability and the existence of periodic solutions of the nonlinear difference equation. Moreover, we provide some numerical simulations to confirm our results

Mechanisms of Groucho-mediated repression revealed by genome-wide analysis of Groucho binding and activity

Antibody validation (A) Chromatin isolated and sheared exactly as for the ChIP-seq analysis was subjected to immunoprecipitation with the indicated amounts (in μl) of affinity purified antibody against the Gro GP domain used for the ChIP-seq analysis, and then probed in a western blot with both an anti-Gro monoclonal antibody (mAb) or the anti-GP antibody. The band indicated by the asterisk is a cross-reacting protein that is recognized in the western blot but that is not efficiently immunoprecipitated by the anti-GP antibody. Ab HC – antibody heavy chain. (B) Heat map showing overlap (Jacard similarity coefficient [96]) between the peaks called in the duplicate ChIP-seq experiments at each time point. (C) Representative genome browser tracts comparing duplicate ChIP-seq experiments. (D and E) Comparison of Gro binding patterns obtained by ChIP-seq using our anti-GP antibody with that obtained by ChIP-chip (0–12 hr embryos; modENCODE #597) and ChIP-seq (white pre-pupae; modENCODE #4981) using independently derived antibodies [40]. (PDF 588 kb

Poultry and Beef Meat as Potential Seedbeds for Antimicrobial Resistant Enterotoxigenic Bacillus Species: A Materializing Epidemiological and Potential Severe Health Hazard

Although Bacillus cereus is of particular concern in food safety and public health, the role of other Bacillus species was overlooked. Therefore, we investigated the presence of eight enterotoxigenic genes, a hemolytic gene and phenotypic antibiotic resistance profiles of Bacillusspecies in retail meat samples. From 255 samples, 124 Bacillus isolates were recovered, 27 belonged to B. cereusand 97 were non-B. cereus species. Interestingly, the non-B. cereus isolates carried the virulence genes and exhibited phenotypic virulence characteristics as the B. cereus. However, correlation matrix analysis revealed the B. cereus group positively correlates with the presence of the genes hblA, hblC, and plc, and the detection of hemolysis (p \u3c 0.05), while the other Bacillus sp. groups are negatively correlated. Tests for antimicrobial resistance against ten antibiotics revealed extensive drug and multi-drug resistant isolates. Statistical analyses didn’t support a correlation of antibiotic resistance to tested virulence factors suggesting independence of these phenotypic markers and virulence genes. Of special interest was the isolation of Paenibacillus alvei and Geobacillus stearothermophilus from the imported meat samples being the first recorded. The isolation of non-B. cereus species carrying enterotoxigenic genes in meat within Egypt, suggests their impact on food safety and public health and should therefore not be minimised, posing an area that requires further research

Automated algorithm for CBCT-based dose calculations of prostate radiotherapy with bilateral hip prostheses

ABSTRACTOBJECTIVE:Cone beam CT (CBCT) images contain more scatter than a conventional CT image and therefore provide inaccurate Hounsfield units (HUs). Consequently, CBCT images cannot be used directly for radiotherapy dose calculation. The aim of this study is to enable dose calculations to be performed with the use of CBCT images taken during radiotherapy and evaluate the necessity of replanning.METHODS:A patient with prostate cancer with bilateral metallic prosthetic hip replacements was imaged using both CT and CBCT. The multilevel threshold (MLT) algorithm was used to categorize pixel values in the CBCT images into segments of homogeneous HU. The variation in HU with position in the CBCT images was taken into consideration. This segmentation method relies on the operator dividing the CBCT data into a set of volumes where the variation in the relationship between pixel values and HUs is small. An automated MLT algorithm was developed to reduce the operator time associated with the process. An intensity-modulated radiation therapy plan was generated from CT images of the patient. The plan was then copied to the segmented CBCT (sCBCT) data sets with identical settings, and the doses were recalculated and compared.RESULTS:Gamma evaluation showed that the percentage of points in the rectum with γ < 1 (3%/3 mm) were 98.7% and 97.7% in the sCBCT using MLT and the automated MLT algorithms, respectively. Compared with the planning CT (pCT) plan, the MLT algorithm showed −0.46% dose difference with 8 h operator time while the automated MLT algorithm showed −1.3%, which are both considered to be clinically acceptable, when using collapsed cone algorithm.CONCLUSION:The segmentation of CBCT images using the method in this study can be used for dose calculation. For a patient with prostate cancer with bilateral hip prostheses and the associated issues with CT imaging, the MLT algorithms achieved a sufficient dose calculation accuracy that is clinically acceptable. The automated MLT algorithm reduced the operator time associated with implementing the MLT algorithm to achieve clinically acceptable accuracy. This saved time makes the automated MLT algorithm superior and easier to implement in the clinical setting.ADVANCES IN KNOWLEDGE:The MLT algorithm has been extended to the complex example of a patient with bilateral hip prostheses, which with the introduction of automation is feasible for use in adaptive radiotherapy, as an alternative to obtaining a new pCT and reoutlining the structures

Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °C for 50 hours

Physical and chemical changes caused by oxidative stress in the spermatozoa membrane can reduce spermatozoa function and even lead to death. Cystamine (NH2-CH2-CH2-SH, β-mercaptoethylamine) is a natural substance that modulates the endocrine and metabolic status of animals. This substance has antioxidant and anti-apoptotic effects by inducing intracellular cysteine accumulation. Cystamine is used to treat many diseases despite its many side effects. Sheep semen is sensitive to the stressful condition of chilling storage, which restricts semen storage for artificial insemination in commercial herds. The effect of cystamine on spermatogenesis is not yet fully understood. The present study aimed to investigate the effect of cysteamine addition to the sheep sperm extender during cooling storage on semen quality parameters. Sperm samples were collected from six Edilbayevskaya rams (2 and 3 years old, 70-85 kg). The samples were diluted by extender and supplemented with different concentrations of cysteamine (0, 1, 2, 5, and 10 mM) and cooled to 4ºC for 50 h. Motility parameters, membrane integrity, viability, lipid peroxidation, and mitochondrial activity of cooled semen were evaluated at 0, 25, and 50 h of cooling storage. Although cysteamine failed to affect semen quality at start time (0 hrs), extender supplementation with cysteamine improved sperm total motility, progressive motility, and mitochondrial membrane potential during storage periods (P≤0.01). Moreover, using 1 and 2 mM cysteamine functionally and viably improved (P≤0.01) sperm membrane compared to other treatments. Antioxidant potential (AOP), lipid peroxidation (LPO), and total glutathione (tGSH) (except AOP at 50 h) were significantly different after semen storage at 4 °C. Therefore, levels of AOP and tGSH were significantly increased by using cysteamine. Cysteamine supplementation (1 and 2 mM cysteamine) leads to lower levels of LPO (p<0.01) at 0, 25, and 50 h. Therefore, finding and using the best concentrations of cysteamine in a cooling extender could be effective in saving sheep semen against damages of the cooling storage process

Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes

Background: miRNA profiling performed in myogenic cells and biopsies from skeletal muscles has previously identified miRNAs involved in myogenesis. Results: Here, we have performed miRNA transcriptome profiling in human affinity-purified CD56+ myoblasts induced to differentiate in vitro. In total, we have identified 60 miRNAs differentially expressed during myogenic differentiation. Many were not known for being differentially expressed during myogenic differentiation. Of these, 14 (miR-23b, miR-28, miR-98, miR-103, miR-107, miR-193a, miR-210, miR-324-5p, miR-324-3p, miR-331, miR-374, miR-432, miR-502, and miR-660) were upregulated and 6 (miR-31, miR-451, miR-452, miR-565, miR-594 and miR-659) were downregulated. mRNA transcriptome profiling performed in parallel resulted in identification of 6,616 genes differentially expressed during myogenic differentiation. Conclusions: This simultaneous miRNA/mRNA transcriptome profiling allowed us to predict with high accuracy target genes of myogenesis-related microRNAs and to deduce their functions