Urine patch detection using LiDAR and RPAS/UAV produced photogrammetry

In grazed dairy pastures, the largest N source for both nitrate (NO3-) leaching and nitrous oxide (N2O) emissions is urine-N excreted by the animals. Additional application of N on urine patches as fertilizer may increase these losses so adapting N-fertilisation in these areas is necessary. The objective of this study was to examine the use of a tractor mounted LiDAR (Light Detection and Ranging) system to accurately identify and quantify areas affect by excess N, such as urine and dung. To do so, a controlled experiment was designed in a paddock with no recent exposure to animals or N fertilisation. Synthetic urine was randomly applied within two 20m x 20m blocks and weekly LiDAR scans were taken for 5 weeks. LiDAR based contour maps of the pasture canopy were shown to accurately detect the asymmetric urine patches as well as calculate a percent area of urine based high N as early as one week after a simulated grazing event. Further, weekly flights were taken with a remotely piloted aircraft system (RPAS/UAV) to have aerial footage of the trial. Resulting mosaic of RGB and NIR images were used to create photogrammetric based contour maps. Both approaches (LiDAR and photogrammetry) show no significant difference in the identification and sizing of urine patch cluster

Developing young people's sense of self and place through sport

Previous research has recognized positive health implications, both physical and mental, as an outcome of participation in leisure pursuits. They provide opportunities for self-expression and stress reduction, as well as an environment in which people can socialize. Leisure activities, specifically sport activities, can play a significant role in young people's identity development. This paper explores the leisure activities in which young people in Adelaide, Australia participate. It examines the role of leisure activities in terms of young people's identity and feelings towards their hometown. This study consisted of semi-structured focus groups conducted with 24 senior high school students, followed by a survey resulting in 226 useable responses. Respondents were aged between 16 and 18 years of age. From the range of activities identified and explored, the results revealed sports activities to have the greatest impact on young people's lives. The results demonstrated that frequency of participation has a significant effect on young people's involvement levels and how they identify with the activity

Direct Observation of Self-Assembled Chain-Like Water Structures in a Nanoscopic Water Meniscus

Sawtooth-like oscillatory forces generated by water molecules confined between two oxidized silicon surfaces were observed using a cantilever-based optical interfacial force microscope when the two surfaces approached each other in ambient environments. The humidity-dependent oscillatory amplitude and periodicity were 3-12 nN and 3-4 water diameters, respectively. Half of each period was matched with a freely jointed chain model, possibly suggesting that the confined water behaved like a bundle of water chains. The analysis also indicated that water molecules self-assembled to form chain-like structures in a nanoscopic meniscus between two hydrophilic surfaces in air. From the friction force data measured simultaneously, the viscosity of the chain-like water was estimated to be between 108 and 1010 times greater than that of bulk water. The suggested chain-like structure resolves many unexplained properties of confined water at the nanometer scale, thus dramatically improving the understanding of a variety of water systems in nature

Catalyst Interface Engineering for Improved 2D Film Lift-Off and Transfer

The mechanisms by which chemical vapor deposited (CVD) graphene and hexagonal boron nitride (h-BN) films can be released from a growth catalyst, such as widely used copper (Cu) foil, are systematically explored as a basis for an improved lift-off transfer. We show how intercalation processes allow the local Cu oxidation at the interface followed by selective oxide dissolution, which gently releases the 2D material (2DM) film. Interfacial composition change and selective dissolution can thereby be achieved in a single step or split into two individual process steps. We demonstrate that this method is not only highly versatile but also yields graphene and h-BN films of high quality regarding surface contamination, layer coherence, defects, and electronic properties, without requiring additional post-transfer annealing. We highlight how such transfers rely on targeted corrosion at the catalyst interface and discuss this in context of the wider CVD growth and 2DM transfer literature, thereby fostering an improved general understanding of widely used transfer processes, which is essential to numerous other applications.We acknowledge funding from the ERC (InsituNANO, grant 279342). R.W. acknowledges an EPSRC Doctoral Training Award (EP/M506485/1). During this work, S.T. was supported in parts by a DFG research fellowship under grant TA 1122/1-1:1. J.A.A.-W. acknowledges a Research Fellowship from Churchill College, Cambridge. Z.A.V.V. acknowledges funding from ESPRC grant EP/L016087/1. P.B. and B.S.J. thank the Danish National Research Foundation Centre for Nanostructured graphene, DNRF103, and EU Horizon 2020 “Graphene Flagship” 696656. T.J.B. and P.R.W. acknowledge financial support from EU FP7-6040007 “GLADIATOR” and Innovation Fund Denmark Da-Gate 0603-005668B. P.R.K. acknowledges a Lindemann Trust Fellowship

Exclusion zones for variable rate nitrogen fertilisation in grazed dairy pasture systems in New Zealand

To assess the variability of total soil nitrogen (TN) on grazed and irrigated pastures, TN was quantified from spatially distinct “areas” within the paddock (irrigated and non-irrigated areas, around the gates, and around the troughs) on two dairy farms located in Canterbury, New Zealand. During soil sampling, each area was sub-divided and multiple soil samples were taken to ensure adequate spatial representation of each area. The results showed there were no differences in TN between the farms, but differences were detected between the paddocks (P< 0.001), largely due to the significant interaction between the areas (gates and troughs) in different paddocks (P< 0.001). The greatest variability in TN was around the gates, due to either much higher or lower TN near the entrance of the gates. The TN levels returned to concentrations that were similar to those in the surrounding pasture after 4 m distance from the gates. This study shows while TN concentrations are relatively consistent spatially within pastures, there is high variability in TN in proximity to some farm infrastructure, such as gates and troughs

Analysis of subcellular metabolite levels of potato tubers (Solanum tuberosum) displaying alterations in cellular or extracellular sucrose metabolism

The expression of a heterologous invertase in potato tubers (Solanum tuberosum) in either the cytosol or apoplast leads to a decrease in total sucrose content and to an increase in glucose. Depending on the targeting of the enzyme different changes in phenotype and metabolism of the tubers occur: the cytosolic invertase expressing tubers show an increase in the glycolytic flux, accumulation of amino acids and organic acids, and the appearance of novel disaccharides; however, these changes are not observed when the enzyme is expressed in the apoplast [Roessner et al. (2001). Plant Cell, 13, 11-29]. The analysis of these lines raised several questions concerning the regulation of compartmentation of metabolites in potato tubers. In the current study we addressed these questions by performing comparative subcellular metabolite profiling. We demonstrate that: (i) hexoses accumulate in the vacuole independently of their site of production, but that the cytosolic invertase expression led to a strong increase in the cytosolic glucose concentration and decrease in cytosolic sucrose, whereas these effects were more moderate in the apoplastic expressors; (ii) three out of four of the novel compounds found in the cytosolic overexpressors accumulate in the same compartment; (iii) despite changes in absolute cellular content the subcellular distribution of amino acids was invariant in the invertase overexpressing tubers. These results are discussed in the context of current models of the compartmentation of primary metabolism in heterotrophic plant tissues

Global mortality and readmission rates following COPD exacerbation-related hospitalisation: a meta-analysis of 65 945 individual patients

\ua9 2024, European Respiratory Society. All rights reserved.Background Exacerbations of COPD (ECOPD) have a major impact on patients and healthcare systems across the world. Precise estimates of the global burden of ECOPD on mortality and hospital readmission are needed to inform policy makers and aid preventive strategies to mitigate this burden. The aims of the present study were to explore global in-hospital mortality, post-discharge mortality and hospital readmission rates after ECOPD-related hospitalisation using an individual patient data meta-analysis (IPDMA) design. Methods A systematic review was performed identifying studies that reported in-hospital mortality, postdischarge mortality and hospital readmission rates following ECOPD-related hospitalisation. Data analyses were conducted using a one-stage random-effects meta-analysis model. This study was conducted and reported in accordance with the PRISMA-IPD statement. Results Data of 65 945 individual patients with COPD were analysed. The pooled in-hospital mortality rate was 6.2%, pooled 30-, 90- and 365-day post-discharge mortality rates were 1.8%, 5.5% and 10.9%, respectively, and pooled 30-, 90- and 365-day hospital readmission rates were 7.1%, 12.6% and 32.1%, respectively, with noticeable variability between studies and countries. Strongest predictors of mortality and hospital readmission included noninvasive mechanical ventilation and a history of two or more ECOPD-related hospitalisation

One more time with feeling: a rejoinder to Wood’s and Clair’s commentaries

In this rejoinder, we respond to the following claims regarding our integrated theory of domestic labor found in the commentaries by Julia T. Wood and Robin Patric Clair (2011, this issue): that we are establishing a ruler for the division of domestic; that our theory is biased toward the over-performer's rule or standard; that disagreements over domestic labor are primarily narrative constructions; and that our theory suggests a less than sanguine view of the potential for change in how men and women are socialized regarding the performance of domestic labor. In so doing, we argue that what Julia T. Wood describes as a “ruler” is not ours to create but rather is specific to each dyad and typically established by the partner with the lowest threshold level, be it a woman or a man. We also maintain that while a narrative approach provides a way to understand couples' interpretations of their experience, we believe a distinct value of our integrative theory is in helping to explain behavior and offer redress where such is needed. Finally, we argue that although our perspective regarding change may seem less than sanguine, that simply waiting for change to occur is of little benefit to those individuals and marriages/relationships suffering from lack of change in the here and now