Análisis de las propiedades psicométricas de la versión española del cuestionario de clima en el deporte

The psychometric properties of the Spanish version of the Sport Climate Questionnaire (S-SCQ) (SDT, Sport Climate Questionnaire, n.d.) were determined from a sample of 301 Spanish high-level competitive athletes (171 men and 130 women) from the Community of Valencia. The S-SCQ assesses athletes' perceptions of coach-provided autonomy support. The assumed uni-dimensional structure of both the long and short versions of the instrument was supported. Both versions also exhibited high reliability (internal consistency). Support for the convergent validity of the scale(s) was also obtained as the measure of coach autonomy support significantly related to the different types of motivation regulations (as assessed via the Sport Motivation Scale; Pelletier et al., 1995), in accordance with theoretical predictions

Examination of the psychometric properties of the spanish version of the Approach to Coping in Sport questionaire

This study examined the psychometric properties of a Spanish version of the Approach to Coping in Sport Questionnaire and potential cultural differences in coping strategies between Spanish, Korean and US athletes. A total of 190 Spanish athletes (Male, N =152, Female, N = 38, M age = 21.2 + 4.4 years) from various sports participated in the study. After specifying the frequency of the experience of psychological difficulties during competition, the athletes indicated how often they used each of the coping strategies targeted in the ACSQ-Spanish and they also completed the Athletic Coping Skills Inventory-28. The results of CFA provided support for the factorial validity of the ACSQ-Spanish and preliminary evidence regarding concurrent and convergent validity was garnered. Differences in the use of different coping strategies between the Spanish, Korean, and US athlete groups emerged.En este estudio se analizan las propiedades psicométricas de la versión Española del ACSP (Approach to Coping in Sport Questionnaire) y las potenciales diferencias culturales existentes entre los deportistas españoles, coreanos y americanos en las estrategias de afrontamiento. 190 deportistas españoles (Hombres, N = 152; Mujeres, N = 38; M edad = 21.2 + 4.4 años) de varios deportes participaron en este estudio. Después de especificar la frecuencia de dificultades psicológicas que tenían durante la competición, los deportistas indicaban con que frecuencia utilizaban cada una de las estrategias de afrontamiento que figuraban en ACSP-Español y también rellenaron el Athletic Coping Skills Inventory 28. Los resultados del análisis factorial confirmatorio ofrecieron apoyo para la validez factorial del ACSQ-español. También se obtuvo evidencia preliminar sobre la validez concurrente y convergente del cuestionario. Se obtuvieron diferencias en las estrategias de afrontamiento utilizadas entre los deportistas españoles, coreanos y americanos

Escala Mexicana de Satisfacción de las Necesidades Psicológicas Básicas en Educación Física

El objetivo del presente trabajo consistió en traducir al español hablado en México y examinar las propiedades psicométricas de la versión mexicana de la Escala de Satisfacción de las Necesidades Psicológicas Básicas en la Educación Física (ESNPB-EF) en estudiantes de secundaria. En el estudio 1 se realizó la traducción y adaptación de la escala, se examinó su estructura factorial, así como su fiabilidad en una muestra de 293 estudiantes (Medad = 13.68 años; DT = 1.01); y en el segundo estudio se evaluó la fiabilidad de la escala, se examinó su estructura factorial, y se analizó la invarianza del instrumento a través del género en una muestra de 734 estudiantes (Medad = 13.7 años; DT = 1.09). Los resultados revelaron que el modelo de tres factores era aceptable (competencia, autonomía y relaciones). Los análisis apoyaron la replicabilidad de la estructura factorial a través del género, así como la consistencia interna de la escala (α > .77). En suma, la versión mexicana de la ESNPB-EF representa una adaptación válida y fiable para medir la satisfacción de las necesidades psicológicas básicas de los estudiantes de educación física en México.The aim of this study was to translate into Spanish spoken in Mexico and examine the psychometric properties of the Mexican version of the basic psychological needs satisfaction in physical education scale (ESNPB-EF) in Mexican high school students. In the Study 1, translation and adaptation of the scale were done, factorial structure and reliability were tested in a sample of 293 students (Mage = 13.68 years, SD = 1.01); and in the second study the factorial structure and the invariance across gender were examined in a sample of 734 students (Mage = 13.7 years, SD = 1.09). The results revealed that a three factors structure (competence, autonomy and relatedness) was acceptable. Results supported the invariance of the ESNPBEF across gender and provided support for the internal consistency of the scale (α > .77). Overall, the Mexican version of the ESNPB-EF represents a valid and reliable adaptation of the instrument, which can be confidently used to measure satisfaction of the basic psychological needs in physical education students.O objetivo deste estudo foi traduzir para o espanhol falado no México e examinar as propriedades psicométricas da versão mexicana da Escala de Satisfação das Necessidades Psicológicas Básicas em Educação Física (ESNPB-EF) em estudantes do ensino médio. No Estudo 1, a tradução e a adaptação da escala foi realizada, foi examinada a estrutura fatorial e a fiabilidade de uma amostra de 293 estudantes (Midade = 13,68 anos, DP =1,01); e no segundo estudo, a fiabilidade da escala foi avaliada, a estrutura fatorial foi examinada, e a invariância do instrumento foi analisada através de gênero em uma amostra de 734 estudantes (Midade = 13,7 anos, DP = 1,09). Os resultados revelaram que o modelo de três fatores foi aceitável (competência, autonomia e relações). As análises suportaram a replicabilidade da estrutura fatorial atraves do gênero, mesmo assim como a consistência interna da escala (α > 0,77). Em suma, a versão mexicana do ESNPB-EF representa uma adaptação válida e confiável para medir a satisfação das necessidades psicológicas básicas de estudantes de educação física no México

Actividad antibacteriana del extracto etanólico de las hojas de jungia rugosa less (matico serrano) sobre cepas de staphylococcus aureus

El presente trabajo tuvo como objetivo determinar si el extracto etanólico de las hojas de Jungia rugosa L. posee efecto antibacteriano sobre las cepas de Staphylococcus aureus ATCC 6538. Metodología el estudio fue de tipo experimental, diseño experimental mixto, transversal, in vitro.The objective of this work was to determine if the ethanolic extract of the leaves of Jungia rugosa L. has an antibacterial effect on the strains of Staphylococcus aureus ATCC 6538. Methodology The study was of experimental type, mixed experimental design, transverse, in vitro

Coinoculation of soybean plants with Bradyrhizobium japonicum and Trichoderma harzianum: Coexistence of both microbes and relief of nitrate inhibition of nodulation

Coinoculation of plants with mixtures of beneficial microbes sometimes produces synergistic effects. In this study, the effect of soybean coinoculation with the N2-fixing Bradyrhizobium japonicum E109 and the biocontrol fungus Trichoderma harzianum Th5cc was analyzed. Nodulation by E109 was not hampered by Th5cc, which antagonized five out of seven soybean pathogens tested. Furthermore, Th5cc relieved nitrate-inhibition of nodulation, enabling the formation of nodules containing infected cells with bacteroids in the presence of the otherwise inhibitory 10 mM KNO3. Th5cc released micromolar amounts of auxin, and addition of 11 mM indoleacetic acid to soybean plants inoculated with E109 in the absence of Th5cc also induced nodulation in the presence of 10 mM KNO3. Thus, Th5cc may release auxins into the soybean rhizosphere, which hormones might participate in overcoming the nitrate-inhibition of nodulation. Our results suggest that soybean plants coinoculated with these microorganisms might benefit from biocontrol while contributing to soil-nitrogen preservation.Fil: Iturralde, Esteban Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Stocco, Marina Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaFil: Faura, Andrés. Rizobacter Argentina S.A.; ArgentinaFil: Monaco, Cecilia Ines. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Cordo, Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Pérez Giménez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Laboratorio de Genética; Argentin

Genomic Analysis of Molecular Bacterial Mechanisms of Resistance to Phage Infection

[Abstract] To optimize phage therapy, we need to understand how bacteria evolve against phage attacks. One of the main problems of phage therapy is the appearance of bacterial resistance variants. The use of genomics to track antimicrobial resistance is increasingly developed and used in clinical laboratories. For that reason, it is important to consider, in an emerging future with phage therapy, to detect and avoid phage-resistant strains that can be overcome by the analysis of metadata provided by whole-genome sequencing. Here, we identified genes associated with phage resistance in 18 Acinetobacter baumannii clinical strains belonging to the ST-2 clonal complex during a decade (Ab2000 vs. 2010): 9 from 2000 to 9 from 2010. The presence of genes putatively associated with phage resistance was detected. Genes detected were associated with an abortive infection system, restriction–modification system, genes predicted to be associated with defense systems but with unknown function, and CRISPR-Cas system. Between 118 and 171 genes were found in the 18 clinical strains. On average, 26% of these genes were detected inside genomic islands in the 2000 strains and 32% in the 2010 strains. Furthermore, 38 potential CRISPR arrays in 17 of 18 of the strains were found, as well as 705 proteins associated with CRISPR-Cas systems. A moderately higher presence of these genes in the strains of 2010 in comparison with those of 2000 was found, especially those related to the restriction–modification system and CRISPR-Cas system. The presence of these genes in genomic islands at a higher rate in the strains of 2010 compared with those of 2000 was also detected. Whole-genome sequencing and bioinformatics could be powerful tools to avoid drawbacks when a personalized therapy is applied. In this study, it allows us to take care of the phage resistance in A. baumannii clinical strains to prevent a failure in possible phage therapy.This study was funded by grants PI16/01163 and PI19/00878 awarded to MT within the State Plan for R + D + I 2013–2016 (National Plan for Scientific Research, Technological Development and Innovation 2008–2011) and co-financed by the ISCIII-Deputy General Directorate of evaluation and Promotion of Research-European Regional Development Fund “A way of Making Europe” and Instituto de Salud Carlos III FEDER. MT was financially supported by the Miguel Servet Research Programme (SERGAS and ISCIII

CRISPR-Cas, a Revolution in the Treatment and Study of ESKAPE Infections: Pre-Clinical Studies

This article belongs to the Special Issue Non-antimicrobial Agents as Adjuvants against Bacterial Infections[Abstract] One of the biggest threats we face globally is the emergence of antimicrobial-resistant (AMR) bacteria, which runs in parallel with the lack in the development of new antimicrobials. Among these AMR bacteria pathogens belonging to the ESKAPE group can be highlighted (Enterococcus spp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.) due to their profile of drug resistance and virulence. Therefore, innovative lines of treatment must be developed for these bacteria. In this review, we summarize the different strategies for the treatment and study of molecular mechanisms of AMR in the ESKAPE pathogens based on the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins’ technologies: loss of plasmid or cellular viability, random mutation or gene deletion as well directed mutations that lead to a gene’s loss of function.This study was funded by grants PI16/01163 and PI19/00878 awarded to M. Tomás within the State Plan for R+D+I 2013–2016 (National Plan for Scientific Research, Technological Development and Innovation 2008–2011) and cofinanced by the ISCIII-Deputy General Directorate for Evaluation and Promotion of Research-European Regional Development Fund “A way of Making Europe” and Instituto de Salud Carlos III FEDER, Spanish Network for the Research in Infectious Diseases (REIPI, RD16/0016/0006) and by the Study Group on Mechanisms of Action and Resistance to Antimicrobials, GEMARA (SEIMC, http://www.seimc.org/, Accessed on 21 June 2021). I. Bleriot was financially supported by pFIS program (ISCIII, FI20/00302). O. Pacios and M. López was financially supported by a grant IN606A-2020/035 and IN606B-2018/008, respectively, (GAIN, Xunta de Galicia), and M. Gonzalez-Bardanca was financially supported by the Rio Hortega program (ISCIII, CM20/00198)Xunta de Galicia; IN606A-2020/035Xunta de Galicia; IN606B-2018/00

EXPERIENCIA EDUCATIVA : TALLER DE RECURSOS NATURALES II

El Taller de Recursos Naturales II (TRN II) se desarrolla durante el 2do cuatrimestre del segundo año de Agronomía. La información obtenida por los alumnos es utilizada en el Taller de Producción Vegetal en el 4to Año. De acuerdo a la estructura y lineamientos del Plan de Estudios, el TRN II integra actividades de las asignaturas dictadas en ese cuatrimestre como: Propiedades Edáficas y Fertilidad, Fisiología Vegetal, Genética Básica y Aplicada y Agrometeorología, además de Ecología y Zoología Agrícola, asignaturas de tercero y cuarto año respectivamente. Tiene como objetivo conducir al alumno hacia una compresión global de los factores que afectan el crecimiento de las plantas con el fin de mantener o aumentar la producción, dentro del marco de una agricultura sustentable. Finaliza con una puesta en común donde se interpretan los resultados en términos ecofisiológicos y de interacción genotipo-ambiente, utilizando información obtenida de las demás asignaturas. Así, otorga a los futuros profesionales la capacidad de analizar los conocimientos adquiridos a campo y en el laboratorio, a la luz de los factores investigados durante el desarrollo del Taller

Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13

[Abstract] Klebsiella pneumoniae is a human pathogen that worsens the prognosis of many immunocompromised patients. Here, we annotated and compared the genomes of two lytic phages that infect clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically characterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 ± 0.61 PFU/infected cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM-VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM-VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylogenetic study performed with other Tevenvirinae phages showed a close common ancestor. However, there were 21 coding sequences which differed. Interestingly, the main differences were that vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nucleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading to different three-dimensional protein predictions. Both phages differed significantly in their host range. These viruses may be useful in the development of alternative therapies to antibiotics or as a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant (MDR) pathogens.This study was funded by grants PI19/00878 awarded to M. Tomás within the State Plan for R+D+I 2013-2016 (National Plan for Scientific Research, Technological Development and Innovation 2008–2011) and co-financed by the ISCIII-Deputy General Directorate for Evaluation and Promotion of Research—European Regional Development Fund “A way of Making Europe” and Instituto de Salud Carlos III FEDER, Spanish Network for the Research in Infectious Diseases (REIPI, RD16/0016/0001, RD16/0016/0006 and RD16/CIII/0004/0002) and by the Study Group on Mechanisms of Action and Resistance to Antimicrobials, GEMARA (SEIMC, http://www.seimc.org/ accessed on 1 February 2021) and project PID2020-112835RA-I00 funded by MCIN/AEI /10.13039/501100011033, and project SEJIGENT/2021/014 funded by Conselleria d’Innovació, Universitats, Ciència i Societat Digital (Generalitat Valenciana). M. Tomás was financially supported by the Miguel Servet Research Programme (SERGAS and ISCIII). O. Pacios, L. Fernández-García and M. López were financially supported by the grants IN606A-2020/035, IN606B-2021/013 and IN606B-2018/008, respectively (GAIN, Xunta de Galicia). I. Bleriot was financially supported by pFIS program (ISCIII, FI20/00302). P. Domingo-Calap was financially supported by a Ramón y Cajal contract RYC2019-028015-I funded by MCIN/AEI /10.13039/501100011033, ESF Invest in your futureGeneralitat Valenciana; SEJIGENT/2021/014Xunta de Galicia; IN606A-2020/035Xunta de Galicia; IN606B-2021/013Xunta de Galicia; IN606B-2018/00