Neutralino spectrum in top-down models of UHECR

We calculate the cosmic ray spectrum of ultra high energy neutralinos that one should expect provided that the observed ultra high energy cosmic rays are produced by the decay of superheavy particles X, M_X>10^{12} GeV, in supersymmetric models. Our calculation uses an extended DGLAP formalism. Forthcoming cosmic ray observatories should be able to detect these neutralinos.Comment: 10 pages, revtex, 3 eps figures. Difference between the present work and Montecarlo simulations clarifie

Gravitinos from Gravitational Collapse

We reanalyse the limits on the gravitino mass $m_{3/2}$ in superlight gravitino scenarios derived from arguments on energy-loss during gravitational collapse. We conclude that the mass range $10^{-6}eV\leq m_{3/2}\leq2.3\times10^{-5}eV$ is excluded by SN1987A data. In terms of the scale of supersymmetry breaking $\Lambda$, the range $70GeV\leq\Lambda \leq 300GeV$ is not allowed.Comment: 6 pages, latex, no figures. Numerical typo correcte

Gamma Rays from SN1987A due to Pseudoscalar Conversion

A light pseudoscalar coupled to two photons would be copiously emitted by the core of a supernova. Part of this flux would be converted to $\gamma-$rays by the galactic magnetic field. Measurements on the SN1987A $\gamma-$ray flux by the Gamma-Ray Spectrometer on the Solar Maximum Mission satellite already imply a bound on the coupling $g < 3 \times 10^{-12}$ GeV$^{-1}$. The improved generation of satellite-borne detectors, like EGRET or the project GLAST, could be able to detect a pseudoscalar-to-photon signal from a nearby supernova, for allowed values of $g$.Comment: 8 pages, no figure

On a Light Spinless Particle Coupled to Photons

A pseudoscalar or scalar particle $\phi$ that couples to two photons but not to leptons, quarks and nucleons would have effects in most of the experiments searching for axions, since these are based on the $a \gamma \gamma$ coupling. We examine the laboratory, astrophysical and cosmological constraints on $\phi$ and study whether it may constitute a substantial part of the dark matter. We also generalize the $\phi$ interactions to possess $SU(2) \times U(1)$ gauge invariance, and analyze the phenomenological implications.Comment: LaTex, 20p., 6 figures. Changes in sections 4, 5 and figure 2, our bounds are now more stringent. To be published in Physical Review

Nanocellulose/bioactive glass cryogels as scaffolds for bone regeneration

A major challenge exists in the preparation of scaffolds for bone regeneration, namely, achieving simultaneously bioactivity, biocompatibility, mechanical performance and simple manufacturing. Here, cellulose nanofibrils (CNF) are introduced for the preparation of scaffolds taking advantage of their biocompatibility and ability to form strong 3D porous networks from aqueous suspensions. CNF are made bioactive for bone formation through a simple and scalable strategy that achieves highly interconnected 3D networks. The resultant materials optimally combine morphological and mechanical features and facilitate hydroxyapatite formation while releasing essential ions for in vivo bone repair. The porosity and roughness of the scaffolds favor several cell functions while the ions act in the expression of genes associated with cell differentiation. Ion release is found critical to enhance the production of the bone morphogenetic protein 2 (BMP-2) from cells within the fractured area, thus accelerating the in vivo bone repair. Systemic biocompatibility indicates no negative effects on vital organs such as the liver and kidneys. The results pave the way towards a facile preparation of advanced, high performance CNF-based scaffolds for bone tissue engineering

Free amino acid profile of Bubalus bubalis L. meat from the Campania region

ABSTRACT In this study, we determined the amount of carnosine and anserine in water buffalo meat without hanging treatment and the free amino acid profile by using amino acid analyser with post-column ninhydrin derivatization procedure. The main free amino acids present in samples were glutamic acid (>60 mg/100 g), followed by alanine, glycine, and arginine. Other protein amino acids were detected in minor amounts (less than 2 mg/100 g). Among the non-protein amine-containing compounds, taurine and urea were the most abundant. The analysis showed that 50% of the total free amino acids was represented by dipeptides carnosine (average ~130.3 mg/100 g) and anserine (average ~17.9 mg/100 g). Thus, this study for the first time reports the free amino acids profile of water buffalo meat and the content of carnosine and anserine, potentially involved in the darkening meat process and their ratio, that could be used to estimate the water buffalo meat portion in mixed meat products

Discovering the Potential of Dental Pulp Stem Cells for Corneal Endothelial Cell Production: A Proof of Concept

Failure of corneal endothelium cell monolayer is the main cause leading to corneal transplantation. Autologous cell-based therapies are required to reconstruct in vitro the cell monolayer. Several strategies have been proposed using embryonic stem cells and induced pluripotent stem cells, although their use has ethical issues as well as limited clinical applications. For this purpose, we propose the use of dental pulp stem cells isolated from the third molars to form the corneal endothelium cell monolayer. We hypothesize that using dental pulp stem cells that share an embryological origin with corneal endothelial cells, as they both arise from the neural crest, may allow a direct differentiation process avoiding the use of reprogramming techniques, such as induced pluripotent stem cells. In this work, we report a two-step differentiation protocol, where dental pulp stem cells are derived into neural crest stem-like cells and, then, into corneal endothelial-like cells. Initially, for the first-step we used an adhesion culture and compared two initial cell sources: a direct formation from dental pulp stem cells with the differentiation from induced pluripotent stem cells. Results showed significantly higher levels of early stage marker AP2 for the dental pulp stem cells compared to induced pluripotent stem cells. In order to provide a better environment for neural crest stem cells generation, we performed a suspension method, which induced the formation of neurospheres. Results showed that neurosphere formation obtained the peak of neural crest stem cell markers expression after 4 days, showing overexpression of AP2, Nestin, and p75 markers, confirming the formation of neural crest stem-like cells. Furthermore, pluripotent markers Oct4, Nanog, and Sox2 were as well-upregulated in suspension culture. Neurospheres were then directly cultured in corneal endothelial conditioned medium for the second differentiation into corneal endothelial-like cells. Results showed the conversion of dental pulp stem cells into polygonal-like cells expressing higher levels of ZO-1, ATP1A1, COL4A2, and COL8A2 markers, providing a proof of the conversion into corneal endothelial-like cells. Therefore, our findings demonstrate that patient-derived dental pulp stem cells may represent an autologous cell source for corneal endothelial therapies that avoids actual transplantation limitations as well as reprogramming techniques