238 research outputs found
Publisher Correction: Symbiodinium genomes reveal adaptive evolution of functions related to coral-dinoflagellate symbiosis
Symbiodinium genomes reveal adaptive evolution of functions related to coral-dinoflagellate symbiosis
Symbiosis between dinoflagellates of the genus Symbiodinium and reef-building corals forms the trophic foundation of the world’s coral reef ecosystems. Here we present the first draft genome of Symbiodinium goreaui (Clade C, type C1: 1.03 Gbp), one of the most ubiquitous endosymbionts associated with corals, and an improved draft genome of Symbiodinium kawagutii (Clade F, strain CS-156: 1.05 Gbp) to further elucidate genomic signatures of this symbiosis. Comparative analysis of four available Symbiodinium genomes against other dinoflagellate genomes led to the identification of 2460 nuclear gene families (containing 5% of Symbiodinium genes) that show evidence of positive selection, including genes involved in photosynthesis, transmembrane ion transport, synthesis and modification of amino acids and glycoproteins, and stress response. Further, we identify extensive sets of genes for meiosis and response to light stress. These draft genomes provide a foundational resource for advancing our understanding of Symbiodinium biology and the coral-algal symbiosis.H.L.
was supported by an Australian Research Council grant (DP150101875) awarded to M.A.
R. and C.X.C. T.G.S. is supported by an Australian Government Research Training
Program Scholarship. R.A.G.-P. is supported by an International Postgraduate Research
Scholarship and a University of Queensland Centenary Scholarship. This project was
supported by the computational resources of the Australian National Computational
Infrastructure (NCI) National Facility systems through the NCI Merit Allocation Scheme
(Project d85) awarded to M.A.R. and C.X.C. The data used in this project were funded by
the Great Barrier Reef Foundation’s Resilient Coral Reefs Successfully Adapting to Climate Change research and development program in collaboration with the Australian
Government, Bioplatforms Australia through the National Collaborative Research
Infrastructure Strategy (NCRIS), Rio Tinto and a family foundation. The authors also
acknowledge the work done by the Reef Future Genomics (ReFuGe) 2020 Consortium.
Access to data generated by the consortium can be accessed via reefgenomics.org. In
memory of S.F., our friend and colleague who is sorely missed
Association of a Bovine Prion Gene Haplotype with Atypical BSE
Background: Atypical bovine spongiform encephalopathies (BSEs) are recently recognized prion diseases of cattle. Atypical BSEs are rare; approximately 30 cases have been identified worldwide. We tested prion gene (PRNP) haplotypes for an association with atypical BSE.
Methodology/Principle Findings: Haplotype tagging polymorphisms that characterize PRNP haplotypes from the promoter region through the three prime untranslated region of exon 3 (25.2 kb) were used to determine PRNP haplotypes of six available atypical BSE cases from Canada, France and the United States. One or two copies of a distinct PRNP haplotype were identified in five of the six cases (p = 1.36×10-4, two-tailed Fisher’s exact test; CI95% 0.263–0.901, difference between proportions). The haplotype spans a portion of PRNP that includes part of intron 2, the entire coding region of exon 3 and part of the three prime untranslated region of exon 3 (13 kb).
Conclusions/Significance: This result suggests that a genetic determinant in or near PRNP may influence susceptibility of cattle to atypical BSE
The Most Metal-Poor Stars. II. Chemical Abundances of 190 Metal-Poor Stars Including 10 New Stars With [Fe/H] < -3.5
We present a homogeneous chemical abundance analysis of 16 elements in 190
metal-poor Galactic halo stars (38 program and 152 literature objects). The
sample includes 171 stars with [Fe/H] < -2.5, of which 86 are extremely metal
poor, [Fe/H] < -3.0. Our program stars include ten new objects with [Fe/H] <
-3.5. We identify a sample of "normal" metal-poor stars and measure the trends
between [X/Fe] and [Fe/H], as well as the dispersion about the mean trend for
this sample. Using this mean trend, we identify objects that are chemically
peculiar relative to "normal" stars at the same metallicity. These chemically
unusual stars include CEMP-no objects, one star with high [Si/Fe], another with
high [Ba/Sr], and one with unusually low [X/Fe] for all elements heavier than
Na. The Sr and Ba abundances indicate that there may be two nucleosynthetic
processes at lowest metallicity that are distinct from the main r-process.
Finally, for many elements, we find a significant trend between [X/Fe] versus
Teff which likely reflects non-LTE and/or 3D effects. Such trends demonstrate
that care must be exercised when using abundance measurements in metal-poor
stars to constrain chemical evolution and/or nucleosynthesis predictions.Comment: Accepted for publication in Ap
Recommended from our members
Implementation of U.K. Earth system models for CMIP6
We describe the scientific and technical implementation of two models for a core set of
experiments contributing to the sixth phase of the Coupled Model Intercomparison Project (CMIP6).
The models used are the physical atmosphere-land-ocean-sea ice model HadGEM3-GC3.1 and the
Earth system model UKESM1 which adds a carbon-nitrogen cycle and atmospheric chemistry to
HadGEM3-GC3.1. The model results are constrained by the external boundary conditions (forcing data)
and initial conditions.We outline the scientific rationale and assumptions made in specifying these.
Notable details of the implementation include an ozone redistribution scheme for prescribed ozone
simulations (HadGEM3-GC3.1) to avoid inconsistencies with the model's thermal tropopause, and land use
change in dynamic vegetation simulations (UKESM1) whose influence will be subject to potential biases in
the simulation of background natural vegetation.We discuss the implications of these decisions for
interpretation of the simulation results. These simulations are expensive in terms of human and CPU
resources and will underpin many further experiments; we describe some of the technical steps taken to
ensure their scientific robustness and reproducibility
Recommended from our members
Making Connections - Envisioning Springfield\u27s North End
This work explores a service learning strategy in the context of the senior Urban Design Studio taught in the Department of Landscape Architecture and Regional Planning at the University of Massachusetts Amherst. The primary goal of this project is to stimulate a conversation in the neighborhoods of the North End, to develop green design strategies, to improve services and businesses for residents and the employees of local businesses, and to foster cultural engagement and interaction in the North End that will enhance the vibrancy, resilience, and quality of life of this urban community. Making connections - Envisioning Springfield\u27s North End proposes improved connectivity in a physical, cultural, and social sense will be key to attaining these goals and to engaging and synergizing individuals and community groups in the North End - residents, businesses, schools, churches, employers, and employees. Six sustainable learning and planning principles have emerged from this studio:
1. Input and interaction – Visioning workshops connect campus and community
2. Community-building art - Expression of place and people
3. Healthy living - Urban agriculture and education
4. Urban greenways – Abandoned railways and urban rivers and streams
5. Green infrastructure - Green streets as networks and structural framework
6. Sustainable urban form – Mixed use and pedestrian friendly neighborhood
Abnormal Glycosphingolipid Mannosylation Triggers Salicylic Acid–Mediated Responses in Arabidopsis
Novel computational methods for increasing PCR primer design effectiveness in directed sequencing
<p>Abstract</p> <p>Background</p> <p>Polymerase chain reaction (PCR) is used in directed sequencing for the discovery of novel polymorphisms. As the first step in PCR directed sequencing, effective PCR primer design is crucial for obtaining high-quality sequence data for target regions. Since current computational primer design tools are not fully tuned with stable underlying laboratory protocols, researchers may still be forced to iteratively optimize protocols for failed amplifications after the primers have been ordered. Furthermore, potentially identifiable factors which contribute to PCR failures have yet to be elucidated. This inefficient approach to primer design is further intensified in a high-throughput laboratory, where hundreds of genes may be targeted in one experiment.</p> <p>Results</p> <p>We have developed a fully integrated computational PCR primer design pipeline that plays a key role in our high-throughput directed sequencing pipeline. Investigators may specify target regions defined through a rich set of descriptors, such as Ensembl accessions and arbitrary genomic coordinates. Primer pairs are then selected computationally to produce a minimal amplicon set capable of tiling across the specified target regions. As part of the tiling process, primer pairs are computationally screened to meet the criteria for success with one of two PCR amplification protocols. In the process of improving our sequencing success rate, which currently exceeds 95% for exons, we have discovered novel and accurate computational methods capable of identifying primers that may lead to PCR failures. We reveal the laboratory protocols and their associated, empirically determined computational parameters, as well as describe the novel computational methods which may benefit others in future primer design research.</p> <p>Conclusion</p> <p>The high-throughput PCR primer design pipeline has been very successful in providing the basis for high-quality directed sequencing results and for minimizing costs associated with labor and reprocessing. The modular architecture of the primer design software has made it possible to readily integrate additional primer critique tests based on iterative feedback from the laboratory. As a result, the primer design software, coupled with the laboratory protocols, serves as a powerful tool for low and high-throughput primer design to enable successful directed sequencing.</p
Genome sequence of an Australian kangaroo, Macropus eugenii, provides insight into the evolution of mammalian reproduction and development.
BACKGROUND: We present the genome sequence of the tammar wallaby, Macropus eugenii, which is a member of the kangaroo family and the first representative of the iconic hopping mammals that symbolize Australia to be sequenced. The tammar has many unusual biological characteristics, including the longest period of embryonic diapause of any mammal, extremely synchronized seasonal breeding and prolonged and sophisticated lactation within a well-defined pouch. Like other marsupials, it gives birth to highly altricial young, and has a small number of very large chromosomes, making it a valuable model for genomics, reproduction and development. RESULTS: The genome has been sequenced to 2 × coverage using Sanger sequencing, enhanced with additional next generation sequencing and the integration of extensive physical and linkage maps to build the genome assembly. We also sequenced the tammar transcriptome across many tissues and developmental time points. Our analyses of these data shed light on mammalian reproduction, development and genome evolution: there is innovation in reproductive and lactational genes, rapid evolution of germ cell genes, and incomplete, locus-specific X inactivation. We also observe novel retrotransposons and a highly rearranged major histocompatibility complex, with many class I genes located outside the complex. Novel microRNAs in the tammar HOX clusters uncover new potential mammalian HOX regulatory elements. CONCLUSIONS: Analyses of these resources enhance our understanding of marsupial gene evolution, identify marsupial-specific conserved non-coding elements and critical genes across a range of biological systems, including reproduction, development and immunity, and provide new insight into marsupial and mammalian biology and genome evolution
- …