16 research outputs found

    Doing the Job: The 1964 Desgregation of the Florida Army National Guard

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    The American civil rights movement usually brings to mind cities such as Montgomery, Birmingham, Memphis and Washington, D.C. In Florida, however, one of the most significant events of the civil rights era occurred without fanfare in the small, west coast town of Palmetto. It was here in 1964 that the color barrier was broken in one of the state\u27s largest whites-only organizations, The Florida Army National Guard. For the first time in post-colonial history, African-Americans were enlisted into the state\u27s military. Prior to American control, African Americans did play a military role in Florida. State guard officials claim that the first black militia unit in North America was formed under Spanish rule in 1683. The company of free men of color was commanded by a free black, Francisco Menendez until at least 1742. Free black Floridians also served the British during the American War for Independence and Spain again during the Second Period of Spanish Occupation. Historian Robert Hawk notes that while black units enrolled in the state militia from 1865 to 1901, they received no state support and no encouragement to become active units.

    Assignment of Streptococcus agalactiae isolates to clonal complexes using a small set of single nucleotide polymorphisms

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    <p>Abstract</p> <p>Background</p> <p><it>Streptococcus agalactiae </it>(Group B Streptococcus (GBS)) is an important human pathogen, particularly of newborns. Emerging evidence for a relationship between genotype and virulence has accentuated the need for efficient and well-defined typing methods. The objective of this study was to develop a single nucleotide polymorphism (SNP) based method for assigning GBS isolates to multilocus sequence typing (MLST)-defined clonal complexes.</p> <p>Results</p> <p>It was found that a SNP set derived from the MLST database on the basis of maximisation of Simpsons Index of Diversity provided poor resolution and did not define groups concordant with the population structure as defined by eBURST analysis of the MLST database. This was interpreted as being a consequence of low diversity and high frequency horizontal gene transfer. Accordingly, a different approach to SNP identification was developed. This entailed use of the "Not-N" bioinformatic algorithm that identifies SNPs diagnostic for groups of known sequence variants, together with an empirical process of SNP testing. This yielded a four member SNP set that divides GBS into 10 groups that are concordant with the population structure. A fifth SNP was identified that increased the sensitivity for the clinically significant clonal complex 17 to 100%. Kinetic PCR methods for the interrogation of these SNPs were developed, and used to genotype 116 well characterized isolates.</p> <p>Conclusion</p> <p>A five SNP method for dividing GBS into biologically valid groups has been developed. These SNPs are ideal for high throughput surveillance activities, and combining with more rapidly evolving loci when additional resolution is required.</p

    Hectospec, the MMT's 300 Optical Fiber-Fed Spectrograph

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    The Hectospec is a 300 optical fiber fed spectrograph commissioned at the MMT in the spring of 2004. A pair of high-speed six-axis robots move the 300 fiber buttons between observing configurations within ~300 s and to an accuracy ~25 microns. The optical fibers run for 26 m between the MMT's focal surface and the bench spectrograph operating at R~1000-2000. Another high dispersion bench spectrograph offering R~5,000, Hectochelle, is also available. The system throughput, including all losses in the telescope optics, fibers, and spectrograph peaks at ~10% at the grating blaze in 1" FWHM seeing. Correcting for aperture losses at the 1.5" diameter fiber entrance aperture, the system throughput peaks at \sim17%. Hectospec has proven to be a workhorse instrument at the MMT. Hectospec and Hectochelle together were scheduled for 1/3 of the available nights since its commissioning. Hectospec has returned \~60,000 reduced spectra for 16 scientific programs during its first year of operation.Comment: 68 pages, 28 figures, to appear in December 2005 PAS

    Laser produced electromagnetic pulses : Generation, detection and mitigation

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    This paper provides an up-to-date review of the problems related to the generation, detection and mitigation of strong electromagnetic pulses created in the interaction of high-power, high-energy laser pulses with different types of solid targets. It includes new experimental data obtained independently at several international laboratories. The mechanisms of electromagnetic field generation are analyzed and considered as a function of the intensity and the spectral range of emissions they produce. The major emphasis is put on the gHz frequency domain, which is the most damaging for electronics and may have important applications. The physics of electromagnetic emissions in other spectral domains, in particular THz and MHz, is also discussed. The theoretical models and numerical simulations are compared with the results of experimental measurements, with special attention to the methodology of measurements and complementary diagnostics. Understanding the underlying physical processes is the basis for developing techniques to mitigate the electromagnetic threat and to harness electromagnetic emissions, which may have promising applications
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