LITERACY AND LEARNING ACROSS PHYSICAL AND DIGITAL SPACES: A CASE STUDY IN A BLENDED PRIMARY CLASSROOM

In light of technological innovations, schools are increasingly adopting digital tools and promoting online spaces for learning. Consequently, the shape of teaching and learning is shifting beyond the physical classroom. Drawing on sociocultural theory, distributed cognition and a networked learning framework, this case study explores how a blended approach shapes teachers’ practices and students’ learning and literacy processes. The study was situated in a Year Six classroom in an Australian technology-rich independent school. Data was collected during the 2013 school year and included: 1) observations; 2) 125 hours of classroom video-recordings; 3) a collection of digital artefacts designed by the students; 4) interviews with teachers and students; 5) a student survey regarding technology integration in the classroom; 6) entry logs posted by participants on the Edmodo social network site. Multiple approaches to data analysis were used in order to answer the study’s research questions, including: networked learning analysis, thematic analysis, situated discourse analysis, multimodal discourse analysis and a quantitative descriptive analysis. The findings suggest that blended learning spaces support teachers’ distributed orchestration of classroom activities across tools and resources while also leveraging students’ engagement in reciprocal teaching as well as self-driven and collaborative learning. Digital technologies open space for new ways of communication, interaction and learning in the classroom, yet such affordances are dependent upon teacher’s facilitation and expertise. In addition, an interactive pattern of literacy practices was evident in the classroom, where processes of authorship, readership, production, audience, and consumption were established between students. Finally, alignment between teachers’ beliefs and the perceived value of technology was a key factor for technology integration in the classroom

Learning and out-of-school literacy practices in the digital age: a rural approach

En el marco de la era digital, el presente estudio explora las prácticas de literacidad de niños fuera de la sala de clases y su conexión con procesos de aprendizaje formal en un contexto rural. Utilizando una perspectiva ecológica y sociocultural, el artículo busca conectar áreas de investigación que frecuentemente se encuentran separadas en la literatura, como lo son los estudios centrados en la escuela y aquellos de aprendizaje informal; así como indagar en un área como es la educación rural que se encuentra subestudiada en la literatura. El estudio se sitúa en una localidad rural en Chile. Se utilizó una metodología de estudio de caso múltiple. Los resultados indican una orientación hacia las prácticas de literacidad digital en el hogar, pese a la falta de disponibilidad de tecnologías. Esto contrasta con la existencia de material impreso en los hogares y da cuenta de la importancia de distinguir conceptualmente el acceso y la disponibilidad hacia estos recursos (KALMAN, 2005). Se observa además que las prácticas que llevan a cabo los niños en su día a día son impulsadas por la entretención, la interacción con otros, el juego o el interés en aprender un tema específico. Por último, el análisis indica una discontinuidad con las prácticas de literacidad observadas en la sala de clases.The present study explores children’s out-of-school literacy practices and their connection with processes of formal learning in a rural context. The article aims at bridging research areas that are often disconnected in the literature, such as research on school education and informal learning; along with research on rural education that has been often underresearched in the literature. This research focuses the study of literacy and learning from an ecological and a socio-cultural perspective. The study is situated in a rural area in Chile and uses a multiple-case study design. The results show a trend towards digital literacy practices at home, despite the lack of availability of technologies. This contrasts with the presence of printed materials at home and bespeaks the need to distinguish between access to and availability of these resources (KALMAN, 2005). In addition, children’s everyday practices are motivated by their interest on entertainment, interaction with others, play and the passion to learn a specific topic. Finally, the analysis shows a discontinuity between these practices and literacy practices observed in the classroom

Bacterial lipid II analogs : novel in vitro substrates for mammalian oligosaccharyl diphosphodolichol diphosphatase (DLODP) activities

Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho (PP)-dolichol (DLO) onto proteins in the endoplasmic reticulum (ER). Under some pathophysiological conditions, DLO biosynthesis is perturbed, and truncated DLO is hydrolyzed to yield oligosaccharyl phosphates (OSP) via unidentified mechanisms. DLO diphosphatase activity (DLODP) was described in vitro, but its characterization is hampered by a lack of convenient non-radioactive substrates. Our objective was to develop a fluorescence-based assay for DLO hydrolysis. Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII: GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P). GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity. To conclude, we show, for the first time, hydrolysis of lipid II by a eukaryotic enzyme. As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities

Stepwise disarmament and sudden destruction in a two-person game: a research tool

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66968/2/10.1177_002200276400800104.pd

Enriched biodiversity data as a resource and service

Background: Recent years have seen a surge in projects that produce large volumes of structured, machine-readable biodiversity data. To make these data amenable to processing by generic, open source “data enrichment” workflows, they are increasingly being represented in a variety of standards-compliant interchange formats. Here, we report on an initiative in which software developers and taxonomists came together to address the challenges and highlight the opportunities in the enrichment of such biodiversity data by engaging in intensive, collaborative software development: The Biodiversity Data Enrichment Hackathon. Results: The hackathon brought together 37 participants (including developers and taxonomists, i.e. scientific professionals that gather, identify, name and classify species) from 10 countries: Belgium, Bulgaria, Canada, Finland, Germany, Italy, the Netherlands, New Zealand, the UK, and the US. The participants brought expertise in processing structured data, text mining, development of ontologies, digital identification keys, geographic information systems, niche modeling, natural language processing, provenance annotation, semantic integration, taxonomic name resolution, web service interfaces, workflow tools and visualisation. Most use cases and exemplar data were provided by taxonomists. One goal of the meeting was to facilitate re-use and enhancement of biodiversity knowledge by a broad range of stakeholders, such as taxonomists, systematists, ecologists, niche modelers, informaticians and ontologists. The suggested use cases resulted in nine breakout groups addressing three main themes: i) mobilising heritage biodiversity knowledge; ii) formalising and linking concepts; and iii) addressing interoperability between service platforms. Another goal was to further foster a community of experts in biodiversity informatics and to build human links between research projects and institutions, in response to recent calls to further such integration in this research domain. Conclusions: Beyond deriving prototype solutions for each use case, areas of inadequacy were discussed and are being pursued further. It was striking how many possible applications for biodiversity data there were and how quickly solutions could be put together when the normal constraints to collaboration were broken down for a week. Conversely, mobilising biodiversity knowledge from their silos in heritage literature and natural history collections will continue to require formalisation of the concepts (and the links between them) that define the research domain, as well as increased interoperability between the software platforms that operate on these concepts

Current gene panels account for nearly all homologous recombination repair-associated multiple-case breast cancer families

It was hypothesized that variants in underexplored homologous recombination repair (HR) genes could explain unsolved multiple-case breast cancer (BC) families. We investigated HR deficiency (HRD)-associated mutational signatures and second hits in tumor DNA from familial BC cases. No candidates genes were associated with HRD in 38 probands previously tested negative with gene panels. We conclude it is unlikely that unknown HRD-associated genes explain a large fraction of unsolved familial BC

Macrophage-B Cell Interactions in the Inverted Porcine Lymph Node and Their Response to Porcine Reproductive and Respiratory Syndrome Virus

Swine lymph nodes (LN) present an inverted structure compared to mouse and human, with the afferent lymph diffusing from the center to the periphery. This structure, also observed in close and distant species such as dolphins, hippopotamus, rhinoceros, and elephants, is poorly described, nor are the LN macrophage populations and their relationship with B cell follicles. B cell maturation occurs mainly in LN B cell follicles with the help of LN macrophage populations endowed with different antigen delivery capacities. We identified three macrophage populations that we localized in the inverted LN spatial organization. This allowed us to ascribe porcine LN MΦ to their murine counterparts: subcapsular sinus MΦ, medullary cord MΦ and medullary sinus MΦ. We identified the different intra and extrafollicular stages of LN B cells maturation and explored the interaction of MΦ, drained antigen and follicular B cells. The porcine reproductive and respiratory syndrome virus (PRRSV) is a major porcine pathogen that infects tissue macrophages (MΦ). PRRSV is persistent in the secondary lymphoid tissues and induces a delay in neutralizing antibodies appearance. We observed PRRSV interaction with two LN MΦ populations, of which one interacts closely with centroblasts. We observed BCL6 up-regulation in centroblast upon PRRSV infection, leading to new hypothesis on PRRSV inhibition of B cell maturation. This seminal study of porcine LN will permit fruitful comparison with murine and human LN for a better understanding of normal and inverted LN development and functioning

MVA-CoV2-S Vaccine Candidate Neutralizes Distinct Variants of Concern and Protects Against SARS-CoV-2 Infection in Hamsters

To control the coronavirus disease 2019 (COVID-19) pandemic and the emergence of different variants of concern (VoCs), novel vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed. In this study, we report the potent immunogenicity and efficacy induced in hamsters by a vaccine candidate based on a modified vaccinia virus Ankara (MVA) vector expressing a human codon optimized full-length SARS-CoV-2 spike (S) protein (MVA-S). Immunization with one or two doses of MVA-S elicited high titers of S- and receptor-binding domain (RBD)-binding IgG antibodies and neutralizing antibodies against parental SARS-CoV-2 and VoC alpha, beta, gamma, delta, and omicron. After SARS-CoV-2 challenge, MVA-S-vaccinated hamsters showed a significantly strong reduction of viral RNA and infectious virus in the lungs compared to the MVA-WT control group. Moreover, a marked reduction in lung histopathology was also observed in MVA-S-vaccinated hamsters. These results favor the use of MVA-S as a potential vaccine candidate for SARS-CoV-2 in clinical trials.The authors declare that this study received funding from Fondo COVID-19 grant COV20/00151 [Spanish Health Ministry, Instituto de Salud Carlos III (ISCIII)], Fondo Supera COVID-19 grant (Crue Universidades-Banco Santander) and Spanish Research Council (CSIC) grant 202120E079 (to JG-A), CSIC grant 2020E84, La CaixaImpulse grant CF01-00008, Ferrovial and MAPFRE donations (to ME), a Spanish Ministry of Science and Innovation (MCIN)/Spanish Research Agency (AEI)/10.13039/501100011033 grant (PID2020-114481RB-I00 to JGA and ME), and internal funding from KU Leuven. This research work was also funded by the European Commission-NextGeneration EU through CSIC’s Global Health Platform (PTI Salud Global) (to JG-A and ME). The funders were not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication.Peer reviewe

Effects of Climate Variability and Change on the Population Dynamics of Cephalopods and a Predictive and Forecast Framework to Inform Fisheries Management

AFS Global Conference, 22-23 October 2020, onlineN