Comparison of mechanical ventilatory constraints between continuous and intermittent exercises in healthy prepubescent children.

International audienceBBackground: The aim of this study was to evaluate the occurrence and severity of mechanical ventilatory constraints in healthy prepubescent children during continuous and intermittent exercise. Methods: Twelve prepubescent children (7 - 11 years old) performed 7 exercises on a treadmill: one graded test for the determination of maximal aerobic speed (MAS), three continuous exercises (CE) at 60, 70 and 80% of MAS and three intermittent exercises (IE), alternating 15s of exercise with 15s of passive recovery, at 90, 100 and 110% of MAS. During each CE and IE, tidal flow/volume loops were plotted within a maximal flow/volume loop (MFVL) measured at rest before each exercise. Expiratory flow limitation (expFL expressed in %Vt) was defined as the part of exercise tidal volume (Vt) meeting the boundary of MFVL. Breathing strategy was estimated by measuring inspiratory capacity relative to forced vital capacity and tidal volume relative to inspiratory capacity. Other breathing pattern parameters (ventilation VE, Vt, respiratory frequency f) were continuously recorded during exercise. Results: An "intensity" effect was found for during CE (p<0.001) but not during IE (p=0.08). The increase in was predominantly assumed by an increase in f for both exercise modalities. During each exercise, several children heterogeneously experienced expFL ranging between 10 - 90%Vt. For all exercises, Vt was predominantly regulated by an increase in Vt/IC with no change in IC/FVC from rest to exercise. Finally, no significant "modality" effect was found for mechanical ventilatory constraint parameters (expFL, VT/IC and IV/FVC). Discussion: We could conclude that neither of the modalities studied induced more mechanical ventilatory constraints than the other, but that exercise intensities specific to each modality might be greater sources of exacerbation for mechanical ventilatory constraints

The GenTree Platform: growth traits and tree-level environmental data in 12 European forest tree species

Background: Progress in the field of evolutionary forest ecology has been hampered by the huge challenge of phenotyping trees across their ranges in their natural environments, and the limitation in high-resolution environmental information. Findings: The GenTree Platform contains phenotypic and environmental data from 4,959 trees from 12 ecologically and economically important European forest tree species: Abies alba Mill. (silver fir), Betula pendula Roth. (silver birch), Fagus sylvatica L. (European beech), Picea abies (L.) H. Karst (Norway spruce), Pinus cembra L. (Swiss stone pine), Pinus halepensis Mill. (Aleppo pine), Pinus nigra Arnold (European black pine), Pinus pinaster Aiton (maritime pine), Pinus sylvestris L. (Scots pine), Populus nigra L. (European black poplar), Taxus baccata L. (English yew), and Quercus petraea (Matt.) Liebl. (sessile oak). Phenotypic (height, diameter at breast height, crown size, bark thickness, biomass, straightness, forking, branch angle, fructification), regeneration, environmental in situ measurements (soil depth, vegetation cover, competition indices), and environmental modeling data extracted by using bilinear interpolation accounting for surrounding conditions of each tree (precipitation, temperature, insolation, drought indices) were obtained from trees in 194 sites covering the species’ geographic ranges and reflecting local environmental gradients. Conclusion: The GenTree Platform is a new resource for investigating ecological and evolutionary processes in forest trees. The coherent phenotyping and environmental characterization across 12 species in their European ranges allow for a wide range of analyses from forest ecologists, conservationists, and macro-ecologists. Also, the data here presented can be linked to the GenTree Dendroecological collection, the GenTree Leaf Trait collection, and the GenTree Genomic collection presented elsewhere, which together build the largest evolutionary forest ecology data collection available

Between but not within species variation in the distribution of fitness effects

New mutations provide the raw material for evolution and adaptation. The distribution of fitness effects (DFE) describes the spectrum of effects of new mutations that can occur along a genome, and is therefore of vital interest in evolutionary biology. Recent work has uncovered striking similarities in the DFE between closely related species, prompting us to ask whether there is variation in the DFE among populations of the same species, or among species with different degrees of divergence, i.e., whether there is variation in the DFE at different levels of evolution. Using exome capture data from six tree species sampled across Europe we characterised the DFE for multiple species, and for each species, multiple populations, and investigated the factors potentially influencing the DFE, such as demography, population divergence and genetic background. We find statistical support for there being variation in the DFE at the species level, even among relatively closely related species. However, we find very little difference at the population level, suggesting that differences in the DFE are primarily driven by deep features of species biology, and that evolutionarily recent events, such as demographic changes and local adaptation, have little impact

Facteurs de variation de la cinétique de VO2 lors de deux modalités d'exercice (Intermittente / Continue) chez des spécialistes de demi-fond et de fond

NANTES-BU Sciences (441092104) / SudocSudocFranceF

Réponses physiologiques à l'exercice intermittent maximal sur piste et sur tapis roulant

Un exercice intermittent maximal réalisé sur piste en condition extérieure semble induire une sollicitation métabolique (exprimée par les valeurs de tVO2max, calcul du temps passé à VO2 max, et par la lactatémie de fin d'exercice) plus importante que lorsqu'il est réalisé en laboratoire sur tapis roulant avec une pente de 1%

Réponses physiologiques à l'exercice intermittent maximal sur piste et sur tapis roulant

Un exercice intermittent maximal réalisé sur piste en condition extérieure semble induire une sollicitation métabolique (exprimée par les valeurs de tVO2max, calcul du temps passé à VO2 max, et par la lactatémie de fin d'exercice) plus importante que lorsqu'il est réalisé en laboratoire sur tapis roulant avec une pente de 1%

Influence of recovery mode (passive vs. active) on time spent at maximal oxygen uptake during an intermittent session in young and endurance-trained athletes.

International audienceThe aim of this study was to analyze the effects of recovery mode (active/passive) on time spent at high percentage of maximal oxygen uptake (VO2max) i.e. above 90% of VO2max (t90VO2max) and above 95% of VO2max (t95VO2max) during a single short intermittent session. Eight endurance-trained male adolescents (15.9 +/- 1.4 years) performed three field tests until exhaustion: a graded test to determine their VO2max (57.4 +/- 6.1 ml min(-1) kg(-1)), and maximal aerobic velocity (MAV; 17.9 +/- 0.4 km h(-1)), and in a random order, two intermittent exercises consisting of repeated 30 s runs at 105% of MAV alternated with 30 s passive (IE(P)) or active recovery (IE(A), 50% of MAV). Time to exhaustion (t(lim)) was significantly longer for IE(P) than for IE(A) (2145 +/- 829 vs. 1072 +/- 388 s, P < 0.01). No difference was found in t90VO2max and t95VO2max between IE(P) (548 +/- 499-316 +/- 360 s) and IE(A) (746 +/- 417-459 +/- 332 s). However, when expressed as a percentage of t(lim), t90VO2max and t95VO2max were significantly longer (P < 0.001 and P < 0.05, respectively) during IE(A) (67.7 +/- 19%-42.1 +/- 27%) than during IE(P) (24.2 +/- 19%-13.8 +/- 15%). Our results demonstrated no influence of recovery mode on absolute t90VO2max or t95VO2max mean values despite significantly longer t(lim) values for IE(P) than for IE(A). In conclusion, passive recovery allows a longer running time (t(lim)) for a similar time spent at a high percentage of VO2max

Assessment of child-specific aerobic fitness and anaerobic capacity by the use of the power-time relationships constants

International audienc

Mechanical ventilatory constraints during incremental exercise in healthy and cystic fibrosis children.

International audienceTo analyze breathing pattern and mechanical ventilatory constraints during incremental exercise in healthy and cystic fibrosis (CF) children. Thirteen healthy children and 6 children with cystic fibrosis volunteered to perform an incremental test on a treadmill. Exercise tidal flow/volume loops were plotted every minute within a maximal flow/volume loop (MFVL). Expiratory flow limitation (expFL expressed in %Vt) was evaluated and end-expiratory and end-inspiratory lung volumes (EELV and EILV) were estimated from expiratory reserve volume relative to vital capacity (ERV/FVC) and from inspiratory reserve volume relative to vital capacity (IRV/FVC). During the incremental exercise, expFL was first observed at 40% of maximal aerobic speed in both groups. At maximal exercise, 46% of healthy children and 83% of CF children presented expFL, without significant effect of cystic fibrosis on the severity of expFL. According to the two-way ANOVA results, both groups adopted similar breathing pattern and breathing strategies as no significant effect of CF has been revealed. But, according to one-way ANOVA results, a significant increase of ERV/FVC associated with a significant decrease of IRV/FVC from resting value shave been observed in healthy children at maximal exercise, but not in CF children. The hypothesis of this study was based on the assumption that mild cystic fibrosis could induce more frequent and more severe mechanical ventilatory constraints due to pulmonary impairment and breathing pattern disturbances. But, this study did not succeed to highlight an effect of mild cystic fibrosis on the mechanical ventilatory constraints (expFL and dynamic hyperinflation) that occur during an incremental exercise. This absence of effect could be due to the absence of an impact of the disease on spirometric data, breathing pattern regulation during exercise and breathing strategy