Development of a mixed-meal that impacts carotid-body mediated cardiorespiratory and metabolic parameters - a pilot study

The carotid bodies (CB) have been recently implicated in the genesis of metabolic diseases in animal models. The CBs respond to circulating insulin to increase heart rate, respiratory rate and blood glucose levels. In humans, this mechanism is not well characterized.info:eu-repo/semantics/publishedVersio

Métodos não farmacológicos no alívio da dor em neonatos prematuros

The aim was to know the non-pharmacological methods of pain relief in premature newborns admitted to the Neonatal Intensive Care Unit produced in the scientific literature on the subject. This is an integrative review. The following descriptors were used: "premature newborn", "pain management", "premature baby" and "pain". The following databases were used: Medical Literature Analysis and Retrieval System Online, Latin American and Caribbean Literature in Health Sciences, Nursing Database, Bibliographic Index Español en Ciencias de la Salud and Bibliografía Nacional en Ciencias de la Salud Argentina being found 275 articles. In the studies found that composed the sample (36 articles), it was possible to observe the following non-pharmacological techniques for pain relief: sucrose, breast milk, facilitated containment, skin-to-skin care, oral glucose 25%, non-nutritive sucking, kangaroo method, lullabies, winding up, kangaroo mother care, white noise, breastfeeding, pacifier, etc. It is noteworthy that 16.17% of the studies refer to the analgesia potential with sucrose, both alone and in combination with some other method. It is observed that some pain relief strategies that can be used and applied by the team responsible for care in the neonatal intensive care unit, expanding the view that simple alternatives can be effective to bring comfort to these babies.Objetivou-se conhecer os métodos não farmacológicos no alívio da dor nos recém-nascidos prematuros internados na Unidade de Terapia Intensiva Neonatal produzidos na literatura científica sobre o tema. Trata-se de uma revisão integrativa. Utilizou-se os seguintes descritores: “recém-nascido prematuro", “manejo da dor" “bebê prematuro” e “dor”. Utilizou-se as bases de dados: Medical Literature Analysis and Retrieval System Online, Literatura Latino-Americana e do Caribe em Ciências da Saúde, Base de Dados de Enfermagem, Índice Bibliográfico Español en Ciencias de la Salud e Bibliografía Nacional en Ciencias de la Salud Argentina sendo encontrados 275 artigos. Nos estudos encontrados que compuseram a amostra (36 artigos), foi  possível observar as seguintes técnicas não farmacológicas para alívio de dor: sacarose, leite materno, contenção facilitada, cuidado pele a pele, glicose oral 25%, sucção não nutritiva, método canguru, canções de ninar, enrolamento, método mãe-canguru, ruído branco, amamentação, chupeta etc.  Destaca-se que 16,17% dos estudos referem-se ao potencial de analgesia com sacarose, tanto isoladamente quanto em combinação com algum outro método. Observa-se que algumas estratégias de alívio da dor que podem ser utilizadas e aplicadas pela equipe responsável pelos cuidados na unidade de terapia intensiva neonatal, ampliando a visão de que alternativas simples podem ser eficazes para trazer conforto a esses bebês

Métodos não farmacológicos no alívio da dor em neonatos prematuros

The aim was to know the non-pharmacological methods of pain relief in premature newborns admitted to the Neonatal Intensive Care Unit produced in the scientific literature on the subject. This is an integrative review. The following descriptors were used: "premature newborn", "pain management", "premature baby" and "pain". The following databases were used: Medical Literature Analysis and Retrieval System Online, Latin American and Caribbean Literature in Health Sciences, Nursing Database, Bibliographic Index Español en Ciencias de la Salud and Bibliografía Nacional en Ciencias de la Salud Argentina being found 275 articles. In the studies found that composed the sample (36 articles), it was possible to observe the following non-pharmacological techniques for pain relief: sucrose, breast milk, facilitated containment, skin-to-skin care, oral glucose 25%, non-nutritive sucking, kangaroo method, lullabies, winding up, kangaroo mother care, white noise, breastfeeding, pacifier, etc. It is noteworthy that 16.17% of the studies refer to the analgesia potential with sucrose, both alone and in combination with some other method. It is observed that some pain relief strategies that can be used and applied by the team responsible for care in the neonatal intensive care unit, expanding the view that simple alternatives can be effective to bring comfort to these babies.Objetivou-se conhecer os métodos não farmacológicos no alívio da dor nos recém-nascidos prematuros internados na Unidade de Terapia Intensiva Neonatal produzidos na literatura científica sobre o tema. Trata-se de uma revisão integrativa. Utilizou-se os seguintes descritores: “recém-nascido prematuro", “manejo da dor" “bebê prematuro” e “dor”. Utilizou-se as bases de dados: Medical Literature Analysis and Retrieval System Online, Literatura Latino-Americana e do Caribe em Ciências da Saúde, Base de Dados de Enfermagem, Índice Bibliográfico Español en Ciencias de la Salud e Bibliografía Nacional en Ciencias de la Salud Argentina sendo encontrados 275 artigos. Nos estudos encontrados que compuseram a amostra (36 artigos), foi  possível observar as seguintes técnicas não farmacológicas para alívio de dor: sacarose, leite materno, contenção facilitada, cuidado pele a pele, glicose oral 25%, sucção não nutritiva, método canguru, canções de ninar, enrolamento, método mãe-canguru, ruído branco, amamentação, chupeta etc.  Destaca-se que 16,17% dos estudos referem-se ao potencial de analgesia com sacarose, tanto isoladamente quanto em combinação com algum outro método. Observa-se que algumas estratégias de alívio da dor que podem ser utilizadas e aplicadas pela equipe responsável pelos cuidados na unidade de terapia intensiva neonatal, ampliando a visão de que alternativas simples podem ser eficazes para trazer conforto a esses bebês

Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma

The lung is a complex ecosystem of host cells and microbes often disrupted in pathological conditions. Although bacteria have been hypothesized as agents of carcinogenesis, little is known about microbiota profile of the most prevalent cancer subtypes: adenocarcinoma (ADC) and squamous cell carcinoma (SCC). To characterize lung cancer (LC) microbiota a first a screening was performed through a pooled sequencing approach of 16S ribosomal RNA gene (V3-V6) using a total of 103 bronchoalveaolar lavage fluid samples. Then, identified taxa were used to inspect 1009 cases from The Cancer Genome Atlas and to annotate tumor unmapped RNAseq reads. Microbial diversity was analyzed per cancer subtype, history of cigarette smoking and airflow obstruction, among other clinical data. We show that LC microbiota is enriched in Proteobacteria and more diverse in SCC than ADC, particularly in males and heavier smokers. High frequencies of Proteobacteria were found to discriminate a major cluster, further subdivided into well-defined communities’ associated with either ADC or SCC. Here, a SCC subcluster differing from other cases by a worse survival was correlated with several Enterobacteriaceae. Overall, this study provides first evidence for a correlation between lung microbiota and cancer subtype and for its influence on patient life expectancy.We would like to thank all patients for donating their samples and for collaborating in this study. IPATIMUP integrates the i3S Research Unit, which is partially supported by the Portuguese Foundation for Science and Technology (FCT). This work was supported by the Portuguese Foundation for Science and Technology (FCT), financed by the European Social Funds (COMPETE-FEDER) and National Funds through the FCT (projects PEstC/SAU/LA0003/2013 and POCI-01-0145-FEDER-007274, fellowships SFRH/BPD/77646/2011 and SFRH/BPD/120777/2016 to S.G. and P.I.M., respectively, grant PTDC/BEXGMG/0242/2012 to S.S. and by Programa Operacional Regional do Norte (ON.2 – O Novo Norte and Norte 2020), through FEDER funds under the Quadro de Referência Estratégico Nacional (QREN; projects NORTE-07-0162-FEDER-00018 and NORTE-070162-FEDER-000067, and NORTE-01-0145-FEDER-000029)

acute complications and long-term results

Background: An ablation catheter and a circular mapping catheter requiring a double transeptal puncture (TSP) for left atrial access have been conventionally used for atrial fibrillation (AF) ablation. Recently, different operators have combined a single transseptal puncture technique with 3D high-density mapping catheters for pulmonary vein isolation (PVI). Objective: This study aims to compare two strategies, single vs. double TSP, regarding the duration of the procedure, radiation time, complication rates, and outcomes. Methods: Retrospective analysis of a large cohort of consecutive patients that underwent first PVI with radiofrequency energy (RF), using a point-by-point strategy, with a 3D mapping system, either with single or double TSP, according to the operator's choice. Results: 285 patients with a mean age of 59.5 ± 11.6 years (36.5% female, 67.7% paroxysmal AF) underwent a point-by-point catheter ablation with RF between July 2015 and March 2020. The mean CHA2DS2-VASc score was 1.7 ± 1.3. Single TSP was performed in 115 (40.3%) patients and double TSP in 170 (59.6%). The operator's experience (≥5 years of AF ablation procedures) was equally distributed among the two groups. The average procedure time (133 ± 31.7 min vs. 123 ± 35.5 min, for single and double TSP, respectively) did reach a statistical difference between both groups (p = 0.008), but there was a substantial advantage regarding fluoroscopy time (13 ± 6.3 min vs. 19 ± 9.1 min, for single and double TSP, respectively; p < 0.001). Acute major complications present similar rates in both groups (2.6% vs. 2.3%, p = 0.799). At the 2-year follow-up, both groups had a similar sinus rhythm maintenance rate (76.5% vs. 78.8%, p = 0.646). Conclusion: A simplified single-TSP technique using high-density multi-electrode 3D mapping is a safe and highly successful option for AF ablation. This approach yields a substantial reduction in fluoroscopy time, with the potential to avoid acute complications, compared to a conventional double-TSP strategy.publishersversionpublishe

Preclinical assessment of mesenchymal-stem-cell-based therapies in spinocerebellar ataxia type 3

The low regeneration potential of the central nervous system (CNS) represents a challenge for the development of new therapeutic strategies for neurodegenerative diseases, including spinocerebellar ataxias. Spinocerebellar ataxia type 3 (SCA3)—or Machado–Joseph disease (MJD)—is the most common dominant ataxia, being mainly characterized by motor deficits; however, SCA3/MJD has a complex and heterogeneous pathophysiology, involving many CNS brain regions, contributing to the lack of effective therapies. Mesenchymal stem cells (MSCs) have been proposed as a potential therapeutic tool for CNS disorders. Beyond their differentiation potential, MSCs secrete a broad range of neuroregulatory factors that can promote relevant neuroprotective and immunomodulatory actions in different pathophysiological contexts. The objective of this work was to study the effects of (1) human MSC transplantation and (2) human MSC secretome (CM) administration on disease progression in vivo, using the CMVMJD135 mouse model of SCA3/MJD. Our results showed that a single CM administration was more beneficial than MSC transplantation—particularly in the cerebellum and basal ganglia—while no motor improvement was observed when these cell-based therapeutic approaches were applied in the spinal cord. However, the effects observed were mild and transient, suggesting that continuous or repeated administration would be needed, which should be further tested.This research was funded by the National Ataxia Foundation (NAF) and by Portuguese national funds, through the Foundation for Science and Technology (FCT)—projects UIDB/50026/2020, UIDP/50026/2020, POCI-01-0145-FEDER-029206, and through the Santa Casa Neuroscience Awards (Santa Casa da Misericórdia Lisboa)—project MC-04/17. Additionally, this project was funded by the ICVS Scientific Microscopy Platform, a member of the national infrastructure PPBI—Portuguese Platform of Bioimaging (PPBI-POCI-01-0145-FEDER-022122). S.C.S. received an individual fellowship within the project TUBITAK/0007/2014. The FCT funded individual fellowships to J.S C., A.N.-C., B.M.- P., F.G.T., R.L., S.M., N.A.S., C.S.-C., and S.D.-S. (SFRH/BD/140624/2018, SFRH/BPD/118779/2016, SFRH/BD/120124/2016, SFRH/BPD/118408/2016, PD/BDE/127836/2016, CEECIND/01902/2017, CEECIND/04794/2017, CEECIND/03887/2017, and CEECIND/00685/2020)

Patient-physician discordance in assessment of adherence to inhaled controller medication: a cross-sectional analysis of two cohorts

We aimed to compare patient's and physician's ratings of inhaled medication adherence and to identify predictors of patient-physician discordance.(SFRH/BPD/115169/2016) funded by Fundação para a Ciência e Tecnologia (FCT); ERDF (European Regional Development Fund) through the operations: POCI-01-0145-FEDER-029130 ('mINSPIRERS—mHealth to measure and improve adherence to medication in chronic obstructive respiratory diseases—generalisation and evaluation of gamification, peer support and advanced image processing technologies') cofunded by the COMPETE2020 (Programa Operacional Competitividade e Internacionalização), Portugal 2020 and by Portuguese Funds through FCT (Fundação para a Ciência e a Tecnologia).info:eu-repo/semantics/publishedVersio

Enxaqueca hemiplégica familiar - um relato de caso / Familial hemiplegic migraine - a case report

A enxaqueca hemiplégica familiar (EHF) é um subtipo de enxaqueca com aura, caracterizado por fraqueza motora, em que ao menos um familiar de primeiro ou segundo grau também possua o mesmo diagnóstico. A sua sintomatologia está relacionada aos sintomas clássicos da aura, descritos acima, e à cefaleia, logo, o paciente pode apresentar perturbações na consciência, hemianopsia, afasia, letargia, hemiparesia, disfagia, febre, confusão mental, sendo, muitas vezes, confundida e tratada como epilepsia (2–6)