Antitumor immune cycle proteins and HPV status in patients with HSIL and cervical cancer

Background. Cervical cancer (CC) is a common malignant tumor among women worldwide. The anti-tumor immune cycle (AIC) is a necessary molecular mechanism that prevents the occurrence and progression of a tumor. It is known that during the development of CC, several mechanisms disrupt the AIC and contribute to tumor progression. Recent data show the role of human papillomavirus (HPV) in the AIC regulation as a mechanism for the emergence of tumor resistance to the anti-tumor immune response. Aim. To study the levels of AIC proteins (sCD25, 4-1BB, B7.2, TGF-b1, CTLA-4, PD-L1, PD-1, Tim-3, LAG-3, Galectin-9, sCD27, PD-L2) in patients with high-grade squamous intraepithelial lesion (HSIL) and CC, depending on the HPV status. Materials and methods. A prospective study enrolled women of reproductive age with HSIL (n=53) and stage IIII СС (n=93). The control group included female volunteers without cervical abnormalities (n=30). The study material was the cervical epithelium. Study methods: flow cytometry, diagnostics using the polymerase chain reaction for HPV status and viral load. Statistical processing was performed using the IBM SPSS Statistics version 25.0 software package using non-parametric statistics methods. Results. The obtained data indicate an expression increase of AIC inhibitors: PD-1 and PD-L2 in patients with HPV infection and sCD27 in patients with mono-HPV infection. There were no significant differences in the levels of AIC proteins, depending on the HPV viral load in patients with HSIL and CC. Conclusion. The effect of HPV infection and its type on regulating the expression of specific AIC proteins has been established, which is one of the mechanisms of tumor progression

Ophiolite association of Cape Fiolent (western part of the Mountainous Crimea) – the upper age constraint according to the U-Pb isotope dating of plagiorhyolites (Monakh Cliff)

The article presents the results of U-Pb isotope dating (SHRIMP-II, VSEGEI, Saint Petersburg) of zircon crystals extracted from plagiorhyolites of the Monakh Cliff in the area of Cape Fiolent in the western part of the Mountainous Crimea (southern suburb of Sevastopol). a concordant age estimate of 168.3±1.3 Ma was obtained from 20 zircon crystals. It exactly corresponds to the Bajocian/Bathonian boundary of the Middle Jurassic according to the International Chronostratigraphic Chart (February 2022 version). The available results of isotope dating of igneous rocks from the Mountainous Crimea, as well as their geochemical typification are synthesised. The plagiorhyolites of the Monakh Cliff in the area of Cape Fiolent are spatially, and most likely paragenetically, associated with the wallrock (Cape Vinogradny) and ore (Heraclea Plateau on the cognominal peninsula) massive sulphide formations, as well as pillow basalts, gabbroids, and serpentinized hyperbasites, combined into the ophiolite association of Cape Fiolent. The obtained dating is the upper age limit for the entire ophiolite association of Cape Fiolent

ЧЕЛОВЕЧЕСКИЙ КАПИТАЛ РЕГИОНА: ПРОБЛЕМЫ СУЩНОСТИ, СТРУКТУРЫ И ОЦЕНКИ

This article considers main questions appear in comtemporary circumstances while studying human capital on regional level: problems of understanding and aggregation, human capital structuring and assessment.В данной статье рассматриваются основные вопросы, которые возникают в современных условиях при исследовании человеческого капитала на региональном уровне: проблемы понимания и агрегирования, структурирования и оценки человеческого капитала

REGIONAL HUMAN CAPITAL: PROBLEMS OF ESSENCE, STRUCTURE AND ASSESSMENT

This article considers main questions appear in comtemporary circumstances while studying human capital on regional level: problems of understanding and aggregation, human capital structuring and assessment

Yeast Short-Lived Actin-Associated Protein Forms a Metastable Prion in Response to Thermal Stress

Self-perpetuating ordered protein aggregates (amyloids and prions) are associated with a variety of neurodegenerative disorders. Although environmental agents have been linked to certain amyloid diseases, the molecular basis of their action remains unclear. We have employed endogenous yeast prions as a model system to study environmental control of amyloid formation. A short-lived actin-associated yeast protein Lsb2 can trigger prion formation by other proteins in a mode regulated by the cytoskeleton and ubiquitin-dependent processes. Here, we show that such a heterologous prion induction is due to the ability of Lsb2 to form a transient prion state, generated in response to thermal stress. Evolutionary acquisition of prion-inducing activity by Lsb2 is traced to a single amino acid change, coinciding with the acquisition of thermotolerance in the Saccharomyces yeast lineage. This raises the intriguing possibility that the transient prion formation could aid in functioning of Lsb2 at higher temperatures