Multiple episodes of star formation in the CN15/16/17 molecular complex

We have started a campaign to identify massive star clusters inside bright molecular bubbles towards the Galactic Center. The CN15/16/17 molecular complex is the first example of our study. The region is characterized by the presence of two young clusters, DB10 and DB11, visible in the NIR, an ultra-compact HII region identified in the radio, several young stellar objects visible in the MIR, a bright diffuse nebulosity at 8\mu m coming from PAHs and sub-mm continuum emission revealing the presence of cold dust. Given its position on the sky (l=0.58, b=-0.85) and its kinematic distance of ~7.5 kpc, the region was thought to be a very massive site of star formation in proximity of the CMZ. The cluster DB11 was estimated to be as massive as 10^4 M_sun. However the region's properties were known only through photometry and its kinematic distance was very uncertain given its location at the tangential point. We aimed at better characterizing the region and assess whether it could be a site of massive star formation located close to the Galactic Center. We have obtained NTT/SofI JHKs photometry and long slit K band spectroscopy of the brightest members. We have additionally collected data in the radio, sub-mm and mid infrared, resulting in a quite different picture of the region. We have confirmed the presence of massive early B type stars and have derived a spectro-photometric distance of ~1.2 kpc, much smaller than the kinematic distance. Adopting this distance we obtain clusters masses of M(DB10) ~ 170 M_sun and M(DB11) ~ 275 M_sun. This is consistent with the absence of any O star, confirmed by the excitation/ionization status of the nebula. No HeI diffuse emission is detected in our spectroscopic observations at 2.113\mu m, which would be expected if the region was hosting more massive stars. Radio continuum measurements are also consistent with the region hosting at most early B stars.Comment: Accepted for publication in Astronomy and Astrophysics. Fig. 1 and 3 presented in reduced resolutio

Search for starless clumps in the ATLASGAL survey

In this study, we present an unbiased sample of the earliest stages of massive star formation across 20 square-degree of the sky. Within the region 10deg < l < 20deg and |b| < 1deg, we search the ATLASGAL survey at 870 micron for dense gas condensations. These clumps are carefully examined for indications of ongoing star formation using YSOs from the GLIMPSE source catalog as well as sources in the 24 micron MIPSGAL images, to search for starless clumps. We calculate the column densities as well as the kinematic distances and masses for sources where the v_lsr is known from spectroscopic observations. Within the given region, we identify 210 starless clumps with peak column densities > 1 x 10e23 cm^(-2). In particular, we identify potential starless clumps on the other side of the Galaxy. The sizes of the clumps range between 0.1 pc and 3 pc with masses between a few tens of solar masses up to several ten thousands of solar masses. Most of them may form massive stars, but in the 20 square-degree we only find 14 regions massive enough to form stars more massive than 20 solar masses and 3 regions with the potential to form stars more massive than 40 40 solar masses. The slope of the high-mass tail of the clump mass function for clumps on the near side of the Galaxy is 2.2 and, therefore, Salpeter-like. We estimate the lifetime of the most massive starless clumps to be 60000 yr. The sample offers a uniform selection of starless clumps. In the large area surveyed, we only find a few potential precursors of stars in the excess of 40 solar masses. It appears that the lifetime of these clumps is somewhat shorter than their free-fall times, although both values agree within the errors. In addition, these are ideal objects for detailed studies and follow-up observations.Comment: 15 pages plus appendix, in total 44 pages, accepted for publication in Astronomy & Astrophysics, full tables will be added soo

Фізико-хімічна геотехнологія

Розглянуто принципові засади геотехнологічного видобування різнома- нітних корисних копалин. Викладено питання розкриття та підготовки родовищ за допомогою свердловинної розробки, проаналізовано способи буріння і кріп- лення геотехнологічних свердловин, а такж застосоване обладнання. Розкрито сутність технологічних процесів, які виконуються при диспергуванні гірських порід, розчиненні солей, вилуговуванні металів, підземній виплавці сірки і га- зифікації вугілля, видобуванні в’язкої нафти та сланцьового газу. Навчальний посібник призначений для студентів, які навчаються за спе- ціальністю «Розробка родовищ та видобування корисних копалин», а також для студентів інших спеціальностей гірничих вузів і факультетів та інженерно- технічних працівників підприємств і проектних організацій гірничовидобувних галузей промисловості України

Novel multiple sclerosis susceptibility loci implicated in epigenetic regulation

We conducted a genome-wide association study (GWAS) on multiple sclerosis (MS) susceptibility in German cohorts with 4888 cases and 10,395 controls. In addition to associations within the major histocompatibility complex (MHC) region, 15 non-MHC loci reached genome-wide significance. Four of these loci are novel MS susceptibility loci. They map to the genes L3MBTL3, MAZ, ERG, and SHMT1. The lead variant at SHMT1 was replicated in an independent Sardinian cohort. Products of the genes L3MBTL3, MAZ, and ERG play important roles in immune cell regulation. SHMT1 encodes a serine hydroxymethyltransferase catalyzing the transfer of a carbon unit to the folate cycle. This reaction is required for regulation of methylation homeostasis, which is important for establishment and maintenance of epigenetic signatures. Our GWAS approach in a defined population with limited genetic substructure detected associations not found in larger, more heterogeneous cohorts, thus providing new clues regarding MS pathogenesis

Clinical implications of serum neurofilament in newly diagnosed MS patients: a longitudinal multicentre cohort study

BACKGROUND: We aim to evaluate serum neurofilament light chain (sNfL), indicating neuroaxonal damage, as a biomarker at diagnosis in a large cohort of early multiple sclerosis (MS) patients. METHODS: In a multicentre prospective longitudinal observational cohort, patients with newly diagnosed relapsing-remitting MS (RRMS) or clinically isolated syndrome (CIS) were recruited between August 2010 and November 2015 in 22 centers. Clinical parameters, MRI, and sNfL levels (measured by single molecule array) were assessed at baseline and up to four-year follow-up. FINDINGS: Of 814 patients, 54.7% (445) were diagnosed with RRMS and 45.3% (369) with CIS when applying 2010 McDonald criteria (RRMS[2010] and CIS[2010]). After reclassification of CIS[2010] patients with existing CSF analysis, according to 2017 criteria, sNfL levels were lower in CIS[2017] than RRMS[2017] patients (9.1 pg/ml, IQR 6.2-13.7 pg/ml, n = 45; 10.8 pg/ml, IQR 7.4-20.1 pg/ml, n = 213; p = 0.036). sNfL levels correlated with number of T2 and Gd+ lesions at baseline and future clinical relapses. Patients receiving disease-modifying therapy (DMT) during the first four years had higher baseline sNfL levels than DMT-naïve patients (11.8 pg/ml, IQR 7.5-20.7 pg/ml, n = 726; 9.7 pg/ml, IQR 6.4-15.3 pg/ml, n = 88). Therapy escalation decisions within this period were reflected by longitudinal changes in sNfL levels. INTERPRETATION: Assessment of sNfL increases diagnostic accuracy, is associated with disease course prognosis and may, particularly when measured longitudinally, facilitate therapeutic decisions

A separated vortex ring underlies the flight of the dandelion

Wind-dispersed plants have evolved ingenious ways to lift their seeds1,2. The common dandelion uses a bundle of drag-enhancing bristles (the pappus) that helps to keep their seeds aloft. This passive flight mechanism is highly effective, enabling seed dispersal over formidable distances3,4; however, the physics underpinning pappus-mediated flight remains unresolved. Here we visualized the flow around dandelion seeds, uncovering an extraordinary type of vortex. This vortex is a ring of recirculating fluid, which is detached owing to the flow passing through the pappus. We hypothesized that the circular disk-like geometry and the porosity of the pappus are the key design features that enable the formation of the separated vortex ring. The porosity gradient was surveyed using microfabricated disks, and a disk with a similar porosity was found to be able to recapitulate the flow behaviour of the pappus. The porosity of the dandelion pappus appears to be tuned precisely to stabilize the vortex, while maximizing aerodynamic loading and minimizing material requirements. The discovery of the separated vortex ring provides evidence of the existence of a new class of fluid behaviour around fluid-immersed bodies that may underlie locomotion, weight reduction and particle retention in biological and manmade structures

Intravenous Immunoglobulin Treatment in Multifocal Motor Neuropathy

# The Author(s) 2010. This article is published with open access at Springerlink.com Introduction Multifocal motor neuropathy (MMN) is characterized by asymmetric weakness of limbs and the electrophysiological finding of conduction block in motor nerves. Conduction block is the inability of nerves to propagate action potentials and is probably caused b

IL-17+ CD8+ T cell suppression by dimethyl fumarate associates with clinical response in multiple sclerosis

IL-17-producing CD8+ (Tc17) cells are enriched in active lesions of patients with multiple sclerosis (MS), suggesting a role in the pathogenesis of autoimmunity. Here we show that amelioration of MS by dimethyl fumarate (DMF), a mechanistically elusive drug, associates with suppression of Tc17 cells. DMF treatment results in reduced frequency of Tc17, contrary to Th17 cells, and in a decreased ratio of the regulators RORC-to-TBX21, along with a shift towards cytotoxic T lymphocyte gene expression signature in CD8+ T cells from MS patients. Mechanistically, DMF potentiates the PI3K-AKT-FOXO1-T-BET pathway, thereby limiting IL-17 and RORγt expression as well as STAT5-signaling in a glutathione-dependent manner. This results in chromatin remodeling at the Il17 locus. Consequently, T-BET-deficiency in mice or inhibition of PI3K-AKT, STAT5 or reactive oxygen species prevents DMF-mediated Tc17 suppression. Overall, our data disclose a DMF-AKT-T-BET driven immune modulation and suggest putative therapy targets in MS and beyond

Analysis and Functional Consequences of Increased Fab-Sialylation of Intravenous Immunoglobulin (IVIG) after Lectin Fractionation

It has been proposed that the anti-inflammatory effects of intravenous immunoglobulin (IVIG) might be due to the small fraction of Fc-sialylated IgG. In this study we biochemically and functionally characterized sialic acid-enriched IgG obtained by Sambucus nigra agglutinin (SNA) lectin fractionation. Two main IgG fractions isolated by elution with lactose (E1) or acidified lactose (E2) were analyzed for total IgG, F(ab’)2 and Fc-specific sialic acid content, their pattern of specific antibodies and anti-inflammatory potential in a human in vitro inflammation system based on LPS- or PHA-stimulated whole blood. HPLC and LC-MS testing revealed an increase of sialylated IgG in E1 and more substantially in the E2 fraction. Significantly, the increased amount of sialic acid residues was primarily found in the Fab region whereas only a minor increase was observed in the Fc region. This indicates preferential binding of the Fab sialic acid to SNA. ELISA analyses of a representative range of pathogen and auto-antigens indicated a skewed antibody pattern of the sialylated IVIG fractions. Finally, the E2 fraction exerted a more profound anti-inflammatory effect compared to E1 or IVIG, evidenced by reduced CD54 expression on monocytes and reduced secretion of MCP-1 (CCL2); again these effects were Fab- but not Fc-dependent. Our results show that SNA fractionation of IVIG yields a minor fraction (approx. 10%) of highly sialylated IgG, wherein the sialic acid is mainly found in the Fab region. The tested anti-inflammatory activity was associated with Fab not Fc sialylation