SCREENING TEST FOR THE POTENTIAL RISK OF ACL RUPTURE OF FEMALE AND MALE SOCCER PLAYERS

ACL rupture is a devastating injury. An analysis of risk factors and a consecutive preven-tion program might help to reduce the risk of injury. However there exists no screening test to identify the individual ACL-injury risk. The purpose of this study was to develop a screening test by which the potential risk of ACL rupture for female and male soccer players can be estimated. Testing procedure focussed on dynamical knee valgus in fron-tal plane during landing of a drop jump in normal and fatigued state. The results were ob-tained by 2D video analysis. Results show a wide range of dynamical knee valgus in both sexes with a 5.4 cm greater dynamical valgus of women emphasizing the distinctly higher risk potential for ACL injury of women. Screening tests seem to be suitable to achieve an estimation of the individual risk for ACL injuries without large expenditure

Phylogenetic relationships of cone snails endemic to Cabo Verde based on mitochondrial genomes

Background: Due to their great species and ecological diversity as well as their capacity to produce hundreds of different toxins, cone snails are of interest to evolutionary biologists, pharmacologists and amateur naturalists alike. Taxonomic identification of cone snails still relies mostly on the shape, color, and banding patterns of the shell. However, these phenotypic traits are prone to homoplasy. Therefore, the consistent use of genetic data for species delimitation and phylogenetic inference in this apparently hyperdiverse group is largely wanting. Here, we reconstruct the phylogeny of the cones endemic to Cabo Verde archipelago, a well-known radiation of the group, using mitochondrial (mt) genomes. Results: The reconstructed phylogeny grouped the analyzed species into two main clades, one including Kalloconus from West Africa sister to Trovaoconus from Cabo Verde and the other with a paraphyletic Lautoconus due to the sister group relationship of Africonus from Cabo Verde and Lautoconus ventricosus from Mediterranean Sea and neighboring Atlantic Ocean to the exclusion of Lautoconus endemic to Senegal (plus Lautoconus guanche from Mauritania, Morocco, and Canary Islands). Within Trovaoconus, up to three main lineages could be distinguished. The clade of Africonus included four main lineages (named I to IV), each further subdivided into two monophyletic groups. The reconstructed phylogeny allowed inferring the evolution of the radula in the studied lineages as well as biogeographic patterns. The number of cone species endemic to Cabo Verde was revised under the light of sequence divergence data and the inferred phylogenetic relationships. Conclusions: The sequence divergence between continental members of the genus Kalloconus and island endemics ascribed to the genus Trovaoconus is low, prompting for synonymization of the latter. The genus Lautoconus is paraphyletic. Lautoconus ventricosus is the closest living sister group of genus Africonus. Diversification of Africonus was in allopatry due to the direct development nature of their larvae and mainly triggered by eustatic sea level changes during the Miocene-Pliocene. Our study confirms the diversity of cone endemic to Cabo Verde but significantly reduces the number of valid species. Applying a sequence divergence threshold, the number of valid species within the sampled Africonus is reduced to half.Spanish Ministry of Science and Innovation [CGL2013-45211-C2-2-P, CGL2016-75255-C2-1-P, BES-2011-051469, BES-2014-069575, Doctorado Nacional-567]info:eu-repo/semantics/publishedVersio

In vitro evaluation of a novel bioreactor based on an integral oxygenator and a spirally wound nonwoven polyester matrix for hepatocyte culture as small aggregates

BACKGROUND/AIMS: The development of custom-made bioreactors for use as a bioartificial liver (BAL) is considered to be one of the last challenges on the road to successful temporary extracorporeal liver support therapy. We devised a novel bioreactor (patent pending) which allows individual perfusion of high density cultured hepatocytes with low diffusional gradients, thereby more closely resembling the conditions in the intact liver lobuli. METHODS: The bioreactor consists of a spirally wound nonwoven polyester matrix, i.e. a sheet-shaped, three-dimensional framework for hepatocyte immobilization and aggregation, and of integrated hydrophobic hollow-fiber membranes for decentralized oxygen supply and CO2 removal. Medium (plasma in vivo) was perfused through the extrafiber space and therefore in direct hepatocyte contact. Various parameters were assessed over a period of 4 days including galactose elimination, urea synthesis, lidocaine elimination, lactate/pyruvate ratios, amino acid metabolism, pH, the last day being reserved exclusively for determination of protein secretion. RESULTS: Microscopic examination of the hepatocytes revealed cytoarchitectural characteristics as found in vivo. The biochemical performance of the bioreactor remained stable over the investigated period. The urea synthesizing capacity of hepatocytes in the bioreactor was twice that of hepatocytes in monolayer cultures. Flow sensitive magnetic resonance imaging (MRI) revealed that the bioreactor construction ensured medium flow through all parts of the device irrespective of its size. CONCLUSIONS: The novel bioreactor showed encouraging efficiency. The device is easy to manufacture with scale-up to the liver mass required for possible short-term support of patients in hepatic failur

Effects of lithology and depth on the permeability of core samples from the Kola and KTB drill holes

Permeability measurements were conducted on intact core samples fromthe Kola drill hole in Russia and the KTB drill hole in Germany. Samples included granodiorite gneisses, basalts and amphibolites from depths up to 11 km. The tests were intended to determine the pressure sensitivity of permeability and to compare the effects of stress relief and thermal microcracking on the matrix permeability of different rock types and similar samples from different depths. The pore pressure Pp was fixed at the estimated in situ pressure assuming a normal hydrostatic gradient;the confining pressure Pc was varied to produce effective pressures(Pe= Pc-Pp) of 5 to 300 MPa. The permeability of the basaltic samples was the lowest andmost sensitive to pressure, ranging from 10-20 to 10-23m2 as effective pressure increased from 5 to only 60 MPa. In contrast, the granodiorite gneiss samples were more permeable and less sensitive to pressure, with permeability values rangingfrom 10-17 to 10-22 m2 as effective pressure increased to 300M Pa. Amphibolites displayed intermectiate behavior. There was an abundance of microfractures in the quartz-rich rocks, but a relative paucity of cracks in the mafic rocks, suggesting that the observed differences in permeability are based on rock type and depth, and that stress relief/thermal-cracking damage is correlated with quartz content. By applying the equivalent channel model of Walsh and Brace [1984] to the permeability data of the quartz-rich samples, we can estimate the closure pressure of the stress-relief cracks and thereby place bounds on the in situ effective pressure. This method may be usefulf or drill holes where the fluid pressure is not well constrained, such as at the Kola well. However, the use of crack closure to estimate in situ pressure was not appropriate for the basalt and amphibolite samples, because they are relatively crack-free in situ and remain so even after core retrieval. As a result, their permeability is near or below the measurable lower limit of our apparatus at the estimated in situ pressures of the rocks

3D crustal stress state of Germany according to a data-calibrated geomechanical model

The contemporary stress state in the upper crust is of great interest for geotechnical applications and basic research alike. However, our knowledge of the crustal stress field from the data perspective is limited. For Germany basically two datasets are available: orientations of the maximum horizontal stress (SHmax) and the stress regime as part of the World Stress Map (WSM) database as well as a complementary compilation of stress magnitude data of Germany and adjacent regions. However, these datasets only provide pointwise, incomplete and heterogeneous information of the 3D stress tensor. Here, we present a geomechanical–numerical model that provides a continuous description of the contemporary 3D crustal stress state on a regional scale for Germany. The model covers an area of about 1000×1250 km2 and extends to a depth of 100 km containing seven units, with specific material properties (density and elastic rock properties) and laterally varying thicknesses: a sedimentary unit, four different units of the upper crust, the lower crust and the lithospheric mantle. The model is calibrated by the two datasets to achieve a best-fit regarding the SHmax orientations and the minimum horizontal stress magnitudes (Shmin). The modeled orientations of SHmax are almost entirely within the uncertainties of the WSM data used and the Shmin magnitudes fit to various datasets well. Only the SHmax magnitudes show locally significant deviations, primarily indicating values that are too low in the lower part of the model. The model is open for further refinements regarding model geometry, e.g., additional layers with laterally varying material properties, and incorporation of future stress measurements. In addition, it can provide the initial stress state for local geomechanical models with a higher resolution