459 research outputs found

    The Impact of Financial Access on Firm Growth: Evidence from Ethiopian Grain Traders and Millers

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    Although both formal and informal financial institutions exist in developing economies, firms are often constrained by lack of access to financial services. Grain traders and millers in Ethiopia need a lot of finance to pay their suppliers (e.g. farmers) but it is not clear whether or not which sources of credit matter most for their growth and expansion. Using firm survey data collected for the purpose, we assessed access to and the impact of different sources of finance on growth of traders and millers in Ethiopia. Descriptive and econometric methods (e.g. ordered probit) were employed to address the issue. The results indicate that both formal and informal sources of credits are accessed by a small number of firms on a sporadic basis. With credit from commercial banks is mainly channeled to large businesses while microfinance institutions (MFIs) are designed to assist small and micro enterprises as part of a poverty alleviation strategy, medium firms such as most grain traders and millers have limited access to finance. Bank or MFI credit was found to have no impact on growth and expansion in the econometric analysis. Access to informal credit is also limited and largely used to meet short-term emergency cash requirements. Without improved and regular access to finance, grain traders and millers cannot make the necessary investment to provide effective marketing services for the transformation of agriculture.Key words: finance, financial access, Growth, Grain, Grain traders and millers, Ethiopi

    Moving Axle Load From MultiSpan Continuous Bridge

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    Effect of barley variety on feed intake, digestibility, body weight gain and carcass characteristics in fattening lambs

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    Twenty lambs (18 ± 0.22 kg initial weight) were blocked by weight and individually assigned into pens to evaluate the effects of barley straw variety on digestibility, growth performance and carcass characteristics. The following four treatments were tested: (1) a local barley straw (as control), (2) HB1963 (high grain and straw yields), (3) Traveller (high straw yielder), and (4) IBON174/03 (high grain yielder). A concentrate (50:50 wheat bran and noug seed cake) was offered constantly (300 DM g), whereas the straw was offered ad libitum. The digestibility trial lasted 22 days (15 days to adapt to dietary treatments and 7 days for sampling). The growth performance trial lasted 90 days. At the end, all of the lambs were slaughtered, and their carcasses were evaluated. The IBON174/03 variety had a higher (p < 0.05) intake of organic matter and crude protein, a higher dry matter and organic matter digestibility than the control, and a faster growth than the control. The feed-to-gain ratio was similar among treatments. The slaughter and empty body weights of lambs in the IBON174/03 group were higher than the control variety (p < 0.05). The present study showed that the feeding value of barley straw can differ substantially between varieties and therefore must be considered in the choice of a barley variety

    Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection.

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    BACKGROUND:Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants often target the P35S and/or TNOS DNA sequences. However, because the P35S promoter is derived from CaMV, these detection assays can yield false-positives from non-GM plants infected by this naturally-occurring virus. RESULTS:Here we report the development of an assay designed to distinguish CaMV-infected plants from GM plants in a single multiplexed quantitative PCR (qPCR) reaction. Following initial testing and optimization via PCR and singleplex-to-multiplex qPCR on both plasmid and plant DNA, TaqMan qPCR probes with different fluorescence wavelengths were designed to target actin (a positive-control plant gene), P35S, P3 (a CaMV-specific gene), and TNOS. We tested the specificity of our quadruplex qPCR assay using different DNA extracts from organic watercress and both organic and GM canola, all with and without CaMV infection, and by using commercial and industrial samples. The limit of detection (LOD) of each target was determined to be 1% for actin, 0.001% for P35S, and 0.01% for both P3 and TNOS. CONCLUSIONS:This assay was able to distinguish CaMV-infected plants from GM plants in a single multiplexed qPCR reaction for all samples tested in this study, suggesting that this protocol is broadly applicable and readily transferrable to any interested parties with a qPCR platform
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