"Jnking” atherosclerosis

Abstract.: Numerous studies in animal models established a key role of the C-jun N-terminal kinase (JNK) family (JNK1, JNK2 and JNK3) in numerous pathological conditions, including cancer, cardiac hypertrophy and failure, neurodegenerative disorders, diabetes, arthritis and asthma. A possible function of JNK in atherosclerosis remained uncertain since conclusions have mainly been based on in vitro studies investigating endothelial cell activation, T-effector cell differentiation and proliferation of vascular smooth muscle cells, all of which represent crucial cellular processes involved in atherosclerosis. However, recent experiments demonstrated that macrophage-restricted deletion of JNK2 was sufficient to efficiently reduce atherosclerosis in mice. Furthermore, it has been shown that JNK2 specifically promotes scavenger receptor A-mediated foam cell formation, an essential step during early atherogenesis, which occurs when vascular macrophages internalize modified lipoproteins. Thus, specific inhibition of JNK2 activity may emerge as a novel and promising therapeutic approach to attenuate atheroma formation in the future. In this review, we discuss JNK-dependent cellular and molecular mechanisms underlying atherosclerosi

Accesibilidad de las personas con discapacidad al Programa Escuela de Oficio “Hugo Rafael Chávez Frías”, Estelí, II semestre 2016

El propósito del estudio es valorar la percepción de los protagonistas sobre la accesibilidad a los programas de la Escuela de Oficio (HRCF), la identificación de las capacidades institucionales y de los docentes para la atención de las PCD; este análisis generó los elementos para la propuesta el cual tiene como objetivo facilitar el acceso de las PCD a los cursos que se imparten en la Escuela. Para darle una base contextual a la investigación planteada, se construyó un marco teórico, en el cual se sintetizan los aspectos fundamentales de la investigación, para ello se procedió a realizar una búsqueda en sitios web y libros de la biblioteca de FAREM-ESTELI Urania Zelaya. Posteriormente se procedió a construir los diferentes instrumentos de recolección de datos (entrevistas y encuestas) para la aplicación y análisis de los mismos, la información recolectada es fundamental para la construcción de la propuesta y así poderla adecuarla a las necesidades de las PCD que participan en los diferentes cursos de la Escuela de Oficio HRCF

Accesibilidad de las personas con discapacidad al Programa Escuela de Oficio “HRCF”, Estelí

Este artículo se enmarca en el estudio sobre la accesibilidad de las personas con discapacidad al Programa Escuela de Oficio “Hugo Rafael Chávez Frías”, Estelí, realizado durante el segundo semestre del 2016; en el cual se aborda la percepción de los protagonistas sobre la integración a los diversos cursos que se imparten en esa iniciativa educativa, así como las barreras que limitan el acceso, las capacidades institucionales y metodológicas de los docentes para la atención de las PCD. En el abordaje se profundizó la importancia de preparar al personal docente y para ello se generó una propuesta de formación metodológica que aspira a mejorar las condiciones educativas y el acceso. Para ello se integraron en el proceso investigativo a 35 protagonistas activos de los cursos con discapacidad y sin ella, 10 docentes, y 3 miembros de Juntas Directivas que atienden este sector. Los resultados contribuyen sobremanera a acercarnos al cumplimiento de políticas públicas orientadas a la educación inclusiva en el desarrollo de habilidades para la vida laboral encaminada a mejorar la calidad de vida

Narratives from the Online Frontier: A K-12 Student’s Experience in an Online Learning Environment

Despite a large increase in the number of students enrolled in online courses, published research on student experiences in these environments is minimal. This article reports the narrative analysis of a series of interviews conducted with a female student at a brick-and-mortar school enrolled in a single virtual school course. Her narratives describe a student who often struggled with the content in her online course and was reluctant to interact with her online teacher. When she interacted with people online, it was using text, because she was shy and the hardware often did not work. Darlene’s experiences, likely typical of many K-12 online students, highlight a system in need of better strategies for the design and delivery of its educational opportunities

Effect of higher salinities on growth and survival of pacific white shrimp, Litopenaeus vannamei (Boone, 1931)

The growth and survival of Litopenaeus vannamei post larvae was measured in controlled different salinities condition 35ppt (T1), 40ppt (T2), 45ppt (T3) and 50ppt (T4) were maintained. Group of Shrimp post larvae (weight 0.032 g ± 0.002) were stocked at a density of 35 nos. /aquarium in above salinity ranges. Animals were fed with commercial feed (35% Crude Protein) @ 5% of body weight four times a day. The results indicate that higher SGR was observed in T2 (1.99±0.08) followed by T1 (1.75±0.07), T3 (1.54±0.06) and T4 (1.49±0.17). Highest survival (100 %) was recorded in T1 followed by T2 (96.42%), T3 (94.99%) and T4 (74.21%). From the results of the present study it could be seen that higher salinity significantly reduced the growth and survival of L. vannamei but will also open study area of physiological adaption of animal at higher saline water in performance of organisms

Mechanistic insights into the transcriptional arrest in the presence of Double Strand Breaks

Double-strand breaks (DSBs) occur frequently in the genome during genome replication or by DNA damaging agents. DNA lesions affect fundamental DNA-dependent nuclear processes, such as replication and transcription. We have developed an experimental system where DSBs are induced at coding regions of RNA polymerase II transcribing genes. We have started to study the kinetics of RNA polymerase II transcription inhibition in the presence of DNA breaks. We observed that induction of the break led to transcription inhibition and the restoration of transcription closely followed the dynamics of the repair of breaks. We confirmed by chromatinimmunoprecipitation that the break induction led to displacement of RNA polymerase II affecting both the elongation and the initiation of transcription. Our results show that this is dependent on one of the major kinase in DNA damage repair called DNAPKcs. We also investigated the downstream steps of RNA polymerase II removal and we claimed that it was a multistep process involving additional kinases and ubiquitin ligases NEDD4 and CUL3. At the last step of break dependent transcriptional silencing the RNA polymerase II is targeted for proteasome dependent degradation. These data demonstrate that the DNA damage repair complexes and proteasomal system have a synergistic and active role in transcriptional silencing during the DSB repair by removing the RNA pol II from the transcribing region. We show here that DNA lesions occurring at transcribed regions cause a transient repression until the lesion is repaired. This is probably a cell defense mechanism to avoid production of truncated or mutated transcripts in essential genes whose alterations in their gene expression would endanger cell viability. Understudying the role of DNAPKcs, in preventing RNA pol II bypassing a DSB might be a key in avoiding the production of mutated transcripts that could lead to cancerous phenotypes

Tendencias de evolución de los servicios ecosistémicos brindados por los principales humedales vinculados a las aguas subterráneas en la República Dominicana

[ESP] La República Dominicana es un país rico en naturaleza y posee gran cantidad de humedales. El objetivo de este trabajo analizar la tendencia de algunos servicios ecosistémicos ofrecidos al bienestar humano por siete humedales vinculados a las aguas subterráneas. La metodología consistió en la recopilación y elaboración de información mediante tres fichas diseñadas ex profeso. Las principales conclusiones son que los servicios Culturales son los que mejoran en más humedales, tres servicios de Abastecimiento mejoran en varios casos, y respecto a los servicios de Regulación existe un gran desconocimiento debido a la escasez de información. [ENG] Dominican Republic is a country rich in nature and has lots of wetlands. The objective of this study is to analyze the trends of few ecosystem services to human wellbeing by seven wetlands linked to groundwater. The methodology consisted of compiling and elaborating information using three tabs designed expressly. The main conclusions are that Cultural services are improving in most wetlands, three Supply services improve in several cases, and respect to Regulatory service there is great ignorance due to the shortage of information.Centro Universitario de la Defensa. Escuela de Turismo de Cartagena. Escuela Técnica Superior de Ingeniería Industrial UPCT. Escuela Técnica Superior de Ingeniería de Telecomunicación (ETSIT). Escuela de Ingeniería de Caminos y Minas (EICM). Escuela de Arquitectura e Ingeniería de Edificación (ARQ&IDE). Parque Tecnológico de Fuente Álamo. Navantia. Campus Mare Nostrum. Estación Experimental Agroalimentaria Tomás Ferr

Role of E-Governance in Pakistan's Administration in Maintaining Public Trust

The study was based on the analysis of e-governance for administration in Pakistan related to public trust. The study was based on a quantitative approach and a literature review from a theoretical perspective. The quantitative approach was based on the survey from purposive sampling. The survey questionnaire was based on different variables based on the theoretical framework of public trust and the role of e-governance. The results from the survey have shown that e-governance is an emerging platform in Pakistan. However, it needs to work on accountability, responsiveness, clarity, transparency, laws, and regulations to become effective in maintaining public trust in maintaining Pakistan administration