1,210 research outputs found
Nanogranular MgB2 thin films on SiC buffered Si substrates prepared by in-situ method
MgB2 thin films were deposited on SiC buffered Si substrates by sequential
electron beam evaporation of B-Mg bilayer followed by in-situ annealing. The
application of a SiC buffer layer enables the maximum annealing temperature of
830 C. The Transmission Electron Microscopy analysis confirms the growth of a
nanogranular MgB2 film and the presence of a Mg2Si compound at the surface of
the film. The 150-200 nm thick films show a maximum zero resistance critical
temperature TC0 above 37 K and a critical current density JC ~ 106 A/cm2 at
11K.Comment: 7 pages, 6 figures, submitted to Applied Physics Letter
Nucleic Acids Res
Type II topoisomerases are essential enzymes that regulate DNA topology through a strand-passage mechanism. Some type II topoisomerases relax supercoils, unknot and decatenate DNA to below thermodynamic equilibrium. Several models of this non-equilibrium topology simplification phenomenon have been proposed. The kinetic proofreading (KPR) model postulates that strand passage requires a DNA-bound topoisomerase to collide twice in rapid succession with a second DNA segment, implying a quadratic relationship between DNA collision frequency and relaxation rate. To test this model, we used a single-molecule assay to measure the unlinking rate as a function of DNA collision frequency for Escherichia coli topoisomerase IV (topo IV) that displays efficient non-equilibrium topology simplification activity, and for E. coli topoisomerase III (topo III), a type IA topoisomerase that unlinks and unknots DNA to equilibrium levels. Contrary to the predictions of the KPR model, topo IV and topo III unlinking rates were linearly related to the DNA collision frequency. Furthermore, topo III exhibited decatenation activity comparable with that of topo IV, supporting proposed roles for topo III in DNA segregation. This study enables us to rule out the KPR model for non-equilibrium topology simplification. More generally, we establish an experimental approach to systematically control DNA collision frequency
Genotyping of the G1138A mutation of the FGFR3 gene in patients with achondroplasia using high-resolution melting analysis
[[abstract]]Objectives: The fibroblast growth factor receptor 3 gene (FGFR3) plays a critical role in cartilage growth-plate differentiation and bony development. It has been shown that 97% of patients with achondroplasia have a G to A transition mutation at position 1138 (c.1138 G>A) of codon 380 of the FGFR3 gene.
Design and methods: Exon 8 of the FGFR3 gene was analyzed in 40 patients with achondroplasia, as well as in 50 control individuals for the presence of the c.1138G>A variant using melting curve analysis with a high-resolution melting instrument (HR-1).
Results: The high-resolution melting curve analysis successfully genotyped the c.1138G>A mutation in exon 8 of the FGFR3 gene in all 40 patients with achondroplasia without the need of further assays. The technique had a sensitivity and specificity of 100%.
Conclusion: High-resolution melting analysis is a simple, rapid, and sensitive one tube assay for genotyping the FGFR3 gene. The technique is a low cost high-throughput FGFR3 screening assay. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved
Measurements of double-polarized compton scattering asymmetries and extraction of the proton spin polarizabilities
The spin polarizabilities of the nucleon describe how the spin of the nucleon responds to an incident polarized photon. The most model-independent way to extract the nucleon spin polarizabilities is through polarized Compton scattering. Double-polarized Compton scattering asymmetries on the proton were measured in the Î(1232) region using circularly polarized incident photons and a transversely polarized proton target at the Mainz Microtron. Fits to asymmetry data were performed using a dispersion model calculation and a baryon chiral perturbation theory calculation, and a separation of all four proton spin polarizabilities in the multipole basis was achieved. The analysis based on a dispersion model calculation yields ÎłE1E1=â3.5±1.2, ÎłM1M1=3.16±0.85, ÎłE1M2=â0.7±1.2, and ÎłM1E2=1.99±0.29, in units of 10â4ââfm4
Photoproduction of -pairs off protons and off neutrons
Total cross sections, angular distributions, and invariant-mass distributions
have been measured for the photoproduction of pairs off free
protons and off nucleons bound in the deuteron. The experiments were performed
at the MAMI accelerator facility in Mainz using the Glasgow photon tagging
spectrometer and the Crystal Ball/TAPS detector. The accelerator delivered
electron beams of 1508 and 1557~MeV, which produced bremsstrahlung in thin
radiator foils. The tagged photon beam covered energies up to 1400~MeV. The
data from the free proton target are in good agreement with previous
measurements and were only used to test the analysis procedures. The results
for differential cross sections (angular distributions and invariant-mass
distributions) for free and quasi-free protons are almost identical in shape,
but differ in absolute magnitude up to 15\%. Thus, moderate final-state
interaction effects are present. The data for quasi-free neutrons are similar
to the proton data in the second resonance region (final state invariant masses
up to 1550~MeV), where both reactions are dominated by the
decay. At higher energies,
angular and invariant-mass distributions are different. A simple analysis of
the shapes of the invariant-mass distributions in the third resonance region is
consistent with strong contributions of an decay
for the proton, while the reaction is dominated by a sequential decay via a
intermediate state for the neutron. The data are compared to
predictions from the Two-Pion-MAID model and the Bonn-Gatchina coupled channel
analysis.Comment: accepted for publication in Eur. Phys. J.
Photoproduction of Ï0-pairs off protons and off neutrons
Total cross sections, angular distributions, and invariant-mass distributions have been measured for the photoproduction of Ï0Ï0 pairs off free protons and off nucleons bound in the deuteron. The experiments were performed at the MAMI accelerator facility in Mainz using the Glasgow photon tagging spectrometer and the Crystal Ball/TAPS detector. The accelerator delivered electron beams of 1508 and 1557MeV, which produced bremsstrahlung in thin radiator foils. The tagged photon beam covered energies up to 1400MeV. The data from the free proton target are in good agreement with previous measurements and were only used to test the analysis procedures. The results for differential cross sections (angular distributions and invariant-mass distributions) for free and quasi-free protons are almost identical in shape, but differ in absolute magnitude up to 15%. Thus, moderate final-state interaction effects are present. The data for quasi-free neutrons are similar to the proton data in the second resonance region (final-state invariant masses up to â1550 MeV), where both reactions are dominated by the N(1520)3/2ââÎ(1232)3/2+Ï decay. At higher energies, angular and invariant-mass distributions are different. A simple analysis of the shapes of the invariant-mass distributions in the third resonance region is consistent with strong contributions of an NââNÏ decay for the proton, while the reaction is dominated by a sequential decay via a ÎÏ intermediate state for the neutron. The data are compared to predictions from the Two-Pion-MAID model and the Bonn-Gatchina coupled-channel analysis
Search for supersymmetric particles in scenarios with a gravitino LSP and stau NLSP
Sleptons, neutralinos and charginos were searched for in the context of
scenarios where the lightest supersymmetric particle is the gravitino. It was
assumed that the stau is the next-to-lightest supersymmetric particle. Data
collected with the DELPHI detector at a centre-of-mass energy near 189 GeV were
analysed combining the methods developed in previous searches at lower
energies. No evidence for the production of these supersymmetric particles was
found. Hence, limits were derived at 95% confidence level.Comment: 31 pages, 14 figure
Search for charginos in e+e- interactions at sqrt(s) = 189 GeV
An update of the searches for charginos and gravitinos is presented, based on
a data sample corresponding to the 158 pb^{-1} recorded by the DELPHI detector
in 1998, at a centre-of-mass energy of 189 GeV. No evidence for a signal was
found. The lower mass limits are 4-5 GeV/c^2 higher than those obtained at a
centre-of-mass energy of 183 GeV. The (\mu,M_2) MSSM domain excluded by
combining the chargino searches with neutralino searches at the Z resonance
implies a limit on the mass of the lightest neutralino which, for a heavy
sneutrino, is constrained to be above 31.0 GeV/c^2 for tan(beta) \geq 1.Comment: 22 pages, 8 figure
Mouse SPNS2 Functions as a Sphingosine-1-Phosphate Transporter in Vascular Endothelial Cells
Sphingosine-1-phosphate (S1P), a sphingolipid metabolite that is produced inside
the cells, regulates a variety of physiological and pathological responses via
S1P receptors (S1P1â5). Signal transduction between cells consists of
three steps; the synthesis of signaling molecules, their export to the
extracellular space and their recognition by receptors. An S1P concentration
gradient is essential for the migration of various cell types that express S1P
receptors, such as lymphocytes, pre-osteoclasts, cancer cells and endothelial
cells. To maintain this concentration gradient, plasma S1P concentration must be
at a higher level. However, little is known about the molecular mechanism by
which S1P is supplied to extracellular environments such as blood plasma. Here,
we show that SPNS2 functions as an S1P transporter in vascular endothelial cells
but not in erythrocytes and platelets. Moreover, the plasma S1P concentration of
SPNS2-deficient mice was reduced to approximately 60% of wild-type, and
SPNS2-deficient mice were lymphopenic. Our results demonstrate that SPNS2 is the
first physiological S1P transporter in mammals and is a key determinant of
lymphocyte egress from the thymus
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