42 research outputs found
Improved performance of PACE 2 with modified collection system in combination with probe competition assay for detecti
The Gen-Probe PACE 2 assay (GP) in combination with a modified collection
system was compared with cell culture (CC) for the detection of Chlamydia
trachomatis in urethral specimens from males. Analysis of discordant
results was performed by PCR. The modifications, i.e., application of a
more rigid swab type and a 50% reduction in the amount of transport
medium, were made to improve the sensitivity of the assay. By using the
modified GP on 302 urethral specimens from males, a sensitivity of 89.5%
and a specificity of 100% were determined. In addition, performance of a
probe competition assay on all GP samples with a result > 0.6 and < 1.0
times the cutoff factor (gray zone) detected three more true-positive
samples. The sensitivity of GP in combination with the probe competition
assay increased to 94.9%, with a specificity of 100%. This was identical
to the performance of CC. The modified GP offers a very sensitive and
specific alternative to CC
Evaluation of Clearview and Magic Lite tests, polymerase chain reaction, and cell culture for detection of Chlamydia trachomatis in urogenital specimens
The Clearview Chlamydia test (CV; Unipath Ltd., Bedford, United Kingdom),
the Magic Lite Chlamydia test (ML; CIBA Corning, Medfield, Mass.), a
polymerase chain reaction (PCR), and cell culture (CC) were evaluated for
detection of Chlamydia trachomatis in urogenital specimens. Specimens were
collected from 283 men and 724 women visiting the outpatient clinic for
Sexually Transmitted Diseases at the University Hospital Rotterdam,
Rotterdam, The Netherlands. ML, PCR, and CC were all performed on the same
sample to prevent swab-to-swab variability. CV was performed on a separate
sample. Analysis of discordant results was performed by application of the
following confirmatory assays: first, PCR on th
Fabrication and characterization of proton-exchanged waveguide on X-Cut LiNbO3
10.1109/IPGC.2008.47813582008 IEEE PhotonicsGlobal at Singapore, IPGC 2008
Hemorrhages in muscles of broiler chickens: The relationships among blood variables at various rearing temperature regimens.
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