Immunohistochemical labeling of Giardia trophozoites spp. in formalin fixed paraffin embedded tissues.

An immunoperoxidase method using rabbit anti human Giardia lamblia serum for the demonstration of giardia in paraffin embedded intestinal animal tissue is described. Specificity was tested against other protozoal parasites

A retrospective analysis of radiation therapy for the treament of feline vaccine-associated sarcoma

We retrospectively evaluated predictive prognostic factors in 73 cats with vaccineassociated sarcoma given postsurgical curative (n=46, most with clean margins) or coarse fractionated radiotherapy (n=27, most with either macroscopic disease or dirty margins). The former animals displayed a median survival of 43 months and a median progression free interval (PFI) of 37 months, the latter reached a median survival of 24 months and a median PFI of 10 months. In cats undergoing coarse fractionated therapy, factors predictive of a better outcome included lack of visible mass (n=10) as opposed to macroscopic disease (n=17, survival: 30 vs. 7 months, P=0.025; PFI: 20 vs. 4 months, P=0.01), adjuvant chemotherapy for gross disease (n=5/17, survival: 29 vs. 5 months, P=0.04) and a smaller number of surgeries preceding radiation therapy (Coeff=0.41,^P=0.03). The Ki67-index was not predictive for survival. We concluded that postsurgical curative and coarse fractionated radiotherapy are both effective legitimate options for managing vaccine-associated sarcomas