264 research outputs found
Assessment of the necessity of cooling of the Stirling engine expansion piston
Heat balance of the Stirling engine expansion piston is investigated. This balance considers heat flows that are applied to the expansion piston from outside parts, generated by friction in the piston-liner contact zone, and rejected to the cooling medium. The average heat transfer coefficient is obtained by means of division of a predicted value of the thermal conductance by cooled area of the expansion piston. Dimensionless variables are derived from an expression for determination of the thermal conductance, and the dimensionless chart is presented
Phantasmagorias in Uganda
Internship in Western Uganda during August of 2018https://deepblue.lib.umich.edu/bitstream/2027.42/148307/1/NathanStefanovskyLelaHickonbottom.pd
Zirconolite polytypes and murataite polysomes in matrices for the REE-actinide fraction of HLW
Electron backscatter diffraction (EBSD) has been used for more than 30 years for analyzing the structure of minerals and artificial substances. In recent times, EBSD has been widely applied for investigation of irradiated nuclear fuel and matrices for the immobilization of radioactive waste. The combination of EBSD and scanning electron microscopy (SEM/EDS) methods allows researchers to obtain simultaneously data on a specimen's local composition and structure. The article discusses the abilities of SEM/EDS and EBSD techniques to identify zirconolite polytype modifications and members of the polysomatic murataite-pyrochlore series in polyphase ceramic matrices, with simulations of Pu (Th) and the REE-actinide fraction (Nd) of high-level radioactive waste
The cellular abundance of the essential transcription termination factor TTF-I regulates ribosome biogenesis and is determined by MDM2 ubiquitinylation
The ARF tumour suppressor stabilizes p53 by negatively regulating the E3 ubiquitin ligase MDM2 to promote cell cycle arrest and cell death. However, ARF is also able to arrest cell proliferation by inhibiting ribosome biogenesis. In greater part this is achieved by targeting the transcription termination factor I (TTF-I) for nucleolar export, leading to an inhibition of both ribosomal RNA synthesis and processing. We now show that in the absence of ARF, TTF-I is ubiquitinylated by MDM2. MDM2 interacts directly with TTF-I and regulates its cellular abundance by targeting it for degradation by the proteasome. Enhanced TTF-I levels inhibit ribosome biogenesis by suppressing ribosomal RNA synthesis and processing, strongly suggesting that exact TTF-I levels are critical for efficient ribosome biogenesis. We further show that concomitant with its ability to displace TTF-I from the nucleolus, ARF inhibits MDM2 ubiquitinylation of TTF-I by competitively binding to a site overlapping the MDM2 interaction site. Thus, both the sub-nuclear localization and the abundance of TTF-I are key regulators of ribosome biogenesis
The Effect of Waste Loading and Glass Structural Factors on Structure and Chemical Durability of SB2 and SB4 SRS Waste Glasses -11397
ABSTRACT Glassy materials simulating vitrified high-Na/Fe (Sludge Batch 2 -SB2) and high-Na/Fe/Al (Sludge Batch 4 -SB4) Savannah River Site high level wastes (HLW) were produced in a resistive furnace and 236 and 418 mm inner diameter cold crucibles. The effect of waste loading (WL) and glass structural factors (degree of connectedness of glass network, metal oxides to boron oxide ratios) on chemical durability of glassy materials was studied
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Summary Of Cold Crucible Vitrification Tests Results With Savannah River Site High Level Waste Surrogates
The cold crucible inductive melting (CCIM) technology successfully applied for vitrification of low- and intermediate-level waste (LILW) at SIA Radon, Russia, was tested to be implemented for vitrification of high-level waste (HLW) stored at Savannah River Site, USA. Mixtures of Sludge Batch 2 (SB2) and 4 (SB4) waste surrogates and borosilicate frits as slurries were vitrified in bench- (236 mm inner diameter) and full-scale (418 mm inner diameter) cold crucibles. Various process conditions were tested and major process variables were determined. Melts were poured into 10L canisters and cooled to room temperature in air or in heat-insulated boxes by a regime similar to Canister Centerline Cooling (CCC) used at DWPF. The products with waste loading from ~40 to ~65 wt.% were investigated in details. The products contained 40 to 55 wt.% waste oxides were predominantly amorphous; at higher waste loadings (WL) spinel structure phases and nepheline were present. Normalized release values for Li, B, Na, and Si determined by PCT procedure remain lower than those from EA glass at waste loadings of up to 60 wt.%
Structural analysis and DNA binding of the HMG domains of the human mitochondrial transcription factor A
The mitochondrial transcription factor A (mtTFA) is central to assembly and initiation of the mitochondrial transcription complex. Human mtTFA (h-mtTFA) is a dual high mobility group box (HMGB) protein that binds site-specifically to the mitochondrial genome and demarcates the promoters for recruitment of h-mtTFB1, h-mtTFB2 and the mitochondrial RNA polymerase. The stoichiometry of h-mtTFA was found to be a monomer in the absence of DNA, whereas it formed a dimer in the complex with the light strand promoter (LSP) DNA. Each of the HMG boxes and the C-terminal tail were evaluated for their ability to bind to the LSP DNA. Removal of the C-terminal tail only slightly decreased nonsequence specific DNA binding, and box A, but not box B, was capable of binding to the LSP DNA. The X-ray crystal structure of h-mtTFA box B, at 1.35 Å resolution, revealed the features of a noncanonical HMG box. Interactions of box B with other regions of h-mtTFA were observed. Together, these results provide an explanation for the unusual DNA-binding properties of box B and suggest possible roles for this domain in transcription complex assembly
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