Systematics and morphological diversification of the Cordylidae (Squamata)

xiii, 327 pages : illustrations (chiefly color)The Cordylidae is a species poor but ecologically and morphologically diverse family of lizards endemic to sub-Saharan Africa. Although the majority of the cordylid species are strictly rupicolous (rock dwelling), the family also contains a diverse array of other specialist ecotypes. Due to this concentrated ecological and morphological variation, the family is an excellent system for studying macroevolutionary processes. Previous studies have hypothesized that the majority of diversity in the family evolved rapidly at the base of the viviparous subfamily (Cordylinae), possibly representing an adaptive radiation, but this has not been investigated empirically. The four central chapters of this thesis were designed to be synergistic, with each chapter providing information and helping develop hypotheses in the others. Chapter two places the phylogenetic relationships of the Cordylidae into a temporal framework using relaxed clock Bayesian analyses on an 11 gene, squamate-wide dataset with ten fossil calibrations. These analyses recover the viviparous cordylines as undergoing a period of rapid cladogenesis across the Oligocene-Miocene boundary, radiating into nine well-supported lineages, distributed around the edge of the great escarpment in South Africa. Chapters three and four focused on two different cordylid groups with complicated and unresolved taxomomic histories. By investigating species boundaries within the Smaug warreni species complex (chapter three) and Hemicordylus capensis (chapter four) I address whether the current taxonomy of the family accurately captures the actual species diversity of the family. A combination of phylogenetic, biogeographic and morphological analyses reveals new species within both groups. The fifth chapter attempts to test the hypothesis that the Cordylidae underwent an adaptive radiation following the evolution of viviparity. Morphological variation of the Cordylidae was represented through a combination of standardized ecomorphological measurements and novel, volumetric measurements of osteoderm distributions recovered using High-Resolution CT scanning. Ecological data for known cordylid localities was extracted from multiple commonly used climate layers. Morphological variation was correlated with microhabitat choice, but not with climatic variation. Existing analytical techniques and new methods reveal that the viviparous cordylids experienced an early burst of morphological diversification, while the oviparous subfamily, Platysaurinae, did not. These findings strongly support the hypothesis that the Cordylinae underwent a period of adaptive radiation during the Oligocene, possibly as a result of shifts in the climate and geological uplift of South Africa's Great Escarpment.Richard Gilder Graduate School at the American Museum of Natural History

A Preliminary Report on Unique Hail and Tornadic Storm Observations in Central Illinois and Eastern Indiana on 3 April 1974

published or submitted for publicationis peer reviewedOpe

Heat transfer between fluids in two phase flow

Thesis (B.S.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1961.MIT Institute Archives copy bound with: Rheology of blood plasma / Paul M. Cox, Jr., Henry L. Gabelnick. 1961.Includes bibliographical references (leaves 38-39).by Allan S. Douglas, Edward L. Tucker.B.S

Descriptive osteology and patterns of limb loss of the European limbless skink Ophiomorus punctatissimus (Squamata, Scincidae)

The limbless skink Ophiomorus punctatissimus is a cryptozoic species found in the Peloponnese region of Greece and on the Greek island Kythira. To provide the first thorough description of the cranial and postcranial osteology of this species, both disarticulated specimens and X-ray computed tomographies of wet-preserved specimens were examined in detail. Resulting from this, an anatomical atlas of this species is provided. Two separate considerations, an evolutionary and an ecomorphological one, are made based on the observed adaptations related to limb loss in this skink. The structure of the girdles shows a particular pattern of reduction: whereas the pelvic girdle is mostly vestigial, the pectoral girdle is instead well developed, with all the elements typical of limbed lizards except for the actual limbs. This led us to hypothesize an asynchronous pattern of limb reduction during the evolution of this species, in which the hindlimbs regressed earlier than the forelimbs. Furthermore, considerations based on overall body morphology, osteology and the structure of the inner ear led to the recognition of this species as a burrowing ecomorph. In contrast to the morphology normally displayed in this ecomorph, O. punctatissimus is characterized by the retention of autotomic vertebrae in its tail. This is consistent with the habitats in which it lives, where active burrowing would be difficult because of the hard, rocky terrain. Instead, this skink hides among rocks on the surface and is, therefore, subject to greater predation risk

A new species of Brachycephalus (Anura Brachycephalidae) from Santa Catarina, southern Brazil

A new species of Brachycephalus (Anura: Brachycephalidae) is described from the Atlantic Forest of northeastern state of Santa Catarina, southern Brazil. Nine specimens (eight adults and a juvenile) were collected from the leaf litter of montane forests 790–835 m above sea level (a.s.l.). The new species is a member of the pernix group by its bufoniform shape and the absence of dermal co-ossification and is distinguished from all its congeners by a combination of its general coloration (dorsal region of head, dorsum, legs, arms, and flanks light, brownish green to dark, olive green, with darker region in the middle of the dorsum and a white line along the vertebral column in most specimens) and by its smooth dorsum. The geographical distribution of the new species is highly reduced (extent of occurrence estimated as 25.04 ha, or possibly 34.37 ha). In addition, its habitat has experienced some level of degradation, raising concerns about the future conservation of the species. Preliminary density estimates suggest one calling individual every 3–4 m2 at 815–835 m a.s.l. and every 100 m2 at 790 m a.s.l. Together with the recently described B. boticario and B. fuscolineatus, the new species is among the southernmost species of Brachycephalus known to date

Image Analysis Techniques for the Automated Evaluation of Subaxial Subluxation in Cervical Spine X-Ray Images

Rheumatoid arthritis is a chronic inflammatory disease affecting synovial joints of the body, especially the hands and feet, spine, knees and hips. For many patients, the cervical spine is associated with rheumatoid arthritis. Subluxation is the abnormal movement of one of the bones that comprise a joint. In this research, image analysis techniques have been investigated for the recognition of cervical spine x-ray images with one or more instances of subaxial subluxation. Receiver operating characteristic curve results are presented, showing potential for subaxial subluxation discrimination on an image-by-image basis

Clinical and operative predictors of outcomes of carotid endarterectomy

ObjectiveThe net benefit for patients undergoing carotid endarterectomy is critically dependent on the risk of perioperative stroke and death. Information about risk factors can aid appropriate selection of patients and inform efforts to reduce complication rates. This study identifies the clinical, radiographic, surgical, and anesthesia variables that are independent predictors of deaths and stroke following carotid endarterectomy.MethodsA retrospective cohort study of patients undergoing carotid endarterectomy in 1997 and 1998 by 64 surgeons in 6 hospitals was performed (N = 1972). Detailed information on clinical, radiographic, surgical, anesthesia, and medical management variables and deaths or strokes within 30 days of surgery were abstracted from inpatient and outpatient records. Multivariate logistic regression models identified independent clinical characteristics and operative techniques associated with risk-adjusted rates of combined death and nonfatal stroke as well as all strokes.ResultsDeath or stroke occurred in 2.28% of patients without carotid symptoms, 2.93% of those with carotid transient ischemic attacks, and 7.11% of those with strokes (P < .0001). Three clinical factors increased the risk-adjusted odds of complications: stroke as the indication for surgery (odds ratio [OR], 2.84; 95% confidence interval [CI] = 1.55-5.20), presence of active coronary artery disease (OR, 3.58; 95% CI = 1.53-8.36), and contralateral carotid stenosis ≥50% (OR, 2.32; 95% CI = 1.33-4.02). Two surgical techniques reduced the risk-adjusted odds of death or stroke: use of local anesthesia (OR, 0.30; 95% CI = 0.16-0.58) and patch closure (OR, 0.43; 95% CI = 0.24-0.76).ConclusionsInformation about these risk factors may help physicians weigh the risks and benefits of carotid endarterectomy in individual patients. Two operative techniques (use of local anesthesia and patch closure) may lower the risk of death or stroke

Fatty acid oxidation is essential for egg production by the parasitic flatworm Schistosoma mansoni

Schistosomes, parasitic flatworms that cause the neglected tropical disease schistosomiasis, have been considered to have an entirely carbohydrate based metabolism, with glycolysis playing a dominant role in the adult parasites. However, we have discovered a close link between mitochondrial oxygen consumption by female schistosomes and their ability to produce eggs. We show that oxygen consumption rates (OCR) and egg production are significantly diminished by pharmacologic inhibition of carnitine palmitoyl transferase 1 (CPT1), which catalyzes a rate limiting step in fatty acid β-oxidation (FAO) and by genetic loss of function of acyl CoA synthetase, which complexes with CPT1 and activates long chain FA for use in FAO, and of acyl CoA dehydrogenase, which catalyzes the first step in FAO within mitochondria. Declines in OCR and egg production correlate with changes in a network of lipid droplets within cells in a specialized reproductive organ, the vitellarium. Our data point to the importance of regulated lipid stores and FAO for the compartmentalized process of egg production in schistosomes

Semicircular canal size constrains vestibular function in miniaturized frogs

Miniaturization has evolved repeatedly in frogs in the moist leaf litter environments of rainforests worldwide. Miniaturized frogs are among the world’s smallest vertebrates and exhibit an array of enigmatic features. One area where miniaturization has predictable consequences is the vestibular system, which acts as a gyroscope, providing sensory information about movement and orientation. We investigated the vestibular system of pumpkin toadlets, Brachycephalus (Anura: Brachycephalidae), a clade of miniaturized frogs from Brazil. The semicircular canals of miniaturized frogs are the smallest recorded for adult vertebrates, resulting in low sensitivity to angular acceleration due to insufficient displacement of endolymph. This translates into a lack of postural control during jumping in Brachycephalus and represents a physical constraint resulting from Poiseuille’s law, which governs movement of fluids within tubes

Structure of the Ambrosia Beetle (Coleoptera: Curculionidae) Mycangia Revealed Through Micro-Computed Tomography

Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae and Platypodinae) rely on a symbiosis with fungi for their nutrition. Symbiotic fungi are preserved and transported in specialized storage structures called mycangia. Although pivotal in the symbiosis, mycangia have been notoriously difficult to study, given their minute size and membranous structure. We compared the application of novel visualization methods for the study of mycangia, namely micro-computed tomography (micro-CT) and laser ablation tomography (LATscan) with traditional paraffin sectioning. Micro-CT scanning has shown the greatest promise in new organ discovery, while sectioning remains the only method with sufficient resolution for cellular visualization. All three common types of mycangia (oral, mesonotal, and pronotal) were successfully visualized and presented for different species of ambrosia beetles: Ambrosiodmus minor (Stebbing) 1909, Euplatypus compositus (Say) 1823, Premnobius cavipennis Eichhoff 1878, Scolytoplatypus raja Blandford 1893, Xylosandrus crassiusculus (Motschulsky) 1866 and X. amputatus (Blandford) 1894. A reconstruction of the mycangium and the surrounding musculature in X. amputatus is also presented. The advantages of micro-CT compared to the previously commonly used microtome sectioning include the easy visualization and recording of three-dimensional structures, their position in reference to other internal structures, the ability to distinguish natural aberrations from technical artifacts, and the unprecedented visualizations of the anatomic context of mycangia enabled by the integrated software