2,725 research outputs found
Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates
Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals. As a proof-of-principle, the ssOPM system is also used to acquire a dose response curve with the concentration of glucose in the culture medium being varied across 42 wells of a 96-well plate with the whole acquisition taking 9 min. The 3-D image data enable the FRET ratio to be measured as a function of distance from the surface of the spheroid. Overall, the results demonstrate the capability of the OPM system to measure spatio-temporal changes in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format
Polarization Diagnostics for Cool Core Cluster Emission Lines
The nature of the interaction between low-excitation gas filaments at ~104 K, seen in optical line emission, and diffuse X-ray emitting coronal gas at ~107 K in the centers of galaxy clusters remains a puzzle. The presence of a strong, empirical correlation between the two gas phases is indicative of a fundamental relationship between them, though as yet of undetermined cause. The cooler filaments, originally thought to have condensed from the hot gas, could also arise from a merger or the disturbance of cool circumnuclear gas by nuclear activity. Here, we have searched for intrinsic line emission polarization in cool core galaxy clusters as a diagnostic of fundamental transport processes. Drawing on developments in solar astrophysics, direct energetic particle impact induced polarization holds the promise to definitively determine the role of collisional processes such as thermal conduction in the ISM physics of galaxy clusters, while providing insight into other highly anisotropic excitation mechanisms such as shocks, intense radiation fields, and suprathermal particles. Under certain physical conditions, theoretical calculations predict of the order of 10% polarization. Our observations of the filaments in four nearby cool core clusters place stringent upper limits ( 0.1%) on the presence of emission line polarization, requiring that if thermal conduction is operative, the thermal gradients are not in the saturated regime. This limit is consistent with theoretical models of the thermal structure of filament interfacesPeer reviewe
Electronic structure of NiSSe across the phase transition
We report very highly resolved photoemission spectra of NiS(1-x)Se(x) across
the so-called metal-insulator transition as a function of temperature as well
as composition. The present results convincingly demonstrate that the low
temperature, antiferromagnetic phase is metallic, with a reduced density of
states at E. This decrease is possibly due to the opening of gaps along
specific directions in the Brillouin zone caused by the antiferromagnetic
ordering.Comment: Revtex, 4 pages, 3 postscript figure
Processing experiments on non-Czochralski silicon sheet
A program is described which supports and promotes the development of processing techniques which may be successfully and cost-effectively applied to low-cost sheets for solar cell fabrication. Results are reported in the areas of process technology, cell design, cell metallization, and production cost simulation
Anomalous behavior of spin wave resonances in Ga_{1-x}Mn_{x}As thin films
We report ferromagnetic and spin wave resonance absorption measurements on
high quality epitaxially grown Ga_{1-x}Mn_{x}As thin films. We find that these
films exhibit robust ferromagnetic long-range order, based on the fact that up
to seven resonances are detected at low temperatures, and the resonance
structure survives to temperatures close to the ferromagnetic transition. On
the other hand, we observe a spin wave dispersion which is linear in mode
number, in qualitative contrast with the quadratic dispersion expected for
homogeneous samples. We perform a detailed numerical analysis of the
experimental data and provide analytical calculations to demonstrate that such
a linear dispersion is incompatible with uniform magnetic parameters. Our
theoretical analysis of the ferromagnetic resonance data, combined with the
knowledge that strain-induced anisotropy is definitely present in these films,
suggests that a spatially dependent magnetic anisotropy is the most likely
reason behind the anomalous behavior observed.Comment: 9 pages, including 6 figure
Magma production and growth of the lava dome of the Soufriere Hills Volcano, Montserrat, West Indies: November 1995 to November 1997
Paradoxical evidence on ethnic inequities in child welfare: towards a research agenda
This paper aims to compare developments in theory and evidence about ethnic disparities in the USA with findings from the Child Welfare Inequalities Project in England with a view to identifying key issues for a future research agenda. It has a particular focus on the relevance of the concept of the Hispanic Paradox for disparate intervention rates between ethnic populations in England. Three key theoretical dimensions for explaining such disparities are identified and outlined: artefactual, demand and supply factors. Findings from the study in England are then introduced to explore the relevance of these dimensions in a data set of over 14,000 individual children who were either on child protection plans (with substantiated child abuse or neglect) or who were ‘looked after children: in out-of-home care, at the 31st March 2015. While some ethnic populations were experiencing much more difficult average socio-economic circumstances (SEC) than others (using deprivation scores for small neighbourhoods as a proxy measure of family SEC), such factors were only a partial explanation for differential intervention rates between ethnic groups. Overall, large differences in intervention rates were found between ethnic categories and sub-categories which also confounded simply attributing disparities to either cultural differences, such as family patterns, or to individual or institutionalised discrimination. The potential for cost saving if intervention rates could match those ethnic groups with the lowest levels of service use would be considerable. More research is needed to ensure that data is comprehensive, reliable and valid, that there is better understanding of how socio-economic factors affect service demand and what characteristics of different ethnic populations and different approaches to service provision contribute to differential intervention rates. Key elements of such a research agenda are identified
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