Contribuição dos media audiovisuais para a representação de eventos LGBTI+

Grande parte do conhecimento que possuímos deriva dos media, principalmente dos media audiovisuais, que têm moldado a forma como as audiências, nas últimas décadas, formam a sua opinião em relação a pessoas e eventos. A representação de determinados grupos minoritários nos media audiovisuais, além de ser um espelho da sociedade em geral, leva também à construção de ideias relacionadas com os mesmos, muitas vezes perpetuando estereótipos negativos e, consequentemente, comportamentos discriminatórios, perante as minorias, pela restante população. A população LGBTI+ (Lésbicas, Gays, Bissexuais, Transexuais, Intersexuais e outros), como grupo minoritário, tem também sido alvo de preconceito, e mal representada, nos órgãos de comunicação social ao longo de vários anos. O enquadramento e apresentação de eventos nos media afetam a forma como os recetores da informação interpretam os mesmos. Contudo, conscientes da representação de que são objeto, os ativistas LGBTI+ têm apostado numa relação de proximidade com os media, com o objetivo de melhorar a representação da comunidade. Será que os novos media têm contribuído para melhorar a divulgação e representação da comunidade, utilizando a internet para disseminar mais informação relacionada com esta? O estágio na multiplataforma online LGBTI+ Pois.pt proporcionou-me investigar a forma como funciona um órgão de comunicação direcionado para a população LGBTI+. Para obter um meio de comparação, decidi analisar todas as peças informativas presentes no site da TVI (Televisão Independente), “TVI Player”, relacionadas com a população LGBTI+ do ano 2019, e entender a forma como esta comunidade é representada neste media audiovisual generalista.Much of our knowledge comes from the media, especially audiovisual media, which has positioned audiences, in recent decades, in relation to people and events. The representation of certain minority groups in the audiovisual media, besides reflecting much of society in general, also leads to the construction of ideas related to them, often perpetuating negative stereotypes and, consequently, discriminatory behavior towards minorities by the rest of the population. The LGBTI+ population (Lesbian, Gay, Bisexual, Transgender, Intersex, and others), as a minority group, has also been a target of prejudice and misrepresentation in the media for several years. The framing and presentation of events in the media affects the way information is interpreted by the audience. However, aware of their representation, LGBTI+ activists have been focusing on a close relationship with the media in order to improve community representation, particularly in audiovisual media. New media have greatly contributed to improving community outreach and representation, by using internet to disseminate more information related to it. The internship, on the LGBTI+ online multiplatform “Pois.pt”, helped me to understand how a media organization for the LGBTI+ population works. For the sake of comparison, I decided to analyze all the informative segments on the TVI (Independent Television) website, “TVI Player”, related to the LGBTI+ population of the year 2019, and to understand how this community is represented in this generalist audiovisual media

Régime alimentaire de Dasyatis pastinaca et Myliobatis aquila (Elasmobranchii: Myliobatiformes) des Açores, Atlantique NE

This paper describes and discusses the feeding ecology of two stingray species that occur in the Azores: Dasyatis pastinaca and Myliobatis aquila. Both are common coastal species associated with sandy, muddy and rocky bottoms, from surface waters to depths of over 200 m. The diet described here is based on the stomach content analysis from 33 specimens (23 D. pastinaca, 10 M. aquila) collected by spearfishing between June and October 2010 at three different locations along the coast of Terceira Island, Azores. Food habits of D. pastinaca consist mainly of Decapod crustaceans (with a relevance for Pachygrapsus marmoratus) followed by other groups with high representativeness: Polychaeta and Mysidacea. The diet of M. aquila consists mainly of Calliostoma lusitanicum and Stramonita haemastoma. Both species from our sample proved to be generalist predators with different feeding habits. The diet of D. pastinaca is characterized by Crustacea (81% IRI) and that of M. aquila characterized by Mollusca (82% IRI).RÉSUMÉ: Cet article décrit et analyse l’écologie alimentaire de deux raies des Açores : Dasyatis pastinaca et Myliobatis aquila. Ce sont deux espèces côtières communes associées à des fonds sableux, vaseux et rocheux, se trouvant depuis les petits fonds jusqu’à plus de 200 m de profondeur. Le régime décrit ici est basé sur l’analyse du contenu stomacal de 33 spécimens (23 D. pastinaca, 10 M. aquila) récoltées en chasse sous-marine entre juin et octobre 2010 à trois endroits différents le long de la côte de l’île Terceira, aux Açores. Le régime alimentaire de D. pastinaca se compose principalement de crustacés décapodes (avec une préférence pour Pachygrapsus marmoratus), suivis par d’autres groupes fortement représentés: polychètes et mysidacés. Le régime de M. aquila se compose principalement de Calliostoma lusitanicum et Stramonita haemastoma. Les deux espèces sont des prédateurs généralistes avec des habitudes alimentaires différentes. Le régime alimentaire de D. pastinaca est caractérisé par les crustacés (81% IRI) et celui de M. aquila par les mollusques (82% IRI).info:eu-repo/semantics/publishedVersio

Exploring professional practice environments and organisational context factors affecting nurses’ adoption of evidence-based practice: a scoping review

This scoping review, conducted within the Joanna Briggs Institute (JBI) framework, analysed the recent literature (January 2018 to March 2023) addressing factors inherent to professional practice environments and organisational contexts influencing nurses’ adoption of evidence-based practice (EBP). This review included studies involving nurses regardless of sector, practice setting, and scope of practice. A systematic search was undertaken across the PubMed, Web of Science, CINAHL, and MEDLINE databases, as well as the EThOS, OATD, and RCAAP platforms. The extracted textual elements underwent a content analysis, resulting in a coding structure established through an inductive approach that categorised information into main categories and subcategories linked by similarity and thematic affinity. Forty-one studies were included, revealing four main categories of factors impacting EBP adoption by nurses: (1) organisational dynamics, (2) management and leadership, (3) teamwork and communication, and (4) resources and infrastructure. The study’s limitations acknowledge the subjective nature of categorisation, recognising potential variations based on individual perspectives despite adopting procedures to minimise the risk of bias. The results provide a substantial foundation for developing interventions to cultivate environments conducive to EBP adoption by nurses, thereby enhancing the integration of evidence into nurses’ professional practice contexts. This review was prospectively registered on the Open Science Framework (registration no. osf.io/e86qz).info:eu-repo/semantics/publishedVersio

Pituitary Apoplexy Following Systemic Anticoagulation

Pituitary apoplexy is a rare medico-surgical emergency that stems from an acute expansion of a pituitary adenoma from infarction or haemorrhage and where the treatment strategy is still controversial. Clinical presentation is highly variable and a high index of suspicion is needed to make the diagnosis. Furthermore, in less than half of cases, a precipitating event is identified. We report a case of a 74-year-old female who, after introduction of anticoagulation for pulmonary thromboembolism, presented with pituitary apoplexy heralded by acute adrenal insufficiency, headaches, visual symptoms and hypogonadotropic hypogonadism. Timely initiation of corticosteroids was crucial, and after stabilisation, a conservative treatment strategy was favoured with good long-term prognosis. Long-term follow-up of pituitary function also revealed new growth hormone deficiency

p53 Function Re-Establishment and Apoptosis Induction in Cervical Cancer Cells

Funding Information: Funding: This work was supported by the Foundation for Science and Technology (FCT), through funds from the State Budget, and by the European Regional Development Fund through the “Pro-grama Operacional Regional do Centro (Centro 2020)—Sistema de Apoio à Investigação Científica e Tecnológica—Programas Integrados de IC&DT” (Project Centro-01-0145-FEDER-000019—C4—Centro de Competências em Cloud Computing) and the project ref: UIDB/00709/2020. This work was also supported by national funds from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences—UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. D. Gomes also acknowledges the doctoral fellowship from FCT (ref: 2020.06792.BD). This work was also supported by the Ramalingaswami Fellowship (BT/RLF/Re-entry/64/2017), Department of Biotechnology, Govt. of India (V.K.). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Cervical cancer is the fourth leading cause of death in women worldwide, with 99% of cases associated with a human papillomavirus (HPV) infection. Given that HPV prophylactic vaccines do not exert a therapeutic effect in individuals previously infected, have low coverage of all HPV types, and have poor accessibility in developing countries, it is unlikely that HPV-associated cancers will be eradicated in the coming years. Therefore, there is an emerging need for the development of anti-HPV drugs. Considering HPV E6’s oncogenic role, this protein has been proposed as a relevant target for cancer treatment. In the present work, we employed in silico tools to discover potential E6 inhibitors, as well as biochemical and cellular assays to understand the action of selected compounds in HPV-positive cells (Caski and HeLa) vs. HPV-negative (C33A) and non-carcinogenic (NHEK) cell lines. In fact, by molecular docking and molecular dynamics simulations, we found three phenolic compounds able to dock in the E6AP binding pocket of the E6 protein. In particular, lucidin and taxifolin were able to inhibit E6-mediated p53 degradation, selectively reduce the viability, and induce apoptosis in HPV-positive cells. Altogether, our data can be relevant for discovering promising leads for the development of specific anti-HPV drugs.publishersversionpublishe

Prototype of an affordable pressure-controlled emergency mechanical ventilator for COVID-19

We present a viable prototype of a simple mechanical ventilator intended as a last resort to ventilate COVID-19 patients. The prototype implements the pressure-controlled continuous mandatory ventilation mode (PC-CMV) with settable breathing rates, inspiration/expiration time ratios and FiO2 modulation. Although safe, the design aims to minimize the use of technical components and those used are common in industry, so its construction may be possible in times of logistical shortage or disruption or in areas with reduced access to technical materials and at a moderate cost, affordable to lower income countries. Most of the device can be manufactured by modest technical means and construction plans are provided.Comment: This version differs from version 2 in that it includes toxicological and bio-safety tests and updated electronic

Comparative Effectiveness of COVID-19 Vaccines in Preventing Infections and Disease Progression from SARS-CoV-2 Omicron BA.5 and BA.2, Portugal

We estimated comparative primary and booster vaccine effectiveness (VE) of SARS-CoV-2 Omicron BA.5 and BA.2 lineages against infection and disease progression. During April-June 2022, we implemented a case-case and cohort study and classified lineages using whole-genome sequencing or spike gene target failure. For the case-case study, we estimated the adjusted odds ratios (aORs) of vaccination using a logistic regression. For the cohort study, we estimated VE against disease progression using a penalized logistic regression. We observed no reduced VE for primary (aOR 1.07 [95% CI 0.93-1.23]) or booster (aOR 0.96 [95% CI 0.84-1.09]) vaccination against BA.5 infection. Among BA.5 case-patients, booster VE against progression to hospitalization was lower than that among BA.2 case-patients (VE 77% [95% CI 49%-90%] vs. VE 93% [95% CI 86%-97%]). Although booster vaccination is less effective against BA.5 than against BA.2, it offers substantial protection against progression from BA.5 infection to severe disease.The acquisition of sequencing equipment and reagents used in this study by the Instituto Nacional de Saúde Doutor Ricardo Jorge was partially funded by the HERA project (grant no. 2021/PHF/23776) supported by the European Commission through the European Centre for Disease Control, and also partially funded by the GenomePT project (grant no. POCI-01-0145-FEDER-022184), supported by COMPETE 2020–Operational Programme for Competitiveness and Internationalisation, Lisboa Portugal Regional Operational Programme, Algarve Portugal Regional Operational, under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund, and by the Portuguese Science and Technology Foundation. Algarve Biomedical Center Laboratory received public funding through the Project ALG-D2-2021-06 Variants Screen in Southern Portugal– Monitoring Variants of Concern in Southern Portugal and the Portuguese Science and Technology Foundation national support through the Comprehensive Health Research Center (grant no. UIDP/04923/2020)info:eu-repo/semantics/publishedVersio

Comparative complete scheme and booster effectiveness of COVID‐19 vaccines in preventing SARS‐CoV‐2 infections with SARS‐CoV‐2 Omicron (BA.1) and Delta (B.1.617.2) variants: A case–case study based on electronic health records

Background: Information on vaccine effectiveness in a context of novel variants of concern (VOC) emergence is of key importance to inform public health policies. This study aimed to estimate a measure of comparative vaccine effectiveness between Omicron (BA.1) and Delta (B.1.617.2 and sub-lineages) VOC according to vaccination exposure (primary or booster). Methods: We developed a case–case study using data on RT-PCR SARS-CoV2-positive cases notified in Portugal during Weeks 49–51, 2021. To obtain measure of comparative vaccine effectiveness, we compared the odds of vaccination in Omicron cases versus Delta using logistic regression adjusted for age group, sex, region, week of diagnosis, and laboratory of origin. Results: Higher odds of vaccination were observed in cases infected by Omicron VOC compared with Delta VOC cases for both complete primary vaccination (odds ratio [OR] = 2.1; 95% confidence interval [CI]: 1.8 to 2.4) and booster dose (OR = 5.2; 95% CI: 3.1 to 8.8), equivalent to reduction of vaccine effectiveness from 44.7% and 92.8%, observed against infection with Delta, to 6.0% (95% CI: 29.2% to 12.7%) and 62.7% (95% CI: 35.7% to 77.9%), observed against infection with Omicron, for complete primary vaccination and booster dose, respectively. Conclusion: Consistent reduction in vaccine-induced protection against infection with Omicron was observed. Complete primary vaccination may not be protective against SARS-CoV-2 infection in regions where Omicron variant is dominant.Grant no. 2021/PHF/23776; POCI-01-0145-FEDER-022184; Project ALG-D2-2021-06info:eu-repo/semantics/publishedVersio

Vivência educativa de acadêmicos de medicina no minicurso “a abordagem eco-bio-social e a vigilância ativa na prevenção e controle do aedes aegypti”: um relato de experiência / Educational experience of medicine scholars in the “eco-bio-social approach and active surveillance in aedes aegypti prevention and control” short-course: an experience report

Crianças e adolescentes apresentam crescentemente coparticipação em ações no combate às arboviroses como Dengue, Zika e Chikungunya. As intervenções educativas com escolares praticadas em estados brasileiros e em grandes países, apresentam resultados positivos no conhecimento sobre o assunto e na mudança comportamental da comunidade. Estudos como a abordagem eco-bio-social evidenciam que o conhecimento ecológico, biológico e social dos determinantes de transmissão vetorial das arboviroses propõem uma ressignificação do processo saúde-doença e sugerem uma real mudança de hábitos individuais e coletivos. Este artigo visa relatar a vivência educativa de três acadêmicos de medicina da Universidade Estadual do Ceará no minicurso intitulado “A abordagem eco-bio-social e a vigilância ativa na prevenção e controle do Aedes aegypti” em uma escola municipal de Fortaleza. O minicurso foi ministrado por dois pesquisadores com auxílio dos três acadêmicos em atividades de exposição dialogada, estudo de caso, círculo de cultura e avaliação do encontro. Contou também com atividades interativas da Secretaria da Saúde do Estado do Ceará (SESA) na apresentação de maquetes e distribuição de materiais informativos. Despertou-se a curiosidade, a atenção de seu público-alvo e da equipe condutora; permitiu-se a discussão sobre a ressignificação de conceitos relacionados ao processo saúde-doença, agindo como uma via de acesso à toda a comunidade, uma vez que os alunos foram instruídos a orientar ações de combate ao vetor no seu domicílio. O ambiente interativo, igualitário e lúdico estimulou o diálogo. Os escolares expuseram vivências individuais e questionamentos. A transdiciplinaridade e a integração entre o conhecimento individual e coletivo, que foram colocadas em discussão, mostraram uma relação construtiva e positiva para a formação dos escolares, como também para a equipe condutora do minicurso. Construiu-se coletivamente um aprendizado crítico, sendo uma experiência enriquecedora com dialogicidade entre todos os envolvidos.

Complete axillary dissection without drainage for the surgical treatment of breast cancer: a randomized clinical trial

OBJECTIVE: This randomized clinical trial evaluated the possibility of not draining the axilla following axillary dissection. METHODS: The study included 240 breast cancer patients who underwent axillary dissection as part of conservative treatment. The patients were divided into two groups depending on whether or not they were subjected to axillary drainage. ClinicalTrials.gov: NCT01267552. RESULTS: The median volume of fluid aspirated was significantly lower in the axillary drainage group (0.00 ml; 0.00 - 270.00) compared to the no drain group (522.50 ml; 130.00 - 1148.75). The median number of aspirations performed during conservative breast cancer treatment was significantly lower in the drainage group (0.5; 0.0 - 4.0) compared to the no drain group (5.0; 3.0 - 7.0). The total volume of serous fluid produced (the volume of fluid obtained from drainage added to the volume of aspirated fluid) was similar in the two groups. Regarding complications, two cases (2.4%) of wound dehiscence occurred in the drainage group compared to 13 cases (13.5%) in the group in which drainage was not performed, with this difference being statistically significant. Rates of infection, necrosis and hematoma were similar in both groups. CONCLUSION: Safety rates were similar in both study groups; hence, axillary dissection can feasibly be performed without drainage. However, more needle aspirations could be required, and there could be more cases of wound dehiscence in patients who do not undergo auxiliary drainage