Imaging Young Stellar Objects with VLTi/PIONIER

OHP 2013: International Colloquium at Haute-Provence Observatory, France, 23-27 September 2013 - Improving the performances of current optical interferometers and future designsThis is the final version of the paper. Available from the publisher via the URL in this record.Optical interferometry imaging is designed to help us to reveal complex astronomical sources without a prior model. Among these complex objects are the young stars and their environments, which have a typical morphology with a point-like source, surrounded by circumstellar material with unknown morphology. To image them, we have developed a numerical method that removes completely the stellar point source and reconstructs the rest of the image, using the differences in the spectral behavior between the star and its circumstellar material. We aim to reveal the first Astronomical Units of these objects where many physical phenomena could interplay: the dust sublimation causing a puffed-up inner rim, a dusty halo, a dusty wind or an inner gaseous component. To investigate more deeply these regions, we carried out the first Large Program survey of HAeBe stars with two main goals: statistics on the geometry of these objects at the first astronomical unit scale and imaging their very close environment. The images reveal the environment, which is not polluted by the star and allows us to derivethe best fit for the flux ratio and the spectral slope. We present the first images from this survey and the application of the imaging method on other astronomical objects.This work is supported by the French ANR POLCA project (Processing of pOLychromatic interferometriC data for Astrophysics, ANR-10-BLAN-0511

The 2014 interferometric imaging beauty contest

This is the final version of the article. Available from SPIE via the DOI in this record.From Conference Volume 9146: Optical and Infrared Interferometry IV. Jayadev K. Rajagopal; Michelle J. Creech-Eakman; Fabien Malbet. Montréal, Quebec, Canada, June 22, 2014Here we present the results of the 6th biennial optical interferometry imaging beauty contest. Taking advantage of a unique opportunity, the red supergiant VY CMa and the Mira variable R Car were observed in the astronomical H-band with three 4-telescope configurations of the VLTI-AT array using the PIONIER instrument. The community was invited to participate in the subsequent image reconstruction and interpretation phases of the project. Ten groups submitted entries to the beauty contest, and we found reasonable consistency between images obtained from independent workers using quite different algorithms. We also found that significant differences existed between the submitted images, much greater than in past beauty contests that were all based on simulated data. A novel crowd-sourcing" method allowed consensus median images to be constructed, filtering likely artifacts and retaining real features." We definitively detect strong spots on the surfaces of both stars as well as distinct circumstellar shells of emission (likely water/CO) around R Car. In a close contest, Joel Sanchez (IAA-CSIC/Spain) was named the winner of the 2014 interferometric imaging beauty contest. This process has shown that new comers" can use publicly-available imaging software to interpret VLTI/PIONIER imaging data, as long as sufficient observations are taken to have complete uv coverage { a luxury that is often missing. We urge proposers to request adequate observing nights to collect sufficient data for imaging and for time allocation committees to recognise the importance of uv coverage for reliable interpretation of interferometric data. We believe that the result of the proposed broad international project will contribute to inspiring trust in the image reconstruction processes in optical interferometry

The 10th VLTI School of Interferometry: Premiering a Fully Online Format

International audienceVery Large Telescope Inerferometer (VLTI) schools have nearly a 20-year history and have trained a significant fraction of today's optical interferometrists who use high-angular-resolution techniques on a regular basis. Very early in the development of the VLTI, training was identified by the community as a necessary tool, as the expertise in optical long-baseline interferometry was limited to a few groups in France and Germany (in those early years the UK was not an ESO member state). The first VLTI school took place in Les Houches, France, in 2002 and since then VLTI schools have been organised in several locations (France, Germany, Hungary, Poland, Portugal) roughly every two years, the previous one being held in 2018 in Lisbon. The VLTI schools are funded and coordinated through the European Interferometry Initiative (Eii)

Renal artery stenosis evaluation: diagnostic performance of gadobenate dimeglumine-enhanced MR angiography--comparison with DSA

PURPOSE: To prospectively determine diagnostic performance and safety of contrast material-enhanced (CE) magnetic resonance (MR) angiography with 0.1 mmol per kilogram of body weight gadobenate dimeglumine for depiction of significant steno-occlusive disease (> or =51% stenosis) of renal arteries, with digital subtraction angiography (DSA) as reference standard. MATERIALS AND METHODS: This multicenter study was approved by local institutional review boards; all patients provided written informed consent. Patient enrollment and examination at centers in the United States complied with HIPAA. Two hundred ninety-three patients (154 men, 139 women; mean age, 61.0 years) with severe hypertension (82.2%), progressive renal failure (11.3%), and suspected renal artery stenosis (6.5%) underwent CE MR angiography with three-dimensional spoiled gradient-echo sequences after administration of 0.1 mmol/kg gadobenate dimeglumine at 2 mL/sec. Anteroposterior and oblique DSA was performed in 268 (91.5%) patients. Three independent blinded reviewers evaluated CE MR angiographic images. Sensitivity, specificity, and accuracy of CE MR angiography for detection of significant steno-occlusive disease (> or =51% vessel lumen narrowing) were determined at segment (main renal artery) and patient levels. Positive and negative predictive values and positive and negative likelihood ratios were determined. Interobserver agreement was analyzed with generalized kappa statistics. A safety evaluation (clinical examination, electrocardiogram, blood and urine analysis, monitoring for adverse events) was performed. RESULTS: Of 268 patients, 178 who were evaluated with MR angiography and DSA had significant steno-occlusive disease of renal arteries at DSA. Sensitivity, specificity, and accuracy of CE MR angiography for detection of 51% or greater stenosis or occlusion were 60.1%-84.1%, 89.4%-94.7%, and 80.4%-86.9%, respectively, at segment level. Similar values were obtained for predictive values and for patient-level analyses. Few CE MR angiographic examinations (1.9%-2.8%) were technically inadequate. Interobserver agreement for detection of significant steno-occlusive disease was good (79.9% agreement; kappa = 0.69). No safety concerns were noted. CONCLUSION: CE MR angiography performed with 0.1 mmol/kg gadobenate dimeglumine, compared with DSA, is safe and provides good sensitivity, specificity, and accuracy for detection of significant renal artery steno-occlusive disease

Science cases for a visible interferometer

High spatial resolution is the key for the understanding various astrophysical phenomena. But even with the future E-ELT, single dish instruments are limited to a spatial resolution of about 4 mas in the visible. For the closest objects within our Galaxy most of the stellar photosphere remains smaller than 1 mas. With the success of long baseline interferometry these limitations were soom overcome. Today low and high resolution interferometric instruments on the VLTI and CHARA offer an immense range of astrophysical studies. Combining more telescopes and moving to visible wavelengths broadens the science cases even more. With the idea of developing strong science cases for a future visible interferometer, we organized a science group around the following topics: pre-main sequence and main sequence stars, fundamental parameters, asteroseismology and classical pulsating stars, evolved stars, massive stars, active galactic nuclei (AGNs) and imaging techniques. A meeting was organized on the 15th and 16th of January, 2015 in Nice with the support of the Action Specific in Haute Resolution Angulaire (ASHRA), the Programme National en Physique Stellaire (PNPS), the Lagrange Laboratory and the Observatoire de la Cote d'Azur, in order to present these cases and to discuss them further for future visible interferometers. This White Paper presents the outcome of the exchanges. This book is dedicated to the memory of our colleague Olivier Chesneau who passed away at the age of 41

Dynamic changes in gene expression in vivo predict prognosis of tamoxifen-treated patients with breast cancer

Introduction: Tamoxifen is the most widely prescribed anti-estrogen treatment for patients with estrogen receptor (ER)-positive breast cancer. However, there is still a need for biomarkers that reliably predict endocrine sensitivity in breast cancers and these may well be expressed in a dynamic manner. Methods: In this study we assessed gene expression changes at multiple time points (days 1, 2, 4, 7, 14) after tamoxifen treatment in the ER-positive ZR-75-1 xenograft model that displays significant changes in apoptosis, proliferation and angiogenesis within 2 days of therapy. Results: Hierarchical clustering identified six time-related gene expression patterns, which separated into three groups: two with early/transient responses, two with continuous/late responses and two with variable response patterns. The early/transient response represented reductions in many genes that are involved in cell cycle and proliferation (e.g. BUB1B, CCNA2, CDKN3, MKI67, UBE2C), whereas the continuous/late changed genes represented the more classical estrogen response genes (e.g. TFF1, TFF3, IGFBP5). Genes and the proteins they encode were confirmed to have similar temporal patterns of expression in vitro and in vivo and correlated with reduction in tumour volume in primary breast cancer. The profiles of genes that were most differentially expressed on days 2, 4 and 7 following treatment were able to predict prognosis, whereas those most changed on days 1 and 14 were not, in four tamoxifen treated datasets representing a total of 404 patients. Conclusions: Both early/transient/proliferation response genes and continuous/late/estrogen-response genes are able to predict prognosis of primary breast tumours in a dynamic manner. Temporal expression of therapy-response genes is clearly an important factor in characterising the response to endocrine therapy in breast tumours which has significant implications for the timing of biopsies in neoadjuvant biomarker studies.Publisher PDFPeer reviewe

Internal fixation treatments for intertrochanteric fracture: A systematic review and meta-Analysis of randomized evidence

The relative effects of internal fixation strategies for intertrochanteric fracture after operation remain uncertain. We conducted a systematic review and meta-Analysis of randomized controlled trials (RCTs) to address this important issue. We searched PubMed, EMBASE and CENTRAL for RCTs that compared different internal fixation implants in patients with intertrochanteric fracture at 6-month follow-up or longer. We ultimately included 43 trials enrolling 6911 patients; most trials were small in sample sizes and events. Their risk of bias was generally unclear due to insufficient reporting. Because of these, no statistically significant differences were present from most of the comparisons across all the outcomes, and no definitive conclusions can be made. However, a number of trials compared two commonly used internal fixation strategies, gamma nail (GN) and sliding hip screw (SHS). There is good evidence suggesting that, compared to SHS, GN may increase the risk of cut out (OR = 1.87, 95% CI, 1.08 to 3.21), re-operation (OR = 1.61, 95% CI, 1.02 to 2.53), intra-operative (OR = 3.14, 95% CI, 1.34 to 7.35) and later fractures (OR = 3.67, 95% CI, 1.37 to 9.83). Future randomized trials or observational studies that are carefully designed and conducted are warranted to establish the effects of alternative internal fixation strategies for intertrochanteric fracture

A family portrait of disk inner rims around Herbig Ae/Be stars. Hunting for warps, rings, self shadowing, and misalignments in the inner astronomical units

Context. The innermost astronomical unit (au) in protoplanetary disks is a key region for stellar and planet formation, as exoplanet searches have shown a large occurrence of close-in planets that are located within the first au around their host star. Aims: We aim to reveal the morphology of the disk inner rim using near-infrared interferometric observations with milli-arcsecond resolution provided by near-infrared multitelescope interferometry. Methods: We provide model-independent reconstructed images of 15 objects selected from the Herbig AeBe survey carried out with PIONIER at the Very Large Telescope Interferometer, using the semi-parametric approach for image reconstruction of chromatic objects. We propose a set of methods to reconstruct and analyze the images in a consistent way. Results: We find that 40% of the systems (6/15) are centrosymmetric at the angular resolution of the observations. For the rest of the objects, we find evidence for asymmetric emission due to moderate-to-strong inclination of a disk-like structure for ~30% of the objects (5/15) and noncentrosymmetric morphology due to a nonaxisymmetric and possibly variable environment (4/15, ~27%). Among the systems with a disk-like structure, 20% (3/15) show a resolved dust-free cavity. Finally, we do not detect extended emission beyond the inner rim. Conclusions: The image reconstruction process is a powerful tool to reveal complex disk inner rim morphologies, which is complementary to the fit of geometrical models. At the angular resolution reached by near-infrared interferometric observations, most of the images are compatible with a centrally peaked emission (no cavity). For the most resolved targets, image reconstruction reveals morphologies that cannot be reproduced by generic parametric models (e.g., perturbed inner rims or complex brightness distributions). Moreover, the nonaxisymmetric disks show that the spatial resolution probed by optical interferometers makes the observations of the near-infrared emission (inside a few au) sensitive to temporal evolution with a time-scale down to a few weeks. The evidence of nonaxisymmetric emission that cannot be explained by simple inclination and radiative transfer effects requires alternative explanations, such as a warping of the inner disks. Interferometric observations can therefore be used to follow the evolution of the asymmetry of those disks at an au or sub-au scale

Identification of target genes for wild type and truncated HMGA2 in mesenchymal stem-like cells

Background The HMGA2 gene, coding for an architectural transcription factor involved in mesenchymal embryogenesis, is frequently deranged by translocation and/or amplification in mesenchymal tumours, generally leading to over-expression of shortened transcripts and a truncated protein. Methods To identify pathways that are affected by sarcoma-associated variants of HMGA2, we have over-expressed wild type and truncated HMGA2 protein in an immortalized mesenchymal stem-like cell (MSC) line, and investigated the localisation of these proteins and their effects on differentiation and gene expression patterns. Results Over-expression of both transgenes blocked adipogenic differentiation of these cells, and microarray analysis revealed clear changes in gene expression patterns, more pronounced for the truncated protein. Most of the genes that showed altered expression in the HMGA2-overexpressing cells fell into the group of NF-κB-target genes, suggesting a central role for HMGA2 in this pathway. Of particular interest was the pronounced up-regulation of SSX1, already implicated in mesenchymal oncogenesis and stem cell functions, only in cells expressing the truncated protein. Furthermore, over-expression of both HMGA2 forms was associated with a strong repression of the epithelial marker CD24, consistent with the reported low level of CD24 in cancer stem cells. Conclusions We conclude that the c-terminal part of HMGA2 has important functions at least in mesenchymal cells, and the changes in gene expression resulting from overexpressing a protein lacking this domain may add to the malignant potential of sarcomas

Sparsity and Compressed Sensing in Inverse Problems

This chapter is concerned with two important topics in the context of sparse recovery in inverse and ill-posed problems. In first part we elaborate condi-tions for exact recovery. In particular, we describe how both `1-minimization and matching pursuit methods can be used to regularize ill-posed problems and more-over, state conditions which guarantee exact recovery of the support in the sparse case. The focus of the second part is on the incomplete data scenario. We discuss ex-tensions of compressed sensing for specific infinite dimensional ill-posed measure-ment regimes. We are able to establish recovery error estimates when adequately relating the isometry constant of the sensing operator, the ill-posedness of the un-derlying model operator and the regularization parameter. Finally, we very briefly sketch how projected steepest descent iterations can be applied to retrieve the sparse solution