Effects of central and peripheral fatigue on impact characteristics during running

Fatigue and impact can represent an injury risk factor during running. The objective of this study was to compare the impact transmission along the locomotor system between the central and peripheral fatigued states during running. Tibial and head acceleration as well as shock attenuation in the time- and frequency-domain were analyzed during 2-min of treadmill running in the pre- and post-fatigue state in eighteen male popular runners (N = 18). The impact transmission was measured before and after a 30-min central fatigue protocol on the treadmill or a peripheral fatigue protocol in the quadricep and hamstring muscles using an isokinetic dynamometer. The time-domain acceleration variables were not modified either by peripheral or central fatigue (p > 0.05). Nevertheless, central fatigue increased the maximum (p = 0.006) and total (p = 0.007) signal power magnitude in the high-frequency range in the tibia, and the attenuation variable in the low- (p = 0.048) and high-frequency area (p = 0.000), while peripheral fatigue did not cause any modifications in the frequency-domain variables (p > 0.05). Furthermore, the attenuation in the low (p = 0.000)- and high-frequency area was higher with central fatigue than peripheral fatigue (p = 0.003). The results demonstrate that central fatigue increases the severity of impact during running as well as the attenuation of low and high components

Evaluation of impact-shock on gait after the implementation of two different training programs in older adults

Gait is negatively affected with increasing age. It is widely accepted that training produces physical-functional improvements in older adults, which can be assessed with numerous physical-functional tests. However, very few studies have been carried out using accelerometry to analyse the training effect on kinetic and kinematic variables in older adults, and there is no one that investigate the effects of two different training programs. Therefore, the aim of this study is to analyse the effects of an interval-walking program and a multicomponent program on the acceleration impacts, shock attenuation, step-length, stride frequency, and gait speed in older adults. Methods: 23 participants were divided into multicomponent training group [n = 12, 7 female, 71.58 (4.56) years] and interval-walking group [n = 11, 6 female, 69.64 (3.56) years]. We evaluated the participants using three triaxial accelerometers, placing one on the distal end of each tibia and one on the forehead. Findings: After 14 weeks' of training, the maximum acceleration values both for the head accelerometer and for the non-dominant tibia, as well as the attenuation in the same leg, increased in the multicomponent training group. The maximum acceleration values for the head and the stride frequency also increased in the intervalwalking group. Lower limb strength improved in both groups. Interpretation: Given the benefits we found for each of these programs, we encourage their consideration when planning older adults training programs and suggest that multicomponent programs should be introduced prior to the start of walking-based programs

Validity and Reliability of an Instrumented Treadmill with an Accelerometry System for Assessment of Spatio-Temporal Parameters and Impact Transmission

Running retraining programs focused on concurrent feedback of acceleration impacts have been demonstrated to be a good strategy to reduce running-related injuries (RRI), as well as to improve running economy and reduce acceleration impacts and injury running incidence. Traditionally, impacts have been registered by mean of accelerometers attached directly to the athletes, which is inaccessible to the entire population, because it requires laboratory conditions. This study investigated the validity and reliability of a new device integrated directly into the treadmill, compared to a traditional acceleration impact system. Thirty healthy athletes with no history of RRI were tested on two separate days over the instrumented treadmill (AccTrea) and simultaneously with an acceleration impact system attached to the participant (AccAthl). AccTrea was demonstrated to be a valid and reliable tool for measuring spatio-temporal parameters like step length (validity intraclass correlation coefficient (ICC) = 0.94; reliability ICC = 0.92), step time (validity ICC = 0.95; reliability ICC = 0.96), and step frequency (validity ICC = 0.95; reliability ICC = 0.96) during running. Peak acceleration impact variables showed a high reliability for the left (reliability ICC = 0.88) and right leg (reliability ICC = 0.85), and peak impact asymmetry showed a modest validity (ICC = 0.55). These results indicated that the AccTrea system is a valid and reliable way to assess spatio-temporal variables, and a reliable tool for measuring acceleration impacts during running.This research was funded by Bodytone International Sport, S.L., grant number CFE-BODYTONE-03-18

Modification of Angular Kinematics and Spatiotemporal Parameters during Running after Central and Peripheral Fatigue

Fatigue causes kinematics modifications during running, and it could be related to injuries. The aim was to identify and compare the effects of central and peripheral fatigue on angular kinematics and spatiotemporal parameters during running. Angular kinematics and spatiotemporal parameters were evaluated using an infrared motion capture system and were registered during 2 min treadmill running in pre- and post-fatigue states in eighteen male recreational runners. Central fatigue was induced by a 30 min running fatigue protocol on a treadmill, while peripheral fatigue in quadriceps and hamstrings muscles was induced by an isokinetic dynamometer fatigue protocol. Central fatigue increased the anterior shank oscillation during the initial contact, knee flexion during the maximum absorption, posterior shank oscillation during propulsion, and stance time (p < 0.05). Peripheral fatigue decreased ankle dorsiflexion during initial contact and increased knee flexion and posterior shank oscillation during propulsion (p < 0.05). Moreover, central fatigue increased to a greater extent the hip and knee flexion and ankle dorsiflexion during initial contact and maximum absorption as well as stance time and propulsion time (p < 0.05). These results suggested that central fatigue causes greater increases in the range of movements during the midstance than peripheral fatigue

Equilibrium, Kinetic, and Computational Studies on the Formation of Cu2+ and Zn2+ Complexes with an Indazole-Containing Azamacrocyclic Scorpiand: Evidence for Metal-Induced Tautomerism

Cu2+ and Zn2+ coordination chemistry of a new member of the family of scorpiand-like macrocyclic ligands derived from tris(2-aminoethyl)amine (tren) is reported. The new ligand (L1) contains in its pendant arm not only the amine group derived from tren but also a 6-indazole ring. Potentiometric studies allow the determination of four protonation constants. UV−vis and fluorescence data support that the last protonation step occurs on the indazole group. Equilibrium measurements in the presence of Cu2+ and Zn2+ reveal the formation of stable [ML1]2+, [MHL1]3+, and [ML1(OH)]+ complexes. Kinetic studies on the acid-promoted decomposition of the metal complexes were carried out using both absorbance and fluorescence detection. For Zn2+, both types of detection led to the same results. The experiments suggest that [ZnL1]2+ protonates upon addition of an acid excess to form [ZnHL1]3+ within the mixing time of the stopped-flow instrument, which then decomposes with a first-order dependence on the acid concentration. The kinetic behavior is more complex in the case of Cu2+. Both [CuL1]2+ and [CuHL1]3+ show similar absorption spectra and convert within the mixing time to a new intermediate species with a band at 750 nm, the process being reverted by addition of base. The intermediate then decomposes with a secondorder dependence on the acid concentration. However, kinetic experiments with fluorescence detection showed the existence of an additional faster step. With the help of DFT calculations, an interpretation is proposed in which protonation of [CuL1]2+ to form [CuHL1]3+ would involve dissociation of the tren-based NH group in the pendant arm and coordination of a 2H-indazole group. Further protonation would lead to dissociation of coordinated indazole, which then will convert to the more stable 1H tautomer in a process signaled by fluorescence changes that would not be affecting to the d−d spectrum of the complex

Effect of hamstring tightness and fatigue on dynamic stability and agility in physically active young men.

Hamstring extensibility has been defined as a factor to diminished dynamic stability and therefore increased risk of injury. The purpose of this study was to analyse the effects of hamstring tightness and fatigue on dynamic stability and agility. Nineteen participants were divided between the normal extensibility group (NEG) (n = 9, 82.2° ± 12.4°) and hamstrings tightness group (HTG) (n = 10, 64° ± 4.9°) using the passive straight leg raise test. To analyse dynamic stability and agility, they performed the modified Star Excursion Balance Test (mSEBT) and Dynamic Postural Stability Index (DPSI), and hexagon agility test, respectively, before and after a fatigue protocol. A two-way repeated measures ANOVA was used to determine differences among conditions: NEG vs. HTG, and rested vs. fatigued. HTG showed a significantly lower reach in the anterior direction in the mSEBT in pre- and post-fatigue than NEG. Participants in the NEG showed poor stability after landing in the mediolateral direction on DPSI post-fatigue. No significant changes were found in agility related with the group nor fatigue state. Participants with hamstring extensibility reduction has no differences in dynamic stability after landing nor agility after fatigue test, but significantly affects reaching distances during one-leg balance. As a conclusion, a reduction in range of motion in HTG was observed, but no other effects were observed on performance and dynamic stability after a local fatigue protocol depending on hamstring extensibility

Multi-smart and scalable bioligands-free nanomedical platform for intratumorally targeted tambjamine delivery, a difficult to administrate highly cytotoxic drug

Cancer is one of the leading causes of mortality worldwide due, in part, to limited success of some current therapeutic approaches. The clinical potential of many promising drugs is restricted by their systemic toxicity and lack of selectivity towards cancer cells, leading to insufficient drug concentration at the tumor site. To overcome these hurdles, we developed a novel drug delivery system based on polyurea/polyurethane nanocapsules (NCs) showing pH-synchronized amphoteric properties that facilitate their accumulation and selectivity into acidic tissues, such as tumor microenvironment. We have demonstrated that the anticancer drug used in this study, a hydrophobic anionophore named T21, increases its cytotoxic activity in acidic conditions when nanoencapsulated, which correlates with a more efficient cellular internalization. A biodistribution assay performed in mice has shown that the NCs are able to reach the tumor and the observed systemic toxicity of the free drug is significantly reduced in vivo when nanoencapsulated. Additionally, T21 antitumor activity is preserved, accompanied by tumor mass reduction compared to control mice. Altogether, this work shows these NCs as a potential drug delivery system able to reach the tumor microenvironment, reducing the undesired systemic toxic effects. Moreover, these nanosystems are prepared under scalable methodologies and straightforward process, and provide tumor selectivity through a smart mechanism independent of targeting ligands

¿Producen los atentados terroristas selectivos una mayor sintomatología de estrés postraumático en sus víctimas que los atentados indiscriminados?

La literatura científica sobre las consecuencias psicopatológicas derivadas de un atentado terrorista no ha abordado todavía si el tipo de atentado en la dimensión de selectivo frente a indiscriminado puede estar relacionado con el nivel de sintomatología de estrés postraumático que padecen sus víctimas. Puesto que en España las víctimas de atentados terroristas selectivos han sufrido unos niveles muy altos de estrés, amenaza y acoso físico y psicológico y unos niveles muy bajos de apoyo social, tanto antes como después del atentado, cabría esperar que tales víctimas deberían sufrir mayores niveles de sintomatología de estrés postraumático que las víctimas de atentados indiscriminados. Para comprobar esta hipótesis, se aplicó a una muestra de 224 víctimas directas e indirectas (familiares de fallecidos y heridos) del terrorismo en España la Lista de Verificación del Trastorno por Estrés Postraumático, versión específica (PCL-S), y se codificaron los atentados que habían sufrido en cuatro categorías: indiscriminado, selectivo, selectivo e indiscriminado, e indiscriminado con colectivo objetivo. Tras controlar el efecto del sexo, la edad, los años transcurridos desde el atentado y el tipo de víctimas, un análisis de regresión no encontró que el tipo de atentado estuviera relacionado significativamente con el nivel de sintomatología de estrés postraumático. Los resultados se discuten en el contexto de los factores psicosociales que han caracterizado el terrorismo en España y de los factores de vulnerabilidad y protección que predicen los niveles de psicopatología que pueden sufrir las víctimas tras un atentado terrorista

Ligand diversity contributes to the full activation of the jasmonate pathway in Marchantia polymorpha

In plants, jasmonate signaling regulates a wide range of processes from growth and development to defense responses and thermotolerance. Jasmonates, such as jasmonic acid (JA), (+)-7-iso-jasmonoyl-l-isoleucine (JA-Ile), 12-oxo-10,15(Z)-phytodienoic acid (OPDA), and dinor-12-oxo-10,15(Z)-phytodienoic acid (dn-OPDA), are derived from C18 (18 Carbon atoms) and C16 polyunsaturated fatty acids (PUFAs), which are found ubiquitously in the plant kingdom. Bryophytes are also rich in C20 and C22 long-chain polyunsaturated fatty acids (LCPUFAs), which are found only at low levels in some vascular plants but are abundant in organisms of other kingdoms, including animals. The existence of bioactive jasmonates derived from LCPUFAs is currently unknown. Here, we describe the identification of an OPDA-like molecule derived from a C20 fatty acid (FA) in the liverwort Marchantia polymorpha (Mp), which we term (5Z,8Z)-10-(4-oxo-5-((Z)-pent-2-en-1-yl)cyclopent-2-en-1-yl)deca-5,8-dienoic acid (C20-OPDA). This molecule accumulates upon wounding and, when applied exogenously, can activate known Coronatine Insensitive 1 (COI1) -dependent and -independent jasmonate responses. Furthermore, we identify a dn-OPDA-like molecule (Δ4-dn-OPDA) deriving from C20-OPDA and demonstrate it to be a ligand of the jasmonate coreceptor (MpCOI1-Mp Jasmonate-Zinc finger inflorescence meristem domain [MpJAZ]) in Marchantia. By analyzing mutants impaired in the production of LCPUFAs, we elucidate the major biosynthetic pathway of C20-OPDA and Δ4-dn-OPDA. Moreover, using a double mutant compromised in the production of both Δ4-dn-OPDA and dn-OPDA, we demonstrate the additive nature of these molecules in the activation of jasmonate responses. Taken together, our data identify a ligand of MpCOI1 and demonstrate LCPUFAs as a source of bioactive jasmonates that are essential to the immune response of M. polymorpha.Peer reviewe

Specific interaction of citrate with bis(fluorophoric) bibrachial lariat aza-crown in comparison with the other components of the Krebs cycle

Clares Garcia, M. Paz, [email protected] ; Garcia-España Monsonis, Enrique, [email protected] ; Soriano Soto, Concepcion, [email protected] ; Tejero Toquero, Roberto, [email protected]