Discovery of six new class II methanol maser transitions, including the unambiguous detection of three torsionally excited lines toward G 358.931-0.030

We present the unambiguous discovery of six new class II methanol maser transitions, three of which are torsionally excited (vt = 1). The newly discovered 6.18 GHz 17−2 → 18−3 E (vt = 1), 7.68 GHz 124 → 133 A− (vt = 0), 7.83 GHz 124 → 133 A+ (vt = 0), 20.9 GHz 101 → 112 A+ (vt = 1), 44.9 GHz 20 → 31 E (vt = 1), and 45.8 GHz 93 → 102 E (vt = 0) methanol masers were detected toward G 358.931-0.030, where the known 6.68 GHz maser has recently been reported to be undergoing a period flaring. The detection of the vt = 1 torsionally excited lines corroborates one of the missing puzzle pieces in class II maser pumping, but the intensity of the detected emission provides an additional challenge, especially in the case of the very highly excited 6.18 GHz line. Together with the newly detected vt = 0 lines, these observations provide significant new information that can be utilized to improve class II methanol maser modeling. We additionally present detections of 6.68, 19.9, 23.1, and 37.7 GHz class II masers, as well as 36.2 and 44.1 GHz class I methanol masers, and provide upper limits for the 38.3 and 38.5 GHz class II lines. Near simultaneous Australia Telescope Compact Array observations confirm that all 10 of the class II methanol maser detections are co-spatial to ∼0.2 arcsec, which is within the uncertainty of the observations. We find significant levels of linearly polarized emission in the 6.18, 6.67, 7.68, 7.83, 20.9, 37.7, 44.9, and 45.8 GHz transitions, and low levels of circular polarization in the 6.68, 37.7, and 45.8 GHz transitions

The burst mode of accretion in massive star formation with stellar inertia

This is the author accepted manuscript. The final version is available from Oxford University Press via the DOI in this recordData availability: This research made use of the PLUTO code developed at the University of Torino by A. Mignone (http://plutocode.ph.unito.it/). The figures have been produced using the Matplotlib plotting library for the Python programming language (https://matplotlib.org/). The data underlying this article will be shared on reasonable request to the corresponding author.The burst mode of accretion in massive star formation is a scenario linking the initial gravitational collapse of parent pre-stellar cores to the properties of their gravitationally unstable discs and of their accretion-driven bursts. In this study, we present a series of high-resolution 3D radiation-hydrodynamics numerical simulations for young massive stars formed out of collapsing 100 Mo molecular cores spinning with several values of the ratio of rotational-to-gravitational energies beta=5%-9%. The models include the indirect gravitational potential caused by disc asymmetries. We find that this modifies the barycenter of the disc, causing significant excursions of the central star position, which we term stellar wobbling. The stellar wobbling slows down and protracts the development of gravitational instability in the disc, reducing the number and magnitude of the accretion-driven bursts undergone by the young massive stars, whose properties are in good agreement with that of the burst monitored from the massive protostar M17 MIR. Including stellar wobbling is therefore important for accurate modeling disc structures. Synthetic ALMA interferometric images in the millimeter waveband show that the outcomes of efficient gravitational instability such as spiral arms and gaseous clumps can be detected for as long as the disc is old enough and has already entered the burst mode of accretion.Science and Technology Facilities Council (STFC)Ministry of Science and Higher Education of the Russian FederationEuropean Research Council (ERC)MOS

High-mass star formation in the nearby region G352.630-1.067. I. parallax

Young or forming high-mass stars that are nearby and not within a cluster environment have the potential to provide fundamental insights into star formation. In this paper we report such a candidate (G352.630-1.067), for which we have measured the distance through very long baseline interferometry parallax observations of the associated 6.7 GHz class II methanol maser. We determine the distance to the source to be 0.69+0.10-0.08 kpc, which makes it the second nearest high-mass star formation region (HMSFR) known, with only the Orion Nebula being closer. This may place this source, not within a Galactic spiral arm, but in the region between the Local and Sagittarius arms, indicating that molecular clouds in interarm regions may also generate high-mass stars. Kinematic association between this source and the Sagittarius Arm suggests that it may be located in a spur extending outward from this arm. Comparison with the known, nearby HMSFRs (distances less than 1 kpc), reveal that G352.630-1.067 is in a more isolated environment than others, hence providing an excellent candidate for investigations of the processes that form individual high-mass stars. We find a good spatial correlation between the 6.7 GHz methanol maser and high angular resolution images of the infrared outflow, suggesting that the class II methanol masers are closely associated with a jet/outflow in this source

The Ljapunov-Schmidt reduction for some critical problems

This is a survey about the application of the Ljapunov-Schmidt reduction for some critical problems

A third generation vaccine for human visceral leishmaniasis and post kala azar dermal leishmaniasis : First-in-human trial of ChAd63-KH

BACKGROUND: Visceral leishmaniasis (VL or kala azar) is the most serious form of human leishmaniasis, responsible for over 20,000 deaths annually, and post kala azar dermal leishmaniasis (PKDL) is a stigmatizing skin condition that often occurs in patients after successful treatment for VL. Lack of effective or appropriately targeted cell mediated immunity, including CD8+ T cell responses, underlies the progression of VL and progression to PKDL, and can limit the therapeutic efficacy of anti-leishmanial drugs. Hence, in addition to the need for prophylactic vaccines against leishmaniasis, the development of therapeutic vaccines for use alone or in combined immuno-chemotherapy has been identified as an unmet clinical need. Here, we report the first clinical trial of a third-generation leishmaniasis vaccine, developed intentionally to induce Leishmania-specific CD8+ T cells. METHODS: We conducted a first-in-human dose escalation Phase I trial in 20 healthy volunteers to assess the safety, tolerability and immunogenicity of a prime-only adenoviral vaccine for human VL and PKDL. ChAd63-KH is a replication defective simian adenovirus expressing a novel synthetic gene (KH) encoding two Leishmania proteins KMP-11 and HASPB. Uniquely, the latter was engineered to reflect repeat domain polymorphisms and arrangements identified from clinical isolates. We monitored innate immune responses by whole blood RNA-Seq and antigen specific CD8+ T cell responses by IFNγ ELISPOT and intracellular flow cytometry. FINDINGS: ChAd63-KH was safe at intramuscular doses of 1x1010 and 7.5x1010 vp. Whole blood transcriptomic profiling indicated that ChAd63-KH induced innate immune responses characterized by an interferon signature and the presence of activated dendritic cells. Broad and quantitatively robust CD8+ T cell responses were induced by vaccination in 100% (20/20) of vaccinated subjects. CONCLUSION: The results of this study support the further development of ChAd63-KH as a novel third generation vaccine for VL and PKDL. TRIAL REGISTRATION: This clinical trial (LEISH1) was registered at EudraCT (2012-005596-14) and ISRCTN (07766359)

Transcriptomic and metabolomic shifts in rice roots in response to Cr (VI) stress

<p>Abstract</p> <p>Background</p> <p>Widespread use of chromium (Cr) contaminated fields due to careless and inappropriate management practices of effluent discharge, mostly from industries related to metallurgy, electroplating, production of paints and pigments, tanning, and wood preservation elevates its concentration in surface soil and eventually into rice plants and grains. In spite of many previous studies having been conducted on the effects of chromium stress, the precise molecular mechanisms related to both the effects of chromium phytotoxicity, the defense reactions of plants against chromium exposure as well as translocation and accumulation in rice remain poorly understood.</p> <p>Results</p> <p>Detailed analysis of genome-wide transcriptome profiling in rice root is reported here, following Cr-plant interaction. Such studies are important for the identification of genes responsible for tolerance, accumulation and defense response in plants with respect to Cr stress. Rice root metabolome analysis was also carried out to relate differential transcriptome data to biological processes affected by Cr (VI) stress in rice. To check whether the Cr-specific motifs were indeed significantly over represented in the promoter regions of Cr-responsive genes, occurrence of these motifs in whole genome sequence was carried out. In the background of whole genome, the lift value for these 14 and 13 motifs was significantly high in the test dataset. Though no functional role has been assigned to any of the motifs, but all of these are present as promoter motifs in the Database of orthologus promoters.</p> <p>Conclusion</p> <p>These findings clearly suggest that a complex network of regulatory pathways modulates Cr-response of rice. The integrated matrix of both transcriptome and metabolome data after suitable normalization and initial calculations provided us a visual picture of the correlations between components. Predominance of different motifs in the subsets of genes suggests the involvement of motif-specific transcription modulating proteins in Cr stress response of rice.</p

Integration of Evolutionary Features for the Identification of Functionally Important Residues in Major Facilitator Superfamily Transporters

The identification of functionally important residues is an important challenge for understanding the molecular mechanisms of proteins. Membrane protein transporters operate two-state allosteric conformational changes using functionally important cooperative residues that mediate long-range communication from the substrate binding site to the translocation pathway. In this study, we identified functionally important cooperative residues of membrane protein transporters by integrating sequence conservation and co-evolutionary information. A newly derived evolutionary feature, the co-evolutionary coupling number, was introduced to measure the connectivity of co-evolving residue pairs and was integrated with the sequence conservation score. We tested this method on three Major Facilitator Superfamily (MFS) transporters, LacY, GlpT, and EmrD. MFS transporters are an important family of membrane protein transporters, which utilize diverse substrates, catalyze different modes of transport using unique combinations of functional residues, and have enough characterized functional residues to validate the performance of our method. We found that the conserved cores of evolutionarily coupled residues are involved in specific substrate recognition and translocation of MFS transporters. Furthermore, a subset of the residues forms an interaction network connecting functional sites in the protein structure. We also confirmed that our method is effective on other membrane protein transporters. Our results provide insight into the location of functional residues important for the molecular mechanisms of membrane protein transporters