Assessing temporal genetic variation in a cougar population: influence of harvest and neighboring populations

The geography of the Black Hills region of South Dakota and Wyoming may limit connectivity for many species. For species with large energetic demands and large home ranges or species at low densities this can create viability concerns. Carnivores in this region, such as cougars (Puma concolor), have the additive effect of natural and human-induced mortality; this may act to decrease long-term viability. In this study we set out to explore genetic diversity among cougar populations in the Black Hills and surrounding areas. Specifically, our objectives were to first compare genetic variation and effective number of breeders of cougars in the Black Hills during three harvest regimes: pre (2003–2006), moderate (2007–2010), and heavy (2011–2013), to determine if harvest impacted genetic variation. Second, we compared genetic structure of the Black Hills cougar population with cougar populations in neighboring eastern Wyoming and North Dakota. Using 20 microsatellite loci, we conducted genetic analysis on DNA samples from cougars in the Black Hills (n = 675), North Dakota (n = 113), and eastern Wyoming (n = 62) collected from 2001–2013. Here we report that the Black Hills cougar population maintained genetic variation over the three time periods. Our substructure analysis suggests that the maintenance of genetic variation was due to immigration from eastern Wyoming and possibly North Dakota

A cross-sectional cohort of the healthy older adult population’s ability with left right judgment task

Over 100 million US adults suffer from chronic pain, many older adults. In some cases, chronic pain has been shown to alter a person’s body schema and thus their motor imagery ability. Clinically, a measurement of left/right judgment (LRJ) has been used to measure motor imagery. Evidence suggests differences can be found in individuals’ LRJ reaction times and accuracy for those with pain compared to those without. Limited study has been done in the area of LRJ in an older population. The primary objective of this study is to explore LRJ norms for older adults. The secondary aim is to determine if there is a decline in LRJ ability with aging compared to other studies with younger adults. Participants were recruited from a cross-sectional cohort of older adults ranging in age from 55-92 years old. Demographic information of each participant was recorded before testing, including age, sex, handedness, handgrip strength, and a self-report of average cardiovascular and strength training in minutes per week. LRJ testing for hands utilizing the RecogniseTM app was used for recording accuracy and reaction time scores. Twenty-four participants completed the LRJ task (x = 80.25 years old + 8.95). The average accuracy for left and right were 66% and 67%, with reaction time scores of 2.33 seconds and 2.24 seconds, respectively. Reaction time and speed trade-off was found, r = -.483, p = .017. These results show a decline in accuracy and speed compared to younger adults

Exploring the Structures and Substructures of the Andromeda Satellite Dwarf Galaxies Cassiopeia III, Perseus I, and Lacerta I

We present results from wide-field imaging of the resolved stellar populations of the dwarf spheroidal galaxies Cassiopeia III (And XXXII) and Perseus I (And XXXIII), two satellites in the outer stellar halo of the Andromeda galaxy (M31). Our WIYN pODI photometry traces the red giant star population in each galaxy to ~2.5-3 half-light radii from the galaxy center. We use the Tip of the Red Giant Branch (TRGB) method to derive distances of (m-M)_0 = 24.62+/-0.12 mag (839 (+48,-450) kpc, or 156 (+16,-13) kpc from M31) for Cas III and 24.47+/-0.13 mag (738 (+48,-45) kpc, or 351 (+17,-16) kpc from M31) for Per I. These values are consistent within the errors with TRGB distances derived from a deeper Hubble Space Telescope study of the galaxies' inner regions. For each galaxy, we derive structural parameters, total magnitude, and central surface brightness. We also place upper limits on the ratio of neutral hydrogen gas mass to optical luminosity, confirming the gas-poor nature of both galaxies. We combine our data set with corresponding data for the M31 satellite galaxy Lacerta I (And XXXI) from earlier work, and search for substructure within the RGB star populations of Cas III, Per I, and Lac I. We find an overdense region on the west side of Lac I at a significance level of 2.5-3-sigma and a low-significance filament extending in the direction of M31. In Cas III, we identify two modestly significant overdensities near the center of the galaxy and another at two half-light radii. Per I shows no evidence for substructure in its RGB star population, which may reflect this galaxy's isolated nature.Comment: 26 pages, 15 figures, 5 tables. Accepted for publication in The Astronomical Journa

Exploring the Structures and Substructures of the Andromeda Satellite Dwarf Galaxies Cassiopeia III, Perseus I, and Lacerta I

We present results from wide-field imaging of the resolved stellar populations of the dwarf spheroidal galaxies Cassiopeia III (And XXXII) and Perseus I (And XXXIII), two satellites in the outer stellar halo of the Andromeda galaxy (M31). Our WIYN pODI photometry traces the red giant star population in each galaxy to ∼2.5−3 half-light radii from the galaxy center. We use the tip of the red giant branch (TRGB) method to derive distances of (m − M) = 24.62 ± 0.12 mag (839 kpc, or 156 kpc from M31) for Cas III and 24.47 ± 0.13 mag (738 kpc, or 351 kpc from M31) for Per I. These values are consistent within the errors with TRGB distances derived from a deeper Hubble Space Telescope study of the galaxies’ inner regions. For each galaxy, we derive structural parameters, total magnitude, and central surface brightness. We also place upper limits on the ratio of neutral hydrogen gas mass to optical luminosity, confirming the gas-poor nature of both galaxies. We combine our data set with corresponding data for the M31 satellite galaxy Lacerta I (And XXXI) from earlier work and search for substructure within the RGB star populations of Cas III, Per I, and Lac I. We find an overdense region on the west side of Lac I at a significance level of 2.5σ–3σ and a low-significance filament extending in the direction of M31. In Cas III, we identify two modestly significant overdensities near the center of the galaxy and another at two half-light radii. Per I shows no evidence for substructure in its RGB star population, which may reflect this galaxy’s isolated nature. © 2023 Institute of Physics Publishing. All rights reservedThe authors wish to thank the anonymous referee for providing valuable comments and suggestions for improvements to the manuscript. We are grateful to the staff of the WIYN 3.5 m Observatory and Kitt Peak National Observatory for their help during our WIYN pODI observing runs. We also thank the staff members at WIYN, NOIRLab, and Indiana University Pervasive Technology Institute for designing and implementing the ODI Pipeline, Portal, and Archive (ODI-PPA) and assisting us with the pODI data reduction. We made use of the odi-tools python package, written by William Janesh and Owen Boberg, to produce final stacked science-ready WIYN pODI images for this study. K.L.R. and N.J.S. were supported by NSF Astronomy & Astrophysics Research grant No. AST-1615483 during part of the period when this research was carried out. Research by D.C. is supported by NSF grant AST-1814208. K.S. acknowledges support from the Natural Sciences and Engineering Research Council of Canada (NSERC). We are grateful to the Indiana University (IU) College of Arts and Sciences for funding IU's share of the WIYN 3.5 m telescope. This research has made use of the NASA/IPAC Extragalactic Database (NED), which is funded by the National Aeronautics and Space Administration and operated by the California Institute of Technology

Constraining the reflective properties of WASP-178 b using CHEOPS photometry

Context. Multiwavelength photometry of the secondary eclipses of extrasolar planets is able to disentangle the reflected and thermally emitted light radiated from the planetary dayside. Based on this, we can measure the planetary geometric albedo Ag, which is an indicator of the presence of clouds in the atmosphere, and the recirculation efficiency ϵ, which quantifies the energy transport within the atmosphere. Aims. We measure Ag and ϵ for the planet WASP-178 b, a highly irradiated giant planet with an estimated equilibrium temperature of 2450 K. Methods. We analyzed archival spectra and the light curves collected by CHEOPS and TESS to characterize the host WASP-178, refine the ephemeris of the system, and measure the eclipse depth in the passbands of the two telescopes. Results. We measured a marginally significant eclipse depth of 70 ± 40 ppm in the TESS passband, and a statistically significant depth of 70 ± 20 ppm in the CHEOPS passband. Conclusions. Combining the eclipse-depth measurement in the CHEOPS (λeff = 6300 Å) and TESS (λeff = 8000 Å) passbands, we constrained the dayside brightness temperature of WASP-178 b in the 2250–2800 K interval. The geometric albedo 0.10.7 makes WASP-178 b an interesting laboratory for testing the current heat-recirculation models

Rnd3 as a Novel Target to Ameliorate Microvascular Leakage

Background -Microvascular leakage of plasma proteins is a hallmark of inflammation that leads to tissue dysfunction. There are no current therapeutic strategies to reduce microvascular permeability. The purpose of this study was to identify the role of Rnd3, an atypical Rho family GTPase, in the control of endothelial barrier integrity. The potential therapeutic benefit of Rnd3 protein delivery to ameliorate microvascular leakage was also investigated. Methods and Results-Using immunofluorescence microscopy, Rnd3 was observed primarily in cytoplasmic areas around the nuclei of human umbilical vein endothelial cells. Permeability to fluorescein isothiocyanate-albumin and transendothelial electrical resistance of human umbilical vein endothelial cell monolayers served as indices of barrier function, and RhoA, Rac1, and Cdc42 activities were determined using G-LISA assays. Overexpression of Rnd3 significantly reduced the magnitude of thrombin-induced barrier dysfunction, and abolished thrombin-induced Racl inactivation. Depleting Rnd3 expression with siRNA significantly extended the time course of thrombin-induced barrier dysfunction and Racl inactivation. Time-lapse microscopy of human umbilical vein endothelial cells expressing GFP-actin showed that co-expression of mCherry-Rnd3 attenuated thrombin-induced reductions in local lamellipodia that accompany endothelial barrier dysfunction. Lastly, a novel Rnd3 protein delivery method reduced microvascular leakage in a rat model of hemorrhagic shock and resuscitation, assessed by both intravital microscopic observation of extravasation of fluorescein isothiocyanate-albumin from the mesenteric microcirculation, and direct determination of solute permeability in intact isolated venules. Conclusions-The data suggest that Rnd3 can shift the balance of RhoA and Racl signaling in endothelial cells. In addition, our findings suggest the therapeutic, anti-inflammatory potential of delivering Rnd3 to promote endothelial barrier recovery during inflammatory challenge

Mechanical Dispersion Assessed by Myocardial Strain in Patients After Myocardial Infarction for Risk Prediction of Ventricular Arrhythmia

ObjectivesThe aim of this study was to investigate whether myocardial strain echocardiography can predict ventricular arrhythmias in patients after myocardial infarction (MI).BackgroundLeft ventricular (LV) ejection fraction (EF) is insufficient for selecting patients for implantable cardioverter-defibrillator (ICD) therapy after MI. Electrical dispersion in infarcted myocardium facilitates malignant arrhythmia. Myocardial strain by echocardiography can quantify detailed regional and global myocardial function and timing. We hypothesized that electrical abnormalities in patients after MI will lead to LV mechanical dispersion, which can be measured as regional heterogeneity of contraction by myocardial strain.MethodsWe prospectively included 85 post-MI patients, 44 meeting primary and 41 meeting secondary ICD prevention criteria. After 2.3 years (range 0.6 to 5.5 years) of follow-up, 47 patients had no and 38 patients had 1 or more recorded arrhythmias requiring appropriate ICD therapy. Longitudinal strain was measured by speckle tracking echocardiography. The SD of time to maximum myocardial shortening in a 16-segment LV model was calculated as a parameter of mechanical dispersion. Global strain was calculated as average strain in a 16-segment LV model.ResultsThe EF did not differ between ICD patients with and without arrhythmias occurring during follow-up (34 ± 11% vs. 35 ± 9%, p = 0.70). Mechanical dispersion was greater in ICD patients with recorded ventricular arrhythmias compared with those without (85 ± 29 ms vs. 56 ± 13 ms, p < 0.001). By Cox regression, mechanical dispersion was a strong and independent predictor of arrhythmias requiring ICD therapy (hazard ratio: 1.25 per 10-ms increase, 95% confidence interval: 1.1 to 1.4, p < 0.001). In patients with an EF >35%, global strain showed better LV function in those without recorded arrhythmias (−14.0% ± 4.0% vs. −12.0 ± 3.0%, p = 0.05), whereas the EF did not differ (44 ± 8% vs. 41 ± 5%, p = 0.23).ConclusionsMechanical dispersion was more pronounced in post-MI patients with recurrent arrhythmias. Global strain was a marker of arrhythmias in post-MI patients with relatively preserved ventricular function. These novel parameters assessed by myocardial strain may add important information about susceptibility for ventricular arrhythmias after MI

The Association Between Self-Reported Symptoms of Recent Airway Infection and CRP Values in a General Population: The Tromsø Study: Tromsø 6

C-reactive protein (CRP) is a much used biomarker for respiratory tract infection; however, the influence of airway infection on the CRP level in the general population has not been well described. The study aimed to evaluate the impact of recent symptoms of airway infection on the CRP level and how the predictive power of other known CRP predictors is influenced by taking respiratory symptoms into account. A total of 6,325 participants, aged 38–87 years, in the Tromsø Study, a repeated population-based survey, were examined with questionnaires, measurements of height and weight, spirometry, and high-sensitivity CRP analyses. The mean CRP value was 2.86 mg/L, and the geometric mean was 1.51 mg/L. Geometric means above 2.0 mg/L were found in the subgroups with the following characteristics: self-reported COPD, diabetes, recent symptoms of airway infection, forced expiratory volume in 1 s (FEV1) <80% predicted, body mass index (BMI) ≥30, and subjects treated with inhaled or oral corticosteroids. Among the subjects who reported recent airway infection, 10.5% had a CRP value of ≥10 mg/L, compared to 3.3% among the remaining participants. By multivariate analysis, BMI was the strongest independent predictor of the CRP level, followed by recent airway infection, FEV1% predicted, age, and current smoking. The study clearly demonstrates that a report of recent symptoms of airway infection strongly predicts the CRP level in the population. Such symptoms were shared rather equally between subgroups with increased CRP level, and the risk of being an important confounder in epidemiological studies is probably low. In the clinical setting, care should be taken when using the CRP level as a guide for medical prevention of chronic diseases

Indications for Absorbable Steroid-Eluting Sinus Implants: Viewpoint via the Delphi Method

Absorbable steroid-eluting sinus implants provide targeted corticosteroid release over a sustained period and are designed to prevent both undesirable adhesion formation and sinus ostia restenosis. Here, we highlight the key evidence of these implants to date and query a group of experts via a Delphi process on the indications and optimal timing for intraoperative or in-office placement of these implants. Six of a total of 12 statements reached consensus and were accepted. Overall, experts largely agree that intraoperative or in-office use of steroid-eluting stents could be considered for patients: (1) who are diabetic or intolerant of oral steroids, (2) undergoing extended frontal sinus surgery, and (3) with recurrent stenosis. Given the lack of expert consensus on other key statements, clinicians should carefully consider these treatment options on a case-by-case basis after shared decision-making