Conversion possibilities of piston twin engine Beechcraft B60 Duke aircraft into prop-jet aircraft

Diplomová práce se zabývá problematikou konverze dvoumotorového pístového letounu Beechcraft B60 Duke na turbovrtulové motory Walter M601 nebo Pratt & Whitney Canada PT6. Práce se zaměřuje na finanční analýzu vlastního provedení přestavby, potřebných prostředků pro certifikaci, schválení do provozu v České republice a stanovení návratnosti investice. Dále se zabývá vypracováním návrhů letových a výkonových charakteristik, provozních nákladů pro oba navrhované motory a porovnáním se současnými charakteristikami a provozními náklady.Master’s thesis deals with the issue of a conversion of a twin-engined piston aircraft Beechcraft B60 Duke into turboprop engines Walter M601 or Pratt & Whitney Canada PT6.The paper focuses on a financial analysis of conducting of the conversion, resources needed for a certification, authorization for the traffic in the Czech Republic and determination of the rate of return on investment.The thesis further deals with elaborating of the flight and prerformance characteristics, operation costs of both of the proposed engines and compares them with current characteristics and operation costs.

Mediator-less immunodetection with voltage-controlled intrinsic amplification for ultrasensitive and rapid detection of microorganism pathogens

A mediator-less immunodetection method for microorganisms is realized by incorporating the newly developed field-effect enzymatic detection (FEED) technique with the conventional electrochemical immunosensing approach. The gating voltage of FEED facilitates the transduction of electrical signal through the bulky immune complex so that the detection does not rely on the use of mediators or other diffusional substances. The voltage-controlled intrinsic amplification provided by the detection system allows detection in low-concentration samples without target pre-enrichment, leading to ultrasensitive and rapid detection. The detection approach is demonstrated with E. coliO157:H7, a model microorganism, in milk with an estimated detection limit of 20 CFU mL−1 (where CFU is a colony-forming unit) without performing sample pre-enrichment and centrifugation of sample followed by the resuspension of the pellet in a buffer solution, resulting in a significantly shortened assay time of 67 min. Optimizing the gating voltage resulted in the detection of 12 CFU mL−1 of the bacterium in milk. The novel detection approach can be used as a detection platform for ultrasensitive, specific and rapid detection of microorganism pathogens

Terminology of bioanalytical methods (IUPAC Recommendations 2018)

Recommendations are given concerning the terminology of methods of bioanalytical chemistry. With respect to dynamic development particularly in the analysis and investigation of biomacromolecules, terms related to bioanalytical samples, enzymatic methods, immunoanalytical methods, methods used in genomics and nucleic acid analysis, proteomics, metabolomics, glycomics, lipidomics, and biomolecules interaction studies are introduced

Terminology of bioanalytical methods (IUPAC Recommendations 2018)

free accessRecommendations are given concerning the terminology of methods of bioanalytical chemistry. With respect to dynamic development particularly in the analysis and investigation of biomacromolecules, terms related to bioanalytical samples, enzymatic methods, immunoanalytical methods, methods used in genomics and nucleic acid analysis, proteomics, metabolomics, glycomics, lipidomics, and biomolecules interaction studies are introduced.Peer reviewe

Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL(-1) in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals

Effect of Particle Size and Surface Chemistry of Photon Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL−1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals