High correlation between Chagas' disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in bolivian children living in an endemic area

Submitted by sandra infurna ([email protected]) on 2016-06-29T15:31:55Z No. of bitstreams: 1 carlos20_morel_etal_IOC_1994.pdf: 578671 bytes, checksum: 49c1bfe1a3334487aede673ca532654a (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-06-29T15:43:03Z (GMT) No. of bitstreams: 1 carlos20_morel_etal_IOC_1994.pdf: 578671 bytes, checksum: 49c1bfe1a3334487aede673ca532654a (MD5)Made available in DSpace on 2016-06-29T15:43:03Z (GMT). No. of bitstreams: 1 carlos20_morel_etal_IOC_1994.pdf: 578671 bytes, checksum: 49c1bfe1a3334487aede673ca532654a (MD5) Previous issue date: 1994Submitted by Angelo Silva ([email protected]) on 2016-07-07T11:16:53Z No. of bitstreams: 3 carlos20_morel_etal_IOC_1994.pdf.txt: 19093 bytes, checksum: d5ec7ae508e9468c358833ecf7cf1076 (MD5) carlos20_morel_etal_IOC_1994.pdf: 578671 bytes, checksum: 49c1bfe1a3334487aede673ca532654a (MD5) license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-07-07T12:13:56Z (GMT) No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) carlos20_morel_etal_IOC_1994.pdf: 578671 bytes, checksum: 49c1bfe1a3334487aede673ca532654a (MD5) carlos20_morel_etal_IOC_1994.pdf.txt: 19093 bytes, checksum: d5ec7ae508e9468c358833ecf7cf1076 (MD5)Made available in DSpace on 2016-07-07T12:13:56Z (GMT). No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) carlos20_morel_etal_IOC_1994.pdf: 578671 bytes, checksum: 49c1bfe1a3334487aede673ca532654a (MD5) carlos20_morel_etal_IOC_1994.pdf.txt: 19093 bytes, checksum: d5ec7ae508e9468c358833ecf7cf1076 (MD5) Previous issue date: 1994Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.UMR CNRS/ ORSTOM, Génétique Moléculaire des Parasites et des Vecteurs. CP 9214, La Paz, Bolivia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Universidad Mayor de San Andres. Instituto Boliviano de Biologia de Altura. La Paz, Bolivia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.UMR CNRS/ ORSTOM, Génétique Moléculaire des Parasites et des Vecteurs. CP 9214, La Paz, Bolivia.The detection of Tr)tpunosomu crirzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas’ disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological testing, buffy coat analysis and PCR diagnosis. 26 of the 28 serology-positive individuals were also positive by PCR. In addition, two serology-negative children gave a positive result by PCR, including one who was positive in the buffy coat test. These results suggest that PCR detection of T. cruzi DNA in blood can be a very useful complement to serology in Chagas’ disease diagnosis in Bolivia

Curcumin-loaded polymeric and lipid nanocapsules: preparation, characterization and chemical stability evaluation

Polymeric and lipid nanocapsules suspensions of the natural compound curcumin were prepared in order to overcome limitations associated with its clinical applications, such as poor aqueous solubility and susceptibility to hydrolytic and photochemical degradation. Nanocapsule suspensions were prepared by nanoprecipitation and phase inversion methods, respectively. The curcumin formulations were investigated for physicochemical characteristics and in vitro drug release. The hydrolytic and photochemical degradation of the drug associated with the nanocarriers was also determined. For all formulations, the entrapment efficiency values were higher than 99 %. The aqueous colloidal suspensions of curcumin resulted in an increase in drug concentration by a factor of up to 46.103 times. Moreover, stability studies indicated that nanoencapsulation slows down the hydrolytic and photochemical degradations of curcumin. The strategy of nanoencapsulation into polymeric and lipid nanocapsules produced a formulation of curcumin with high drug loading and improved stability, representing a good strategy for the delivery of this drug.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Curcumin-loaded polymeric and lipid nanocapsules: preparation, characterization and chemical stability evaluation

Polymeric and lipid nanocapsules suspensions of the natural compound curcumin were prepared in order to overcome limitations associated with its clinical applications, such as poor aqueous solubility and susceptibility to hydrolytic and photochemical degradation. Nanocapsule suspensions were prepared by nanoprecipitation and phase inversion methods, respectively. The curcumin formulations were investigated for physicochemical characteristics and in vitro drug release. The hydrolytic and photochemical degradation of the drug associated with the nanocarriers was also determined. For all formulations, the entrapment efficiency values were higher than 99 %. The aqueous colloidal suspensions of curcumin resulted in an increase in drug concentration by a factor of up to 46.103 times. Moreover, stability studies indicated that nanoencapsulation slows down the hydrolytic and photochemical degradations of curcumin. The strategy of nanoencapsulation into polymeric and lipid nanocapsules produced a formulation of curcumin with high drug loading and improved stability, representing a good strategy for the delivery of this drug.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Application of cupuassu shell as biosorbent for the removal of textile dyes from aqueous solution

AbstractThe cupuassu shell (Theobroma grandiflorum) which is a food residue was used in its natural form as biosorbent for the removal of C.I. Reactive Red 194 and C.I. Direct Blue 53 dyes from aqueous solutions. This biosorbent was characterized by infrared spectroscopy, scanning electron microscopy, and nitrogen adsorption/desorption curves. The effects of pH, biosorbent dosage and shaking time on biosorption capacities were studied. In acidic pH region (pH 2.0) the biosorption of the dyes were favorable. The contact time required to obtain the equilibrium was 8 and 18 h at 298 K, for Reactive Red 194 and Direct Blue 53, respectively. The Avrami fractionary-order kinetic model provided the best fit to experimental data compared with pseudo-first-order, pseudo-second-order and chemisorption kinetic adsorption models. The equilibrium data were fitted to Langmuir, Freundlich, Sips and Radke–Prausnitz isotherm models. For both dyes the equilibrium data were best fitted to the Sips isotherm model

Monte Carlo Simulation of Bony Heterogeneity Effects on Dose Profile for Small Irradiation Field in Radiotherapy

In the radiotherapy treatment planning of a lesion located in the head region with small field radiation beams, the heterogeneity corrections play an important role. In this work, we investigated the influence of a bony heterogeneity on dose profile inside a soft tissue phantom containing a bony material. PDD curves were obtained by simulation using the Monte Carlo code EGSnrc and employing Eclipse® treatment planning system algorithms (Batho, Modified Batho, Equivalent TAR and Anisotropic Analytic Algorithm) for a 15 MV photon beam and field sizes of 2×2 and 10×10 cm2. The Equivalent TAR method exhibited better agreement with Monte Carlo simulations for the 2×2 cm2 field size. The magnitude of the effect on PDD due to the bony heterogeneity for 1×1, 2×2 and 10×10 cm2 field sizes increases to 10, 5 and 3%, respectively

Quasi-normal modes of the scalar hairy black hole

We calculate QNMs of the scalar hairy black hole in the AdS background using Horowitz-Hubeny method for the potential that is not known in analytical form. For some black hole parameters we found pure imaginary frequencies. Increasing of the scalar field mass does not cause the imaginary part to vanish, it reaches some minimum and then increases, thus in the case under consideration the infinitely long living modes (quasi-resonances) do not appear.Comment: 17 pages, 17 figures, LaTe

Methodology for a Comprehensive Health Impact Assessment in Water Supply and Sanitation Programmes for Brazil

Based on the broader concept of health proposed by the Pan-American Health Organization/World Health Organization (PAHO/ WHO), 2018, and the absence in the literature of indices that translate the causal relationship between sanitation and health, a methodology for assessing the health impact of a water and sanitation programmes, known as a Health Impact Assessment (HIA), was developed, specifically in the Brazilian context, and focused on a school in the northeast of the country. Through exploratory and descriptive evidence, and using documentary research as a method, a retrospective survey was carried out from 2000 to 2022 using documents proposing evaluation methodologies. A single document was found to fit the research objective, which was used to develop the proposed HIA methodology. Development of the methodology consisted of two stages: definition of the health dimensions and selection of the indicators making up each dimension. The HIA methodology was then applied to a school in northeast Brazil to test its use, before a water-efficient management intervention was going to be used. The overall score of 46% indicated that there was room for improvement, which the new management approach could facilitate. This methodology is therefore proposed to be an instrument for the evaluation of public water and sanitation policies, thus assisting managers in the decision-making process and in guiding sanitation programs and plans

Curcumin-loaded polymeric and lipid nanocapsules: preparation, characterization and chemical stability evaluation

Polymeric and lipid nanocapsules suspensions of the natural compound curcumin were prepared in order to overcome limitations associated with its clinical applications, such as poor aqueous solubility and susceptibility to hydrolytic and photochemical degradation. Nanocapsule suspensions were prepared by nanoprecipitation and phase inversion methods, respectively. The curcumin formulations were investigated for physicochemical characteristics and in vitro drug release. The hydrolytic and photochemical degradation of the drug associated with the nanocarriers was also determined. For all formulations, the entrapment efficiency values were higher than 99 %. The aqueous colloidal suspensions of curcumin resulted in an increase in drug concentration by a factor of up to 46.103 times. Moreover, stability studies indicated that nanoencapsulation slows down the hydrolytic and photochemical degradations of curcumin. The strategy of nanoencapsulation into polymeric and lipid nanocapsules produced a formulation of curcumin with high drug loading and improved stability, representing a good strategy for the delivery of this drug.Colegio de Farmacéuticos de la Provincia de Buenos Aire

New Multicomponent Forms of the Antiretroviral Nevirapine with Improved Dissolution Performance

In the pharmaceutical area, some drugs exhibit physicochemical properties that adversely affect the formulation processes for bioavailability and effectiveness. Nevirapine (NVP) is an antiretroviral drug that presents low aqueous solubility, which directly impacts its bioavailability. Among all possible modifications, multicomponent crystals, such as cocrystals and eutectic compositions, have been successfully used to improve the solubility of drugs. In this work, the propensity of the formation of multicomponent systems of NVP with seven possible coformers were predicted and tested: salicylic acid (SA), 3-hydroxybenzoic acid (3HBZC), 4-hydroxybenzoic acid (4HBZC), saccharin (SAC), theophylline (THEO), caffeine (CAF), and urea (URE). Results indicate that NVP-SA, NVP-SAC, NVP-3HBZC, and NVP-4HBZC are cocrystals, whereas NVP-THEO and NVP-CAF are eutectic materials, and NVP-URE is a solid physical mixture. A temperature-dependent disorder behavior was identified for NVP-SA cocrystal. Dissolution studies for the eutectic materials are reported, evidencing that these materials exhibit a significant increase in NVP dissolution kinetics.Fil: Costa, Rogeria Nunes. Universidade Federal de Sao Paulo; BrasilFil: Reviglio, Ana Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Siedler, Sana. Universidade Federal de Santa Catarina; BrasilFil: Cardoso, Simone G.. Universidade Federal de Santa Catarina; BrasilFil: Garro Linck, Yamila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Monti, Gustavo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Carvalho, Alexandre Magnus G.. Centro Nacional de Pesquisa em Energia e Materiais; BrasilFil: Resende, Jackson A. L. C.. Universidade Federal do Mato Grosso do Sul; BrasilFil: Chaves, Marcelo H. C.. Fundación Oswaldo Cruz; BrasilFil: Rocha, Helvético Vinícius Antunes. Fundación Oswaldo Cruz; BrasilFil: Choquesillo Lazarte, Duane. Consejo Superior de Investigaciones Científicas; España. Universidad de Granada; EspañaFil: Infantes, Lourdes. Consejo Superior de Investigaciones Científicas; España. Instituto de Química Física Rocasolano; EspañaFil: Cuffini, Silvia Lucia. Universidade de Sao Paulo; Brasi

Use of Arthropod Rarity for Area Prioritisation: Insights from the Azorean Islands

We investigated the conservation concern of Azorean forest fragments and the entire Terceira Island surface using arthropod species vulnerability as defined by the Kattan index, which is based on species rarity. Species rarity was evaluated according to geographical distribution (endemic vs. non endemic species), habitat specialization (distribution across biotopes) and population size (individuals collected in standardized samples). Geographical rarity was considered at ‘global’ scale (species endemic to the Azorean islands) and ‘regional’ scale (single island endemics)