Direct and indirect effects of climatic variations on the interannual variability in net ecosystem exchange across terrestrial ecosystems

Climatic variables not only directly affect the interannual variability (IAV) in net ecosystem exchange of CO2 (NEE) but also indirectly drive it by changing the physiological parameters. Identifying these direct and indirect paths can reveal the underlying mechanisms of carbon (C) dynamics. In this study, we applied a path analysis using flux data from 65 sites to quantify the direct and indirect climatic effects on IAV in NEE and to evaluate the potential relationships among the climatic variables and physiological parameters that represent physiology and phenology of ecosystems. We found that the maximum photosynthetic rate was the most important factor for the IAV in gross primary productivity (GPP), which was mainly induced by the variation in vapour pressure deficit. For ecosystem respiration (RE), the most important drivers were GPP and the reference respiratory rate. The biome type regulated the direct and indirect paths, with distinctive differences between forests and non-forests, evergreen needleleaf forests and deciduous broadleaf forests, and between grasslands and croplands. Different paths were also found among wet, moist and dry ecosystems. However, the climatic variables can only partly explain the IAV in physiological parameters, suggesting that the latter may also result from other biotic and disturbance factors. In addition, the climatic variables related to NEE were not necessarily the same as those related to GPP and RE, indicating the emerging difficulty encountered when studying the IAV in NEE. Overall, our results highlight the contribution of certain physiological parameters to the IAV in C fluxes and the importance of biome type and multi-year water conditions, which should receive more attention in future experimental and modelling research

Effects of Mowing on Methane Uptake in a Semiarid Grassland in Northern China

Background: Mowing is a widely adopted management practice for the semiarid steppe in China and affects CH4 exchange. However, the magnitude and the underlying mechanisms for CH 4 uptake in response to mowing remain uncertain. Methodology/Principal Findings: In two consecutive growing seasons, we measured the effect of mowing on CH 4 uptake in a steppe community. Vegetation was mowed to 2 cm (M2), 5 cm (M5), 10 cm (M10), 15 cm (M15) above soil surface, respectively, and control was set as non-mowing (NM). Compared with control, CH4 uptake was substantially enhanced at almost all the mowing treatments except for M15 plots of 2009. CH4 uptake was significantly correlated with soil microbial biomass carbon, microbial biomass nitrogen, and soil moisture. Mowing affects CH 4 uptake primarily through its effect on some biotic factors, such as net primary productivity, soil microbial C\N supply and soil microbial activities, while soil temperature and moisture were less important. Conclusions/Significance: This study found that mowing affects the fluxes of CH4 in the semiarid temperate steppe of north China

Age effect of deafening on stereotyped song maintenance in adult male bengalese finches Lonchura striata domestica

Birdsong is a complex learned vocal behavior that relies on auditory experience for development. However, it appears that among different species of close-ended songbirds, there are some variations in the necessity of auditory feedback for maintaining stereotyped adult song. In zebra finches, the deterioration of adult songs following deafness depends on the birds’ age. It is unknown whether this age effect is a general rule in other avian species as well. Therefore, we chose Bengalese finches, whose songs show more complexity and have much heavier dependency on auditory feedback than that of zebra finches, to compare the degree of song degradation after hearing loss in old (over 18 months old) and young adult birds (5–6 months old). We found that both syllable sequence and syllable phonology were much less severely affected by deafening in old adults than that in young ones. Moreover, young adults almost lost their capability to sing trills over 6 months following deafening, while old birds continued to sing plenty of trills and trilled syllables after the same period of deafening. Our results suggest that age plays an important role in affecting the dependency of adult song maintenance on auditory feedback in Bengalese finches. Furthermore, the age dependency may be a general phenomenon in different species of close-ended songbirds [Current Zoology 55(3):212–218, 2009]

Astragalin protects the liver from oxidative damage by modulating the lnc XIST/miR-155-5p/Nrf2 axis

Astragalin (AG), a natural flavonoid present in white mulberry and plants belonging to Rosaceae and other families, has various biological functions. However, the potential mechanism through which it protects the liver from injury caused by oxidative stress has not yet been reported. In this study, we aimed to elucidate the mechanism underlying the hepatoprotective effect of AG. We found that AG treatment inhibited the oxidative stress caused by H2O2 and promoted Nrf2 nuclear translocation in an LO2 cell model. Furthermore, the expression of Nrf2 and its downstream antioxidant genes was increased. In the C57BL/6J mouse model, we found AG treatment prevented alcohol-induced acute liver injury in mice by reducing lipid peroxidation and enhancing antioxidant activity. Consistent with in vitro results, we observed elevated expression of Nrf2 and its downstream antioxidant genes in mouse liver tissues. To further explore the underlying mechanisms, bioinformatics analysis was used to detect miRNAs and lnc RNAs that regulate the expression of Nrf2. Our findings suggest that AG may protect the liver from damage caused by oxidative stress by modulating the lnc XIST/miR-155-5p/Nrf2 axis

Inversion of Rice Biophysical Parameters Using Simulated Compact Polarimetric SAR C-Band Data

Timely and accurate estimation of rice parameters plays a significant role in rice monitoring and yield forecasting for ensuring food security. Compact-polarimetric (CP) synthetic aperture radar (SAR), a good compromise between the dual- and quad-polarized SARs, is an important part of the new generation of Earth observation systems. In this paper, the ability of CP SAR data to retrieve rice biophysical parameters was explored using a modified water cloud model. The results showed that S1 was superior to other CP variables in rice height inversion with a coefficient of determination (R2) of 0.92 and a root-mean-square error (RMSE) of 5.81 cm. RL was the most suitable for inverting the volumetric water content of the rice canopy, with an R2 of 0.95 and a RMSE of 0.31 kg/m3. The m-χ decomposition produced the highest accuracies for the ear biomass: R2 was 0.89 and RMSE was 0.17 kg/m2. The highest accuracy of leaf area index (LAI) retrieval was obtained for RH (right circular transmit and horizontal linear receive) with an R2 of 0.79 and a RMSE of 0.33. This study illustrated the capability of CP SAR data with respect to retrieval of rice biophysical parameters, especially for height, volumetric water content of the rice canopy, and ear biomass, and this mode may offer the best option for rice-monitoring applications because of swath coverage

PU.1 interacts with KLF7 to suppress differentiation and promote proliferation in chicken preadipocytes

Krüppel-like factor 7 (KLF7) is a negative regulator of preadipocyte differentiation. Our previous KLF7 ChIP-seq analysis showed that the binding motif of PU.1 was found among the KLF7 binding peaks, indicating that an interaction between KLF7 and PU.1 at preadipocyte gene promoters and other regulatory elements might be common. Here, Co-IP and FRET assays are used to confirm that PU.1 can directly bind to KLF7 and enhance the transcription activity of cyclin-dependent kinase inhibitor 3 ( CDKN3), which is a downstream target gene of KLF7. We show that the PU.1 expression level is decreased during preadipocyte differentiation. Furthermore, PU.1 overexpression and knockdown experiments reveal that PU.1 negatively regulates chicken preadipocyte differentiation, as evidenced by appropriate changes in lipid droplet accumulation and altered expressions of PPARγ, FAS, and PLIN. In addition, PU.1 overexpression promotes preadipocyte proliferation, while knockdown of PU. 1 inhibits preadipocyte proliferation. We further demonstrate that PU.1 inhibits differentiation and promotes proliferation in preadipocytes, in part by directly interacting with KLF7

Supplementary data for the mechanism for cleavage of three typical glucosidic bonds induced by hydroxyl free radical

The data presented in this article are related to the research article entitled âThe mechanism for cleavage of three typical glucosidic bonds induced by hydroxyl free radicalâ (Dai et al., 2017) [1]. This article includes the structures of three kinds of disaccharides such as maltose, fructose and cellobiose, the diagrammatic sketch of the hydrogen abstraction reaction of the disaccharides by hydroxyl radical, the structure of the transition states for pyran ring opening of moiety A and cleavage of α(1â2) glycosidic bond starting from the hydrogen abstraction of C6âH in moiety A of sucrose, the transition state structure for cleavage of α(1â2) glycosidic bond starting from the hydrogen abstraction of C1â²-H in moiety B of sucrose, the transition state structure, sketch for the reaction process and relative energy change of the reaction pathway for direct cleavage of α(1â4) glycosidic bond starting from hydrogen abstraction of C6â²âH of moiety B of maltose