Bugeac Artists in Unity and Variety of Their Creative Manners

The article deals with the creative activities of the representatives of Ismail Municipal Organization of the National Union of Ukrainian Artists (IMO NUUA). It provides the information about the artists and craftsmen, who are the members of the above mentioned professional creative association, existing since 2008. Data is presented as of 2019.&nbsp

Дослідження процесу попереднього обмолоту зерна робочими органами жниварки комбайна

We improved the combined technological process of transportation and threshing of GSM and developed the device of preliminary grain threshing for a grain header of KZS 9-1 «Stavutich» harvester, which made it possible to separate 30–35 % of grain at the early phases of its transportation to TSS of a combine. We established that the coefficient of separation of grain from cones in a header with an intermediate thrashing drum, which contains four additional tooth-shaped bars of 30 mm in height, has the highest (0.32) value.We developed a calculation and experimental method for determining the amount (degree) of grain separation by a device of a header of a combine harvester. The method is based on the results of simulation of the combined process of transportation and threshing of grain caused by the interaction of a drum with grain and straw mass.We analyzed and proved the complex influence of structural parameters of the device and modes of implementation of the combined process of transportation and threshing of GSM at the level of separation of grain. And this make it possible to establish a theoretical dependence of the grain separation coefficient. The noted theoretical dependence provides the possibility to substantiate rational parameters and operating modes of the device of preliminary grain threshing of a combine harvester analytically.We determined dependences of the grain separation coefficient on the speed of a combine experimentally. The dependences take into account cancellations of mechanized technological operations caused by changes in kinematic operating modes of a combine, a number of stops and their height on a drum of a device of preliminary grain threshingИсследован технологический процесс обмолота зерновых культур устройством предварительного обмолота зерна жатки зерноуборочного комбайна. Разработан экспериментально-расчетный метод оценки уровня отделения зерна устройством. Получена теоретическая зависимость коэффициента отделения зерна, которая устанавливает системную взаимосвязь между параметрами и режимами работы устройства жатки и зерноуборочного комбайна. Экспериментально установлены зависимости коэффициента отделения зерна в зависимости от скорости движения комбайнаДосліджено технологічний процес обмолоту зернових культур пристроєм попереднього обмолоту зерна жниварки зернозбирального комбайна. Розроблено експериментально-розрахунковий метод оцінювання рівня відділення зерна пристроєм. Отримано теоретичну залежність коефіцієнта відділення зерна, яка встановлює системний взаємозв'язок між параметрами та режимами функціонування пристрою жниварки та зернозбирального комбайна. Експериментально встановлено залежності коефіцієнта відділення зерна від швидкості руху комбайн

METHODOLOGICAL AND TECHNOLOGICAL ASPECTS OF THE FUTURE TEACHER'S COMPETENCE FORMATION PROCESS MANAGEMENT

In the presentation materials of the article, being the result of many years of scientific studies of authors, the ideology of efficient and effective management of the future teacher's formation is reflected

OBJECTIVE CONTROL IN CONDITIONS OF PERSONALLY FOCUSED TRAINING PROCESS

On the example of tracking the mastering procedure (cognitive task) peculiarities the possibility to implement the technological scheme of objective control in education as the most important mechanism ensuring the efficiency of this process is revealed in the article through such parameter as awareness

Scanning ion conductance microscopy: a convergent high-resolution technology for multi-parametric analysis of living cardiovascular cells

Cardiovascular diseases are complex pathologies that include alterations of various cell functions at the levels of intact tissue, single cells and subcellular signalling compartments. Conventional techniques to study these processes are extremely divergent and rely on a combination of individual methods, which usually provide spatially and temporally limited information on single parameters of interest. This review describes scanning ion conductance microscopy (SICM) as a novel versatile technique capable of simultaneously reporting various structural and functional parameters at nanometre resolution in living cardiovascular cells at the level of the whole tissue, single cells and at the subcellular level, to investigate the mechanisms of cardiovascular disease. SICM is a multimodal imaging technology that allows concurrent and dynamic analysis of membrane morphology and various functional parameters (cell volume, membrane potentials, cellular contraction, single ion-channel currents and some parameters of intracellular signalling) in intact living cardiovascular cells and tissues with nanometre resolution at different levels of organization (tissue, cellular and subcellular levels). Using this technique, we showed that at the tissue level, cell orientation in the inner and outer aortic arch distinguishes atheroprone and atheroprotected regions. At the cellular level, heart failure leads to a pronounced loss of T-tubules in cardiac myocytes accompanied by a reduction in Z-groove ratio. We also demonstrated the capability of SICM to measure the entire cell volume as an index of cellular hypertrophy. This method can be further combined with fluorescence to simultaneously measure cardiomyocyte contraction and intracellular calcium transients or to map subcellular localization of membrane receptors coupled to cyclic adenosine monophosphate production. The SICM pipette can be used for patch-clamp recordings of membrane potential and single channel currents. In conclusion, SICM provides a highly informative multimodal imaging platform for functional analysis of the mechanisms of cardiovascular diseases, which should facilitate identification of novel therapeutic strategies

High-resolution label-free 3D mapping of extracellular pH of single living cells

Abstract: Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells

Infection of Semen-Producing Organs by SIV during the Acute and Chronic Stages of the Disease

International audienceBACKGROUND: Although indirect evidence suggests the male genital tract as a possible source of persistent HIV shedding in semen during antiretroviral therapy, this phenomenon is poorly understood due to the difficulty of sampling semen-producing organs in HIV+ asymptomatic individuals. METHODOLOGY/PRINCIPAL FINDINGS: Using a range of molecular and cell biological techniques, this study investigates SIV infection within reproductive organs of macaques during the acute and chronic stages of the disease. We demonstrate for the first time the presence of SIV in the testes, epididymides, prostate and seminal vesicles as early as 14 days post-inoculation. This infection persists throughout the chronic stage and positively correlates with blood viremia. The prostate and seminal vesicles appear to be the most efficiently infected reproductive organs, followed by the epididymides and testes. Within the male genital tract, mostly T lymphocytes and a small number of germ cells harbour SIV antigens and RNA. In contrast to the other organs studied, the testis does not display an immune response to the infection. Testosteronemia is transiently increased during the early phase of the infection but spermatogenesis remains unaffected. CONCLUSIONS/SIGNIFICANCE: The present study reveals that SIV infection of the macaque male genital tract is an early event and that semen-producing organs display differential infection levels and immune responses. These results help elucidate the origin of HIV in semen and constitute an essential base to improving the design of antiretroviral therapies to eradicate virus from semen

Basic science232. Certolizumab pegol prevents pro-inflammatory alterations in endothelial cell function

Background: Cardiovascular disease is a major comorbidity of rheumatoid arthritis (RA) and a leading cause of death. Chronic systemic inflammation involving tumour necrosis factor alpha (TNF) could contribute to endothelial activation and atherogenesis. A number of anti-TNF therapies are in current use for the treatment of RA, including certolizumab pegol (CZP), (Cimzia ®; UCB, Belgium). Anti-TNF therapy has been associated with reduced clinical cardiovascular disease risk and ameliorated vascular function in RA patients. However, the specific effects of TNF inhibitors on endothelial cell function are largely unknown. Our aim was to investigate the mechanisms underpinning CZP effects on TNF-activated human endothelial cells. Methods: Human aortic endothelial cells (HAoECs) were cultured in vitro and exposed to a) TNF alone, b) TNF plus CZP, or c) neither agent. Microarray analysis was used to examine the transcriptional profile of cells treated for 6 hrs and quantitative polymerase chain reaction (qPCR) analysed gene expression at 1, 3, 6 and 24 hrs. NF-κB localization and IκB degradation were investigated using immunocytochemistry, high content analysis and western blotting. Flow cytometry was conducted to detect microparticle release from HAoECs. Results: Transcriptional profiling revealed that while TNF alone had strong effects on endothelial gene expression, TNF and CZP in combination produced a global gene expression pattern similar to untreated control. The two most highly up-regulated genes in response to TNF treatment were adhesion molecules E-selectin and VCAM-1 (q 0.2 compared to control; p > 0.05 compared to TNF alone). The NF-κB pathway was confirmed as a downstream target of TNF-induced HAoEC activation, via nuclear translocation of NF-κB and degradation of IκB, effects which were abolished by treatment with CZP. In addition, flow cytometry detected an increased production of endothelial microparticles in TNF-activated HAoECs, which was prevented by treatment with CZP. Conclusions: We have found at a cellular level that a clinically available TNF inhibitor, CZP reduces the expression of adhesion molecule expression, and prevents TNF-induced activation of the NF-κB pathway. Furthermore, CZP prevents the production of microparticles by activated endothelial cells. This could be central to the prevention of inflammatory environments underlying these conditions and measurement of microparticles has potential as a novel prognostic marker for future cardiovascular events in this patient group. Disclosure statement: Y.A. received a research grant from UCB. I.B. received a research grant from UCB. S.H. received a research grant from UCB. All other authors have declared no conflicts of interes