A global reference for human genetic variation

The 1000 Genomes Project set out to provide a comprehensive description of common human genetic variation by applying whole-genome sequencing to a diverse set of individuals from multiple populations. Here we report completion of the project, having reconstructed the genomes of 2,504 individuals from 26 populations using a combination of low-coverage whole-genome sequencing, deep exome sequencing, and dense microarray genotyping. We characterized a broad spectrum of genetic variation, in total over 88 million variants (84.7 million single nucleotide polymorphisms (SNPs), 3.6 million short insertions/deletions (indels), and 60,000 structural variants), all phased onto high-quality haplotypes. This resource includes >99% of SNP variants with a frequency of >1% for a variety of ancestries. We describe the distribution of genetic variation across the global sample, and discuss the implications for common disease studies

The environmental niche of the squid-jigging fleet in the North Pacific Ocean based on automatic identification system data

The vast number of fishing fleets and limited regulatory capacity pose challenges to the effective monitoring and management of marine fishery resources. To understand the fishing characteristics of spatial distribution for squid-jigging fleets in the North Pacific Ocean (NPO), satellite-based automatic identification system (AIS) data during 2016 ∼ 2020 and marine environmental data were applied to explore the drivers of fishing activities based on the boosted regression tree (BRT) and generalized additive model (GAM). The results showed that the BRT model has better performance than the GAM. The spatial distribution of squid-jigging fleets has a significant seasonality. Specifically, the spatial distribution of Fishing effort (FE) gradually shifted to the northwest from May to September, while gradually shifted to the southwest from October to November. The favourable area for focusing FE was the 150°E ∼ 165°E, 40°N ∼ 44°N. The latitudinal distribution of FE was not wide and the longitudinal distribution was long. Variations in the marine environment constantly influenced the FE of the squid-jigging fleets. Overall, the influence of sea surface temperature (T0) on FE was most significant. The favourable range of T0 for FE was 12 ∼ 20°C. Water temperature at the 300 m depth (T300) was also an important factor through the fishing season. The favourable range of T300 for FE was 3 ∼ 6°C. There were obvious differences in the influence of other environmental factors on FE in different months. Different environmental variables drive the spatial distribution of fishing effort in different months. This study may help to scientifically and effectively guide fishery management and sustainable development by evaluating the spatial variations in fishing activity

Tribological Properties of Tungsten Disulfide Nanoparticles Surface-Capped by Oleylamine and Maleic Anhydride Dodecyl Ester as Additive in Diisooctylsebacate

Oleylamine (OM) and maleic anhydride dodecyl ester (MADE, synthesized at our laboratory) were adopted as the surface modifiers to prepare OM/MADE-capped tungsten disulfide (WS₂) nanoparticles. An X-ray diffractometer and a transmission electron microscope were performed to analyze the microstructure and phase ingredients of the OM/MADE-capped WS₂ nanoparticles. Moreover, a four-ball friction and wear tester and a reciprocating tribometer were employed to evaluate the tribological properties of the surface-capped WS₂ nanoparticles as the lubricant additive in diisooctylsebacate (DIOS) from room temperature to 150 °C. The morphology of the worn steel surfaces and wear scars and their chemical states were investigated with a scanning electron microscope, three-dimensional profilometry, and an X-ray photoelectron spectroscope. Results show that OM-capped WS₂ nanoparticles nearly have no effect on the tribological properties of the DIOS base oil. The OM/MADE-capped WS₂ nanoparticles added in the same base stock at a concentration of 2.0% (mass fraction), however, exhibit good dispersibility and result in greatly improved tribological properties. The reason lies in that, after surface-capping by MADE containing polar group and OM containing coordination group, the OM/MADE-capped WS₂ particulates added in the base oil are well adsorbed on the sliding surfaces of the steel–steel contact to afford a chemisorption film with a low shear force. At the same time, OM/MADE-capped WS₂ nanoparticles as the additive in DIOS base oil take part in tribochemical reactions to form tribofilm composed of WO₃ and iron oxides on sliding surfaces, which also contributes to reducing the friction and wear of the steel sliding contact