Sprouty1 regulates reversible quiescence of a self-renewing adult muscle stem cell pool during regeneration.

Satellite cells are skeletal muscle stem cells capable of self-renewal and differentiation after transplantation, but whether they contribute to endogenous muscle fiber repair has been unclear. The transcription factor Pax7 marks satellite cells and is critical for establishing the adult satellite cell pool. By using a lineage tracing approach, we show that after injury, quiescent adult Pax7(+) cells enter the cell cycle; a subpopulation returns to quiescence to replenish the satellite cell compartment, while others contribute to muscle fiber formation. We demonstrate that Sprouty1 (Spry1), a receptor tyrosine kinase signaling inhibitor, is expressed in quiescent Pax7(+) satellite cells in uninjured muscle, downregulated in proliferating myogenic cells after injury, and reinduced as Pax7(+) cells re-enter quiescence. We show that Spry1 is required for the return to quiescence and homeostasis of the satellite cell pool during repair. Our results therefore define a role for Spry1 in adult muscle stem cell biology and tissue repair

Noradrenergic induction of odor-specific neural habituation and olfactory memories

For many mammals, individual recognition of conspecifics relies on olfactory cues. Certain individual recognition memories are thought to be stored when conspecific odor cues coincide with surges of noradrenaline (NA) triggered by intensely arousing social events. Such familiar stimuli elicit reduced behavioral responses, a change likely related to NA-dependent plasticity in the olfactory bulb (OB). In addition to its role in these ethological memories, NA signaling in the OB appears to be relevant for the discrimination of more arbitrary odorants as well. Nonetheless, no NA-gated mechanism of long-term plasticity in the OB has ever been directly observed in vivo. Here, we report that NA release from locus ceruleus (LC), when coupled to odor presentation, acts locally in the main OB to cause a specific long-lasting suppression of responses to paired odors. These effects were observed for both food odors and urine, an important social recognition cue. Moreover, in subsequent behavioral tests, mice exhibited habituation to paired urine stimuli, suggesting that this LC-mediated olfactory neural plasticity, induced under anesthesia, can store an individual recognition memory that is observable after recovery

Forecasting basketball players’ performance using sparse functional data

Statistics and analytic methods are becoming increasingly important in basketball. In particular, predicting players’ performance using past observations is a considerable challenge. The purpose of this study is to forecast the future behavior of basketball players. The available data are sparse functional data, which are very common in sports. So far, however, no forecasting method designed for sparse functional data has been used in sports. A methodology based on two methods to handle sparse and irregular data, together with the analogous method and functional archetypoid analysis is proposed. Results in comparison with traditional methods show that our approach is competitive and additionally provides prediction intervals. The methodology can also be used in other sports when sparse longitudinal data are available

Effect of religiosity/spirituality and sense of coherence on depression within a rural population in Greece: the Spili III project

Background: Recent research has addressed the hypothesis that religiosity/spirituality and sense of coherence buffer the negative effects of stress on numerous health issues. The aim of the current study was to further this work by exploring potential links between psycho-social factors such as religiosity/ spirituality and sense of coherence with depression. Methods: A total of 220 subjects of the SPILI III cohort (1988-2012) attending a primary care setting in the town of Spili on rural Crete represented the target group. All participants underwent a standardized procedure. Validated questionnaires were used to evaluate sense of coherence, depression levels and religious and spiritual beliefs. A multiple linear regression analysis of the Beck Depression Inventory Scale (BDI) in relation to demographic characteristics, scores on the Royal Free Interview for Spiritual and Religious Beliefs scale (RFI-SRB) and Sense of Coherence scale (SOC) was used. Results: A significant inverse association was found between BDI and RFI-SRB scale (B-coef=-0.6999, p<0.001), as well as among BDI and SOC scale (B-coef=-0.556, p<0.001). Conclusions: The findings of the current observational study indicate that highly religious participants are less likely to score high in the depression scale. Furthermore, participants with high SOC scored significantly low in the BDI scale. Further research is required in order to explore the potential effect of SOC and religiosity/spirituality in mental health

Sweating the small stuff: simulating dwarf galaxies, ultra-faint dwarf galaxies, and their own tiny satellites

We present FIRE/Gizmo hydrodynamic zoom-in simulations of isolated dark matter halos, two each at the mass of classical dwarf galaxies ($M_{\rm vir} \simeq 10^{10} M_{\odot}$) and ultra-faint galaxies ($M_{\rm vir} \simeq 10^9 M_{\odot}$), and with two feedback implementations. The resultant central galaxies lie on an extrapolated abundance matching relation from $M_{\star} \simeq 10^6$ to $10^4 M_{\odot}$ without a break. Every host is filled with subhalos, many of which form stars. Our dwarfs with $M_{\star} \simeq 10^6 M_{\odot}$ each have 1-2 well-resolved satellites with $M_{\star} = 3-200 \times 10^3 M_{\odot}$. Even our isolated ultra-faint galaxies have star-forming subhalos. If this is representative, dwarf galaxies throughout the universe should commonly host tiny satellite galaxies of their own. We combine our results with the ELVIS simulations to show that targeting $\sim 50~ \rm kpc$ regions around nearby isolated dwarfs could increase the chances of discovering ultra-faint galaxies by $\sim 35\%$ compared to random halo pointings, and specifically identify the region around the Phoenix dwarf galaxy as a good potential target. The well-resolved ultra-faint galaxies in our simulations ($M_{\star} \simeq 3 - 30 \times 10^3 M_{\odot}$) form within $M_{\rm peak} \simeq 0.5 - 3 \times 10^9 M_{\odot}$ halos. Each has a uniformly ancient stellar population ($> 10~ \rm Gyr$) owing to reionization-related quenching. More massive systems, in contrast, all have late-time star formation. Our results suggest that $M_{\rm halo} \simeq 5 \times 10^9 M_{\odot}$ is a probable dividing line between halos hosting reionization "fossils" and those hosting dwarfs that can continue to form stars in isolation after reionization.Comment: 12 pages, 6 figures, 1 table, submitted to MNRA

Noradrenergic plasticity of olfactory sensory neuron inputs to the main olfactory bulb

Sensory responses are modulated by internal factors including attention, experience, and brain state. This is partly due to fluctuations in neuromodulatory input from regions such as the noradrenergic locus ceruleus (LC) in the brainstem. LC activity changes with arousal and modulates sensory processing, cognition, and memory. The main olfactory bulb (MOB) is richly targeted by LC fibers and noradrenaline profoundly influences MOB circuitry and odor-guided behavior. Noradrenaline-dependent plasticity affects the output of the MOB; however. it is unclear whether noradrenergic plasticity also affects the input to the MOB from olfactory sensory neurons (OSNs) in the glomerular layer. Noradrenergic terminals are found in the glomerular layer, but noradrenaline receptors do not seem to acutely modulate OSN terminals in vitro. We investigated whether noradrenaline induces plasticity at the glomerulus. We used wide-field optical imaging to measure changes in odor responses following electrical stimulation of LC in anesthetized mice. Surprisingly, odor-evoked intrinsic optical signals at the glomerulus were persistently weakened after LC activation. Calcium imaging selectively from OSNs confirmed that this effect was due to suppression of presynaptic input and was prevented by noradrenergic antagonists. Finally, suppression of responses to an odor did not require precise coincidence of the odor with LC activation. However, suppression was intensified by LC activation in the absence of odors. We conclude that noradrenaline release from LC has persistent effects on odor processing already at the first synapse of the main olfactory system. This mechanism could contribute to arousal-dependent memories

Transferable Output ASCII Data (TOAD) editor version 1.0 user's guide

The Transferable Output ASCII Data (TOAD) editor is an interactive software tool for manipulating the contents of TOAD files. The TOAD editor is specifically designed to work with tabular data. Selected subsets of data may be displayed to the user's screen, sorted, exchanged, duplicated, removed, replaced, inserted, or transferred to and from external files. It also offers a number of useful features including on-line help, macros, a command history, an 'undo' option, variables, and a full compliment of mathematical functions and conversion factors. Written in ANSI FORTRAN 77 and completely self-contained, the TOAD editor is very portable and has already been installed on SUN, SGI/IRIS, and CONVEX hosts