A Rapid Molecular Approach for Chromosomal Phasing

Determining the chromosomal phase of pairs of sequence variants – the arrangement of specific alleles as haplotypes – is a routine challenge in molecular genetics. Here we describe Drop-Phase, a molecular method for quickly ascertaining the phase of pairs of DNA sequence variants (separated by 1-200 kb) without cloning or manual single-molecule dilution. In each Drop-Phase reaction, genomic DNA segments are isolated in tens of thousands of nanoliter-sized droplets together with allele-specific fluorescence probes, in a single reaction well. Physically linked alleles partition into the same droplets, revealing their chromosomal phase in the co-distribution of fluorophores across droplets. We demonstrated the accuracy of this method by phasing members of trios (revealing 100% concordance with inheritance information), and demonstrate a common clinical application by phasing CFTR alleles at genomic distances of 11–116 kb in the genomes of cystic fibrosis patients. Drop-Phase is rapid (requiring less than 4 hours), scalable (to hundreds of samples), and effective at long genomic distances (200 kb)

Bridging the gender divide: Facilitating the educational path for men in nursing

Background: Although the number of men entering the nursing profession over the past century has increased in-crementally, the proportion of men remains low in contrast to the U.S. population. On matriculation into nursing school, men face stereotypes about the nursing profession and the characteristics of the men who enter it. Men may also face a number of gender-based barriers, including lack of history about men in nursing, lack of role models, role strain, gender discrimination, and isolation. Method: This article describes each of these barriers and provides strategies to improve male students’ learning experience. Results: The efforts of one nursing school to address many of these barriers are also described. Conclusion: Through acknowledging gender barriers and taking intentional steps to address them with prenursing and nursing students, schools of nursing may create a more inclusive environment and enhance the profession’s diversity

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Mapping Benthic Algae and Cyanobacteria in River Channels from Aerial Photographs and Satellite Images: A Proof-of-Concept Investigation on the Buffalo National River, AR, USA

Although rivers are of immense practical, aesthetic, and recreational value, these aquatic habitats are particularly sensitive to environmental changes. Increasingly, changes in streamflow and water quality are resulting in blooms of bottom-attached (benthic) algae, also known as periphyton, which have become widespread in many water bodies of US national parks. Because these blooms degrade visitor experiences and threaten human and ecosystem health, improved methods of characterizing benthic algae are needed. This study evaluated the potential utility of remote sensing techniques for mapping variations in algal density in shallow, clear-flowing rivers. As part of an initial proof-of-concept investigation, field measurements of water depth and percent cover of benthic algae were collected from two reaches of the Buffalo National River along with aerial photographs and multispectral satellite images. Applying a band ratio algorithm to these data yielded reliable depth estimates, although a shallow bias and moderate level of precision were observed. Spectral distinctions among algal percent cover values ranging from 0 to 100% were subtle and became only slightly more pronounced when the data were aggregated to four ordinal levels. A bagged trees machine learning model trained using the original spectral bands and image-derived depth estimates as predictor variables was used to produce classified maps of algal density. The spatial and temporal patterns depicted in these maps were reasonable but overall classification accuracies were modest, up to 64.6%, due to a lack of spectral detail. To further advance remote sensing of benthic algae and other periphyton, future studies could adopt hyperspectral approaches and more quantitative, continuous metrics such as biomass

How Robust is the Relationship between Financial Intermediation and Economic Growth?

The question of whether financial intermediation has a first order effect on the development process has long been debated. There have also been questions about the ‘robustness’ of the empirical results that suggest financial development does indeed have a first order effect. This paper addresses the second issue within this debate by assessing the robustness of the link between financial development and economic growth to variations in the sample (countries included in the data set). Specifically, the procedure identifies the relative influence of ordered subsets in the data to determine whether or not these financial variables change sign or lose statistical significance after the removal a specific subset. The results of this exercise suggest that financial intermediation appears to have a first order effect, that is most of the variables are robust to variations in the sample, using a traditional cross-country growth regression framework and an instrumental variables framework (GMM).Financial Development, Economic Growth, Sensitivity Analysis

Comparisons of Twelve Freshwater Mussel Bed Assemblages Quantitatively Sampled at a 15-year Interval in the Buffalo National River, Arkansas, USA

Historically, 23 freshwater mussel species have been documented from the Buffalo National River (BNR), a 246 km, free-flowing river in northern Arkansas. The potential threats to BNR include land use/land cover changes, eutrophication, recreation, physical habitat changes, and various climate change-related effects. Twelve quantitative mussel bed sites were established and then sampled using a stratified random sampling protocol to evaluate the long-term changes between 2006 and 2020–2021 in population and assemblage characteristics. We compared (1) overall mussel bed persistence, sampling confidence levels and study-wide relative abundances, and compared species’ size and size-frequency distributions; (2) 10 overall site assemblage variables using paired t-tests; (3) site-level mean density, richness, and diversity indices using pair-wise Mann–Whitney U statistics; and (4) assemblage composition using Non-Metric Multidimensional Scaling. The major findings included the following: (1) sampling efforts based on a targeted 80% confidence level appears relatively robust, (2) BNR mussel assemblage composition and structure were relatively stable (however, small mussel bed persistence is a concern), (3) 7 of 23 sites were outliers based on freshwater mussel composition and habitat characteristics, and (4) assemblage composition changed with three species declining (Actinonaias ligamentina, Lasmigona costata, and Ptychobranchus occidentalis) and four species increasing (Cambarunio hesperus, Cyclonaias tuberculata, Eurynia dilatata, and Venustaconcha pleasii) between monitoring events

New Concepts in the 2014 \u3cem\u3eACA Code of Ethics\u3c/em\u3e

The 2014 revision of the ACA Code of Ethics (American Counseling Association [ACA], ) substantially raises the bar for the ethical practice of professional counselors. This article provides interviews with members of the ACA Ethics Revision Task Force that explore and clarify new imperatives in the areas of ethical decision making, professional values, managing and maintaining boundaries, technology (including social media), the nonimposition of counselor personal values, counselor education, legal issues, sliding scales, and fee splitting

How are competitive mountain bikers training: an assessment of frequencies, modalities, and durations

The popularity of mountain biking has increased steadily over the past 10 years, and the number of competitive mountain bikers increases on a yearly basis. High physical conditioning is essential in performing well within the sport. As important as the training component is, very little is known as to how competitive mountain biker's train in an effort to enhance performance. Physiological parameters associated with success within the sport of mountain biking have been identified, as well as training suggestions for performance enhancement. However, these training suggestions targeted towards mountain bikers are not data driven. For the purposes of the present study, frequencies, durations, and modalities of exercise training among competitive mountain bikers were assessed, as well as recovery. A clear theoretical model was created to provide insight into how competitive mountain bikers are training. This model guided the present investigation and helped determine whether or not competitive mountain bikers are incorporating training techniques specifically targeted towards improving the physiological characteristics associated with success within the sport of mountain biking. Forty competitive riders completed a one-time in-depth online survey designed for the purposes of this study. The development of the present survey was driven by exercise training modalities known to increase the performance parameters associated with successful mountain bike riding. Participants represented all regions of the United States and were recruited through university sponsored sports club teams and NCAA intercollegiate teams. Competitive riders affiliated with local and national racing chapters were also contacted as potential participants. Results from the present study suggest that riders are participating in training modalities shown to increase physiological markers associated with successful mountain bike riding. Additionally, a majority of riders are adhering to the recommendations of workout frequency, as well as the recommended duration of these workout sessions. Findings from the current study also suggest that riders spend a majority of their exercise training on the trail. (Published By University of Alabama Libraries